Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,753,622 C→A 7.0% intergenic (+313/‑192) fabD → / → EO946_RS09090 ACP S‑malonyltransferase/acyltransferase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,753,6220CA 7.0% 742.8 / 6.0 283intergenic (+313/‑192)fabD/EO946_RS09090ACP S‑malonyltransferase/acyltransferase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (60/189);  new base A (32/2);  total (92/191)
Fisher's exact test for biased strand distribution p-value = 1.07e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

CTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCGCTGCTGTGTATTGTA  >  NZ_CP034943/1753484‑1753757
                                                                                                                                          |                                                                                                                                       
cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                                        <  1:3651909/140‑1 (MQ=255)
cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                                        <  1:135064/140‑1 (MQ=255)
cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                                        <  1:2472497/140‑1 (MQ=255)
cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                                        <  1:2431433/140‑1 (MQ=255)
cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                                        <  1:2309268/140‑1 (MQ=255)
cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                                        <  2:3749751/140‑1 (MQ=255)
cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                                        <  2:421369/140‑1 (MQ=255)
cTATGTCAGTCAGCCGGCATATAATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCACATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                                        <  1:54990/140‑1 (MQ=255)
 tATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACAt                                                                                                                                       <  1:39323/140‑1 (MQ=255)
  aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATTTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTTAGATATAAAAATa                                                                                                                                      >  2:3019544/1‑140 (MQ=255)
  aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTGATTATGTTATATCAATTTTGATATATAAAAATa                                                                                                                                      >  2:1109892/1‑140 (MQ=255)
  aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCGGTCATTATGTTATATCAATTTTGTGATATGAACATa                                                                                                                                      >  1:78900/1‑140 (MQ=255)
  aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATACTGTCATTATTTTATATCACTTTTGAGATATCAAAATa                                                                                                                                      >  1:254366/1‑140 (MQ=255)
  aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTGTTTGTTTGTCCTGTCATTATGTTATATCAATTTTGAGATATCAAAATa                                                                                                                                      >  1:1036277/1‑140 (MQ=255)
          cagCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAACTCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGa                                                                                                                              <  2:1225882/140‑1 (MQ=255)
          cagCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGa                                                                                                                              <  2:901965/140‑1 (MQ=255)
           agCCGGCATATCATTATAGACCATCGCTGTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                                             <  2:3087624/140‑1 (MQ=255)
           agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                                             <  1:1212979/140‑1 (MQ=255)
           agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                                             <  1:2370753/140‑1 (MQ=255)
           agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                                             <  1:1735565/140‑1 (MQ=255)
           agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                                             <  1:1662103/140‑1 (MQ=255)
           agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                                             <  1:319598/140‑1 (MQ=255)
           agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGAGGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                                             <  1:1994354/140‑1 (MQ=255)
             ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTATACTGTCATTATGTTATATCAATTTTGAGATATAAACAAAAACCTAGACCa                                                                                                                           >  2:715791/1‑140 (MQ=255)
             ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATGAAAAAACACCTAGACCa                                                                                                                           >  2:996789/1‑140 (MQ=255)
             ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCa                                                                                                                           <  2:1834875/140‑1 (MQ=255)
             ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTGTTTGTTGCTCCGGTCATTATGTTATATCCATTTTGTGTTATCTACATACACCGAGAccc                                                                                                                           >  1:3554802/1‑139 (MQ=255)
                       aTGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCataata                                                                                                                 <  1:2461461/140‑1 (MQ=255)
                       aTGAGAGACCCTCGCTCTCAACTCAAGATGGCCTTCTAGTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCataata                                                                                                                 <  1:2969460/140‑1 (MQ=255)
                              aCCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTTGTGATACTGTGATTATGTTATAACAATTTTGTGATATACAAAAACACCTAGACCACTTCATAAAAGAGCCGc                                                                                                          >  1:2847574/1‑140 (MQ=255)
                              aCCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGGTCTTTGTTGATCCAGGCATTATGTTAAATCAATTTTGATATATCAAAATAAACCTAGACCACATCATAATAGAGCCGc                                                                                                          >  2:1272155/1‑140 (MQ=255)
                                  tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTTTTGATCCTGTCATTATTTTATATCAATTTTTTGATATCAAAATAAACCAACACCCATTCATAATATAGCCGCAtac                                                                                                      >  2:1166529/1‑138 (MQ=255)
                                     ctctCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTACTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCaga                                                                                                   <  1:3700456/140‑1 (MQ=255)
                                     ctctCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTCTTTGTTGTTCCTGGCATTATGTTATATCAATTTTGAGATATAAAAATACACCTAAACCACTTCATAATAGAGCCGCATCCaga                                                                                                   >  2:459650/1‑140 (MQ=255)
                                     ctctCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATAAACATACACCTAGACCACTTAATAATAGAGCCGCATACaga                                                                                                   >  2:691466/1‑140 (MQ=255)
                                            ctcaaGATGGCCTTCTATTTAACCGCCCCCACCTCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCCATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCaa                                                                                            <  2:3144909/140‑1 (MQ=255)
                                               aaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTGATCCTGTCATTATTTTATATCAATTTTGAGATATAAACATAAAACAAGACCACATAATAAAAAAGCCGCATACAGAGCACCAAAAg                                                                                         >  2:2442763/1‑140 (MQ=255)
                                               aaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTTAGATATAAACAAACACCTATAACAATTGATAATAGATCCGCATCCACAGCACCaaaac                                                                                         >  2:1867088/1‑139 (MQ=255)
                                               aaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                                             <  1:640303/104‑1 (MQ=255)
                                                aGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTTTTTTTCCTTTCATTATGTTATATGAATTTTGAGATATAAACAAACAACGACACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGc                                                                                        >  1:2262396/1‑140 (MQ=255)
                                                aGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTCTTTGTTTATCCTGTCCTTATGTTATATGAATTTTGTGATATCAACCTACGACTGGACCACGGCATAATAGAGCCGCATCCAGAGGCCCAAAAGc                                                                                        >  1:289880/1‑140 (MQ=255)
                                                 gATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCa                                                                                       <  2:2645023/140‑1 (MQ=255)
                                                 gATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCa                                                                                       <  2:324987/140‑1 (MQ=255)
                                                 gATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCa                                                                                       <  1:976291/140‑1 (MQ=255)
                                                    ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTTTTACTGTCATTATGTTATATAAATTTTGAGATATAAACATAAACCTAGACCACGTCATAATAGAGCCGCCTCCCGAGCCCCAAAAGCacac                                                                                    >  1:2063748/1‑137 (MQ=255)
                                                    ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTTTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAAtc                                                                                    <  2:3435222/140‑1 (MQ=255)
                                                    ggCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAAtc                                                                                    <  2:608629/140‑1 (MQ=255)
                                                         tCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATcctcct                                                                               <  1:3123094/140‑1 (MQ=255)
                                                         tCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCAATTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATcctcct                                                                               <  1:3838415/140‑1 (MQ=255)
                                                          cTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTa                                                                              <  1:1325346/140‑1 (MQ=255)
                                                                  ccGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGa                                                                      <  1:723245/140‑1 (MQ=255)
                                                                  ccGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGa                                                                      <  1:2932499/140‑1 (MQ=255)
                                                                     cACCCAAATCTTAGAGTCGATGTTGTTTGTTTATGCTGTCATTATGTTTTATAAATTTTGAGATATCAAAATACACCTAGACCACTTCATAATATAGCCGCATACCGAGCACCAAAAACAATCCTCCGATTAGTTGAttg                                                                   >  1:110497/1‑139 (MQ=255)
                                                                     cACCCAAATCTTAGAGTCGATGTTCTTTGTTGATACTGTCAATATGTTATATGAAATTTTAGATATCAACATACAACAAGACCAAATAATAATATAGCCGCATACAGAGCACCAAAAGAAAAACTACAAATAGATGAttc                                                                   >  2:2506199/1‑140 (MQ=255)
                                                                     cACCCAAATCTTAGAGTCGAGGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATAAACATAAAACTAGAACACATCATAAAATAGCCGCATACAGAGCACCAAAAGCAATACAACTATTAGTTGAtta                                                                   >  2:1668394/1‑139 (MQ=255)
                                                                       cccAAATCTTAGAGTCGAGGTTCTTTTTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCCAAAGCAAACCTCCGATTAGTTGAttctt                                                                 >  2:2869547/1‑140 (MQ=255)
                                                                                 agagTCGATGTTCGTTGTTGATCCTGTCATTATGTTATATCAAATTTTAGATATCAACAAAAACCTAAACCACTTCATAATATAGCCGCATCCAGAGCCCCAAAATCAATCCTCCTATTAGTTGATTCTTCTTAtttttt                                                       >  2:549982/1‑140 (MQ=255)
                                                                                      cGATGTTCTTTTTTGTTACTGTCATTATGTTATATCCATTTTTAGATATCAAAATACACCTATAACACATCATAATATAGCCGCCTCCAGAGCAACAAAATCAATCCTCCAATTAGTTGATTGTTCTTATTTTTTTCAtt                                                  >  2:1429259/1‑140 (MQ=255)
                                                                                       gATGTTCTTTGTTTATCCTGTCATTATGTTATATCAATTTTTAGATATCAAAAAAAAACAAAAACACATAATAATAGAGCCCCAAACAGAGCAACAAAATCAAACCGCCAATTAGTTTATTCTTGATATTTTTTaaaata                                                 >  2:3388564/1‑135 (MQ=255)
                                                                                       gATGTTCTTTGTTGATCCTGTCATTATGTTAAATCAATTTTGAGATATCAACATAAACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAACCAAGCCGCCTAATAGTTGATTCTTCTTATTTTTTAAATTa                                                 >  2:3051474/1‑140 (MQ=255)
                                                                                             cTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCtt                                           <  1:1619098/140‑1 (MQ=255)
                                                                                               ttgttgATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCtttt                                         <  2:1545101/140‑1 (MQ=255)
                                                                                               ttgttgATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCtttt                                         <  2:3815671/140‑1 (MQ=255)
                                                                                                          gTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAatat                              <  2:2262396/140‑1 (MQ=255)
                                                                                                          gTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAatat                              >  2:2608337/1‑140 (MQ=255)
                                                                                                          gTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAACCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAatat                              <  2:289880/140‑1 (MQ=255)
                                                                                                          gTCATTATGTTATATCAATTGTGAGATATCAACATACACCGAGACCACGTCATAAGCGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAatat                              <  1:1304222/140‑1 (MQ=255)
                                                                                                              ttATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCATATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATcaca                          <  1:804375/140‑1 (MQ=255)
                                                                                                                aTGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACAtt                        >  2:1871186/1‑140 (MQ=255)
                                                                                                                     atatCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACt                   <  1:1872840/140‑1 (MQ=255)
                                                                                                                       ataaaTTTTGAGATATCAAAATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTTTGTCTTTTCAATTAAATATCACATTTCACTCg                 <  1:894232/137‑1 (MQ=255)
                                                                                                                       atCAATTTTGAGATATCAACATACACCTATACCACTTCATACTAGAGGCGCATCCAGAGCACCAAACGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCg                 <  1:1282546/140‑1 (MQ=255)
                                                                                                                       atCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCg                 <  1:2301539/140‑1 (MQ=255)
                                                                                                                       atCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCg                 <  1:2772448/140‑1 (MQ=255)
                                                                                                                       atCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCg                 <  1:1429259/140‑1 (MQ=255)
                                                                                                                          aaTTTTGAGATATCAACATACACCTAGCCCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctg              <  2:1631599/140‑1 (MQ=255)
                                                                                                                          aaTTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctg              <  2:2828412/140‑1 (MQ=255)
                                                                                                                          aaTTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctg              <  2:2491186/140‑1 (MQ=255)
                                                                                                                          aaTTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctg              <  2:2199990/140‑1 (MQ=255)
                                                                                                                          aaTTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctg              <  1:459650/140‑1 (MQ=255)
                                                                                                                          aaTATTGAGATATCAACATACACCTAGACCACTTCATAAGAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctg              <  1:3361607/140‑1 (MQ=255)
                                                                                                                            ttttGAGATATCAACATACACCTAGACCACTTCATACTAGAGCCGCATCCAGAGCACCAAACGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctgct            <  1:2498269/140‑1 (MQ=255)
                                                                                                                            ttttGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAACAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctgct            <  1:3388564/140‑1 (MQ=255)
                                                                                                                            ttttGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAACAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctgct            <  2:103841/140‑1 (MQ=255)
                                                                                                                            ttttGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCACTCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctgct            <  2:3587664/140‑1 (MQ=255)
                                                                                                                            ttttGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctgct            <  2:824467/140‑1 (MQ=255)
                                                                                                                            ttttGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctgct            <  2:108320/140‑1 (MQ=255)
                                                                                                                            ttttGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctgct            <  2:3660613/140‑1 (MQ=255)
                                                                                                                            ttttGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctgct            <  2:3352287/140‑1 (MQ=255)
                                                                                                                            ttttGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctgct            <  1:2499551/140‑1 (MQ=255)
                                                                                                                            ttttGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctgct            <  1:2639084/140‑1 (MQ=255)
                                                                                                                            ttttGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCgctgct            <  2:2655172/140‑1 (MQ=255)
                                                                                                                               tGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCGCTGCtgtg         <  1:2174933/140‑1 (MQ=255)
                                                                                                                                gagaTATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCGCTGCTgtgt        <  2:1139209/140‑1 (MQ=255)
                                                                                                                                      tCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCGGATTGGTTGATTCTTCTTATTTTTTACATTATGTCTTTTGAATTAATTGTCACATTTCGCTCGGTGGTGtgttttgga  >  2:2703736/1‑138 (MQ=255)
                                                                                                                                      tCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCGCTGCTGtgtattgta  <  1:1867088/140‑1 (MQ=255)
                                                                                                                                      tCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCGCTGCTGtgtattgta  >  1:3058241/1‑140 (MQ=255)
                                                                                                                                      tCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCGCTGCTGtgtattgta  <  2:788843/140‑1 (MQ=255)
                                                                                                                                      tCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACACTATGTCTTTTCAATTAAATATCACATTTCACTCGCTGCTGtgtattgta  <  2:784530/140‑1 (MQ=255)
                                                                                                                                      tCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAACCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCGCTGCTGtgtattgta  <  2:887481/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
CTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATTTCACTCGCTGCTGTGTATTGTA  >  NZ_CP034943/1753484‑1753757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: