Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,215,179 A→C 5.7% K81N (AAA→AAC thiF → thiazole biosynthesis adenylyltransferase ThiF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,215,1790AC 5.7% 754.7 / 3.6 297K81N (AAA→AACthiFthiazole biosynthesis adenylyltransferase ThiF
Reads supporting (aligned to +/- strand):  ref base A (161/105);  new base C (2/29);  total (163/134)
Fisher's exact test for biased strand distribution p-value = 4.65e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

GGAACAGCGAGTGCTGAAATGCTGGTCAGAGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATCATTGTGGATGCGGCTGA  >  NZ_CP034943/1215042‑1215310
                                                                                                                                         |                                                                                                                                   
ggAACAGCGTGTGCTGAAATGCTGGTAAGAGCGGGAGTTGGCTCTGTGAAATTTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGc                                                                                                                                   <  2:1477745/140‑1 (MQ=255)
     agggAGTGCTTAAATGGTGGTCTTAGCGGGAGTTGGCTTTTTGAAAATTGCCTACATAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGAAGCTGTATACAGAAGATGATGTAAGAAAATAAATACCCAACGCGGCcg                                                                                                                              <  1:2748497/137‑1 (MQ=255)
     aGCGAGTGCTGAAATGCTGGTCAGCGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAAGGGAGCAACCTGCAGCGCCAGCAGCTTTATACAGAAGATGATGTCACAAAAGAAATACCGAAAGCGGCcg                                                                                                                              <  1:191351/140‑1 (MQ=255)
     aGCGAGTGCTGAAATGCTGGTCAGAGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGTTGTCAGAAAACAAATACCGAAAGCGGCcg                                                                                                                              <  2:3425721/140‑1 (MQ=255)
       cGAGTGCTGAAATGCTGGTCAGAGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCcgcg                                                                                                                            >  1:1211279/1‑140 (MQ=255)
       cGAGTGCTGAAATGCTGGTCAGAGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCcgcg                                                                                                                            >  1:1717484/1‑140 (MQ=255)
        gAGTGCTGAAATGCTGGTCAGAGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCgg                                                                                                                           >  2:1058077/1‑140 (MQ=255)
        gAGTGCTGAAATGCTGGTCAGAGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCgg                                                                                                                           >  2:1217615/1‑140 (MQ=255)
          gTGCTGAACTGCTGGTCAGAGCGGGCGTGGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCACCCTGCCGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAACAAATCCCGAACGCGGCCGCGGCt                                                                                                                         <  1:1418040/140‑1 (MQ=255)
            gCTGAAATGCTGGTCAGAGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGa                                                                                                                       >  2:1187493/1‑140 (MQ=255)
               gAAATGCTGGTCAGAGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCa                                                                                                                    >  2:2728607/1‑140 (MQ=255)
               gAAATGCTGGTCAGAGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCa                                                                                                                    >  2:1384826/1‑140 (MQ=255)
                 aaTGCTGGTCAGAGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATc                                                                                                                  >  2:1920421/1‑140 (MQ=255)
                         tCAGAGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATGACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAACTACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGt                                                                                                          <  2:1164621/140‑1 (MQ=255)
                             agCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCAGCGTCTCCGTTCGa                                                                                                      >  1:2455053/1‑140 (MQ=255)
                                  gAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAAc                                                                                                 >  1:1341797/1‑140 (MQ=255)
                                     ttGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAACGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGc                                                                                              <  2:2005188/140‑1 (MQ=255)
                                       ggCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGcga                                                                                            >  1:2889230/1‑140 (MQ=255)
                                       ggCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGcga                                                                                            >  1:2010327/1‑140 (MQ=255)
                                       ggCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGcga                                                                                            >  2:2590414/1‑140 (MQ=255)
                                       ggCTCGGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTCTACAGAAGATGATGGCAGAACAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCGCCGTTCGATTAACTGcga                                                                                            >  2:619993/1‑140 (MQ=255)
                                        gCTCTGTGAAAATTGCCGACAGAGATTCCGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTAAACACAAGATGATGACAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGcgat                                                                                           <  1:1489138/140‑1 (MQ=255)
                                        gCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGcgat                                                                                           <  1:2677449/140‑1 (MQ=255)
                                            tgtgAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGcgatgtcg                                                                                       >  2:3345637/1‑140 (MQ=255)
                                            tgtgAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGcgatgtcg                                                                                       >  1:3614380/1‑140 (MQ=255)
                                                         gACAGATATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCt                                                                          <  1:2248824/140‑1 (MQ=255)
                                                         gACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCt                                                                          <  2:2464239/140‑1 (MQ=255)
                                                         gACAGAGATTACGTTGAAGGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAACGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCt                                                                          <  1:1403756/140‑1 (MQ=255)
                                                             gagaTTTCGGTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATCCAGAAGCTGATGTCAGAAAAGAAATACCGAAAGCGGGCGGGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGtt                                                                      <  1:973678/140‑1 (MQ=255)
                                                             gagaTTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATCCCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGtt                                                                      <  1:1869395/140‑1 (MQ=255)
                                                             cagaTAACGTTCATTGGAGCAACGTGCAGCGCCAGCAGCTGTATCCAGACGATGAGGTCAGAAACGAAATACCGAAAGCGGCCGCGGCTGAGCAGCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGtt                                                                      <  1:3357806/139‑1 (MQ=255)
                                                                       tGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAATAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGa                                                            <  1:1164121/140‑1 (MQ=255)
                                                                        gAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGAc                                                           >  2:840250/1‑140 (MQ=255)
                                                                         aaTGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGACAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGGTATGGATGTGAct                                                          >  1:1000841/1‑139 (MQ=255)
                                                                         aaTGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACa                                                          >  1:558679/1‑140 (MQ=255)
                                                                              aGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGa                                                     >  1:1196454/1‑140 (MQ=255)
                                                                              aGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGa                                                     >  2:3743065/1‑140 (MQ=255)
                                                                              aGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGa                                                     >  2:1895220/1‑140 (MQ=255)
                                                                                   ccTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAata                                                >  1:2313893/1‑140 (MQ=255)
                                                                                   ccTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAata                                                >  1:3219910/1‑140 (MQ=255)
                                                                                        aGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGt                                                                                                          >  2:2811662/1‑77 (MQ=255)
                                                                                        aGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCt                                                                          <  2:3769558/109‑1 (MQ=255)
                                                                                        aGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTc                                           >  1:514644/1‑140 (MQ=255)
                                                                                        aGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTc                                           >  1:2540149/1‑140 (MQ=255)
                                                                                        aGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTGCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTc                                           >  1:3792406/1‑140 (MQ=255)
                                                                                          cgcCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAACGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGa                                         <  1:1259886/140‑1 (MQ=255)
                                                                                           gcCAGCAGGTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  1:154860/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTTTATACATAAGATTATGTCATAAAAGAAATACCGACCGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  1:266009/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTTTATACAGAAGATTATGTCACAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  1:249636/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTTGATCCAGCAGTTGCTGACAGAACCCAAATCCCCACCGCGGCCGCGGCTGAGCATCGTCTCCGTTCGAGTAACAGCGATGTCGATGTGACTGGCCTTGTTAGGGATGTGCCAGCGGAAAATATCTTCGAg                                        <  1:2246892/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTGTCTCCTGAAGATGATGTCAGAAAAGAAAGACCGACCGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  1:2618172/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAACGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  1:1125132/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAACGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGGGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  2:1529490/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATTGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  1:1058077/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  2:68417/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  1:3748775/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  1:1673876/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  1:3689300/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  1:3227563/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  1:3112778/140‑1 (MQ=255)
                                                                                           gcCAGCAGCTGTATACAGAAGATGATGACAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  2:2517543/140‑1 (MQ=255)
                                                                                           gcCAGAAGCTGTATACAGAAGATTTTCTCATAAAAGAAATCCCGAACGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        <  1:3310470/140‑1 (MQ=255)
                                                                                             cagcagCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCt                                      >  2:3054508/1‑140 (MQ=255)
                                                                                             cagcagCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCt                                      >  2:639593/1‑140 (MQ=255)
                                                                                              agcagcTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTg                                     >  1:47101/1‑140 (MQ=255)
                                                                                                 agcTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGAtt                                  >  2:3851895/1‑140 (MQ=255)
                                                                                                      tataCAGAAGATGATGTCAGAAAAGAACTACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTACGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAggg                             <  2:1661896/140‑1 (MQ=255)
                                                                                                         aCAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGgcgc                          <  2:3600798/140‑1 (MQ=255)
                                                                                                          cAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCt                         <  2:2325095/140‑1 (MQ=255)
                                                                                                          cAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCt                         <  2:2121311/140‑1 (MQ=255)
                                                                                                           agaagaTGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCtt                        >  2:444505/1‑140 (MQ=255)
                                                                                                           agaagaTGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCtt                        <  2:514644/140‑1 (MQ=255)
                                                                                                            gaagaTGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTc                       >  1:1774801/1‑140 (MQ=255)
                                                                                                             aagaTGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGcgat                                                                                           >  1:184879/1‑71 (MQ=255)
                                                                                                             aagaTGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGACAATATCTTCGAGCTGATTAAGGGCGCTTCg                      >  1:1330098/1‑140 (MQ=255)
                                                                                                             aagaTGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAg                                        >  1:294264/1‑122 (MQ=255)
                                                                                                             aagaTGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCg                      >  2:1780513/1‑140 (MQ=255)
                                                                                                             aagaTGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCg                      >  1:3532861/1‑140 (MQ=255)
                                                                                                             aagaTGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCg                      >  1:2368293/1‑140 (MQ=255)
                                                                                                             aagaTGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCg                      >  1:3154462/1‑140 (MQ=255)
                                                                                                             aagaTGATGTCAGAAAAGAAATACCGAAACCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCg                      >  1:713200/1‑140 (MQ=255)
                                                                                                               gatgatGTCAGAAAAGAAATACCGCAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGAt                    <  1:2203986/140‑1 (MQ=255)
                                                                                                               gatgatGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGAt                    >  1:2122965/1‑140 (MQ=255)
                                                                                                                atgatgTCAGAAAAGAAATACCGACAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATc                   >  2:1835306/1‑140 (MQ=255)
                                                                                                                atgatgTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTTTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGAGGTGACAGCGGAAAATATCTTCGAGCTGATGAAGGGCGCTTCGATc                   >  2:3701939/1‑140 (MQ=255)
                                                                                                                atgatgTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGt                                                                                                          <  2:2388952/53‑1 (MQ=255)
                                                                                                                atgatgTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGt                                                                                                          >  1:2388952/1‑53 (MQ=255)
                                                                                                                atgatgTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGc                                                                            >  1:1215517/1‑83 (MQ=255)
                                                                                                                atgatgTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATc                   >  2:169996/1‑140 (MQ=255)
                                                                                                                atgatgTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATc                   >  1:1063417/1‑140 (MQ=255)
                                                                                                                atgatgTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATc                   >  1:2994518/1‑140 (MQ=255)
                                                                                                                atgatgTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATc                   >  1:785656/1‑140 (MQ=255)
                                                                                                                  gatgTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATCAt                 >  2:940440/1‑140 (MQ=255)
                                                                                                                    tgTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATCATtg               >  1:2828792/1‑140 (MQ=255)
                                                                                                                       cAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGcg                                                                                             <  1:867188/59‑1 (MQ=255)
                                                                                                                       cAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATCATTGTgg            >  2:2032266/1‑140 (MQ=255)
                                                                                                                         gaaaagaaaTACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATCATTGTGGAt          >  2:2993671/1‑140 (MQ=255)
                                                                                                                         gaaaagaaaTACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATCATGGTGGAt          >  2:1721146/1‑140 (MQ=255)
                                                                                                                         gaaaagaaaTACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGAGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATCAGTGTGGAt          >  2:3241017/1‑140 (MQ=255)
                                                                                                                                 aTACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATCATTGTGGATGCGGCTGa  <  1:3054508/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                   
GGAACAGCGAGTGCTGAAATGCTGGTCAGAGCGGGAGTTGGCTCTGTGAAAATTGCCGACAGAGATTACGTTGAATGGAGCAACCTGCAGCGCCAGCAGCTGTATACAGAAGATGATGTCAGAAAAGAAATACCGAAAGCGGCCGCGGCTGAGCATCGTCTCCGTTCGATTAACAGCGATGTCGATGTGACTGGCCTTGTTATGGATGTGACAGCGGAAAATATCTTCGAGCTGATTAAGGGCGCTTCGATCATTGTGGATGCGGCTGA  >  NZ_CP034943/1215042‑1215310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: