Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,098,642 C→A 6.5% V297F (GTC→TTC)  EO946_RS10535 ← solute:sodium symporter family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,098,6420CA 6.5% 475.9 / 3.6 210V297F (GTC→TTC) EO946_RS10535solute:sodium symporter family transporter
Reads supporting (aligned to +/- strand):  ref base C (103/84);  new base A (2/19);  total (105/105)
Fisher's exact test for biased strand distribution p-value = 4.93e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

CTCCGAATAAAATAGCGGCGAATACACCGGTAAGCGATGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAGC  >  NZ_CP034943/2098510‑2098781
                                                                                                                                    |                                                                                                                                           
cTCCGAATAAAATAGCGGCGAATACACCGGTAAGCGATGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACtaaataa                                                                                                                                      >  1:3281173/1‑140 (MQ=255)
           aTAGCGGCGAATACACCGGTACGCGATGCCGGCAATAGATCGACGATCAGCCGTGGATATACACTGTCAGCATTTTCATTGCCGCCCCCATATAAATTAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGAt                                                                                                                           <  2:436748/140‑1 (MQ=255)
           aTAGCGGCGAATACACCGGTAAGCGATGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCAATATAAATGAAAGGCAATCACTCCAGGCACGACTAAATAAAAAACGCCGAt                                                                                                                           <  2:3216542/140‑1 (MQ=255)
           aTAGCGGCCAATACACCGTTAAGCTATGCCGGAATTTGATCGACGATAAGCCGTGTTTATACACTGTCAGAATTTTAAATGCCGCCCCCATATAAATCAACGGAAATCACTCCAGGGACGACTAAATAAAAAACGCCGAt                                                                                                                           <  2:1214060/140‑1 (MQ=255)
            tAGCGGCGAATACACCGGTAAGCTATTCCGGCAATAGATCGACGATCAGCCGAGGATATACAATGACAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGAATAAATAAAAAACGCCGATg                                                                                                                          <  2:1771299/140‑1 (MQ=255)
            tAGCGGCGAATACACCGGTAAGCTATGCCGGCAATAGATCGACGATCAGCCGAGTATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGAAATCACTCCAGGGACTACTAAATAAAAAACGCCGATg                                                                                                                          <  2:236514/140‑1 (MQ=255)
               cggcgAATACACCGGTAAGCGATGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGt                                                                                                                       >  2:3333307/1‑140 (MQ=255)
               cggcgAATACACCGGTAAGCGATGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGt                                                                                                                       >  2:2008298/1‑140 (MQ=255)
               cggcgAATACACCGGTAAGCGATGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCAGATGAGt                                                                                                                       >  1:2382095/1‑140 (MQ=255)
                    aaTACACCGGTAAGCGATGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTaaa                                                                                                                  >  1:1489228/1‑140 (MQ=255)
                        cccgggaaaGCGTTGCCGGAAATACATCGCCTATCCGCCGAGTATATACACTGTCAGCATTTTCAAGGCCGCCCCCATATAAATCAAAGGCAATCACACCCGGGACGAATAAATAAAACACGCCGATGAGTTTAAAGaaa                                                                                                              <  1:993666/133‑1 (MQ=255)
                        cacCGGTACGCGAGGCCGGCAATAGACCGACGACCAGCCGCGCATATACAGTGTCACCATTTTCAATCCCGCCCCCATATAACTCACCGCCAATCACTCCCGGCACGACTAAATAAAAAACGCCGATGAGTTTAAAGaaa                                                                                                              <  1:1133197/140‑1 (MQ=255)
                        cacCGGTAAGCGATGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATCAACGGAAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGaaa                                                                                                              <  2:554256/140‑1 (MQ=255)
                          ccGGTAAGCGATGCCGGCAATATATCGACCATAAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATCAAAGGAAATCACCCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAAcc                                                                                                            <  2:1193770/140‑1 (MQ=255)
                           cGGTAAGCGATGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCt                                                                                                           >  1:1938607/1‑140 (MQ=255)
                             gTAAGCGATGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGc                                                                                                         >  2:2566042/1‑140 (MQ=255)
                              tAAGCGATGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCt                                                                                                        >  1:1290716/1‑140 (MQ=255)
                               acgctaTGCCGCAAATCTATCGACCATCAGCCTAGTATATACACTGTAAGATTTTACAATCCCCCCCCAATATAAATTAACGCAAATCACCCCAGGCACCAATAAAAAAAACACGCCGATGAGTTTAAAGAAACCTGCTa                                                                                                       <  1:1192901/135‑1 (MQ=255)
                               aagctttGCCGGAAATCGATCGACGATCAGCCGAGGATATAAACTTTCAGCTTTTTCAATGCCGCCCCAATATAAAGGAACGGAAATCACTCCAGGGACGATTAATTAAAAAACGCCGATGAGTTTAAAGAAACCTGCTa                                                                                                       <  1:2109589/134‑1 (MQ=255)
                                     tGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAagag                                                                                                 >  2:2096741/1‑140 (MQ=255)
                                     tGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAagag                                                                                                 >  1:2600779/1‑140 (MQ=255)
                                       ccGGAAATATAGCGACGATCAGCCGAGTATATACAGTGTCAGCATTTTAAATGCCGCCCCCATATAAATGAAAGGAAATCACACCTGGGACGAAAAAAAAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAgcg                                                                                               <  1:1727014/140‑1 (MQ=255)
                                       ccGGAAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAgcg                                                                                               <  1:735223/140‑1 (MQ=255)
                                                 aTCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTc                                                                                     >  1:2625805/1‑140 (MQ=255)
                                                                   atatACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCg                                                                   <  2:2625805/140‑1 (MQ=255)
                                                                       acacTGTCAGCATTTTCAATGCCGCCCCCATATCAATGAAAGGCAATCAGTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTCAAAGAGCGCCCTTTTGTCCTGCAGCCAAATGTCTGGCTcc                                                               >  1:2139573/1‑140 (MQ=255)
                                                                       acacTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTcc                                                               >  1:2720218/1‑140 (MQ=255)
                                                                       acacTGTCAGCATATTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAAATTCTCGCTcc                                                               >  2:2683340/1‑140 (MQ=255)
                                                                            gTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATg                                                          >  1:3750252/1‑140 (MQ=255)
                                                                            gTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATg                                                          >  1:1055861/1‑140 (MQ=255)
                                                                            gTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATg                                                          >  2:1692025/1‑140 (MQ=255)
                                                                            gTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATg                                                          >  1:2401481/1‑140 (MQ=255)
                                                                            gTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAACTTCTCGCTCCTAATg                                                          >  1:180046/1‑140 (MQ=255)
                                                                             tCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCGAAATTTCTCGCTCCTCATGc                                                         >  2:2275093/1‑140 (MQ=255)
                                                                             tCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGc                                                         >  1:1240887/1‑140 (MQ=255)
                                                                             tCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGc                                                         >  2:1880376/1‑140 (MQ=255)
                                                                             tCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGc                                                         >  1:1543630/1‑140 (MQ=255)
                                                                             tCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGc                                                         >  1:2742400/1‑140 (MQ=255)
                                                                             tCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGc                                                         >  1:1977375/1‑140 (MQ=255)
                                                                             tCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCAGCTAAAAGAGCGCCCGTTGGTCCGTCCGCCACCTTTCTCGCTCCGAATGc                                                         >  2:2989102/1‑140 (MQ=255)
                                                                              cAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGcc                                                        <  2:1938620/140‑1 (MQ=255)
                                                                              cAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGcc                                                        <  2:2418321/140‑1 (MQ=255)
                                                                              cAGAATTTTCATTGCCGCCCCCATATAAATGAAAGGAAATCACTCCCGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGcc                                                        <  2:1489228/140‑1 (MQ=255)
                                                                               aGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGccc                                                       >  2:722751/1‑140 (MQ=255)
                                                                               aGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGccc                                                       >  1:2563163/1‑140 (MQ=255)
                                                                                     ttCAATGCCGCCCCCATATCAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGTAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCCAATTTCTCGCTCCTAATGCCCGCTGGa                                                 >  2:3206927/1‑140 (MQ=255)
                                                                                          tGCCGCCCCCATATAAATGAAAGGAAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATc                                            <  2:2154647/140‑1 (MQ=255)
                                                                                          tGCCGCCCCCATATAAATCAAAGGCAATCCCTCCAGGGACGACTAAATAAAACACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATc                                            <  1:2472903/140‑1 (MQ=255)
                                                                                          ggccgccCCCATATAAATCAAAGGAAATCACTCCAGGGACCAATAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATc                                            <  1:3097712/139‑1 (MQ=255)
                                                                                          ggccgccCCCATATAAATCAAAGCCAATCACTCCAGCGACGACTAAATAAACAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATc                                            <  1:2889651/139‑1 (MQ=255)
                                                                                             cgccCCCATATAAATTAAAGGCAATCACGCCCGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCt                                         <  2:605596/140‑1 (MQ=255)
                                                                                             cgccCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCt                                         <  2:2401481/140‑1 (MQ=255)
                                                                                             cgccCCCATATAAATCAACGGCAATCCCACCCGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCt                                         <  1:3836423/140‑1 (MQ=255)
                                                                                             cgccCCCATATAAATCAAAGGCAATCACTCCCGGGACGACTAAATAAAACACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCt                                         <  1:359400/140‑1 (MQ=255)
                                                                                             cgccCCCATAAAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAGCCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCt                                         <  2:1419155/140‑1 (MQ=255)
                                                                                             cgccCCCAAATAAATCAAAGGCAATCACTCCAGGGACTACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCt                                         <  1:649893/140‑1 (MQ=255)
                                                                                              gccCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCtt                                        >  1:2035010/1‑140 (MQ=255)
                                                                                               cccccATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTg                                       >  2:2750045/1‑140 (MQ=255)
                                                                                                     tataAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAAc                                 >  2:3299104/1‑140 (MQ=255)
                                                                                                       taAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAg                               <  2:2720218/140‑1 (MQ=255)
                                                                                                       taAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAg                               <  2:2940594/140‑1 (MQ=255)
                                                                                                         aaTTAAAGGAAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGcc                             <  1:3535878/140‑1 (MQ=255)
                                                                                                         aaTTAAAGGAAATAACTCCAGGGACTAATAAATAAAACACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGcc                             <  1:2908766/140‑1 (MQ=255)
                                                                                                           tGAACGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCAAAAAGACCGCCCTTTTGTCCATCAGCCAAATATCTCGCTCCTAATGCCCGCTGGACGATCGCTGGGGTAACAGCCCa                           >  2:3444749/1‑140 (MQ=255)
                                                                                                           tGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCa                           >  2:30272/1‑140 (MQ=255)
                                                                                                              aaGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATa                        >  2:3683918/1‑140 (MQ=255)
                                                                                                              aaGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATa                        >  2:1111310/1‑140 (MQ=255)
                                                                                                               aGGCAATCAGTCCAGGGACGAAGAACTAAAACACGCAGATGAGTTTAAAGAAACCTGCTATAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCGAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCacgaa                       >  2:2553414/1‑136 (MQ=255)
                                                                                                                   attCAGTCCTGGGACGTCTAAATAAAAATCGAATATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTGCTTCTTGAAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGTTTAACAGCACATTAAAaga                   <  2:2314213/138‑1 (MQ=255)
                                                                                                                   aaTCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAaga                   <  2:3757798/140‑1 (MQ=255)
                                                                                                                   aaTCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAaga                   <  2:2781576/140‑1 (MQ=255)
                                                                                                                    aTCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAagag                  >  1:2223229/1‑140 (MQ=255)
                                                                                                                    aTCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGAGACCTTTTGTCATTCAGACAAATTTCTCGCTCATAATGCCCGCTGGACGATCGCTTGGTTAAAAGCCCAATAAAagat                  >  1:350830/1‑139 (MQ=255)
                                                                                                                      cACTCCAGGGACGAATAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGat                <  2:3129943/140‑1 (MQ=255)
                                                                                                                      cACGCCAGGGAAGAATAAATAAAACACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGat                <  1:2958341/140‑1 (MQ=255)
                                                                                                                          ccAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGattatt            <  1:2069656/140‑1 (MQ=255)
                                                                                                                          ccAGGGACGACAAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGattatt            <  2:2259916/140‑1 (MQ=255)
                                                                                                                          ccAGGCAAGACAAAACAAAACACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGattatt            <  1:2499066/140‑1 (MQ=255)
                                                                                                                           cAGGTACTACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTc           <  2:1229524/140‑1 (MQ=255)
                                                                                                                           cAGGGACGACTACATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCAAAATATCTCGCTCCTAATGCCCGCTGGAAGATCGCTTGGTTAACAGACCAATAAAATAGATTATTc           >  1:2297155/1‑140 (MQ=255)
                                                                                                                           cAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTc           <  2:2035010/140‑1 (MQ=255)
                                                                                                                           cAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTc           >  1:332167/1‑140 (MQ=255)
                                                                                                                           cAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTc           >  1:1671563/1‑140 (MQ=255)
                                                                                                                           cAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTc           >  1:450328/1‑140 (MQ=255)
                                                                                                                                  gacTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTaa      >  1:781221/1‑138 (MQ=255)
                                                                                                                                  cacTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTaa      <  2:781221/137‑1 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAAtcg   >  1:2569034/1‑138 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAg   >  2:2927986/1‑140 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAg   >  1:1368465/1‑140 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAg   >  1:3178345/1‑140 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAg   >  2:3472682/1‑140 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAg   >  2:1668445/1‑140 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAg   >  1:3580496/1‑140 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAg   >  1:1593107/1‑140 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAg   >  1:915758/1‑140 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAg   >  2:673335/1‑140 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAg   <  1:89055/140‑1 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAg   >  1:608989/1‑140 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCCTATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAg   >  2:1824328/1‑140 (MQ=255)
                                                                                                                                   acTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTACTCGCTCCTAATTCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAATAGAGATTATTCACTAATa  >  2:3841833/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                           
CTCCGAATAAAATAGCGGCGAATACACCGGTAAGCGATGCCGGCAATAGATCGACGATCAGCCGAGGATATACACTGTCAGCATTTTCAATGCCGCCCCCATATAAATGAAAGGCAATCACTCCAGGGACGACTAAATAAAAAACGCCGATGAGTTTAAAGAAACCTGCTAAAAGAGCGCCCTTTTGTCCTTCAGCCAAATTTCTCGCTCCTAATGCCCGCTGGACGATCGCTTGGTTAACAGCCCAATAAAAGAGATTATTCACTAATAGC  >  NZ_CP034943/2098510‑2098781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: