BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	648,130	A→T	7.0%	L435L (CTA→CTT)	EO946_RS03395 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	648,130	0	A	T	7.0%	689.4 / 33.1	211	L435L (CTA→CTT)	EO946_RS03395	MFS transporter
Reads supporting (aligned to +/- strand): ref base A (78/115); new base T (7/8); total (87/124)
Fisher's exact test for biased strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 220 total aligned reads displayed.

TGTGGCGTTAGCCGGGTCATTTTCGGCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCAGAAAGTAA  >  NZ_CP034943/647995‑648267

tgtgGCGTTAGCCGGGTCATTTTCGGCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGctatcta                                                                                                                                       <  2:270432/140‑1 (MQ=255)

tggggCGTGAGCCGGGTCATTTTCTGCCATCTATTTAGGAATCGCAGGATATGTAGAGAATGCAGACCAGTCAGTTACGTCATTGAATGCGATTAAAAACAGGAATGCTTTGATTCCGGGATTTTTCATTTTGctatcta                                                                                                                                       <  2:1977285/137‑1 (MQ=255)

      gTTAGCCGGGTCATTTTCGGCCATCGATTTAGGAATCGCAGGAGATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAAGGGGAGTACAAGCGTGAATGCGTTGGGGCCGGGCTGTTTCAGTGTGCTCTCTCttcttc                                                                                                                                 >  1:335511/1‑140 (MQ=255)

      gTTAGCCGGGTCATTTTCGGCCATCGATGTAGGAATCGCAGGAGATGTAGCGACTGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATtc                                                                                                                                 >  1:2687427/1‑140 (MQ=255)

        tAGCCGGGTCATTTTCGGCCATTTATTTAGGAATCGCCGGGTATGTGGCGAATACAGACCAGACAGTTGCGTCGTTGAATGCGATTAAAAATATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTTTCTATTATtctt                                                                                                                               >  2:2531427/1‑139 (MQ=255)

        tAGCCGGGTCATTTTCGGCCATTTATTTAGGAATCGCCGGGTATGTGGCGAATACAGACCAGACAGTTGCGTCGTTGAATGCGATTAAAAATATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTTTCTATTATtctt                                                                                                                               >  2:450314/1‑139 (MQ=255)

        tAGCCGGGTCATTTTCGGCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATtctc                                                                                                                               >  2:3415638/1‑140 (MQ=255)

        tAGCCGGGTCATTTTCGGCCATCTATGTAGGACTCGCAGGATATGTCGCGAATGCAGACCAGACAGGTACGTCATTGAATGCGATTAAAAACATGACTGCTTTGATTCCGGGATTTGTCATTTTGCTATCTATTATtctc                                                                                                                               >  2:1542415/1‑140 (MQ=255)

          gCCGGGTCATTTTCGGCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCAt                                                                                                                             >  1:415573/1‑140 (MQ=255)

          gCCGGGTCATTTTCGGCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCAt                                                                                                                             <  1:1853847/140‑1 (MQ=255)

           ccGGGTCATTTTCGGCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATc                                                                                                                            <  2:2773209/140‑1 (MQ=255)

             gggTCATTTTCGGCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGc                                                                                                                          <  2:817438/140‑1 (MQ=255)

              ggTCATTTTCGGCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGcc                                                                                                                         <  2:3291654/140‑1 (MQ=255)

              ggTCATTTTCGGCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGcc                                                                                                                         <  2:2624577/140‑1 (MQ=255)

              ggTCATTTTCGGCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGATTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGcc                                                                                                                         <  2:1317507/140‑1 (MQ=255)

              ggTCATTTTCGGCAATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGcc                                                                                                                         <  2:3592172/140‑1 (MQ=255)

                tCATTGTCGGGCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCtt                                                                                                                       <  2:3061447/140‑1 (MQ=255)

                         gCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCttt                                                                                                              >  2:3834918/1‑140 (MQ=255)

                         gCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCttt                                                                                                              >  1:1975765/1‑140 (MQ=255)

                              cTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTAAGTCATTGAATGCGATTAAAAACATGAATGCTTTTATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCATTTAACgg                                                                                                         >  1:2409288/1‑140 (MQ=255)

                                  ttAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGaaaa                                                                                                     <  1:2054309/140‑1 (MQ=255)

                                  ttAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCAGTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGaaaa                                                                                                     <  1:785287/140‑1 (MQ=255)

                                  ttAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGAGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATGCTCATCGCCTTCTATCCTTTAACGGaaaa                                                                                                     <  1:3131964/140‑1 (MQ=255)

                                  ttAGGAATCGCAGGATATGGAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGaaaa                                                                                                     <  1:1389457/140‑1 (MQ=255)

                                  ttAGGAATCGCAGGATAGGTAGCGAATGCAGACCAGACAGTTTCTTCATTGAATGCTATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCATTCTATCCTTTAACGGaaaa                                                                                                     <  2:3231625/140‑1 (MQ=255)

                                  tgaGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGaaaa                                                                                                     <  1:3492435/138‑1 (MQ=255)

                                         tCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGatat                                                                                              <  2:3618706/140‑1 (MQ=255)

                                         tCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGatat                                                                                              >  2:834345/1‑140 (MQ=255)

                                           gCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGaaaa                                                                                                     <  1:1376307/131‑1 (MQ=255)

                                           gCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGaaaa                                                                                                     >  2:1376383/1‑131 (MQ=255)

                                            cAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAg                                                                                           <  1:1558550/140‑1 (MQ=255)

                                            cAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTAAGGGATTTTTCATTTTGATATCTATTATTATAATCGCCTTCTATCATTTAACGGAAAAAAGATATaaa                                                                                           >  1:1018087/1‑139 (MQ=255)

                                               gATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGcac                                                                                        >  2:325180/1‑140 (MQ=255)

                                               gATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGcac                                                                                        >  2:3294487/1‑140 (MQ=255)

                                               gATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGcac                                                                                        >  2:1592629/1‑140 (MQ=255)

                                               gATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGcac                                                                                        >  2:668794/1‑140 (MQ=255)

                                                 tatGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCAcat                                                                                      <  2:3091835/140‑1 (MQ=255)

                                                 tatGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCAcat                                                                                      <  2:1040324/140‑1 (MQ=255)

                                                   tGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGaaaa                                                                                                     >  2:190497/1‑123 (MQ=255)

                                                    gTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCAcatcat                                                                                   >  1:1052735/1‑140 (MQ=255)

                                                    gTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATACTTTAACGGAAAAAAGATATAAGCAcatcat                                                                                   >  1:6809/1‑140 (MQ=255)

                                                     tAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACatcatc                                                                                  >  1:1284442/1‑140 (MQ=255)

                                                         gAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTg                                                                              >  2:1803265/1‑140 (MQ=255)

                                                         gAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTg                                                                              >  1:3592651/1‑140 (MQ=255)

                                                         gAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTg                                                                              >  1:3592921/1‑140 (MQ=255)

                                                          aaTGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGa                                                                             >  2:759169/1‑140 (MQ=255)

                                                          aaTGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGa                                                                             >  1:2780052/1‑140 (MQ=255)

                                                          aaTGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGa                                                                             >  2:1176868/1‑140 (MQ=255)

                                                           aTGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATCTAAGCACATCATCTCTGAg                                                                            >  2:2998316/1‑140 (MQ=255)

                                                           aTGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAg                                                                            >  1:1466332/1‑140 (MQ=255)

                                                            tGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTc                                                                                                                      <  1:2654000/97‑1 (MQ=255)

                                                            tGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGc                                                                           <  2:269927/140‑1 (MQ=255)

                                                            tGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGc                                                                           <  1:834345/140‑1 (MQ=255)

                                                            tGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGc                                                                           <  2:1125844/140‑1 (MQ=255)

                                                             gCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCt                                                                          <  1:3626517/140‑1 (MQ=255)

                                                             gCAGACCAGACAGGTCCGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCt                                                                          <  1:3143356/140‑1 (MQ=255)

                                                                 accagacAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAATAGATATAAGCACATCATCTCTGAGCTGGAg                                                                      >  1:2253855/1‑140 (MQ=255)

                                                                  ccagacAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGc                                                                     <  1:388655/140‑1 (MQ=255)

                                                                  ccagacAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGc                                                                     <  1:231905/140‑1 (MQ=255)

                                                                  ccagacAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGc                                                                     <  1:3556075/140‑1 (MQ=255)

                                                                    agacAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCaa                                                                   <  1:2366675/140‑1 (MQ=255)

                                                                          ttACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAg                                                                                           >  2:621502/1‑110 (MQ=255)

                                                                           tACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTg                                                            <  2:2975659/140‑1 (MQ=255)

                                                                           tACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTg                                                            >  1:1489821/1‑140 (MQ=255)

                                                                             cGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCa                                                                                                                              <  2:2002464/72‑1 (MQ=255)

                                                                             cGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCa                                                                                                                              >  1:2002464/1‑72 (MQ=255)

                                                                             cGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGcc                                                          >  1:2998498/1‑140 (MQ=255)

                                                                                cATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGa                                                       <  2:371690/140‑1 (MQ=255)

                                                                                cATTGAATGCGATTAAAAACATGAAGGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGa                                                       <  2:1704447/140‑1 (MQ=255)

                                                                                  ttGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATa                                                     >  2:430233/1‑140 (MQ=255)

                                                                                  ttGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATa                                                     >  2:446744/1‑140 (MQ=255)

                                                                                  ttGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATa                                                     >  1:1118866/1‑140 (MQ=255)

                                                                                  ttGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATa                                                     >  2:1625450/1‑140 (MQ=255)

                                                                                  ttGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATa                                                     >  2:2211312/1‑140 (MQ=255)

                                                                                           aTTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCGGGAGCAAAGGTCTGCCAGATAAATATATGt                                            >  2:1854001/1‑140 (MQ=255)

                                                                                           aTTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATAAAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGt                                            >  2:260851/1‑140 (MQ=255)

                                                                                                            tttGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCa                           <  2:2880288/140‑1 (MQ=255)

                                                                                                            tttGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCa                           <  1:3702914/140‑1 (MQ=255)

                                                                                                            tttGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCa                           <  1:2211312/140‑1 (MQ=255)

                                                                                                            tttGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATAGTCa                           <  1:446744/140‑1 (MQ=255)

                                                                                                               gATTCCGGGATTTTTCATTTTGCTATCTATTAGTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAAtg                        <  2:2122966/140‑1 (MQ=255)

                                                                                                                 ttCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAAtgtt                      <  2:127726/140‑1 (MQ=255)

                                                                                                                 ttCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAAtgtt                      <  2:415573/140‑1 (MQ=255)

                                                                                                                             tCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCa          <  1:3257428/140‑1 (MQ=255)

                                                                                                                             tCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCa          <  1:6545/140‑1 (MQ=255)

                                                                                                                             tCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCa          <  2:529504/140‑1 (MQ=255)

                                                                                                                             tCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCa          <  1:3788604/140‑1 (MQ=255)

                                                                                                                             tCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCa          <  1:1846750/140‑1 (MQ=255)

                                                                                                                             tCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCa          <  1:3455157/140‑1 (MQ=255)

                                                                                                                             tCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGAAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCa          <  2:2409288/140‑1 (MQ=255)

                                                                                                                             tCATTTTGCTATCTATTAGTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCa          <  1:610759/140‑1 (MQ=255)

                                                                                                                               aTTTTGCTTTCTATTATTCTTATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAACACATCATCTCTGAGCTGGAGCAAAGGTCTGCGAGATAAATAGATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCaaa        <  1:2531634/140‑3 (MQ=255)

                                                                                                                               aTTTTGCTTTCTATTATTCTTATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAACACATCATCTCTGAGCTGGAGCAAAGGTCTGCGAGATAAATAGATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCaaa        <  1:2530541/140‑3 (MQ=255)

                                                                                                                               aTTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCAGa        <  2:2349595/140‑1 (MQ=255)

                                                                                                                               aTTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCAGa        <  2:3652018/140‑1 (MQ=255)

                                                                                                                               aTTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCAGa        <  1:1136966/140‑1 (MQ=255)

                                                                                                                               aTTTTGCTATCGATTATTCTCCTCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCCATGTTGTATTTATGGCAGa        <  1:498919/140‑1 (MQ=255)

                                                                                                                                 tttGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCAGaaa      <  2:1489821/140‑1 (MQ=255)

                                                                                                                                     ctatctatTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCAGAAAGTaa  <  2:3779321/140‑1 (MQ=255)

                                                                                                                                     ctatctatTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCAGAAAGTaa  <  2:3528633/140‑1 (MQ=255)

TGTGGCGTTAGCCGGGTCATTTTCGGCCATCTATTTAGGAATCGCAGGATATGTAGCGAATGCAGACCAGACAGTTACGTCATTGAATGCGATTAAAAACATGAATGCTTTGATTCCGGGATTTTTCATTTTGCTATCTATTATTCTCATCGCCTTCTATCCTTTAACGGAAAAAAGATATAAGCACATCATCTCTGAGCTGGAGCAAAGGTCTGCCAGATAAATATATGTAAAATTTTTAATATTCAATGTTGTATTTATGGCAGAAAGTAA  >  NZ_CP034943/647995‑648267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`