Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,753,584 G→T 12.6% intergenic (+275/‑230) fabD → / → EO946_RS09090 ACP S‑malonyltransferase/acyltransferase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,753,5840GT12.6% 595.7 / 12.1 265intergenic (+275/‑230)fabD/EO946_RS09090ACP S‑malonyltransferase/acyltransferase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (78/142);  new base T (43/2);  total (121/144)
Fisher's exact test for biased strand distribution p-value = 7.08e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

AAAGAGGCTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTT  >  NZ_CP034943/1753447‑1753718
                                                                                                                                         |                                                                                                                                      
aaaGAGGCTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTATTTGTTGAt                                                                                                                                      >  2:140693/1‑140 (MQ=255)
 aaGAGGCTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATc                                                                                                                                     <  1:3264410/140‑1 (MQ=255)
 aaGAGGCTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATc                                                                                                                                     <  1:2147610/140‑1 (MQ=255)
  agagGCTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCATCCGGCATATCATGATATACCATCGCTCTCAACTCAAGATGGACTTCTATTTAACCGCAACCAAAAATTAGAGTCGATTTTCTTTGTTGATca                                                                                                                                    >  1:2452902/1‑139 (MQ=255)
  agagGCTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTGTTTATcc                                                                                                                                    >  1:2507313/1‑140 (MQ=255)
  agagGCTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGAtgc                                                                                                                                    >  1:1031136/1‑138 (MQ=255)
  agagGCTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATcc                                                                                                                                    >  1:788843/1‑140 (MQ=255)
  agagGCTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGGTCTTTTTTTATcc                                                                                                                                    >  1:1242690/1‑140 (MQ=255)
  agagGCTGATAAAACATGTCCACACGAGATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTCTTTAACCGCACCCAACTCTTAGAGTCGATGTTCTttgttgttgc                                                                                                                                    >  1:3435222/1‑136 (MQ=255)
     ggCTGATAAAACAGGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGt                                                                                                                                 <  1:540253/140‑1 (MQ=255)
      gCTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTc                                                                                                                                >  1:807370/1‑140 (MQ=255)
       cTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCa                                                                                                                               <  2:2318311/140‑1 (MQ=255)
       cTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCa                                                                                                                               >  1:957670/1‑140 (MQ=255)
       cTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTCTTTGTTTATCCGGTCa                                                                                                                               >  2:3203686/1‑140 (MQ=255)
       cTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTCTTTGTTTATCCGGTCa                                                                                                                               >  2:795794/1‑140 (MQ=255)
        tGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTTTCCTGTAAt                                                                                                                              >  2:283698/1‑140 (MQ=255)
        tGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGTTCCTGTCAt                                                                                                                              >  1:626098/1‑140 (MQ=255)
        tGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTATTTTTTGATGCTGTCAt                                                                                                                              >  1:910578/1‑140 (MQ=255)
                    tCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTCACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCa                                                                                                                  <  1:3114645/140‑1 (MQ=255)
                         acGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAAtttt                                                                                                             >  1:1077701/1‑140 (MQ=255)
                               tttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTGTTTTTCCTGTCATTATTTTATATAAATTTTGAGtta                                                                                                       >  2:1671100/1‑140 (MQ=255)
                               tttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTTGTCCTGTCATTATTTTATATAAATTTTGAGata                                                                                                       >  2:1649522/1‑140 (MQ=255)
                               tttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGata                                                                                                       <  1:2322141/140‑1 (MQ=255)
                                ttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTACCCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGatat                                                                                                      <  2:769213/140‑1 (MQ=255)
                                ttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGatat                                                                                                      <  1:3658141/140‑1 (MQ=255)
                                ttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGatat                                                                                                      <  2:431353/140‑1 (MQ=255)
                                ttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTATATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGatat                                                                                                      <  2:2541593/140‑1 (MQ=255)
                                   aaCTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGAt                                                                                                                                      >  2:2806125/1‑105 (MQ=255)
                                   aaCTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATATTAGAGTCGATTTTATTTTTTTATACTGTCATTATTTTATATCAATTTTGACATATaaa                                                                                                   >  2:884757/1‑140 (MQ=255)
                                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                 <  1:283698/140‑1 (MQ=255)
                                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                 <  1:421725/140‑1 (MQ=255)
                                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                 <  1:3699910/140‑1 (MQ=255)
                                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                 <  2:3749788/140‑1 (MQ=255)
                                      tATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACAt                                                                                                <  1:1935642/140‑1 (MQ=255)
                                      tATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACAt                                                                                                <  1:38887/140‑1 (MQ=255)
                                      tATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACAt                                                                                                <  1:2239482/140‑1 (MQ=255)
                                       aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTATAGTCGAGGTTGTTTGTTTATCCTTGGATTATGGTATAACAATTTTGAGTTAACAAAATa                                                                                               >  1:1631599/1‑140 (MQ=255)
                                       aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATTTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTTAGATATAAAAATa                                                                                               >  2:3019544/1‑140 (MQ=255)
                                       aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGatat                                                                                                      <  2:3535493/133‑1 (MQ=255)
                                       aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATACTGTCATTATTTTATATCACTTTTGAGATATCAAAATa                                                                                               >  1:254366/1‑140 (MQ=255)
                                       aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTGCTTTGTTGATCCTGTCATTATGTTATATCAAATTTGAGAAATCAACaaa                                                                                               >  2:2639084/1‑138 (MQ=255)
                                        tGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTGTTGATCCTGTCATTATTTTATATCAAATTTTATATATCAACATaa                                                                                              >  1:2491186/1‑139 (MQ=255)
                                        tGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTCTTTGTTTATCCTGTCATTATGTTATATGAATTTTTATATATCaaaaaaa                                                                                              >  1:3374252/1‑135 (MQ=255)
                                             gtcagCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTGTTTATCCTGTCATTATTTTATATCAATTTTGAGATATCAAAATAccccga                                                                                         >  1:2828412/1‑138 (MQ=255)
                                              tcagCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGGTGATCCTGTCATTAAGTTAAATAAATTTTGAGATATCAAAATAAACCTAg                                                                                        >  2:862179/1‑140 (MQ=255)
                                                agacttcATATCATGATAGAACATAGCTATCAACTCAAGATGGCCTTCTATTTAACCGAACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                      <  2:1358816/134‑1 (MQ=255)
                                                agCCGGCATATCATGATATACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATATTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                      <  2:3871448/140‑1 (MQ=255)
                                                agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                      <  1:715791/140‑1 (MQ=255)
                                                agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                      <  2:1868873/140‑1 (MQ=255)
                                                agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                      <  1:229792/140‑1 (MQ=255)
                                                agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                      <  1:319598/140‑1 (MQ=255)
                                                agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                      <  1:2370753/140‑1 (MQ=255)
                                                agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGAGGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                      <  1:1994354/140‑1 (MQ=255)
                                                 gCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAcc                                                                                     <  1:1328529/140‑1 (MQ=255)
                                                  ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTATACTGTCATTATGTTATATCAATTTTGAGATATAAACAAAAACCTAGACCa                                                                                    >  2:715791/1‑140 (MQ=255)
                                                  ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATGAAAAAACACCTAGACCa                                                                                    >  2:996789/1‑140 (MQ=255)
                                                  ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                      >  1:2869744/1‑138 (MQ=255)
                                                  ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCa                                                                                    <  2:1834875/140‑1 (MQ=255)
                                                  ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTATTTGTTGATACTGTCATTATGTTATATAAATTTTGAGATATCAACATAAAACTAGAGCa                                                                                    >  1:2745735/1‑140 (MQ=255)
                                                  ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTGTTTGTTGCTCCGGTCATTATGTTATATCCATTTTGTGTTATCTACATACACCGAGAccc                                                                                    >  1:3554802/1‑139 (MQ=255)
                                                            aTGAGAGACCCTCGCTCTCAACTCAAGATGGCCTTCTAGTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCataata                                                                          <  1:2969460/140‑1 (MQ=255)
                                                                 aGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGcc                                                                     <  2:1493405/140‑1 (MQ=255)
                                                                 aGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGcc                                                                     <  1:862179/140‑1 (MQ=255)
                                                                 aGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGACATATCAACATACACCTAGACCACTTCATAATAGAGcc                                                                     <  2:2655909/140‑1 (MQ=255)
                                                                   aCCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGGCATTATGTTATATCAATTTTTTGATATCAACATAAAACTAGACCACTTCATAATAGAGCCGc                                                                   >  1:775213/1‑140 (MQ=255)
                                                                   aCCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTGTTTGTTGATCCTGTCATTATGTTATATCAATTTTGTGATATCAAAATACACCTAGACCACTTCATAATAGAGCCGc                                                                   >  2:1805929/1‑140 (MQ=255)
                                                                       tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTTTTGATCCTGTCATTATTTTATATCAATTTTTTGATATCAAAATAAACCAACACCCATTCATAATATAGCCGCAtac                                                               >  2:1166529/1‑138 (MQ=255)
                                                                       tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTGATCCAGTCATTATGTTATATCAATTTTGAGATATCAACATAAACCTAGACCACTTCATAATATAGCCGCAtac                                                               >  2:289458/1‑138 (MQ=255)
                                                                        cGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTGTTTTTGCTGTCCTTAATTTTTAGGAATTTTTATATATAAACATAAACATATACCACTTCATAAAAGAGACCCATACa                                                              >  1:2057703/1‑140 (MQ=255)
                                                                        cGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAAATTTTAGATATAAACAAACACCTAGAACAAATCAAAATAGAGCCCCATCCa                                                              >  2:1041327/1‑140 (MQ=255)
                                                                        cGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGGTGTTTGTTTTTCCTGTAATTAATTTATATGCATTTTTAGATATAAACATAAACCTAGACCACGTACTAATAAAGCCGCAGccc                                                              >  1:563600/1‑139 (MQ=255)
                                                                          ctctCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTGTTGATGCTGTCATTATGTTATTTCAATTTTGATATATCAAAATACACCTGGACCACTTAATAATAGAGCCGCATCCaga                                                            >  2:1591441/1‑140 (MQ=255)
                                                                          ctctCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATAAACATACACCTAGACCACTTAATAATAGAGCCGCATACaga                                                            >  2:691466/1‑140 (MQ=255)
                                                                             tcaactcaaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGTTTGTTTTTTGTTCCTGTCATTATTTTATATCAATTTTGAGATATCAACATAAACCAAGAACACTTAATAAAAGAGCCGCATACAGAGCa                                                         >  2:3700456/1‑140 (MQ=255)
                                                                                 ctcaaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGGTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCaa                                                     <  2:3151572/140‑1 (MQ=255)
                                                                                  tcaaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCaaa                                                    <  1:1671100/140‑1 (MQ=255)
                                                                                     aGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTGTTTTTTGATCCGGTCAATAAGTTATATAAATTTTTTGATATCAACATACAACTAGACCACAAGATAATAGAGGCGCATCCAGAGCACCaaaaaa                                                 >  1:3023618/1‑138 (MQ=255)
                                                                                      gATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGAACCAAAAGCa                                                <  2:2653121/140‑1 (MQ=255)
                                                                                       aTGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCaa                                               <  1:11922/140‑1 (MQ=255)
                                                                                         ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTTTTGATACTGTCATTATGTTATATAAATTTTTAGATATCAACATAAACCAAAAACACTTCATAAAAGAGACGCATACAGAGCACCAAAAGCaaac                                             >  1:2064201/1‑138 (MQ=255)
                                                                                         ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATGCTGTCATTATTTTATATAAATTTTGAGTTATCAACAAAAACCTATAACACATCATAATAGAGCCGCATCCAGAGCACCAAAAGCAAtc                                             >  1:1737971/1‑140 (MQ=255)
                                                                                         ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTTTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAAtc                                             <  2:3435222/140‑1 (MQ=255)
                                                                                         ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAAtc                                             <  2:608629/140‑1 (MQ=255)
                                                                                         ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGCTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAAtc                                             <  2:1242690/140‑1 (MQ=255)
                                                                                         ggCCTTATATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTGGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAAtc                                             <  2:329986/140‑1 (MQ=255)
                                                                                         agacagcTATTTAACCGCACCCAAATCTTAGAGTCTATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAAtc                                             <  2:308638/134‑1 (MQ=255)
                                                                                           ccTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTGTTTTTTTATCCAGTGATTATGTTATATAAATTTTGATATATAAAAATAAAACTAGACCACTTGATAATATATCCGCAAACAGAGCAACAAAAACaaaact                                           >  1:1464361/1‑136 (MQ=255)
                                                                                              tCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCAATTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATcctcct                                        <  1:3838415/140‑1 (MQ=255)
                                                                                              tCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTATTTGTTGATCCTGTCATTATTTTATATCAATTTTGAGATATCAACATAAACCTAGACCCCTTCATAAAAGTGCCGCATCCAGAGCACCAAAATCAATcctgct                                        >  2:2253205/1‑140 (MQ=255)
                                                                                                       ccGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGa                               <  1:2932499/140‑1 (MQ=255)
                                                                                                       ccGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGa                               <  2:1165559/140‑1 (MQ=255)
                                                                                                        cGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGAt                              <  1:2469097/140‑1 (MQ=255)
                                                                                                        cGCACCCAAAACTTAGAGTCGATTTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGAt                              <  1:1304450/140‑1 (MQ=255)
                                                                                                          cACCCAAATCTTAGAGTCGATGTTGGTTTTTGATGCTGTCATTATTTTATATAAATTTTTAGATATAAAAATACACCTAGAACACTTGATAAAATAGCCGCATCCAGAGCACCAAAAGCAAACCTCCGATTAGTTGGtta                            >  1:228062/1‑139 (MQ=255)
                                                                                                          cACCCAAATCTTAGAGTCGATGTTCTTTGTTGATACTGTCAATATGTTATATGAAATTTTAGATATCAACATACAACAAGACCAAATAATAATATAGCCGCATACAGAGCACCAAAAGAAAAACTACAAATAGATGAttc                            >  2:2506199/1‑140 (MQ=255)
                                                                                                            cccAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAAAATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGAttctt                          >  2:3102743/1‑140 (MQ=255)
                                                                                                                     tAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTAttttt                 <  1:1109892/140‑1 (MQ=255)
                                                                                                                     tAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTGTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTAttttt                 <  2:437193/140‑1 (MQ=255)
                                                                                                                            gATGTTCTTTGTTTATCCTGTCATTATGTTATATCAATTTTTAGATATCAAAATACACCAAGACCACTTCATAATAGAGCCGCATACAGAGCAACAAAATCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACAATa          >  2:943877/1‑140 (MQ=255)
                                                                                                                             aTGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTAt         <  2:1865532/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
AAAGAGGCTGATAAAACATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTT  >  NZ_CP034943/1753447‑1753718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: