Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,243,026 A→T 5.6% intergenic (+12/‑4) manP → / → manA PTS mannose transporter subunit IIABC/mannose‑6‑phosphate isomerase, class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,243,0260AT 5.6% 818.8 / 27.4 242intergenic (+12/‑4)manP/manAPTS mannose transporter subunit IIABC/mannose‑6‑phosphate isomerase, class I
Reads supporting (aligned to +/- strand):  ref base A (115/113);  new base T (7/7);  total (122/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

AGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCTTTTGCTGCTCATAAAAAC  >  NZ_CP034943/1242888‑1243164
                                                                                                                                          |                                                                                                                                          
aGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAACCAAAAGAAGAAGCCTACAAGCTGCTGGCTGACATCGGGGCGTGCGGGGGGatat                                                                                                                                           >  1:3225988/1‑140 (MQ=255)
aGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGatat                                                                                                                                           >  1:3347938/1‑140 (MQ=255)
aGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGCGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGatat                                                                                                                                           >  1:1842086/1‑140 (MQ=255)
    aaCCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCAt                                                                                                                                       <  1:1014271/140‑1 (MQ=255)
    aaCCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCAt                                                                                                                                       <  2:350923/140‑1 (MQ=255)
    aaCCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCAt                                                                                                                                       <  2:622080/140‑1 (MQ=255)
    aaCCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCAt                                                                                                                                       <  2:508338/140‑1 (MQ=255)
          cATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATgacgac                                                                                                                                 >  2:694665/1‑140 (MQ=255)
          cATCTCAAAATACTGCAGATGCTATCTCGAAAGCTAATGGATGACAATTATAGGGAGCGGCTGCTCTCCGTGCAAACAACAGAAGAAGCATACAAGCTGCTAGAAGAAATCGAATAAAGCGGGGGATTTTCATgacgact                                                                                                                                >  2:1216218/1‑139 (MQ=255)
          cATCTCAAAATACTGCAGATGCTATCTCGAAAGCTAATGGATGACAATTATAGGGAGCGGCTGCTCTCCGTGCAAACAACAGAAGAAGCATACAAGCTGCTAGAAGAAATCGAATAAAGCGGGGGATTTTCATgacgact                                                                                                                                >  2:2505413/1‑139 (MQ=255)
            tCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAg                                                                                                                               >  1:1007745/1‑140 (MQ=255)
                  aaTATTGCAGATGTGATCCCGAAAACGAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGAGGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGt                                                                                                                         <  2:325144/140‑1 (MQ=255)
                    tatTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTa                                                                                                                       >  2:3605101/1‑140 (MQ=255)
                       tGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCACACAAAAGAAGAAGCATACACGCTGCTGGATGAACTCGTGTAGTGCGGGGGGATATACATGACGAGAGAACCGTTAttt                                                                                                                    >  2:3310483/1‑140 (MQ=255)
                       tGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTAttt                                                                                                                    >  1:653153/1‑140 (MQ=255)
                       tGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTAttt                                                                                                                    >  1:3509848/1‑140 (MQ=255)
                       tGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTAATGACTACAGAACCGTTAttt                                                                                                                    >  2:3370363/1‑140 (MQ=255)
                        ggaGATGTTATCCCGAAAACTTATGGATGATAGGTATAGGACGCAGCTTCTCTCCGTGCACACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTCGTGCGGGGGGCTATTCATTAGGAGAGAACCGTTAtttt                                                                                                                   >  1:2970330/3‑140 (MQ=255)
                        gCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTAtttt                                                                                                                   >  1:1226725/1‑140 (MQ=255)
                          aGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTc                                                                                                                 >  1:3792520/1‑140 (MQ=255)
                          aGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTc                                                                                                                 >  2:1424870/1‑140 (MQ=255)
                          aGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTc                                                                                                                 >  1:1083768/1‑140 (MQ=255)
                               ttATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGcc                                                                                                            <  2:2691069/140‑1 (MQ=255)
                                 aTCTCGAAAGCTAATGGATGACAATTATAGGGAGCGGCTGCTCTCCGTGCAAACAACAGAAGAAGCATACAAGCTGCTAGAAGAAATCGAATAAAGCGGGGGATTTTCATGACGACTGAACCGTTATTTTTCAAGCCTGt                                                                                                         >  2:126185/1‑140 (MQ=255)
                                 aTCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTg                                                                                                          >  1:874109/1‑140 (MQ=255)
                                       aaaaCTAATGGATGATAGCTATAGGAAGCAGCTGCACTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCa                                                                                                    >  2:3681175/1‑140 (MQ=255)
                                            tactGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAagag                                                                                               <  1:318065/137‑1 (MQ=255)
                                            tAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAagag                                                                                               <  1:781643/140‑1 (MQ=255)
                                            tAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAagag                                                                                               <  1:2394091/140‑1 (MQ=255)
                                            tAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAagag                                                                                               <  1:1264719/140‑1 (MQ=255)
                                            tAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAagag                                                                                               <  2:2982280/140‑1 (MQ=255)
                                            tAATCGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAATCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAagag                                                                                               <  2:2221137/140‑1 (MQ=255)
                                                      tAGCTATAGGAAGCAGCTTCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTggg                                                                                     >  2:3211628/1‑140 (MQ=255)
                                                            taGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTa                                                                               >  1:1287774/1‑140 (MQ=255)
                                                             aGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAAGCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTAc                                                                              >  2:2956277/1‑140 (MQ=255)
                                                              ggAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTAcc                                                                             <  1:3544477/140‑1 (MQ=255)
                                                                 agcagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCt                                                                          >  2:1648347/1‑140 (MQ=255)
                                                                 agcagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCt                                                                          >  1:930706/1‑140 (MQ=255)
                                                                  gcagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCtt                                                                         >  1:3459638/1‑140 (MQ=255)
                                                                   cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                                        >  2:1487433/1‑140 (MQ=255)
                                                                   cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                                        >  2:2439035/1‑140 (MQ=255)
                                                                   cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                                        >  2:1405088/1‑140 (MQ=255)
                                                                   cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                                        >  2:749817/1‑140 (MQ=255)
                                                                   cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                                        >  2:850569/1‑140 (MQ=255)
                                                                   cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                                        >  2:342757/1‑140 (MQ=255)
                                                                   cagcTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCttt                                                                        >  1:2105383/1‑140 (MQ=255)
                                                                         ctctCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTAc                                                                              >  1:796470/1‑128 (MQ=255)
                                                                           ctcCGTGCAAACAAAATAAGACGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGAGTTGGGGCGGTACCGCTTTATCGGCtt                                                                <  1:1353512/140‑1 (MQ=255)
                                                                           ctcCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCtt                                                                <  1:2530892/140‑1 (MQ=255)
                                                                              cGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCg                                                             >  2:393756/1‑140 (MQ=255)
                                                                               gTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCgg                                                            <  1:1329983/140‑1 (MQ=255)
                                                                                   aaacaaaAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGTGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTAt                                                        <  2:1953763/140‑1 (MQ=255)
                                                                                   aaacaaaAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTAt                                                        <  2:1989826/140‑1 (MQ=255)
                                                                                       aaaaGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATcaca                                                    <  2:3069174/140‑1 (MQ=255)
                                                                                        aaaGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGTGATATTCATGACGACGGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACAt                                                   <  2:3638839/140‑1 (MQ=255)
                                                                                           gaagaagCATACAAGCTGCTAGAAGAAATCGAATAAAGCGGGGGATTTTCATGACGACTGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAAAGAATTTGGGGCGGGACCGCTTTAGCTGATTTTGGCTATACCATTCCg                                               <  1:2505413/140‑1 (MQ=255)
                                                                                           gaagaagCATACAAGCTGCTAGAAGAAATCGAATAAAGCGGGGGATTTTCATGACGACTGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAAAGAATTTGGGGCGGGACCGCTTTAGCTGATTTTGGCTATACCATTCCg                                               <  1:3847221/140‑1 (MQ=255)
                                                                                           gaagaagCATACAAGCTGCTAGAAGAAATCGAATAAAGCGGGGGATTTTCATGACGACTGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAAAGAATTTGGGGCGGGACCGCTTTAGCTGATTTTGGCTATACCATTCCg                                               <  1:1216218/140‑1 (MQ=255)
                                                                                                 gCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTgggg                                                                                    >  1:1440224/1‑98 (MQ=255)
                                                                                                 gCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAga                                          <  2:599228/140‑1 (MQ=255)
                                                                                                 gCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAga                                          <  1:2888942/140‑1 (MQ=255)
                                                                                                 gCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAga                                          <  2:1821652/140‑1 (MQ=255)
                                                                                                 gCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAga                                          <  1:1959894/140‑1 (MQ=255)
                                                                                                 gCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAga                                          <  2:3798205/140‑1 (MQ=255)
                                                                                                 gCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCATGTTTTCAAAGAGAGGATTTGTGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAga                                          >  2:1511479/1‑140 (MQ=255)
                                                                                                      cAAGCTGCTGGATGAACTCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAgaacgaa                                     <  2:357672/140‑1 (MQ=255)
                                                                                                      cAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAGGAGAGGAGGTGGGGCGGTCCCGCTTTATCGGCTTTCGGCTATCCCATTCCGTGAgtccgac                                     >  1:2235922/1‑134 (MQ=255)
                                                                                                      cAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAgaacgaa                                     <  2:874109/140‑1 (MQ=255)
                                                                                                      cAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAgaacgaa                                     <  2:2108254/140‑1 (MQ=255)
                                                                                                      cAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAgaacgaa                                     <  1:3853630/140‑1 (MQ=255)
                                                                                                      cAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAgaacgaa                                     <  1:2821953/140‑1 (MQ=255)
                                                                                                         gctgctGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAg                                  >  1:539044/1‑140 (MQ=255)
                                                                                                           tgcttGATGAAATCGTGTAGGGCGGGGGGATATTCATGACGACCGAACCGTTATTTTTCAAGCCTGTCTTCAAAGCGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAggg                                <  1:969888/140‑1 (MQ=255)
                                                                                                           tgctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAggg                                <  2:2587852/140‑1 (MQ=255)
                                                                                                           tgctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAggg                                <  1:1334924/140‑1 (MQ=255)
                                                                                                           tgctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAggg                                <  1:154853/140‑1 (MQ=255)
                                                                                                           tgctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAggg                                <  1:1648347/140‑1 (MQ=255)
                                                                                                           tgctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAggg                                <  1:3211628/140‑1 (MQ=255)
                                                                                                           tgctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAggg                                <  1:300311/140‑1 (MQ=255)
                                                                                                           tgctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAggg                                <  1:2979872/140‑1 (MQ=255)
                                                                                                           ggctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAggg                                <  2:786188/139‑1 (MQ=255)
                                                                                                            gctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAgggg                               >  2:893456/1‑140 (MQ=255)
                                                                                                            gctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAgggg                               >  2:1794030/1‑140 (MQ=255)
                                                                                                            gctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAgggg                               >  1:2054491/1‑140 (MQ=255)
                                                                                                            gctgGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAgggg                               >  1:3437650/1‑140 (MQ=255)
                                                                                                                gatgttaTCGTGTAGTGCTGTGGGATTTTCATGACGACAGAAGCGTTATTTTTCAAGCGTGTTTTCACAGAGTGGATTTGGGGCGGTACCTCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTg                           <  2:3354195/134‑1 (MQ=255)
                                                                                                                 aTGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGATGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGc                          <  2:1380264/140‑1 (MQ=255)
                                                                                                                 aTGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGc                          <  2:58447/140‑1 (MQ=255)
                                                                                                                 aTGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGc                          <  1:3114836/140‑1 (MQ=255)
                                                                                                                       tCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCt                    >  2:1167347/1‑140 (MQ=255)
                                                                                                                          tgtAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAATGAGAGGATTTGGGGCTGTACCGATTTATCGGCTTTCGGCTATCACATTCCGTCAGAACTAACAGGGGAGTGCTGTGCtttt                 >  2:2332408/1‑140 (MQ=255)
                                                                                                                          tgtAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCtttt                 >  2:279568/1‑140 (MQ=255)
                                                                                                                          tgtAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCAGTAAGAACGAACAGGGGCGTGCTGGGCtttt                 >  2:2269312/1‑140 (MQ=255)
                                                                                                                                 cGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTa                                                                               <  2:1220941/71‑1 (MQ=255)
                                                                                                                                 cGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCTTTTGCTGCTc          >  1:102231/1‑140 (MQ=255)
                                                                                                                                 cGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCTTTTGCTGCTc          >  1:271239/1‑140 (MQ=255)
                                                                                                                                 cGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCTTTTGCTGCTc          >  2:2890345/1‑140 (MQ=255)
                                                                                                                                       gATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCTTTTGCTGCTCATaaaa    <  1:1726128/140‑1 (MQ=255)
                                                                                                                                         tatTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCTTTTGCTGCTCATAAAAAc  >  1:2277575/1‑140 (MQ=255)
                                                                                                                                         tatTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCTTTTGCTGCTCATAAAAAc  >  1:94601/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
AGGGAACCAGCATCTGAAAATATTGCAGATGTTATCCCGAAAACTAATGGATGATAGCTATAGGAAGCAGCTGCTCTCCGTGCAAACAAAAGAAGAAGCATACAAGCTGCTGGATGAAATCGTGTAGTGCGGGGGGATATTCATGACGACAGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAGAGGATTTGGGGCGGTACCGCTTTATCGGCTTTCGGCTATCACATTCCGTCAGAACGAACAGGGGAGTGCTGGGCTTTTGCTGCTCATAAAAAC  >  NZ_CP034943/1242888‑1243164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: