Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 755,531 A→C 10.5% Q22H (CAA→CAC EO946_RS03880 → rhamnogalacturonan acetylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943755,5310AC10.5% 442.2 / 7.5 182Q22H (CAA→CACEO946_RS03880rhamnogalacturonan acetylesterase
Reads supporting (aligned to +/- strand):  ref base A (122/32);  new base C (2/23);  total (125/57)
Fisher's exact test for biased strand distribution p-value = 6.25e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

ACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCC  >  NZ_CP034943/755393‑755665
                                                                                                                                          |                                                                                                                                      
aCCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAg                                                                                                                                       <  1:398753/140‑1 (MQ=255)
aCCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAg                                                                                                                                       >  2:3557182/1‑140 (MQ=255)
           cAAGGGATGAGCTTTCCATTATTGATGCCATATTAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATGTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTggggg                                                                                                                            <  2:135357/140‑1 (MQ=255)
                   gagCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCACACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTtcg                                                                                                                    >  2:3214374/1‑139 (MQ=255)
                        ttCCATGAATGATGCCATATGAAACGAAAAAAACGAGGGGTAATGAGAAATGTAAAATCACTTTTATCTGGCTGGCTCTTCAACTCTTCAAACGTATGGAGAAAGCACACATCAAGGGGGGTGGTGTCAGTTTCTCGGCt                                                                                                               <  2:2629122/139‑1 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                              >  1:929811/1‑140 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                              >  2:1078078/1‑140 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                              >  1:1262297/1‑140 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                              >  2:1198562/1‑140 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                              >  1:173082/1‑140 (MQ=255)
                              gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGTCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATc                                                                                                         >  1:1422883/1‑140 (MQ=255)
                              gattgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAACCGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCa                                                                                                           <  2:97125/138‑1 (MQ=255)
                                ttgatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTg                                                                                                       >  2:2900179/1‑140 (MQ=255)
                                 tgatGCCATATCAAACGAAAAAAACGCGGGGGAATGAGTAATGTCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACACATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGc                                                                                                      <  2:2255175/140‑1 (MQ=255)
                                  tatGCAATATGAAACGAAACAAACTATGGTGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGACACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGcc                                                                                                     <  2:475889/139‑1 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGACTGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                    >  2:1543138/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGGAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAAGGTATTGAGACAGCACAAATCATGTGGGGTGGGGGCAGTTTCTCTGCTCGAATCTGCCg                                                                                                    >  2:2163662/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACCTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                    >  1:1631092/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTCTCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                    >  2:2681289/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                    >  1:2663975/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                    >  2:1686886/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                    >  1:3416212/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                    >  2:1430108/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                    >  2:3839016/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                    >  2:294112/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                    >  2:494285/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                    >  1:544732/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCAAAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                    >  2:328941/1‑140 (MQ=255)
                                     gCCATATGAACCGAAAAAAACGAGGGGGAATGAGAAATGCCAAATCACATTTATCTTGCTGCCGATTCAACTGTTCAAACGTATGGCGACAGCACAAACCACGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                  <  1:3555574/140‑1 (MQ=255)
                                         tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGTGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGaccct                                                                                              >  2:2217938/1‑136 (MQ=255)
                                         tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                              >  2:3484572/1‑140 (MQ=255)
                                             aaaataaaaaatCTATGGGTAATGATATATTTCAAATCACTTTTATCTTGCTTGCGATTCTACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                          <  2:2360500/135‑1 (MQ=255)
                                             aaaCTAACAACACGAGGGGGAATGAGAAATGGCAAATCACTTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                          <  2:3119765/140‑1 (MQ=255)
                                             aaaCTAAATAATCTAGGGGTAATGAGTATTTGCAAATCAGATTTTTATTGCTGGCGATTCAACTGTTAATACGTATGGAGACAGCAAAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                          <  2:2427385/140‑1 (MQ=255)
                                               actacaaaaaaGAGGGGGAATGATATATGTAAAATCACATTTATCTTGCTGGCGATTCAAGTGTTCAACAGTATGGAGCTATCACAAATCCAGGGCCGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATTTTCaa                                                                                        <  2:1280783/135‑1 (MQ=255)
                                               accaaaaaaaCGAGGGGGACTGAGCAATGCCAAATCACATTTATCTTGCTGGCGACCCAACTGCTCAAACGTATGCAGACACCACAACCCCCGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                        <  1:1585577/137‑1 (MQ=255)
                                                       accGAGCGCGAACGAGAAATCGCAAATCACATTTATCTCGCTGGCGATCCACCTCTCCAACCGTATGGAGACAGCCCAACTCCAGGCGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTaa                                                                                <  2:877064/138‑1 (MQ=255)
                                                            gtgtgAATGAGAAATTGCAAATCATTTTTATCTTGCTGGCGATTCAACTTTTCAAACGTATGGACACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACagag                                                                           <  2:2972385/136‑1 (MQ=255)
                                                            gggggAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGAGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACagag                                                                           <  2:3562407/140‑1 (MQ=255)
                                                                caaTGAGAACTCCCAAATCCCATTTATCTTGCGGGCCATACAACTCATCAACCCCATCGAGACAGCCCAAATCCCGGCGGCAGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAAt                                                                       <  2:1786349/139‑1 (MQ=255)
                                                                 aaTGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATc                                                                      >  2:1895403/1‑140 (MQ=255)
                                                                 aaTGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATc                                                                      >  2:1303737/1‑140 (MQ=255)
                                                                          tGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaaga                                                             >  1:100886/1‑140 (MQ=255)
                                                                          tGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaaga                                                             >  1:951040/1‑140 (MQ=255)
                                                                          tGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaaga                                                             >  1:2710348/1‑140 (MQ=255)
                                                                               aaTCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCACTCGGGGGACGAAGCTc                                                        >  1:1213079/1‑140 (MQ=255)
                                                                               aaTCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGACGAAGCTc                                                        >  1:1543994/1‑140 (MQ=255)
                                                                                  cacaTTTATCTTGCTGTCTATTCACCTGTTCAAACGTATGGACACAGCACACATCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGaa                                                     <  2:1027067/140‑1 (MQ=255)
                                                                                     aTTTATCTTGCTGGCGATTCAACGGCTCAAACGTACCGCCACAGCACACATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCACGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAAc                                                  <  1:377651/140‑1 (MQ=255)
                                                                                         aTCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTtct                                                                                                                    >  2:1939320/1‑70 (MQ=255)
                                                                                               ctgtctaTTCTACTGTTGAAAGGTTTGGAGACAGCAATATTGTTGGGGGGTGGGGTCAGTTTGTCGGCTCGCATCTGGCGGATCATATTCAAGTTATTAACAGAGAAATCGGGGGAATAAGCTCGAAAACATTTGTGGaa                                        <  2:1969322/134‑1 (MQ=255)
                                                                                               cTGGCGATTCAACTGTTCAAACGTATGGTGCCCGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                        <  2:3680195/140‑1 (MQ=255)
                                                                                               cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                        >  1:470676/1‑140 (MQ=255)
                                                                                               cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                        >  1:1909305/1‑140 (MQ=255)
                                                                                               cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                        >  2:1399815/1‑140 (MQ=255)
                                                                                               cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                        >  1:1150488/1‑140 (MQ=255)
                                                                                               cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                        >  1:39100/1‑140 (MQ=255)
                                                                                               cTGGCGATTCAACAGGTCAAACGTCTGGACCCACCACAACTCCCGGGGGGGGCGCGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                        <  1:2900179/140‑1 (MQ=255)
                                                                                                              ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGCAGAAGCTCGACAACATTTGTGGAAGAGGGCAGGCTTCAg                         >  1:2961200/1‑140 (MQ=255)
                                                                                                              ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGACGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                         >  2:1760161/1‑140 (MQ=255)
                                                                                                              ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                         >  2:648127/1‑140 (MQ=255)
                                                                                                              ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                         >  2:3413596/1‑140 (MQ=255)
                                                                                                              ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                         >  1:3655833/1‑140 (MQ=255)
                                                                                                              ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACAGTTGTGGAAGAGGGCAGGCTTCAg                         >  2:231614/1‑140 (MQ=255)
                                                                                                                taaaCGTATGGATACAGCACAACTCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGGATATTAAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                       <  2:2086930/139‑1 (MQ=255)
                                                                                                                   aCGTATGGAGACAGCACAAATCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGAt                    >  1:3693085/1‑140 (MQ=255)
                                                                                                                        tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg               >  1:3331534/1‑140 (MQ=255)
                                                                                                                        tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg               >  1:2022593/1‑140 (MQ=255)
                                                                                                                        tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg               >  1:2239012/1‑140 (MQ=255)
                                                                                                                        tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg               >  1:1360931/1‑140 (MQ=255)
                                                                                                                             aCAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAtgtg          >  1:1751296/1‑140 (MQ=255)
                                                                                                                             aCAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAtgtg          >  1:2431365/1‑140 (MQ=255)
                                                                                                                             aCAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAtgtg          >  1:813300/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTTGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt        >  1:2107410/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGAt                                                                                   <  2:93882/65‑1 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGACGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt        >  1:3739292/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt        >  2:445218/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt        >  1:2783195/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt        >  2:2614368/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt        >  1:2194139/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt        >  1:310394/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt        >  2:3345124/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt        >  1:3741785/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt        >  2:56719/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt        >  1:1773297/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGAGTCTTGATGTGAt        >  1:2314192/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATGTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGGGAt        >  1:3372714/1‑140 (MQ=255)
                                                                                                                                gCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAtt       >  1:347257/1‑140 (MQ=255)
                                                                                                                                 cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGTAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg      >  1:3199240/1‑140 (MQ=255)
                                                                                                                                 cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg      >  1:1119029/1‑140 (MQ=255)
                                                                                                                                 cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg      >  2:3792853/1‑140 (MQ=255)
                                                                                                                                 cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg      >  1:1794416/1‑140 (MQ=255)
                                                                                                                                 cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg      >  1:3035340/1‑140 (MQ=255)
                                                                                                                                 cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg      >  1:3045773/1‑140 (MQ=255)
                                                                                                                                 cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg      >  1:3856266/1‑140 (MQ=255)
                                                                                                                                 cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg      >  1:671081/1‑140 (MQ=255)
                                                                                                                                 cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCACGCGATTCTTGATGTGATTg      >  2:1985610/1‑140 (MQ=255)
                                                                                                                                     aaTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGcc  >  1:98074/1‑140 (MQ=255)
                                                                                                                                     aaTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGcc  >  1:747099/1‑140 (MQ=255)
                                                                                                                                     aaTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGcc  >  1:60932/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                      
ACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCC  >  NZ_CP034943/755393‑755665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: