BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,214,528	G→T	6.2%	Q57K (CAA→AAA)	nth ←	endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,214,528	0	G	T	6.2%	674.9 / 4.6	280	Q57K (CAA→AAA)	nth	endonuclease III
Reads supporting (aligned to +/- strand): ref base G (59/193); new base T (20/7); total (79/201)
Fisher's exact test for biased strand distribution p-value = 2.50e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

CCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGA  >  NZ_CP034943/2214389‑2214667

ccgccgTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTg                                                                                                                                             <  1:3520508/140‑1 (MQ=255)

   ccgTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGaaa                                                                                                                                          >  2:3342837/1‑140 (MQ=255)

   ccgTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGaaa                                                                                                                                          <  2:1262489/140‑1 (MQ=255)

      tAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTTTAATTTTTAaataa                                                                                                                                       >  1:1110781/1‑140 (MQ=255)

      tAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaataa                                                                                                                                       >  2:2127428/1‑140 (MQ=255)

             cAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGTAATAAttttttt                                                                                                                                >  2:1662227/1‑139 (MQ=255)

              aataatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTTAATAAAGTTTTgg                                                                                                                               >  2:2633684/1‑140 (MQ=255)

                taatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAGGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGTAATAATTTTTTGGTc                                                                                                                             >  2:1703299/1‑140 (MQ=255)

                taatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATTTTTTTGta                                                                                                                             >  2:1370376/1‑139 (MQ=255)

                taatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTTGTc                                                                                                                             >  2:228238/1‑140 (MQ=255)

                taatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCAAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAAAAATGTTTTGGTc                                                                                                                             >  2:2378747/1‑140 (MQ=255)

                 actCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCa                                                                                                                            <  1:3000558/138‑1 (MQ=255)

                 aatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATCTCCTGCTGAAGCTCGTCCAGCGAAACCGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCa                                                                                                                            <  1:7335/140‑1 (MQ=255)

                 aatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCa                                                                                                                            <  1:382915/140‑1 (MQ=255)

                   ttaTTTTGCTCAGTTTTTGTATGTTTTTGGCTTTATTACGATACAAACCGATTGATTTTATATCCTTCTGAAGCTATTCCAGCGAAACAGCCAAATAGTATTCCGGCCGTTTATATTTTTTAAATAATGTTTTTGTCAct                                                                                                                          <  2:1198286/138‑1 (MQ=255)

                   tctttttGATCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAAGATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:2821750/137‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTTGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:3485946/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:1001294/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:167772/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  2:2081516/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:2358658/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:3705760/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:2748239/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:2659410/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTGTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTGTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:3106679/140‑1 (MQ=255)

                       tttGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGt                                                                                                                      >  2:2833245/1‑140 (MQ=255)

                           cTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTGACTCTGTTTaa                                                                                                                  >  2:2396823/1‑139 (MQ=255)

                           cTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTAc                                                                                                                  <  2:1065602/140‑1 (MQ=255)

                              agtatgtGGATGTGTTTCGCTGTATTACGATACCAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:2075167/134‑1 (MQ=255)

                              aGTTTTTGGATTTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGACGTTGATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  2:3426806/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTTAAGCTCTTCCAGCGAAACAGCCAAATATTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:663769/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:2615106/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:1610770/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCGTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATCTTTTGGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:1416930/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACAGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:2469670/140‑1 (MQ=255)

                                tttttGGTTGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGGTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  2:1800738/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCTAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  2:530628/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:3408633/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:3155868/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  2:2749729/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:2811796/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:1302740/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  2:3741459/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:601319/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGAGAAACAGCCAATTATTCTTCAGTCGTTTTATATTTTTTAAATAATGTTTTGGTAAGTATGTTTatttgtt                                                                                                             <  1:2017613/140‑7 (MQ=255)

                                    tGGATGTTTGTCGCTTTATTACGATACAAACCGATTGATTTAATAGCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATc                                                                                                         <  2:1320115/140‑1 (MQ=255)

                                     ggATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCt                                                                                                        >  2:377958/1‑140 (MQ=255)

                                        tGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaata                                                                                                                                        <  1:2448248/105‑1 (MQ=255)

                                            tttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAAAAATTTTTTGGTCACTCTGTTTACAACTGCATATTTACaat                                                                                                 >  2:2597451/1‑138 (MQ=255)

                                                    tattaCGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGTAATAATGTTTTGGTGACTCTGTTTAAAATTGCATCTTTACATTGCGCAGAt                                                                                         >  1:1879634/1‑140 (MQ=255)

                                                    tattaCGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTTAATAAATTTTTTTTGACTGTGTTTTGAATTTCATCTGTAGATTGCGCAGAt                                                                                         >  1:3335334/1‑140 (MQ=255)

                                                            tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAAAAAAGGTTTTGTCACTCTGTTTACAAGTGCATCTGTAAATTGCGCACATAAAGAGAc                                                                                 >  2:499714/1‑140 (MQ=255)

                                                            tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGAc                                                                                 >  2:849168/1‑140 (MQ=255)

                                                              cAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAAAAAAGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTAAAATTCGCACATAAAGCGACTg                                                                               >  1:3588492/1‑140 (MQ=255)

                                                                acccGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCAACTGTACATTGCGCAGATAAAGCGACTGCt                                                                             <  2:2485864/138‑1 (MQ=255)

                                                                     attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCAc                                                                        <  2:1856973/140‑1 (MQ=255)

                                                                      ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATTTTTTTGTGACGCTGTTTTAAACTGCATCTGTAAATTTGGCACATAAAGCGACATCAACCAca                                                                       >  1:1662566/1‑139 (MQ=255)

                                                                      ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATGTTTTTGTGACTCTGTTTAAAATTGCATCTGTAAATTTCGCAGATAAAGCCACTGGTACCACt                                                                       >  1:2407357/1‑140 (MQ=255)

                                                                      ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTGTGTCACACTGGTTAAAAGGGCATCTGTACCTGGCGCAGATAAAGCGAGTGCTTccccg                                                                       >  1:930650/1‑137 (MQ=255)

                                                                        gattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTaa                                                                     <  2:2465051/140‑1 (MQ=255)

                                                                                   cttctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTGATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                          <  2:3079026/137‑1 (MQ=255)

                                                                                   ctgcttAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTTTTTACAAGTGCATCTGTACTTTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                          <  2:3455006/140‑1 (MQ=255)

                                                                                   ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                          >  2:636364/1‑140 (MQ=255)

                                                                                   ctgcataaGCTCATCCAGCGAAAAAGCAAAATTGTCGACCGGCCGTCTATATTTTTGAAATAAAGTGTGGGTCACTCTGTTTACAAGTGCATCTGTACCTTGCGCAGCTAAAGCGACTTCTACCACTAATTCAAAAGGAt                                                          <  2:3221793/134‑1 (MQ=255)

                                                                                    tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAAAAATGTTTTGGTCCCTCAGATTACAAGTGCATCTGTACCTTGCGCACATAAACCGACTGCTACCACTAATTCAAAAGGAtg                                                         >  2:2247650/1‑139 (MQ=255)

                                                                                    tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGTAATAATGTTTTGGTGACTCTGTTTAGAAATGCCTCTGTACATTGGGCAGATAAAGCCACTGCTACCACGAAATCAAAAGGAtt                                                         >  1:2201740/1‑140 (MQ=255)

                                                                                    tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTATTTTTACAATTGCATCTGTACATTTAGGAGATAAAGCCACTGCTACCACTAATTCAAAAGGaat                                                         >  2:3306700/1‑138 (MQ=255)

                                                                                    tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGGCACTCTGTTTACAAGTGCATCTGGACATTGCGCAGATAAATCGACGGCTACCACTAATTAAAAAGGAtt                                                         >  2:770245/1‑140 (MQ=255)

                                                                                    tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTGTAATAATTTTTTTGTGACTGTGTTTTCAAGTGCATCTGTAAATTTCGGAGATAAAGCCACTGCTACCACTAATTCAAAAGGttt                                                         >  1:3578474/1‑140 (MQ=255)

                                                                                    tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTTAATAATGTTTTTGTCACACTGTTTTCAAGTTCATATGTACATTTCGCACATAAATCGACAGCTACCACTAAATAAAAAGGAtt                                                         >  1:1950908/1‑140 (MQ=255)

                                                                                    tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGTAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACCTTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAtt                                                         >  2:1574266/1‑140 (MQ=255)

                                                                                       tgAAGCTCTTCCCGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGa                                                      <  1:1752617/140‑1 (MQ=255)

                                                                                        gAAGCTCTTCCCGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGaa                                                     <  1:285232/140‑1 (MQ=255)

                                                                                        gAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGaa                                                     <  1:1858632/140‑1 (MQ=255)

                                                                                            ctctTCGAGCGAAACAGCCAAAGAGTCTTCGGGCCGTTTATATGGTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCTTCTGTACATTGCGCAGATAACGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  2:2427749/140‑1 (MQ=255)

                                                                                            ctctTCCCGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACCCTGTTTCCACGTGCATCTGTACATTGCGCAGCTCAAGCGACGGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  2:21552/140‑1 (MQ=255)

                                                                                            ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCTTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  2:3067812/140‑1 (MQ=255)

                                                                                            ctctTCCAGCGAAACAGCAAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  1:798982/140‑1 (MQ=255)

                                                                                            ctctTCAAGCGAAACAGCCAAATAGTATTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAATTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  1:1566236/140‑1 (MQ=255)

                                                                                            ctagtCCAGCCAAACAGCCAAATAGTATTCCGGCGGTTTATATTTTTGAAATAATTTTTTGGTCACTCTGTTTACATGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  1:176901/136‑1 (MQ=255)

                                                                                              ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATTTTTTGGTCACTCTGTTTACAAGTGCAACTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                               <  1:445387/140‑1 (MQ=255)

                                                                                              ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                               <  1:377958/140‑1 (MQ=255)

                                                                                                tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTTTTTTTTTGTAAAAATTTTTTTGTCACTCTGTTTACAAGTGCATCTGTAAATTTCGCAGATAAAAGGACTGCTACCACTAAATGAAAAGGATTGGAATGGACCAg                                             >  1:3098036/1‑140 (MQ=255)

                                                                                                  catcGAAACAGCAAAATAGTCTTGCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGTAATGAACCAGtt                                           <  2:113181/137‑1 (MQ=255)

                                                                                                  cAGCGAAACAGCCAAATAGTCTTCCGGCCTTTGATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                           <  1:1589234/140‑1 (MQ=255)

                                                                                                  cAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                           <  1:2185753/140‑1 (MQ=255)

                                                                                                  cAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                           <  1:228238/140‑1 (MQ=255)

                                                                                                  cAGCGAAACAGCCAAATAGTCTTCCGGCCGGTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                           <  2:1090992/140‑1 (MQ=255)

                                                                                                  cAGCGAAACAGCAAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                           <  1:1370376/140‑1 (MQ=255)

                                                                                                     cGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAAAGTTTTGGTCACTCTGTTTTCAAGTGCATCTGTCACTTGCGCAGATAAAGGGTATGATAACACTAATTAAAAAGGTTATGAATGGACCAGTTaac                                        >  2:1932149/1‑140 (MQ=255)

                                                                                                      gAAACAGCCAATTAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTcaca                                       <  2:2988571/140‑1 (MQ=255)

                                                                                                      gAAACAGCCAAATAGTCTTCCGGCCGTTTATAGTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTcaca                                       <  2:304683/140‑1 (MQ=255)

                                                                                                                             ccGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc                <  2:3588492/140‑1 (MQ=255)

                                                                                                                             ccGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc                <  1:499714/140‑1 (MQ=255)

                                                                                                                                 ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt            <  2:162278/140‑1 (MQ=255)

                                                                                                                                 ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt            <  2:738600/140‑1 (MQ=255)

                                                                                                                                 ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCGGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt            <  2:570770/140‑1 (MQ=255)

                                                                                                                                          tGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAg   >  1:343940/1‑140 (MQ=255)

                                                                                                                                           gAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGa  <  1:1655027/140‑1 (MQ=255)

                                                                                                                                           gAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGa  <  1:1852537/140‑1 (MQ=255)

CCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGA  >  NZ_CP034943/2214389‑2214667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`