Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,243,010 T→A 5.0% V650E (GTG→GAG)  manP → PTS mannose transporter subunit IIABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,243,0100TA 5.0% 889.9 / 23.9 259V650E (GTG→GAG) manPPTS mannose transporter subunit IIABC
Reads supporting (aligned to +/- strand):  ref base T (120/124);  new base A (8/5);  total (129/130)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

GTACCGAAAGAGAGCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCTTTT  >  NZ_CP034943/1242871‑1243149
                                                                                                                                           |                                                                                                                                           
  aCCGAAAGAGAGCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGCAGCATACAAGCTGCTGGATGAAATCgtgt                                                                                                                                           <  1:2140639/140‑1 (MQ=255)
   ccGAAAGAGAGCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTa                                                                                                                                          <  1:2823028/140‑1 (MQ=255)
   ccGAAAGAGAGCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTa                                                                                                                                          <  2:310690/140‑1 (MQ=255)
    cGAAAGAGAGCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAg                                                                                                                                         >  2:1008232/1‑140 (MQ=255)
    cGAAAGAGAGCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAg                                                                                                                                         >  2:1726128/1‑140 (MQ=255)
    cGAAAGAGAGCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAg                                                                                                                                         >  2:1788957/1‑140 (MQ=255)
      aaaGAGAGCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTg                                                                                                                                       >  1:3069174/1‑140 (MQ=255)
      aaaGAGAGCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTg                                                                                                                                       >  1:1508207/1‑140 (MQ=255)
           gagCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCgggg                                                                                                                                  >  2:2530892/1‑140 (MQ=255)
           gagCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCgggg                                                                                                                                  >  2:3194011/1‑140 (MQ=255)
           gagCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATCATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCgggg                                                                                                                                  >  2:1353512/1‑140 (MQ=255)
             gCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCgggggg                                                                                                                                <  2:92296/140‑1 (MQ=255)
             gCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCgggggg                                                                                                                                >  2:1965954/1‑140 (MQ=255)
             gCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCgggggg                                                                                                                                <  2:3334796/140‑1 (MQ=255)
              cGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCGATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGa                                                                                                                               >  2:2723694/1‑140 (MQ=255)
                     aaCCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACACAAGAAGCAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCAt                                                                                                                        <  1:2626773/140‑1 (MQ=255)
                           cATCTCAAAATACTGCAGATGCTATCTCGAAAGCTAATGGATGACAATTATAGGGAGCGGCTGCTCTCCGTGCAAACAACAGAAGAAGCATACAAGCTGCTAGAAGAAATCGAATAAAGCGGGGGATTTTCATgacgact                                                                                                                 >  2:1216218/1‑139 (MQ=255)
                           cATCTCAAAATACTGCAGATGCTATCTCGAAAGCTAATGGATGACAATTATAGGGAGCGGCTGCTCTCCGTGCAAACAACAGAAGAAGCATACAAGCTGCTAGAAGAAATCGAATAAAGCGGGGGATTTTCATgacgact                                                                                                                 >  2:3847221/1‑139 (MQ=255)
                             tCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCAt                                                                                                                        <  1:2266543/132‑1 (MQ=255)
                             tCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAg                                                                                                                >  1:1007745/1‑140 (MQ=255)
                               tGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCTGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGAGGACAGaa                                                                                                              >  2:2079457/1‑140 (MQ=255)
                               tGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGaa                                                                                                              >  2:3476081/1‑140 (MQ=255)
                                   aaTATTGCAGATGTGATCCCGAAAACGAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGAGGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGt                                                                                                          <  2:325144/140‑1 (MQ=255)
                                     tatTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTa                                                                                                        <  1:2894350/140‑1 (MQ=255)
                                     tatTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTa                                                                                                        <  1:3048684/140‑1 (MQ=255)
                                     tatTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGCGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTa                                                                                                        <  1:945492/140‑1 (MQ=255)
                                        tGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCgtgt                                                                                                                                           <  1:1926681/102‑1 (MQ=255)
                                        tGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCTTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTAttt                                                                                                     >  2:616409/1‑140 (MQ=255)
                                        tGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCAt                                                                                                                        >  1:891323/1‑121 (MQ=255)
                                        tGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTAttt                                                                                                     >  1:3509848/1‑140 (MQ=255)
                                        tGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTAttt                                                                                                     >  1:653153/1‑140 (MQ=255)
                                         ggaGATGTTATCCCGAAAACTTATGGATGATAGGTATAGGACGCAGCTTCTCTCCGTGCACACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTCGTGCGGGGGGCTATTCATTAGGAGAGAACCGTTAtttt                                                                                                    >  1:2970330/3‑140 (MQ=255)
                                         gCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGACGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTAtttt                                                                                                    >  2:1422049/1‑140 (MQ=255)
                                         gCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTAtttt                                                                                                    >  1:1226725/1‑140 (MQ=255)
                                           aGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTc                                                                                                  >  1:1083768/1‑140 (MQ=255)
                                                ttATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGcc                                                                                             <  2:599185/140‑1 (MQ=255)
                                                  aTCTCGAAAGCTAATGGATGACAATTATAGGGAGCGGCTGCTCTCCGTGCAAACAACAGAAGAAGCATACAAGCTGCTAGAAGAAATCGAATAAAGCGGGGGATTTTCATGACGACTGAACCGTTATTTTTCAAGCCTGt                                                                                          >  2:126185/1‑140 (MQ=255)
                                                  aTCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTg                                                                                           >  2:538417/1‑140 (MQ=255)
                                                        aaaaCTAATGGATGATAGCTATAGGAAGCAGCTGCACTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCa                                                                                     >  2:3681175/1‑140 (MQ=255)
                                                             tAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAagag                                                                                <  2:2982280/140‑1 (MQ=255)
                                                             tAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAagag                                                                                <  1:781643/140‑1 (MQ=255)
                                                             tAATCGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAATCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAagag                                                                                <  2:2221137/140‑1 (MQ=255)
                                                                   atgagAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAACGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGAtt                                                                          <  2:2500423/140‑1 (MQ=255)
                                                                     gatAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTg                                                                        >  1:3798205/1‑140 (MQ=255)
                                                                     gatAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTg                                                                        >  1:1821652/1‑140 (MQ=255)
                                                                       tAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCg                                                                                                                                     >  2:3056204/1‑77 (MQ=255)
                                                                       tAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTggg                                                                      >  2:791713/1‑140 (MQ=255)
                                                                       tAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTggg                                                                      >  2:3388342/1‑140 (MQ=255)
                                                                              aGGAAGCAGCTGCTCTCCGTTCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTAc                                                               <  2:449178/140‑1 (MQ=255)
                                                                              aGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTAc                                                               <  2:3305/140‑1 (MQ=255)
                                                                              aGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAAGCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTAc                                                               >  2:2956277/1‑140 (MQ=255)
                                                                                 aaGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGAGTTGGGGCGGTACCGc                                                            >  2:3653901/1‑140 (MQ=255)
                                                                                  agcagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCt                                                           >  1:333166/1‑140 (MQ=255)
                                                                                   gcagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTg                                                                                                                                       <  2:216982/63‑1 (MQ=255)
                                                                                    cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                         >  2:850569/1‑140 (MQ=255)
                                                                                    cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                         >  2:2439035/1‑140 (MQ=255)
                                                                                    cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                         >  2:1219760/1‑140 (MQ=255)
                                                                                    cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                         >  2:1615/1‑140 (MQ=255)
                                                                                    cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                         >  2:749817/1‑140 (MQ=255)
                                                                                    cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                         >  1:1015004/1‑140 (MQ=255)
                                                                                     agcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTa                                                        >  2:1877186/1‑140 (MQ=255)
                                                                                      gctgctCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTAt                                                       <  1:1424870/140‑1 (MQ=255)
                                                                                          ctctCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTAc                                                               >  1:2981556/1‑128 (MQ=255)
                                                                                            ctcCGTGCAAACAAAATAAGACGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGAGTTGGGGCGGTACCGCTTTATCGGCtt                                                 <  1:1353512/140‑1 (MQ=255)
                                                                                              ccGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTc                                               <  2:1508207/140‑1 (MQ=255)
                                                                                                gTGCACACAAAAGAAGAAGCATACACGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGACAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCgg                                             <  1:1331048/140‑1 (MQ=255)
                                                                                                gTGCAAACAAAAGAAGAAGCATACACGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCgg                                             <  1:170869/140‑1 (MQ=255)
                                                                                                gTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCgg                                             <  1:2715766/140‑1 (MQ=255)
                                                                                                gTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCgg                                             <  1:808342/140‑1 (MQ=255)
                                                                                                gTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCgg                                             <  1:3622272/140‑1 (MQ=255)
                                                                                                    aaacaaaAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGTGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTAt                                         <  2:1953763/140‑1 (MQ=255)
                                                                                                    aaacaaaAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTAt                                         <  2:1989826/140‑1 (MQ=255)
                                                                                                        aaaaGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATcaca                                     <  2:1385645/140‑1 (MQ=255)
                                                                                                        aaaaGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATcaca                                     <  2:1799423/140‑1 (MQ=255)
                                                                                                            gaagaagCATACAAGCTGCTAGAAGAAATCGAATAAAGCGGGGGATTTTCATGACGACTGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAAAGAATTTGGGGCGGGACCGCTTTAGCTGATTTTGGCTATACCATTCCg                                <  1:1216218/140‑1 (MQ=255)
                                                                                                            gaagaagCATACAAGCTGCTAGAAGAAATCGAATAAAGCGGGGGATTTTCATGACGACTGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAAAGAATTTGGGGCGGGACCGCTTTAGCTGATTTTGGCTATACCATTCCg                                <  1:3847221/140‑1 (MQ=255)
                                                                                                            gaagaagCATACAAGCTGCTAGAAGAAATCGAATAAAGCGGGGGATTTTCATGACGACTGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAAAGAATTTGGGGCGGGACCGCTTTAGCTGATTTTGGCTATACCATTCCg                                <  1:2505413/140‑1 (MQ=255)
                                                                                                                  gCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAga                           <  1:3451992/140‑1 (MQ=255)
                                                                                                                  gCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAga                           <  1:1281620/140‑1 (MQ=255)
                                                                                                                       ccaGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAgaacgaa                      <  2:1465277/138‑1 (MQ=255)
                                                                                                                       cAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAGGAGAGGAGGTGGGGCGGTCCCGCTTTATCGGCTTTCGGCTATCCCATTCCGTGAgtccgac                      >  1:2235922/1‑134 (MQ=255)
                                                                                                                       cAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAgaacgaa                      <  2:360176/140‑1 (MQ=255)
                                                                                                                          gctgctGGATGAAATGGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTACGTCAGAACGAACAg                   >  1:43227/1‑140 (MQ=255)
                                                                                                                          gctgctGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAg                   >  1:539044/1‑140 (MQ=255)
                                                                                                                          gctgctGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAg                   >  1:339726/1‑140 (MQ=255)
                                                                                                                            tgctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCAGCTTCCGTCAGAACGAACAggg                 <  1:1582628/140‑1 (MQ=255)
                                                                                                                            tgctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAggg                 <  1:1334924/140‑1 (MQ=255)
                                                                                                                            tgctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAggg                 <  2:2587852/140‑1 (MQ=255)
                                                                                                                            tgctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAggg                 <  1:3181017/140‑1 (MQ=255)
                                                                                                                             gctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGAtt                                                                          >  2:881654/1‑82 (MQ=255)
                                                                                                                             gctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAgggg                >  1:2054491/1‑140 (MQ=255)
                                                                                                                             gctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAgggg                >  2:3529704/1‑140 (MQ=255)
                                                                                                                                 gATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGCGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTg            <  2:286286/140‑1 (MQ=255)
                                                                                                                                  aTGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGc           <  1:3114836/140‑1 (MQ=255)
                                                                                                                                    gAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTg         >  1:13101/1‑140 (MQ=255)
                                                                                                                                    gAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTg         <  1:24315/140‑1 (MQ=255)
                                                                                                                                        tCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCt     >  2:1167308/1‑140 (MQ=255)
                                                                                                                                           tgtAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAATGAGAGGATTTGGGGCTGTACCGATTTATCGGCTTTCGGCTATCACATTCCGTCAGAACTAACAGGGGAGTGCTGTGCtttt  >  2:2332408/1‑140 (MQ=255)
                                                                                                                                           tgtAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCtttt  >  2:279568/1‑140 (MQ=255)
                                                                                                                                           tgtAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCAGTAAGAACGAACAGGGGCGTGCTGGGCtttt  >  2:2269312/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GTACCGAAAGAGAGCGAAGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCTTTT  >  NZ_CP034943/1242871‑1243149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: