Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,263,998 C→A 11.0% L62F (TTG→TTT fetB ← iron export ABC transporter permease subunit FetB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,263,9980CA11.0% 406.6 / 5.2 181L62F (TTG→TTTfetBiron export ABC transporter permease subunit FetB
Reads supporting (aligned to +/- strand):  ref base C (92/61);  new base A (3/25);  total (95/86)
Fisher's exact test for biased strand distribution p-value = 8.30e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

GAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGATTTTGATAAAAAC  >  NZ_CP034943/1263859‑1264133
                                                                                                                                            |                                                                                                                                       
gcagagACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCGCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCCTCAGc                                                                                                                                         <  2:2520254/138‑1 (MQ=255)
gAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                                                                                         <  1:2513506/140‑1 (MQ=255)
gAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                                                                                         <  1:3706193/140‑1 (MQ=255)
gAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                                                                                         <  1:3870761/140‑1 (MQ=255)
gAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                                                                                         <  2:1664613/140‑1 (MQ=255)
gAAGAGACAGCAGAATTCCCTGCCTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                                                                                         <  1:2709283/140‑1 (MQ=255)
     gaCCGCAGACGTCCCGGCGACACCAGGTCGACACTCGCTAACCCGGCGACCACCCCCCAAACCGACCCGATTGTATTCGACTTTAGTTTGAAAACATCTTGCGCTGCTACTGCCAACATGAACAGCACCATCAGCAAGAt                                                                                                                                    <  2:542514/140‑1 (MQ=255)
      aaagcagAATTCCCTGCTTAAAAATTTATACAATCGATAAATAGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                                                                                   >  1:2955722/3‑140 (MQ=255)
      aCAGCAGAATTCCCTGCGTCACAATTTCTACGATTGCTAACGCGGCAAACACCCTCCAAAACGAACCGAGAGTATTTTTCTTACGCTTGATGACATTTTGCGCCGCCACCGCGAGCATTAACAGCACCATCAGCAGGATa                                                                                                                                   >  1:611823/1‑140 (MQ=255)
      aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                                                                                   >  1:359942/1‑140 (MQ=255)
      aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                                                                                   >  2:581312/1‑140 (MQ=255)
      aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                                                                                   >  2:651699/1‑140 (MQ=255)
      aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACACTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGAAAGATa                                                                                                                                   >  2:3804480/1‑140 (MQ=255)
        cgcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTGTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaaa                                                                                                                                 >  1:910804/2‑140 (MQ=255)
        agcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTTTATTTTTATTTCGTTTGATAACATTTTGCGATGATACTGCCAACATTAAAAGCACCATCAGAAAGAaaaa                                                                                                                                 >  2:2465450/1‑140 (MQ=255)
        agcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAAAGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAAAAGCACCATCAGCAAGATaaa                                                                                                                                 >  2:108896/1‑140 (MQ=255)
         gcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaaaa                                                                                                                                <  2:2614403/140‑1 (MQ=255)
          cagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaaaaa                                                                                                                               >  2:1706591/1‑140 (MQ=255)
                 cccTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATg                                                                                                                        >  1:2091286/1‑140 (MQ=255)
                    tGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGtc                                                                                                                     >  1:3523584/1‑140 (MQ=255)
                    tGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGtc                                                                                                                     >  1:3233435/1‑140 (MQ=255)
                    tGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGtc                                                                                                                     >  1:1506102/1‑140 (MQ=255)
                       gTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTcc                                                                                                                  >  1:3680196/1‑140 (MQ=255)
                          acaATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCcgcg                                                                                                               <  2:2893638/140‑1 (MQ=255)
                          acaATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCcgcg                                                                                                               <  2:1730430/140‑1 (MQ=255)
                          acaATTTATAAAATCGCTAACGCGGCTAACACCCTCAAAAACGAACCGTTTGTTTTTTTATTTATTTTGATAACATTTTGCGCTGCTACTGACAACATTAACAGCACCATAAGAAAGATAAAAACAGGATGGTCTCcgcg                                                                                                               <  2:3286566/140‑1 (MQ=255)
                          aaaaTTTCTACAATCGCTAACGCGGCTAACACCCTCAAAAACGAACCGATTTTATTTTTCTTTCTTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCcgcg                                                                                                               <  2:3462566/138‑1 (MQ=255)
                            aaTTTCTCAAATCGCTACCGCGGCGAACACCCTCCAAAACCAACCCATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGATAATGCCAACATTAACAGAACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGaa                                                                                                             <  2:3424598/140‑1 (MQ=255)
                            aaTTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGaa                                                                                                             <  2:2553240/140‑1 (MQ=255)
                            aaTTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGaa                                                                                                             <  2:1800716/140‑1 (MQ=255)
                                      ctCGCTAACGCGGCTAACACCCTACAAAACCACCCGATTTTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACACCACCATCAGAAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGa                                                                                                   <  2:1328251/139‑1 (MQ=255)
                                      aTCGCTAACGGGGCTAACACCCTCAAAACCGAACCGTTTGTATTTTTCTTTCTTTTTATAACATTTTGCGCTGCTACTGCCAAATTTAACACCAACATAAGAAAGATAAAAACAGGTTGTTCTCCGCGAAAAATAAGCGa                                                                                                   <  2:296472/140‑1 (MQ=255)
                                      aTCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTTTTTTTATTTCGTTTGATAACATTTGGCGCTGCTACTGCCAACTTTAACAGCACCATCAGCAAGATAAAAACTGGATGGTATCCGCGAAAAATAAGCGa                                                                                                   <  2:845176/140‑1 (MQ=255)
                                      aTCGCTAACGCGGCGAACACCCTCAAAAACCAACCGATTTTATTTTTCTTTCTTTTGATAAAATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGTTGGTCTCCGCTAAAAATAAGCGa                                                                                                   <  2:2322769/140‑1 (MQ=255)
                                        cGCTACCGCGGCGAACACCCTCCAAAACGAACCGATTTTATTTTTATTTCGTTTTATAACATTTTGCGCTGATACTGCCAACATTAACAGCACCATCACCAAGATAAAAACAGGATGGTCTCCGCTAAAAATAAGCGACa                                                                                                 <  2:3539673/140‑1 (MQ=255)
                                        cGCTAACGCGGCTAACACCCTCAAAAACGAACCGATTTTATTTTTCTTTGGTTTTATAACATTTTGCGTTGATACTGCAAACATTAACAGCACCATCAGAAAGATAAAAACAGGATGGTCTCGGCGAAAAATAAGCGACa                                                                                                 <  2:487881/140‑1 (MQ=255)
                                             aCGCGGCTAACACCCTCAAAACAGACCCTTTTTTATTTTTTTTTGTTTTGATAACTTTGTGCGCTTTTACGCCAAAAATAAACACCACCATCAGCAATAAAAAAACTGGTTGGTCTCCGCTAAAAATAAGCGACAGTACa                                                                                            <  1:3351378/140‑1 (MQ=255)
                                                 ggctAACACCCTCCAAAACGAACCGATGGTATTTTTATTTCTTTTGATAAAATTTTGCGCTGATACTGCCAACATTAACAGCACCATCACCAATATAAAAACAGGATGGTCTCCGCCAAAAATAAGCGACAGTACATAAc                                                                                        <  2:160949/140‑1 (MQ=255)
                                                 ggctAACACCCAAAAAAACCAACCGTTTTTTTTTTTTTTTCTTTTTATAAATTTTTGCGCTGCTACTCCAAACATTAACACCACCAACACCAACAAAAAAACAGGATGGTCTCCGCCAAAAATAAGCGACAGTACATAAc                                                                                        <  1:1628817/140‑1 (MQ=255)
                                                    gAACACCCTCCAAACCGACCCGTTTGTATTTTTCTTTCTTTTGATAACATTTTGCGCTGATACTGCAAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCga                                                                                     <  2:3737286/140‑1 (MQ=255)
                                                    gAACACCCTCCAAAACGAACCTTTTGTATTTTTCTTTCTTTTTATAACATTTGGCGCTGCTACTGCCAACATTAACCGCACCATCAGCAAGATAAAAACCGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCga                                                                                     <  2:949886/140‑1 (MQ=255)
                                                         cccaaaaaacCGAACCGATTTTTTTTTTATTTCTTTTTATAAATTTTTGCGCTTCTACTCCAAACTTTAACACAACACTAAGAAAAATAAAAACCGGTTGTTCTCCGCTAAAAATAAGCGACAGTACATAACCGATGATa                                                                                <  1:2262962/134‑1 (MQ=255)
                                                         cccTCCAAACCGAACCGTTTGTATTTTTCTTTCGTTTGATAACTTTTTGCGCTGATAGTGCCAACTTTAACAGCACCATCAGCAAGATAAAAACAGTATGGTCTCCGCTAAAAATACGCGACAGTACATAACCGATGATa                                                                                <  2:2302099/140‑1 (MQ=255)
                                                         cccTCCAAAACGAACCTTTGTTTTTTTTTTTTCGTTTTATAACATTTTGCGCTGCTACTGCAAACATTAACCGCACAATCAGAAAGATAAAAACAGGATGGTCTCCGCTAAAAATAAGCGACAGTACATAACCGATGATa                                                                                <  2:1242975/140‑1 (MQ=255)
                                                         cccTCCAAAACGAACCTATTTTATTTTTCTTTCTTTTTATAACATTTTGCGCTGATAATGCCAAAATTAACCGCACCATCAGCAATTTAAAAACTGGATGTTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATa                                                                                <  2:1658519/140‑1 (MQ=255)
                                                         cccTCCAAAACCAACCGTTTGTTTTTTTCTTTCTTTTGATAACATTTTGCGCGGCTACTGCCAACATTAACAGCACCAGCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATa                                                                                <  2:359942/140‑1 (MQ=255)
                                                           ccacAAAACCAACGTATTTTATTTTTTTTTTGTTTTAAAAATTTTGGCGCTTCTACTGCAAAATTAACCCCCACATTCATAAATATAAAAACAGGATGGTATCGGCTAAAAAAAAGCGACAGTACATAACCGATGATAag                                                                              <  1:523100/137‑1 (MQ=255)
                                                           cTCCACAACCACCCTTTTTTATTTTTCTTTGGTTTGATACCATTTGGCGCTGCAACTCCCACAATTAACACCACCATCACCAAGATAAAAACCGGAGGGACACCGCTAAAAATACGCGACAGTACATAACCGATGATAag                                                                              <  1:1671548/140‑1 (MQ=255)
                                                           cTCCAAACCGACCCGATTTTATTTTTCTTTCTTTTGATAACATGTTGCGCTGCTACTGCCAACTTTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAag                                                                              <  2:2286609/140‑1 (MQ=255)
                                                            tCCAAAACTAACCTTTTTTATTTTTCTTTGTTTTTATAACATTTTGCGCTGCTACTGCAAACATTAACAGCACCATCAGCAATATAAAAACAGGATGGTCACCGCTAAAAATAAGCGACAGTACATAACCGATGATAagc                                                                             <  1:1838964/140‑1 (MQ=255)
                                                            tCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagc                                                                             >  2:148134/1‑140 (MQ=255)
                                                            tCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagc                                                                             >  2:2524640/1‑140 (MQ=255)
                                                            tCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagc                                                                             >  2:195096/1‑140 (MQ=255)
                                                            tCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagc                                                                             >  2:1156488/1‑140 (MQ=255)
                                                            accAAAACGACCCTTTTTTTTTTTTCTTTCGTTTGATAACATTTGGCGCTGCTACTCCCAAAATTAACCCCACCATCAGAAATATAAAAACAGGATGGTCTCCGCTAAAAATAAGCGACAGTACATAACCGATGATAagc                                                                             <  1:672742/139‑1 (MQ=255)
                                                            acaaaaaCAAACCTTTTTTATTTTTGTTTCTTTTTATAAATTTTTGCGCTGCTACTGCCAACTTTAACCGAACAATAAGCATTATAAAAACTGTCTGTTCTCCGCCAAAAATAAGCGACAGTACATAACCGATGATAagc                                                                             <  1:491303/137‑1 (MQ=255)
                                                             ccAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagca                                                                            >  1:971720/1‑140 (MQ=255)
                                                             ccAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATGTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagca                                                                            >  1:1899714/1‑140 (MQ=255)
                                                              cAAAACGAACCTTTTGTATTTTTCTTTCTTTTGATAACATTTTGCGCTGATACTGCAAACATTAACAGAACCCTAAGCAATATAAAAACAGGTTGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagcag                                                                           <  2:1226543/140‑1 (MQ=255)
                                                              aaaacccaaCCGATTTTATTTTTTTTTTTTTTTAAAACTTTTGGCGCTGCTCCGGCCAAAATTACCCCCACAATCAGAAAGATAAAAACTGGTGGGTCTCCGCTAAAAAAACGCGACAGTACATAACCGATGATAagcag                                                                           <  1:1745371/133‑1 (MQ=255)
                                                                     caCCTATTGTATTTTTTTTGCGTTTGATAACATTTTCGGCTGCTCCTGCCAACATTAACAGCACCATAAGCAAGCTAACAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATACCCGATGATAAGCAGCTGTAcc                                                                    <  2:3125285/139‑1 (MQ=255)
                                                                     aagcGATTGTTTTTTTCTTTCGTTTTATAACATTTTGCGCTGCTACTGCAAACATTTACAGAACCATCAGAAAGATAAAAACAGGATGGTATCGGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTAcc                                                                    <  2:2921923/137‑1 (MQ=255)
                                                                      ccgtattttattttAATTTCTTTTTAAAAAATTTTTCGCTGATACTGAAAACATTAACACCAACATCACCATTATAAAAACAGTATGGTCTCCGCTAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCg                                                                   <  1:2822544/136‑1 (MQ=255)
                                                                      aCCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCg                                                                   >  1:1176378/1‑140 (MQ=255)
                                                                                  tttgtttcGTTTTATAACATTTGGCGCGCCTACTGCCAAAATTAACAGCACCACCCGAAATAAAAAAACCGGATGGTCTCCGCCAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGAtgg                                                       <  1:122926/140‑1 (MQ=255)
                                                                                   ttctttcGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGAtggt                                                      >  1:1046633/1‑140 (MQ=255)
                                                                                   ttctttcGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGAtggt                                                      >  1:1406884/1‑140 (MQ=255)
                                                                                       ttcGTTTTTTAACATTTTGCGCTGCTACTGCCAACATTAACAGCACACTCACCAAGATAAAAACAGTATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCa                                                  <  2:411165/140‑1 (MQ=255)
                                                                                       ttcGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCa                                                  <  2:2838186/140‑1 (MQ=255)
                                                                                       ttcGTTTGATAAAATTTGGCGCTGCTACTGCCAACATTAACAGCACAATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCa                                                  <  2:3083966/140‑1 (MQ=255)
                                                                                        tcGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCaa                                                 >  1:1015547/1‑140 (MQ=255)
                                                                                        tcGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGAGGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCaa                                                 >  1:3284078/1‑140 (MQ=255)
                                                                                             ttaTAACATTTTGCGCTGCTACTCCAAACATAAACACCACCATCAGAAATAAAAAAACAGGATGGTCTCCGCGAAAAAAAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATc                                            <  1:3093385/138‑1 (MQ=255)
                                                                                             ttaTAACATTTTGCGCTCCTACTGCAAACATTAACACCACAAACCCAAATATAAAAACAGTATGGTCTCCGCCAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATc                                            <  1:817624/138‑1 (MQ=255)
                                                                                             tGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCCGCAAGATAAAACCAGGATGGTCTCCGCGAAAAATACGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATc                                            <  2:1456841/140‑1 (MQ=255)
                                                                                             tGATAACATTTTGCGCGGCGCCTGCCAACATTAACAGCACAATCACCAAGATAAAAACCGGCTGGTCTCCGCCAAAAATACGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATc                                            <  1:2926464/140‑1 (MQ=255)
                                                                                             cgATAACATTGGGCGCGCCACCTGCAAACATAAACCGCACCATCCCCAAGATAAAACCTGGATGTACTCGCCTAAAACTAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATc                                            <  1:3045849/139‑1 (MQ=255)
                                                                                                  acttttgGCGCTGGTACTGACAACATTAACACCAATATAATCAATATAAAAACATGTTGTTGTCCGCAAAAAAAAAGCGAAAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTc                                       <  1:439967/137‑1 (MQ=255)
                                                                                                  aaaTTTTGCGCTGATACTGCCAAAATTAACCCCACCATCAGCAAGATAAAAACAGGATGGTATCCGCTAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTc                                       <  1:2381986/138‑1 (MQ=255)
                                                                                                  aCATTTTGCCCTGCTACTCCAAACATTAACACCACCATCACAACGATAAAAACAGGATGGTCTCCGCTAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTc                                       <  1:195400/140‑1 (MQ=255)
                                                                                                        tGCGCTGCTCCTGCCAACATTAACAGCACCATCAGCAAGATAAAAAGTGTATGGTCTCCGCGAAAAATATGCGACAGTCGATAACCGATGATAAGAAGCTGTACGGCTGCCCGGATGGGTGCAAGGCTCATGTGTTGTTc                                 >  2:2278208/1‑140 (MQ=255)
                                                                                                        tGCGCTGCTACTGCCAACATTAACAGCACCATCCGCAAGATAAAAACAGGATGGTCTCCGCGAAATGTAAGCGACAGTACATACCCGATGATAAGCAGCTGTAGCGGTGCCCGGAGGGTGGCACTGAGCgtgtgtgtgtc                                 >  2:1739574/1‑133 (MQ=255)
                                                                                                              gctACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGcc                           >  2:1966262/1‑140 (MQ=255)
                                                                                                              gctACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGcc                           >  2:1880100/1‑140 (MQ=255)
                                                                                                              gctACTGCCAACATTAAAAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCCCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGAGCATGTCTGTTTCTActcg                           >  2:3020237/1‑137 (MQ=255)
                                                                                                                         cATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATGATGTCTTTTTCTACGCCGGCTTTAAAgg                >  2:3660868/1‑140 (MQ=255)
                                                                                                                         cATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAgg                >  2:2531138/1‑140 (MQ=255)
                                                                                                                            tAACACCACCATCACCAACATAAAAACAGGAGGGTCTCCGCCAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGAtt             <  1:1956030/140‑1 (MQ=255)
                                                                                                                             tacaaccacaATCAGAAAGATAAAAACCGGTTGGTCTCCGCTAAAAATACGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGAtt             <  1:2465450/134‑1 (MQ=255)
                                                                                                                                  gCACCATCAGCACGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGATTTTGAt        <  2:1849355/140‑1 (MQ=255)
                                                                                                                                     ccATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTCAGGGATGTTGATaaa     >  2:2985470/1‑140 (MQ=255)
                                                                                                                                     ccATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGATTTTGATaaa     >  2:733611/1‑140 (MQ=255)
                                                                                                                                     ccATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGATTTTGATaaa     >  1:2492928/1‑140 (MQ=255)
                                                                                                                                     ccATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGATTTTGATaaa     >  2:405189/1‑140 (MQ=255)
                                                                                                                                     ccATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGATTTTGATaaa     >  1:2375898/1‑140 (MQ=255)
                                                                                                                                     ccATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGATTTTGATaaa     >  2:1405294/1‑140 (MQ=255)
                                                                                                                                     ccATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGATTTTGATaaa     >  2:2747277/1‑140 (MQ=255)
                                                                                                                                      caacAGCAAGATAAAAACAGTATGGTCTCCGCCAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGATTTTGATaaaa    <  1:3443438/137‑1 (MQ=255)
                                                                                                                                        tCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGATTTTGATAAAAAc  >  1:2211681/1‑140 (MQ=255)
                                                                                                                                        tCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGAGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGACCTGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGATTTTGATAAAAAc  >  1:1294770/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                       
GAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAATGATCATGTCTTTTTCTACGCCGGCTTTAAAGGATTTTGATAAAAAC  >  NZ_CP034943/1263859‑1264133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: