Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,170,453 A→C 5.5% K312N (AAA→AAC EO946_RS06025 → carbamoyl phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,170,4530AC 5.5% 787.3 / 2.7 314K312N (AAA→AACEO946_RS06025carbamoyl phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base A (117/163);  new base C (2/32);  total (119/195)
Fisher's exact test for biased strand distribution p-value = 1.79e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

GGCTAGTCTGACGATCATCTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCACTGGACTATGTCATCGTCAAATTTCCGC  >  NZ_CP034943/1170315‑1170592
                                                                                                                                          |                                                                                                                                           
ggCTAGTCTGACGATCATCTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAg                                                                                                                                            >  2:3585723/1‑140 (MQ=255)
   tAGTCTGACGATCATCTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGa                                                                                                                                         <  2:3803067/140‑1 (MQ=255)
   tAGTCTGACGATCATCTCAGCACTTGATGTCGTAGGCGGCTGCAAAATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGa                                                                                                                                         <  2:816820/140‑1 (MQ=255)
     gTCTGACGATCATCTCAGCACTTTATGTCGTTGGGGGCTGAAAAATTCAATACGCGGTGGATCCATTCCGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGAcc                                                                                                                                       <  1:3549644/140‑1 (MQ=255)
     gTCTGACGATCATCTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCAATTCAGAAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGAcc                                                                                                                                       <  2:734943/140‑1 (MQ=255)
          aCGATCATCTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCACTATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTa                                                                                                                                  >  1:3591850/1‑140 (MQ=255)
          aCGATCATCTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTa                                                                                                                                  >  1:1460756/1‑140 (MQ=255)
          aCGATCATCTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTa                                                                                                                                  >  1:3769642/1‑140 (MQ=255)
          aCGATCATCTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTa                                                                                                                                  >  2:122494/1‑140 (MQ=255)
            tatcatcTCAGCACTTGATGTCTTAGGCGGCTGCAACATTCATTTCGCGCTGTATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATc                                                                                                                                <  2:2199654/139‑1 (MQ=255)
            gATCATCTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATc                                                                                                                                >  2:3297770/1‑140 (MQ=255)
            gATCATCACAGCACTTGATGTCGTAGGCGGATGCAACATTCAATACGCGCTGCATCAATTCACAACGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAACGCGACCGGTTATc                                                                                                                                <  1:1426296/140‑1 (MQ=255)
               catcTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGa                                                                                                                             >  2:1986415/1‑140 (MQ=255)
                atcTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGAt                                                                                                                            >  1:2881029/1‑140 (MQ=255)
                atcTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGAt                                                                                                                            >  1:1005166/1‑140 (MQ=255)
                         cTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTTAAGTCAATCCGGGTGTAAGCCGTTCATCGGCTCTCGCTTCTAAAGAGACCGGTTATCCGATTGCGAAGAt                                                                                                                   <  2:292772/140‑1 (MQ=255)
                         cTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGAt                                                                                                                   <  1:1798547/140‑1 (MQ=255)
                          ttGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATg                                                                                                                  >  1:3428286/1‑140 (MQ=255)
                            gATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGc                                                                                                                >  1:3107779/1‑140 (MQ=255)
                            gATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGc                                                                                                                >  1:715479/1‑140 (MQ=255)
                               gTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGc                                                                                                             >  2:376227/1‑140 (MQ=255)
                               gTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGc                                                                                                             >  2:1903060/1‑140 (MQ=255)
                                 cGTACGCGGCTGCAACATTCAATTAGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAATCCGGTCATCGGCTCTCGCCTCTAAAGCGACCGGTTATCCGATTGCGAAGATCGCTGCaa                                                                                                           >  2:3162680/1‑140 (MQ=255)
                                   tAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAGGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCaaaa                                                                                                         >  2:2502360/1‑140 (MQ=255)
                                   tAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCaaaa                                                                                                         >  2:1611754/1‑140 (MQ=255)
                                    aGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAAc                                                                                                        >  2:3666289/1‑140 (MQ=255)
                                       gggcTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAACGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTgg                                                                                                     <  1:1663823/139‑1 (MQ=255)
                                       cggcTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTgg                                                                                                     <  1:3872238/140‑1 (MQ=255)
                                         gcTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGAGTGCGAAGATGGCTGCAAAACTGGCa                                                                                                   <  2:459769/140‑1 (MQ=255)
                                          cTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAg                                                                                                  >  2:1013092/1‑140 (MQ=255)
                                          cTGCAACATTCAATTCGCGCGGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAg                                                                                                  <  2:2030395/140‑1 (MQ=255)
                                           tGCAACATTCACGTCGCGCGCGATCCATTCAGCAAGCAATATTATGTCATTGAACTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGt                                                                                                 <  2:2137945/140‑1 (MQ=255)
                                           tGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAACGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGt                                                                                                 <  1:2820672/140‑1 (MQ=255)
                                           tGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGt                                                                                                 <  1:3698555/140‑1 (MQ=255)
                                           tGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGt                                                                                                 <  1:898540/140‑1 (MQ=255)
                                           tGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGt                                                                                                 <  2:1078218/140‑1 (MQ=255)
                                           tGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGt                                                                                                 <  2:904935/140‑1 (MQ=255)
                                           tGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGt                                                                                                 <  1:1903134/140‑1 (MQ=255)
                                           tGCAACATTCAATTCGCGCTGGATCAATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGt                                                                                                 <  1:127789/140‑1 (MQ=255)
                                                 aTTCAATTCGCGCTGTATCCATTCAGAAAGCAATATTATTTCATTGAAGTCAATCCGCTTGTAAGCCGGTCATCGGCTCTCGCGTCTAACGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGata                                                                                           <  1:2495155/140‑1 (MQ=255)
                                                 aTTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAACGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGata                                                                                           <  1:1531613/140‑1 (MQ=255)
                                                 aTTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGata                                                                                           <  2:2801256/140‑1 (MQ=255)
                                                 aTTCAATTCGCGCTGGATCCATTCAGCAAGAAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGata                                                                                           <  2:3849014/140‑1 (MQ=255)
                                                 aTTAAATTCGCGCTGGATCCATTCAGCAAGAAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAACGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGata                                                                                           <  1:2298100/140‑1 (MQ=255)
                                                   tCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGAtata                                                                                         >  1:2948539/1‑140 (MQ=255)
                                                    cAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATAc                                                                                        <  1:1382134/140‑1 (MQ=255)
                                                    cAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATAc                                                                                        <  1:224798/140‑1 (MQ=255)
                                                       ttCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCt                                                                                     >  2:1770415/1‑140 (MQ=255)
                                                         cgcgCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTtg                                                                                   <  2:191476/140‑1 (MQ=255)
                                                         cgcgCTGGATCCATTCAGCAACCAATATTATGTCATTTACGACAATCCGCGTGTAAGCCGGTAATCGGCTCTCGCGAATAACGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTtg                                                                                   <  1:2650395/140‑1 (MQ=255)
                                                           cgcTGGATCCATTCAGCAAGCAATATTATTTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTtgat                                                                                 <  2:1955975/140‑1 (MQ=255)
                                                           cgcTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTtgat                                                                                 >  2:396827/1‑140 (MQ=255)
                                                            gcTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTtgatg                                                                                >  2:3057896/1‑140 (MQ=255)
                                                                           caagcaaTATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGc                                                                 >  2:2744264/1‑140 (MQ=255)
                                                                                   attatGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGt                                                         <  1:1716634/140‑1 (MQ=255)
                                                                                   attatGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGt                                                         <  1:2341235/140‑1 (MQ=255)
                                                                                   attatGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGt                                                         >  1:503006/1‑140 (MQ=255)
                                                                                   attatGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGt                                                         >  1:2061754/1‑140 (MQ=255)
                                                                                         tCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACt                                                   >  1:772338/1‑140 (MQ=255)
                                                                                          cATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTcac                                                             >  1:614948/1‑129 (MQ=255)
                                                                                          cATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTcac                                                             >  1:2913773/1‑129 (MQ=255)
                                                                                          cATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTcac                                                             <  2:614948/129‑1 (MQ=255)
                                                                                            ttGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTAc                                                >  1:2168580/1‑140 (MQ=255)
                                                                                            ttGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTAc                                                >  2:284100/1‑140 (MQ=255)
                                                                                             tGAAGTCAATCCGCGTGTAAGCCGTTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACg                                               <  2:1426420/140‑1 (MQ=255)
                                                                                                 gTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAg                                           >  2:2938696/1‑140 (MQ=255)
                                                                                                         gcttttAAGCCGGTCATCGGCTCACGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAAc                                   <  2:2371383/135‑1 (MQ=255)
                                                                                                         gcgTGTAAGCCGTTCATCGGCTCTCGCGTCTAACGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAAc                                   <  1:122694/140‑1 (MQ=255)
                                                                                                         gcgTGTAAGCCGGTCATCGGCTCTCGCGTCTAACGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAAc                                   <  1:1576892/140‑1 (MQ=255)
                                                                                                         gcgTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAAc                                   <  1:1937725/140‑1 (MQ=255)
                                                                                                         gcgTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAAc                                   <  1:982248/140‑1 (MQ=255)
                                                                                                         gcgTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAAc                                   <  1:2772685/140‑1 (MQ=255)
                                                                                                         gcgTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAAc                                   <  2:3019123/140‑1 (MQ=255)
                                                                                                         gcgTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCACTCACAGGTTCGACTTACGCAAGCTTTGAAc                                   <  2:342521/140‑1 (MQ=255)
                                                                                                         gcgTGTAAGCCGGTCATCGGCTCTCGCGTATAAAGCTACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAAc                                   <  1:2434448/140‑1 (MQ=255)
                                                                                                              ttaGCCGTTCATGGGCTCTCGCGTCTAACGCGCCCGGTTATCCGATTGCTAAGATGGCTGCAAAACTGGCAGTCGTATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGAGTTACGCAAGCTTTGAACCTGCa                              <  2:3539667/138‑1 (MQ=255)
                                                                                                              tatgCCGGTCATCGGCTCTCGCTTATAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  1:562874/137‑1 (MQ=255)
                                                                                                              tacgCCGGTCATCGGCTCTCGCGTCTAACGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  2:1562674/137‑1 (MQ=255)
                                                                                                              tAAGCCGGTCATCGGCTCTCGCGTCTAACGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAATCTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  2:3078966/140‑1 (MQ=255)
                                                                                                              tAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGTTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  2:2591873/140‑1 (MQ=255)
                                                                                                              tAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  1:3297770/140‑1 (MQ=255)
                                                                                                              tAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  1:1968419/140‑1 (MQ=255)
                                                                                                              tAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  1:25797/140‑1 (MQ=255)
                                                                                                              tAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  1:2864550/140‑1 (MQ=255)
                                                                                                              tAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  1:3057896/140‑1 (MQ=255)
                                                                                                              tAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  1:3080592/140‑1 (MQ=255)
                                                                                                              tAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTGATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  2:535085/140‑1 (MQ=255)
                                                                                                              tAAGCCGGTCATCGGCTCGCGCGTCTAACGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  2:1170844/140‑1 (MQ=255)
                                                                                                              tAAGCCGGTCATCGGCTATCTCGACTAAAGGGACCGGTTATCCGATTTCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCGTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTAAGCAAGCTTTGAACCTGCa                              <  1:2743127/140‑1 (MQ=255)
                                                                                                              tAAGCCGGTAATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACACTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCa                              <  2:1658414/140‑1 (MQ=255)
                                                                                                               aaGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCAc                             <  2:2877892/140‑1 (MQ=255)
                                                                                                                    ggTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCACTGGAc                        >  1:798592/1‑140 (MQ=255)
                                                                                                                     gTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCtt                                        <  2:2887954/123‑1 (MQ=255)
                                                                                                                            gCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCACTGGACTATGTCAt                <  1:1179939/140‑1 (MQ=255)
                                                                                                                                       tAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCACTGGACTATGTCATCGTCAAATTTc     >  2:3708175/1‑140 (MQ=255)
                                                                                                                                       tAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCACTGGACTATGTCATCGTCAAATTTc     >  2:2621693/1‑140 (MQ=255)
                                                                                                                                       tAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCACTGGACTATGTCATCGTCAAATTTc     >  1:3000221/1‑140 (MQ=255)
                                                                                                                                       tAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCACTGGACTATGTCATCGTCAAATTTc     >  1:104629/1‑140 (MQ=255)
                                                                                                                                       tAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACATGCACTGGACTATGTCATCGTCAAATTTc     >  1:1084147/1‑140 (MQ=255)
                                                                                                                                          aGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCACTGGACTATGTCATCGTCAAATTTCcgc  >  1:3160664/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GGCTAGTCTGACGATCATCTCAGCACTTGATGTCGTAGGCGGCTGCAACATTCAATTCGCGCTGGATCCATTCAGCAAGCAATATTATGTCATTGAAGTCAATCCGCGTGTAAGCCGGTCATCGGCTCTCGCGTCTAAAGCGACCGGTTATCCGATTGCGAAGATGGCTGCAAAACTGGCAGTCGGATATACCCTTGATGAGCTGAAAAATCCGCTCACAGGTTCGACTTACGCAAGCTTTGAACCTGCACTGGACTATGTCATCGTCAAATTTCCGC  >  NZ_CP034943/1170315‑1170592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: