Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,488,182 G→T 6.8% A575S (GCA→TCA)  ade → adenine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,488,1820GT 6.8% 341.8 / 3.4 124A575S (GCA→TCA) adeadenine deaminase
Reads supporting (aligned to +/- strand):  ref base G (33/79);  new base T (8/3);  total (42/82)
Fisher's exact test for biased strand distribution p-value = 6.31e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

CATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGA  >  NZ_CP034943/1488044‑1488320
                                                                                                                                          |                                                                                                                                          
cATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATattgc                                                                                                                                           >  2:3455011/1‑140 (MQ=255)
          tGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTTATGTAAAATCATTTCACCATATTGCATTGCAATaa                                                                                                                                 >  2:2136998/1‑140 (MQ=255)
          tGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTATCTTTTCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTTATGTAAAATGATTTAAGCAAAATTCATTTCAATaa                                                                                                                                 >  1:3603382/1‑140 (MQ=255)
          tGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGCGTTGCCTGTCATTCCTGACATCAAAAAGACGCCAACAGGAGTATTTGATGTTAAATCCTTTCAGCATATTGCATTGCAATaa                                                                                                                                 >  2:296998/1‑140 (MQ=255)
            ttattaGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTATCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATAATTTCAGCATATTGCATTGCAATaaaa                                                                                                                               >  2:2667676/1‑140 (MQ=255)
            ttattaGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTACTGACATCAAAATTACGACAACAGGATTATTTGATGTAAAATCATTTAAGCAAATTGCATTGCAATaaaa                                                                                                                               >  2:1016593/1‑140 (MQ=255)
             tattaGTCGGTTTCACAGGCTGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATaaaaa                                                                                                                              <  2:3854196/140‑1 (MQ=255)
             tattaGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATaaaaa                                                                                                                              <  2:2497985/140‑1 (MQ=255)
              attaGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCAGTCATTCCAGACATCAAAATTACGACAACAGGATTATTTTATGTAAAAAAATTTCAGCAAATTTCATTTCAaaaaaaaa                                                                                                                             >  1:1873317/1‑140 (MQ=255)
              attaGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGCGTTTCCTTTCATTTCTGACATCAAAAATAAGAGAACAGTAGTATTTGATTTAAAAACAATTGATCATATATAAATTCAATaaaaaa                                                                                                                             >  1:433112/1‑140 (MQ=255)
               ttaGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTATCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTTCAATaaaaaaa                                                                                                                            >  2:559281/1‑140 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAACTGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                         <  1:1367162/140‑1 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                         <  2:2575075/140‑1 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                         <  1:2908486/140‑1 (MQ=255)
                  gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGAt                                                                                                                         <  1:3703982/140‑1 (MQ=255)
                   tCGGTTTCACAGGCGGGTTTAATGCATTTTTGACGCTGTCTTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATg                                                                                                                        <  2:1244001/140‑1 (MQ=255)
                   tCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATg                                                                                                                        <  1:2782642/140‑1 (MQ=255)
                    cGGTTTCACAGGGGGGTTTAATCCATTTTTGACGCTGTCGTTTTTATCTTTGCCTGTCATTCCTGACATCAAAATTACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  2:994415/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:819785/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:958542/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:333050/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:341612/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:738055/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:3455011/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:411092/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:460367/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  2:2024139/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:1460737/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:1702083/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  2:2942018/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  2:2022743/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:2167743/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:2170570/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:2386815/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:2597278/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:2626494/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATGTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:3811981/140‑1 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGCTTTGCCTGTCATTCCTTACATCAAAATGACGACCACAGGATTTTTTGATGTAAAATAATTTCAGCATAATGCATTGCAATAAAAATAGTTGc                                                                                                                       >  2:2533536/1‑140 (MQ=255)
                    cGGTTTCACAGGCGGGTTTAATCCATTTTAGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:580069/140‑1 (MQ=255)
                    cGGGTTCACCGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGc                                                                                                                       <  1:3785847/140‑1 (MQ=255)
                      gTTTCACAGGCGTGTTTAATCAATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTCACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCAc                                                                                                                     <  1:3128825/140‑1 (MQ=255)
                      gTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTATCTTTGCCATTAAATGAAGACAAAAAAAAGAATCCAAAAGGAGTATTTTATGTTAAATCAATTCAGCAAATTACATTTCAAAAAAAAAAAAttaaa                                                                                                                     >  1:1379400/1‑136 (MQ=255)
                      gTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTTCCTGTGATTGCTGACAACAAAATTACGACAACACGCTTATTTTATGTAAAAAAATTTCAGCAAATTTCATTGCAATAAAAAAATATTCAc                                                                                                                     >  1:141394/1‑140 (MQ=255)
                      gTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCAc                                                                                                                     <  1:2114153/140‑1 (MQ=255)
                      gTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCAc                                                                                                                     <  1:848383/140‑1 (MQ=255)
                      gTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGCTTTTCCTGTCATTACTGACATCAAAATGACGACAAAAGGATTATTTGATTTAAAATCATTTAAGCATATTGAATTTCAAAAAAAAAAGATgaaa                                                                                                                     >  1:1193317/1‑137 (MQ=255)
                      gTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGCTTTGCCTGTCATTCCGGACATCAAAATGACGACAACAGGCTTATTTTATGTAAAATAATTTCAGCATATTTCAGTGCAATAAAAAAAGATTCAc                                                                                                                     >  1:3101382/1‑140 (MQ=255)
                                 gggTTTAATCCATTTTTGACGCTGTCGTGTTTAGCTTTGCCCGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTTATGTAAAATCATTTCAGCATATTTCATTTCGATAAAAAAAGATTCACTACAAattgat                                                                                                          >  2:2951575/1‑139 (MQ=255)
                                             tttttGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTTATTTAAAATCATTTCAGCATATTTCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCtttttt                                                                                              >  2:3324010/1‑140 (MQ=255)
                                             tttttGACGCTGTCGTGTTTTGCTTTTCCTGTCATTCCTGACATCAAAATGACGACAGCAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAAATGCAATACAAAGTGAGTGGATCtttttt                                                                                              >  2:3324179/1‑140 (MQ=255)
                                              ttttGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATTACGACAACAGGATTATTTGATTTAAAATAATTTCAGCATATTGCATTGCAATAAAAAAATATGCACTACAAAGTGGGGGCATCttttttt                                                                                             >  2:2769214/1‑140 (MQ=255)
                                              ttttGACGCTGTCGTTTTTAGCGTTGCCTGTCAATCCAGACATCAAAATGACGCCAACACGATTAATTGCTGTAAAAACATTTCAGCATATTGCAGTGCAATAAAAAAAGATTCACTCCAAAGTGCGTGCAGCttttttt                                                                                             >  2:846505/1‑140 (MQ=255)
                                                       tGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTcc                                                                                    <  1:452841/140‑1 (MQ=255)
                                                          cGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCatg                                                                                 <  2:1908967/140‑1 (MQ=255)
                                                          cGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCatg                                                                                 <  2:677442/140‑1 (MQ=255)
                                                          cGTTTTTAGCTTTGCCTGTCATTCCTGAAATCAAAATGACGAAAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCatg                                                                                 <  2:2177898/140‑1 (MQ=255)
                                                                   ctgtGCCTGTCAGTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATCTTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCCTGATGATCGGa                                                                        <  1:1979069/137‑1 (MQ=255)
                                                                   cTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAAATTACATGAATATCggc                                                                        >  2:2937939/1‑139 (MQ=255)
                                                                   cTTTCCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGa                                                                        <  1:2533536/140‑1 (MQ=255)
                                                                     ttGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAg                                                                      <  1:624002/140‑1 (MQ=255)
                                                                     ttGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAg                                                                      <  1:2710405/140‑1 (MQ=255)
                                                                     ttGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAg                                                                      <  1:2733953/140‑1 (MQ=255)
                                                                     ttGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAg                                                                      <  1:2345841/140‑1 (MQ=255)
                                                                               ttCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGa                                                            <  2:474889/140‑1 (MQ=255)
                                                                                      cATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGAGCGGCAGGATCATCGGACGGcgtt                                                     >  1:496144/1‑140 (MQ=255)
                                                                                       aTCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgttt                                                    >  1:2536368/1‑140 (MQ=255)
                                                                                       aTCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgttt                                                    >  1:2863090/1‑140 (MQ=255)
                                                                                               gacgacAACAGGATTATTTGCGGTAAAATCATTTCAGCATATTTCTTTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCGATGATGATCGGAAGGATCATCGGAGTGCGTTTCGtttttt                                            <  1:3433633/140‑1 (MQ=255)
                                                                                               gacgacAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                                            <  1:559281/140‑1 (MQ=255)
                                                                                                         tgATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:2921590/139‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTTCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:1245881/140‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:2144079/140‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:3605649/140‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:1343957/140‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  1:1326055/140‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  1:2165452/140‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:2813041/140‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  1:3275955/140‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  1:770787/140‑1 (MQ=255)
                                                                                                         ggATTATTTGATGTAAAATCATTTCAGCATATTGCATTGAAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:843457/140‑1 (MQ=255)
                                                                                                         gcattattTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa                                  <  2:3082704/138‑1 (MQ=255)
                                                                                                          gATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAAc                                 >  1:101162/1‑140 (MQ=255)
                                                                                                              attTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGgcg                             >  1:619801/1‑140 (MQ=255)
                                                                                                              attTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGgcg                             >  1:153095/1‑140 (MQ=255)
                                                                                                               tttGATGTAAAATCATTTCAGCATATTGCCTTGCAATAAATAAAGATGCACTCCCAAGTGAGTGGATCTTTTTTTTAAACTTCCATGATGATCGGACGGATCATCGGACGGCGTGTCGTTTTGTCATATAAGAAAGgcgc                            >  2:748254/1‑140 (MQ=255)
                                                                                                               tttGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGACGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGgcgc                            >  1:1365855/1‑140 (MQ=255)
                                                                                                               tttGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGgcgc                            >  1:2996045/1‑140 (MQ=255)
                                                                                                               tttGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGgcgc                            >  2:1185527/1‑140 (MQ=255)
                                                                                                               tttGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGgcgc                            >  2:450341/1‑140 (MQ=255)
                                                                                                                  gATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAg                         <  1:28458/140‑1 (MQ=255)
                                                                                                                  gATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAg                         <  2:3603382/140‑1 (MQ=255)
                                                                                                                    tGTAAAATCATTTCAGCATATTTCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTATTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCg                       <  2:1001204/140‑1 (MQ=255)
                                                                                                                    tGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCg                       >  1:1313970/1‑140 (MQ=255)
                                                                                                                    tGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCg                       >  1:232711/1‑140 (MQ=255)
                                                                                                                     gTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCgt                      <  2:141394/140‑1 (MQ=255)
                                                                                                                     gTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCgt                      <  2:1630574/140‑1 (MQ=255)
                                                                                                                                       attgcattgcAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATTATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCAttttt    <  2:317280/140‑1 (MQ=255)
                                                                                                                                       attgcattgcAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCAttttt    >  2:1057824/1‑140 (MQ=255)
                                                                                                                                         tgcattgcaATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATATGAACGGCGCAAGCGTGTCTGGAATCTCATTTTTgt  >  2:2811301/1‑139 (MQ=255)
                                                                                                                                         tgcattgcaATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATAGAAGAACGGCGCCAGCGTGTCTGGAATCTCATTTTTGa  >  2:3364809/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGACATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCATATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGA  >  NZ_CP034943/1488044‑1488320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: