BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,720,712	C→A	14.3%	T212T (ACC→ACA)	spoIIIE →	DNA translocase SpoIIIE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,720,712	0	C	A	14.3%	227.6 / 10.5	117	T212T (ACC→ACA)	spoIIIE	DNA translocase SpoIIIE
Reads supporting (aligned to +/- strand): ref base C (55/42); new base A (2/18); total (57/60)
Fisher's exact test for biased strand distribution p-value = 1.29e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TTGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAG  >  NZ_CP034943/1720573‑1720847

ttGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGTTTTGCATTTATTGATGATATGAAATCCGTCAAAAcc                                                                                                                                         >  2:2705992/1‑140 (MQ=255)

ttGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAAcc                                                                                                                                         >  1:561821/1‑140 (MQ=255)

ttGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAAcc                                                                                                                                         >  1:450648/1‑140 (MQ=255)

ttGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAAcc                                                                                                                                         >  2:517728/1‑140 (MQ=255)

ttGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAAcc                                                                                                                                         >  2:3684411/1‑140 (MQ=255)

ttGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAAcc                                                                                                                                         >  2:3549561/1‑140 (MQ=255)

ttGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAAcc                                                                                                                                         >  2:2824654/1‑140 (MQ=255)

ttGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAAcc                                                                                                                                         >  1:882362/1‑140 (MQ=255)

ttGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTGTGCATTTATTGATGATATGAAATCCGGCAAAAcc                                                                                                                                         >  1:826143/1‑140 (MQ=255)

     aTGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGTACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAatat                                                                                                                                    >  2:1851047/1‑140 (MQ=255)

                   aaTGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATTAAGTCATCGaaaa                                                                                                                      >  1:1491619/1‑140 (MQ=255)

                         tttAGTCACAGGGCGCTCGCCGCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGa                                                                                                                <  2:3275515/140‑1 (MQ=255)

                             gTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAgc                                                                                                            >  2:1496644/1‑140 (MQ=255)

                             gTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAgc                                                                                                            >  1:571115/1‑140 (MQ=255)

                             gTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAgc                                                                                                            >  1:3246091/1‑140 (MQ=255)

                             gTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAgc                                                                                                            >  1:652428/1‑140 (MQ=255)

                             gTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAgc                                                                                                            >  1:2023390/1‑140 (MQ=255)

                                acaGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATTAAATCCGTCAAAACCAAGATTAAGTCATCTAAAAAAACGAAAGCGcc                                                                                                         >  1:3114374/1‑140 (MQ=255)

                                acaGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAACAAAACGAAAGCGcc                                                                                                         >  1:570276/1‑140 (MQ=255)

                                acaGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACTAAAGCTcc                                                                                                         >  1:3609239/1‑140 (MQ=255)

                                acaGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGcc                                                                                                         >  1:3561330/1‑140 (MQ=255)

                                      cgctcgctGCAAGAAACGCTAAAATAGTGGATGAGCCCGATTGGACGTTTTATAAAAGAACAATGGTTAGCATTTATTGATTAAATGAAATCCTTCAAATCAAATATGCAGTCATCGAAAAAAACGAAAGCGCCGAGcaa                                                                                                   >  1:2830441/1‑140 (MQ=255)

                                      cgcTATCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGAATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGcaa                                                                                                   <  1:2409378/140‑1 (MQ=255)

                                              tCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGcaaaaaacaaa                                                                                           >  2:3214263/1‑140 (MQ=255)

                                              tCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGcaaaaaacaaa                                                                                           >  2:977105/1‑140 (MQ=255)

                                              tCAACAAACCCTGAAAAAGTGGATGAGCCAGATTGGCCGCTTTAAAAAAGAACAATGTTTTGCATTTATTGATGAAATGAAAACCTTCAAAAACAATAAGAAGTCATATAAAAAAACGAAAGCGCCGAGcaaaaaacaaa                                                                                           >  2:553046/1‑140 (MQ=255)

                                                aaGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGcaaaaaacaaaaa                                                                                         >  2:3571367/1‑140 (MQ=255)

                                                     aCGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGc                                                                                    >  2:1454241/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGTAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                  >  1:4432/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                  >  1:1135252/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                  >  1:1135306/1‑140 (MQ=255)

                                                                 ttgATGAGCCCTATTGGCCGATTTATAAAATAAAAATTTTTTGAATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACa                                                                        <  2:2348070/138‑1 (MQ=255)

                                                                   gATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGc                                                                      >  2:1499715/1‑140 (MQ=255)

                                                                   gATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGc                                                                      >  2:1211702/1‑140 (MQ=255)

                                                                      taGCCCTTTTGGCCGTTTTATAAAAAAAATTTTTTTTTATTTTTTTTATTATATAAAACCCTAAAAAACAATTATTAACTAATCTAAAAAAACCAACGCGCCCACAAAAAAAAAAAAAACGGCCCGCAAAAAACAGCaaa                                                                   <  1:2306018/139‑1 (MQ=255)

                                                                         cccGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATgg                                                                >  2:3521861/1‑140 (MQ=255)

                                                                              ttGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCg                                                           >  1:590412/1‑140 (MQ=255)

                                                                                ggcctttttAAAAAAGAACATTGGTGTGCATTTGTGTATCATAGTCAATCCTAAAAAACAAATTTGAACACATCGAAAAAAACTAAAGCCCCTAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAAGGGAACCGGa                                                         <  1:2131998/134‑1 (MQ=255)

                                                                                ggCCGTTTTATAAAACAAAATTGGTTTGATTTTTTTGATTATATCAAACCCCAAAAAACAATTATCAAGAAATCTAAAAAAACCAACGCGCCGACAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                         <  1:502706/140‑1 (MQ=255)

                                                                                ggCCGCTTTAAAAAACAAAATTGTTTTGAATTTTTTTATGATTTCAAACCCGAAAAAACAAATATCAAGAAATCAAAAAAAACGAACGCGCCGAGAAAAAAAAAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                         <  1:2498884/140‑1 (MQ=255)

                                                                                ggCCGATTTATAAAATAACATTGTTTTGATTTTATTTTTGATATCAAACCCGACAAAACAAATATGAAGAAATCAAAAAAAACCAACGCCCCGCGAAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                         <  1:1703857/140‑1 (MQ=255)

                                                                                ggCCGATTTATAAAATAACAATGTTTTGCATTTTTTGATTATATGAAATCCTTAAAAACAAATTTGAAGTCATCTAAAAAAACGAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                         <  2:590412/140‑1 (MQ=255)

                                                                                ggCCGATTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAAACCGACAAAACCAATATGAAGTCATCGAAAAAAACGAACCCGCCGAGCAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                         <  1:3776200/140‑1 (MQ=255)

                                                                                ggCCGATTTATAAAACAAATATGTTTTGCATTTTTTTATTATATCAAAACCGAAAAAACAAATATGAAGCAAGCCAAAAAAACGAACCCGCCGACCAAAAAAAAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                         <  1:2994740/140‑1 (MQ=255)

                                                                                ggCCGATTTATAAAACAAAAATGTTTTGAATTTATTTCTCATATCAAACCCGTAAAAACAAATATTAAGTCAGCTAAAAAAACCAAACCGCCCAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                         <  1:185575/140‑1 (MQ=255)

                                                                                gccctcTTTAAAAAACAACATTGGTTTGAATTTATTTATCAAATCAAACCCCAAAAAACAAATATGAAGTCATCGAAAAAAACCAACGCCCCGCCCAAAAACAAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                         <  1:2994748/135‑1 (MQ=255)

                                                                                   ggttttATAAAACAACTATGTTTTCCTTTTTTTGTTTATATCAAATCGTTAAAAAAAAATTTTAAGACTTCTAAAAAAACCACCGGCCCGAGAAAAAAAAAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                      <  1:869236/137‑1 (MQ=255)

                                                                                   ctttttAAAAAACAAAAATGTTTTGATTTTATTTATCATATCAAATCCTTCAAAACAAATTTCAAGTCATCTAAAAAAACGAAAGCGGCCATAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                      <  1:644512/137‑1 (MQ=255)

                                                                                   ctcTTTAAAAAATAACATTGGTTTGAATTTATTGATCATTTGAAATCCGTCAAAACAAATATCCCGTCATCGAAAAAAACGCACGCGCCGCCAAAAAAAAAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                      <  1:107373/138‑1 (MQ=255)

                                                                                   ctatttATAAAATAACAATGGTTTGCATTTATTGATTATATCAAATCCGACAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                      <  2:2477766/137‑1 (MQ=255)

                                                                                   ctatttAAAAAACACCATTGGTTTGCATTTATTGATTATATCAAATCCGAAAAAACCAATATGAAGACATCTAAAAAAACGAACGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                      <  1:770630/137‑1 (MQ=255)

                                                                                   cgttttATAAAAAAAATTTGTTTTGAATTTTTTGATTATATGAAAACCCAAAAAAAAAATATGCCGTCAGCAAAAAAAACCAACGCCCCGACAAAAAAAAAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                      <  1:129663/137‑1 (MQ=255)

                                                                                   cgatttATAAAATAACAATGGTTTGCATTTATTTATGATATCAAAACCGTAAAAACAAATATGAAGTCACCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                      <  1:771459/137‑1 (MQ=255)

                                                                                   cgatttATAAAACAAACATGTTTGGAATTTATTCATGACATGAAAACCTCAAAAACCAATATGACGCAATCGAAAAAAACCAACCCGCCCACCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                      <  1:1155230/137‑1 (MQ=255)

                                                                                   cGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                      <  2:3115272/140‑1 (MQ=255)

                                                                                   cGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTAAAAACCAATATGAAGTCATCGAAAAAAACTAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                      <  2:1580152/140‑1 (MQ=255)

                                                                                   cGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGACAAAACCAATATGAACTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                      <  2:2215778/140‑1 (MQ=255)

                                                                                     cTTTATAAAATAACAATGGTTTTCATTTATTGATGATATGAAATCCTTCAAAACCAATATGAAGTAATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCtt                                                    <  2:1420218/140‑1 (MQ=255)

                                                                                     cTTTATAAAAGAACTATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACTAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCtt                                                    <  2:650670/140‑1 (MQ=255)

                                                                                     cTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATTAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCtt                                                    <  2:46746/140‑1 (MQ=255)

                                                                                     atttATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCtt                                                    <  2:2846793/139‑1 (MQ=255)

                                                                                         ataAAAGAACAATGGTTTGCATTTATTTATGATATGAAATCCGTCAAAACCAATATGAACTCATCGAAAAAAACGAAACCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTctgc                                                <  2:3777649/140‑1 (MQ=255)

                                                                                             aacaaCAATGGTTTGCATTTATTGATGATATGACAGCCGTCAAAACCAATAGGAAGTCATCGAAAAAAACGAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGaa                                            <  2:162722/137‑1 (MQ=255)

                                                                                               gAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAaga                                          <  2:3246091/140‑1 (MQ=255)

                                                                                                aaaaaTGGTTTGCATTTATTTATGATATGAAATCCTTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCCCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAagag                                         <  2:1997206/137‑1 (MQ=255)

                                                                                                aaCAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGACGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCACATGGAACCGGAGCCTTCTGCTGAagag                                         >  1:3529158/1‑140 (MQ=255)

                                                                                                aaCAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAagag                                         <  2:2826018/140‑1 (MQ=255)

                                                                                                  cAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCACATGGAACCGGAGCCTTCTGCTGAAGAggg                                       >  1:1298004/1‑140 (MQ=255)

                                                                                                        tttGCATTTATTGAGGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACTAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGAttt                                 <  2:207940/140‑1 (MQ=255)

                                                                                                        tttGCATTTATGGCTGATATGAAATCCGTCAAACCCTATATTACTTAATCTAAAAAAACTAATCCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGAttt                                 <  2:453332/140‑1 (MQ=255)

                                                                                                          tGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                               >  1:287520/1‑140 (MQ=255)

                                                                                                          tGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                               >  1:2697402/1‑140 (MQ=255)

                                                                                                            cATTTATTGATTATCTTAAAGCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGaa                             <  2:1722013/140‑1 (MQ=255)

                                                                                                            aaTTTATTGATGATATGAAAACCGTCAAAACAAATATTAAGACATCGAAAAAAACGAAAGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGaa                             <  1:2855303/139‑1 (MQ=255)

                                                                                                             cttttttGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCACAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGaaa                            <  2:1191053/139‑1 (MQ=255)

                                                                                                             aTTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCTAAAAAAACGAACGCGCCGAGCAAAAAACAAAAAACGGCCCACAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGaaa                            <  2:1191060/140‑1 (MQ=255)

                                                                                                                   tgatgaTATTACATCCGTCAAAACCAATATGAAGTCAACGAAACAAACCAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACCGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGCTTTGGAAACGGTAt                      <  2:1859498/140‑1 (MQ=255)

                                                                                                                    gatgatATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGACGAGGGGGATTTGGAAACGGTATc                     >  2:3817311/1‑140 (MQ=255)

                                                                                                                       gatATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGcc                  >  2:1744397/1‑140 (MQ=255)

                                                                                                                        atatTAAATCCTTCAAAACCAATATGAAGTCATCTAAAAAAACCAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                 <  2:1251721/140‑1 (MQ=255)

                                                                                                                        atatTAAATCCGTCAAAACCAATATGAAGTCATCTAAAAAAACGAACGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                 <  2:1451237/140‑1 (MQ=255)

                                                                                                                        atatCAAATCCGTCAAAACAAATATGAAGTCATCGAAAAAAACTAAAGCCCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                 <  2:1022588/140‑1 (MQ=255)

                                                                                                                        agatGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                 <  2:261801/138‑1 (MQ=255)

                                                                                                                        agatGAAAACCATCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                 <  2:1622282/138‑1 (MQ=255)

                                                                                                                          tttaaaGCCGTAAAAAACAATATGAATTAATATAAAAAAACTAAAGCTCCGAGAAAAAAAAAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCctct               <  1:187825/137‑1 (MQ=255)

                                                                                                                          cttaaaTCCTTAAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGTGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCctct               <  2:446290/137‑1 (MQ=255)

                                                                                                                          atGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCctct               >  2:876850/1‑140 (MQ=255)

                                                                                                                          atGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCctct               >  2:2746930/1‑140 (MQ=255)

                                                                                                                             aaaTCCTTAAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat            <  2:882362/140‑1 (MQ=255)

                                                                                                                             aaaTCCGTCAAAACCAATATGACGTCATCGAAAAAAACCAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCACAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat            <  2:2571579/140‑1 (MQ=255)

                                                                                                                             aaaTCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat            <  2:2406070/140‑1 (MQ=255)

                                                                                                                              aaTCCGTAAAAACCAATATGAAGTAATCTAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTatt           <  2:465531/140‑1 (MQ=255)

                                                                                                                              aaTCCGTAAAAACCAATAGGAACTCATCGAAAAAAACGAAAGCGCCGAGCAACAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTatt           <  2:3766694/140‑1 (MQ=255)

                                                                                                                               aTCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTattc          >  2:1070780/1‑140 (MQ=255)

                                                                                                                               aTCCGTCAAAACCAATAAGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTattc          >  2:1919932/1‑140 (MQ=255)

                                                                                                                                  gttaaaatCCATTTTGAAGTCATCTAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGACGAGGGGGATTTGGAAACGGTATCGCCTCTTattcatt       <  2:1457235/136‑1 (MQ=255)

                                                                                                                                  cGTCAAAACCAATCTGACGTCATCGAAAAAAACGAAAGCGCCTAGCAAAAAACAAAAAACGGCCCGCAACAAACAGCAAATGGAACCGGAGCCGTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTattcatt       <  2:1344769/140‑1 (MQ=255)

                                                                                                                                   gTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGaa                                            >  1:987690/1‑102 (MQ=255)

                                                                                                                                    tCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCTCCTCTTAttcattca     >  2:2144342/1‑140 (MQ=255)

                                                                                                                                       aaaCCAATATGAAGACATCGAAAAAAACGAAAGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCagag  <  2:1130833/140‑1 (MQ=255)

TTGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAG  >  NZ_CP034943/1720573‑1720847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`