Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 892,198 G→A 5.4% P283P (CCG→CCA EO946_RS04510 → iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943892,1980GA 5.4% 669.9 / 18.1 205P283P (CCG→CCAEO946_RS04510iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (66/127);  new base A (5/6);  total (71/134)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 205 total aligned reads displayed.

TTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTACATCGCAAGAAG  >  NZ_CP034943/892065‑892335
                                                                                                                                     |                                                                                                                                         
ttGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTcc                                                                                                                                     <  2:2160019/140‑1 (MQ=255)
 tgtgATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCg                                                                                                                                    <  2:1555631/140‑1 (MQ=255)
 tgtgATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCg                                                                                                                                    <  2:877554/140‑1 (MQ=255)
 tgtgATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCg                                                                                                                                    <  2:2461994/140‑1 (MQ=255)
 tgtgATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCg                                                                                                                                    <  2:865407/140‑1 (MQ=255)
         cACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCTCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTg                                                                                                                            >  2:2783940/1‑140 (MQ=255)
                     tcatGCTTGCAGGGTCCGCTGTCGCCATTGCCGGGCCGATTTCTTTTATCGGCATCATTATTCCGCATTTTGCCCGCTTTGTCGTTGGTAATGATTATCGCTGGGTTCTGCCATTTTCCGCTGTTTTGGGTGCCATTTTg                                                                                                                >  1:3245297/4‑140 (MQ=255)
                     tcatGCTTGCAGGGTCCGCTGTCGCCATTGCCGGGCCGATTTCTTTTATCGGCATCATTATTCCGCATTTTGCCCGCTTTGTCGTTGGTAATGATTATCGCTGGGTTCTGCCATTTTCCGCTGTTTTGGGTGCCATTTTg                                                                                                                >  1:1546164/4‑140 (MQ=255)
                     tcatGCTTGCAGGGTCCGCTGTCGCCATTGCCGGGCCGATTTCTTTTATCGGCATCATTATTACGCATTTTGCCCGCTTTGTCGTTGGTAATGATTATCGCTGGGTTCTGCCATTTTCCGCTGTTTTGGGTGCCATTTTg                                                                                                                >  1:3826605/4‑140 (MQ=255)
                       ttgcttgcCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTAGTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCt                                                                                                              <  2:2425325/140‑1 (MQ=255)
                       ttGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCt                                                                                                              <  1:1819554/140‑1 (MQ=255)
                          cTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGt                                                                                                           <  2:1013346/140‑1 (MQ=255)
                            aGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAg                                                                                                         <  1:2951714/140‑1 (MQ=255)
                               cGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAgctg                                                                                                      >  2:3248522/1‑140 (MQ=255)
                               cGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAgctg                                                                                                      >  1:249953/1‑140 (MQ=255)
                                gggTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAgctgc                                                                                                     <  2:3094522/140‑1 (MQ=255)
                                gggTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAgctgc                                                                                                     <  2:196850/140‑1 (MQ=255)
                                gggTCTGCGGTCGCACGTGCCGGGCCGATTTCATTGAGTGGCATCATTAGTCCGCCTGGTGGCCGATTTATCGGCGGCAATGAGTATCGTTGGGTTCTGCCGTTTTCCGCTGTTGTGGGTGCGATTGTGCTTGTAgctgc                                                                                                     <  2:678233/140‑1 (MQ=255)
                                           cGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCgggg                                                                                          <  1:2142820/140‑1 (MQ=255)
                                            gCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGgc                                                                                         >  1:890173/1‑140 (MQ=255)
                                             cAATTGCGGGGCCGATTTCATTTATTGTCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTGGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGgcg                                                                                        <  2:1634990/140‑1 (MQ=255)
                                             cAATTGCCGGTCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGgcg                                                                                        <  2:3541/140‑1 (MQ=255)
                                             cAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGgcg                                                                                        <  2:3005719/140‑1 (MQ=255)
                                                 tGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGAt                                                                                    >  2:480787/1‑140 (MQ=255)
                                                  gCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGAtt                                                                                   >  2:3162434/1‑140 (MQ=255)
                                                  gCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGAtt                                                                                   <  1:2799539/140‑1 (MQ=255)
                                                  gCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGAtt                                                                                   <  1:2190692/140‑1 (MQ=255)
                                                      ggCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGtttt                                                                                                                             <  1:1208833/94‑1 (MQ=255)
                                                      ggCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGtttt                                                                                                                             >  2:1208833/1‑94 (MQ=255)
                                                      ggCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGGTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATc                                                                               >  1:3048083/1‑140 (MQ=255)
                                                         ctaTTTCATTTATTGGCATCATTATTCCGCATTTTGCACGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCTCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCAtt                                                                            <  1:685837/138‑1 (MQ=255)
                                                         cGATTTCATTTATGGGCATCATTAGTCCGAATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTACGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCAtt                                                                            <  1:2807772/140‑1 (MQ=255)
                                                           aTTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTAt                                                                          <  2:1721054/140‑1 (MQ=255)
                                                           aTTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTAt                                                                          <  2:1461280/140‑1 (MQ=255)
                                                             ttCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATAGGGGCGCGATTTATCATTATGc                                                                        >  1:2051681/1‑140 (MQ=255)
                                                                 tttATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCa                                                                    >  2:3217195/1‑140 (MQ=255)
                                                                    aTTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGa                                                                 >  2:3828311/1‑140 (MQ=255)
                                                                      tGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAg                                                               >  1:583587/1‑140 (MQ=255)
                                                                      tGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAg                                                               >  1:1505290/1‑140 (MQ=255)
                                                                         catcatTATTCCGCATTTTGCCCGATGTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTc                                                            <  2:1355986/140‑1 (MQ=255)
                                                                          atcatTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTa                                                                                 <  2:2997895/118‑1 (MQ=255)
                                                                          atcatTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCa                                                                    <  2:3266247/131‑1 (MQ=255)
                                                                          atcatTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCa                                                                    >  1:3266247/1‑131 (MQ=255)
                                                                          atcatTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTcc                                                           >  2:3377375/1‑140 (MQ=255)
                                                                              ttattCCGCATTTTGCCCGATTGATCGTCGGCAATGATTATCGGTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTGTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTc                                                       <  1:2088660/140‑1 (MQ=255)
                                                                                      gaTTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCAt                                               <  2:1283655/139‑1 (MQ=255)
                                                                                      cATTTTGCCCGATTTATCGTCGGCACTGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCAt                                               >  2:2247416/1‑140 (MQ=255)
                                                                                      cATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCAt                                               <  2:3155213/140‑1 (MQ=255)
                                                                                      cATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCAt                                               >  1:1069608/1‑140 (MQ=255)
                                                                                      cATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCAt                                               >  1:504096/1‑140 (MQ=255)
                                                                                      cATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCAt                                               <  2:2568403/140‑1 (MQ=255)
                                                                                      cATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCGTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCAt                                               >  1:732524/1‑140 (MQ=255)
                                                                                      cATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTGTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCAt                                               >  1:3078577/1‑140 (MQ=255)
                                                                                        ttttGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGa                                             <  2:2043751/140‑1 (MQ=255)
                                                                                        ttgtGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGa                                             <  2:201945/137‑1 (MQ=255)
                                                                                         tttGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCt                                                                      <  1:3667248/114‑1 (MQ=255)
                                                                                                  tttATCGTCGGCACTGAGTATCGTTGGGTTCTGCCGTTTTCCGCTGTCTTGGGTGCGATTTTGCTTGTAGCTGCTGAGATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCcatcat                                   <  2:3479708/140‑1 (MQ=255)
                                                                                                  tttATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCcatcat                                   <  2:1069876/140‑1 (MQ=255)
                                                                                                    tATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCg                                 <  1:2879594/140‑1 (MQ=255)
                                                                                                    tATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCg                                 <  1:3389198/140‑1 (MQ=255)
                                                                                                    tATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCg                                 <  1:1352545/140‑1 (MQ=255)
                                                                                                    tATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCg                                 <  1:3734908/140‑1 (MQ=255)
                                                                                                    tATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCg                                 <  1:1283358/140‑1 (MQ=255)
                                                                                                      tcgtcgGCCATGATTATCGTTGGGTTCTGCCGTTTTCGGGTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTTATCTCGGGGCGCGTTTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGACATGACAGCAATCATCGGt                               <  2:3010056/140‑1 (MQ=255)
                                                                                                      tcgtcgGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGt                               <  1:3491655/140‑1 (MQ=255)
                                                                                                      tcgtcgGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGt                               <  1:1245223/140‑1 (MQ=255)
                                                                                                      tcgtcgGCAATGAATATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGt                               <  2:1651588/140‑1 (MQ=255)
                                                                                                          cgGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGc                           <  1:3682157/140‑1 (MQ=255)
                                                                                                          cgGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGc                           <  1:1861842/140‑1 (MQ=255)
                                                                                                           ggCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGcc                          >  1:2698490/1‑140 (MQ=255)
                                                                                                           ggCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGcc                          <  2:2959564/140‑1 (MQ=255)
                                                                                                           ggCAATGATTATCGTTGGGTTCTGCCGTTTTCAGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGATGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGcc                          >  1:2612595/1‑140 (MQ=255)
                                                                                                             gaaTGATTATCGTGGGGGTCTGCCGTTTTCCGCTGTTTTGGGTGCGAGTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATGATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTAGGCCtg                        <  2:3063355/139‑1 (MQ=255)
                                                                                                             cAATGATTTTCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCTCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATTCCtg                        <  2:896538/140‑1 (MQ=255)
                                                                                                             cAATGATTATCGTTGGGTTCTGCCTTTTTCCGCTGTATTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCtg                        <  1:2375525/140‑1 (MQ=255)
                                                                                                             cAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCtg                        <  1:1767345/140‑1 (MQ=255)
                                                                                                             cAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCtg                        <  2:203245/140‑1 (MQ=255)
                                                                                                             cAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCtg                        <  1:773985/140‑1 (MQ=255)
                                                                                                             cAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCtg                        <  1:2235690/140‑1 (MQ=255)
                                                                                                               aTGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCtgtg                      <  2:3602918/140‑1 (MQ=255)
                                                                                                                     aTCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTtgtg                <  2:1505290/140‑1 (MQ=255)
                                                                                                                     aTCGTTGGGTTCTGCCGTTTTCCGCTGTGTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTtgtg                >  1:1979242/1‑140 (MQ=255)
                                                                                                                       cGTTGGGTTCTGCCGTTTTCCTCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTa              <  2:2309213/140‑1 (MQ=255)
                                                                                                                       cGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTa              >  2:695281/1‑140 (MQ=255)
                                                                                                                       cGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTa              <  2:504096/140‑1 (MQ=255)
                                                                                                                       cGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTa              <  2:3851864/140‑1 (MQ=255)
                                                                                                                           gggTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGcc                                        <  1:1137260/110‑1 (MQ=255)
                                                                                                                           gggTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGcc                                        >  2:1137260/1‑110 (MQ=255)
                                                                                                                            ggTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTACATCg         >  1:230823/1‑140 (MQ=255)
                                                                                                                            ggTTCTGCCGGTTTCCGCTGTTTGGGGTGCGATTTTGCTTGTAGCTGGTGATATCGGGGCGCGTTTTCGCATTATGCCTCAGGGAGGTCGTGTCGGGGGCTTGTCAGCCATCATCGGTCTGGCTGGGTGTGGGTACCTCg         >  2:623022/1‑140 (MQ=255)
                                                                                                                              ttCTGCCATTTTCCGCTGTTTTGGGTGCCATTTTGCTTGTTTGCGCGGATATCGGTGCGCGATATATCATTATGCCTCAGGAAGTTCCTGTCGGGGTCATGACCGCCATCATCGGAATGCCTGTGTTTGTGTACATCGCa       <  2:3826596/140‑1 (MQ=255)
                                                                                                                              ttCTGCCATTTTCCGCTGTTTTGGGTGCCATTTTGCTTGTTTGCGCGGATATCGGTGCGCGATATATCATTATGCCTCAGGAAGTTCCTGTCGGGGTCATGACCGCCATCATCGGAATGCCTGTGTTTGTGTACATCGCa       <  2:1546164/140‑1 (MQ=255)
                                                                                                                                cTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTACATCGCaag     <  2:1190195/140‑1 (MQ=255)
                                                                                                                                cTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTACATCGCaag     <  2:249953/140‑1 (MQ=255)
                                                                                                                                 tGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTACATCGCacga    >  2:2334749/1‑140 (MQ=255)
                                                                                                                                 tGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTACATCGCa       <  1:2990115/137‑1 (MQ=255)
                                                                                                                                   ccGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTACATCGCaagaag  <  1:2468284/140‑1 (MQ=255)
                                                                                                                                   ccGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTACATCGCaagaag  <  2:453566/140‑1 (MQ=255)
                                                                                                                                   ccGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTACATCGCaagaag  <  1:3367067/140‑1 (MQ=255)
                                                                                                                                   ccGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTACATCGCaagaag  <  1:1412192/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                         
TTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGGGTTCTGCCGTTTTCCGCTGTTTTGGGTGCGATTTTGCTTGTAGCTGCTGATATCGGGGCGCGATTTATCATTATGCCTCAGGAAGTTCCTGTCGGGGCCATGACAGCCATCATCGGTATGCCTGTGTTTGTGTACATCGCAAGAAG  >  NZ_CP034943/892065‑892335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: