Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,502,212 T→G 11.7% N284H (AAC→CAC)  hrcA ← heat‑inducible transcriptional repressor HrcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,502,2120TG11.7% 332.7 / 3.4 169N284H (AAC→CAC) hrcAheat‑inducible transcriptional repressor HrcA
Reads supporting (aligned to +/- strand):  ref base T (88/52);  new base G (27/2);  total (115/54)
Fisher's exact test for biased strand distribution p-value = 8.88e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

CACATGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGAT  >  NZ_CP034943/2502075‑2502350
                                                                                                                                         |                                                                                                                                          
cacaTGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGtttt                                                                                                                                          <  2:3379702/140‑1 (MQ=255)
  caTGCTGAAGCAGGCTGACAACCCTGGACTAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTtttct                                                                                                                                        >  1:1765642/1‑140 (MQ=255)
  caTGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTtttct                                                                                                                                        >  2:1464695/1‑140 (MQ=255)
  caTGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTtttct                                                                                                                                        >  1:2953582/1‑140 (MQ=255)
  caTGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTtttct                                                                                                                                        >  2:1784761/1‑140 (MQ=255)
  caTGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTtttct                                                                                                                                        >  1:1328692/1‑140 (MQ=255)
  caTGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTgttct                                                                                                                                        >  1:2502834/1‑140 (MQ=255)
  caTGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTgttcg                                                                                                                                        >  2:3333610/1‑139 (MQ=255)
         aaGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCgc                                                                                                                                 >  1:2349798/1‑139 (MQ=255)
         aaGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGa                                                                                                                                 >  1:3637321/1‑140 (MQ=255)
              ggCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTa                                                                                                                            >  2:3237441/1‑140 (MQ=255)
              ggCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTa                                                                                                                            >  2:2494580/1‑140 (MQ=255)
              ggCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTa                                                                                                                            >  2:1745624/1‑140 (MQ=255)
               gCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTaa                                                                                                                           >  2:3418722/1‑140 (MQ=255)
                cTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAAt                                                                                                                          <  1:1191519/140‑1 (MQ=255)
                  gACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCg                                                                                                                        <  1:334877/140‑1 (MQ=255)
                   aCAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGGTTTTTATCGa                                                                                                                       >  2:3514722/1‑140 (MQ=255)
                      aCCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAAt                                                                                                                    >  2:1035890/1‑140 (MQ=255)
                         cTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATccc                                                                                                                 <  2:654059/140‑1 (MQ=255)
                          tGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATcccc                                                                                                                >  1:2315147/1‑140 (MQ=255)
                          tGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCCCTTTTAATCGAAAAcccc                                                                                                                >  1:322858/1‑140 (MQ=255)
                          tGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTTATCGAAATcccc                                                                                                                >  1:1873605/1‑140 (MQ=255)
                           ggAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCg                                                                                                               <  1:1565959/140‑1 (MQ=255)
                            gAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCGATTTTAATAGAAATCCCCgt                                                                                                              >  1:1387913/1‑140 (MQ=255)
                                 atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGACTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCGTCATAGTCGTTGTGGTTCCCGATTTTAATCGAAATCCCCGggtgtg                                                                                                         >  1:1709548/1‑140 (MQ=255)
                                 atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCGATTTTAATCGAAAAACCCggggggg                                                                                                         >  1:1984576/1‑134 (MQ=255)
                                  ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCCATTTTAAACGAAAAAACCGTGTGTgg                                                                                                        >  1:2457639/1‑140 (MQ=255)
                                  ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCgtgggggg                                                                                                        >  1:3811592/1‑135 (MQ=255)
                                  ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTgg                                                                                                        >  2:1811471/1‑140 (MQ=255)
                                  ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAAACCCCCTGTGTgg                                                                                                        >  1:491339/1‑140 (MQ=255)
                                  ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGGTTTCGTTGCCGATTGTCATCGAAATCCCCGTGTGTgg                                                                                                        >  2:1981102/1‑140 (MQ=255)
                                  ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCAGCTCTTCATAGTCGGTGTCGTTCCCGAGTTTAATCGAAATCGCCGGGGGTgg                                                                                                        >  2:3083420/1‑140 (MQ=255)
                                      tGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCgt                                                                                                              <  2:725728/130‑1 (MQ=255)
                                      tGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAAACCCCGGGTGTGGGGAt                                                                                                    >  1:3388091/1‑140 (MQ=255)
                                       gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGAtt                                                                                                   >  2:734555/1‑140 (MQ=255)
                                       gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGAtt                                                                                                   >  2:3404842/1‑140 (MQ=255)
                                           ggcGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGAGCAACGGAATCAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGCTTTTAATCGAAAAGCCCGTGGGGGGGGATTGCAc                                                                                               >  2:2212579/3‑140 (MQ=255)
                                           gTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATAGAAATACCCGTGTGTGGGGATTGTAc                                                                                               >  2:1243602/1‑140 (MQ=255)
                                            tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                              <  2:3463023/140‑1 (MQ=255)
                                            tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                              <  2:3022522/140‑1 (MQ=255)
                                            tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                              <  2:1328692/140‑1 (MQ=255)
                                                    atgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAAACCCCGTGTGTGGGGGTTGTACGAGGTTTaa                                                                                      >  1:3056537/1‑140 (MQ=255)
                                                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAAACCCCGTGTGTGGGGATTGTAAGTGCTTTaaa                                                                                     >  1:2137808/1‑140 (MQ=255)
                                                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATACCCGTGTGTGGGGATTTAACGAGCTTTaaa                                                                                     >  1:2904196/1‑140 (MQ=255)
                                                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAAAACCCGTGTGTGGGGATTGAAAGAGCTTTaaa                                                                                     >  1:1079097/1‑140 (MQ=255)
                                                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTGAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTaaa                                                                                     <  2:2338430/140‑1 (MQ=255)
                                                         cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCCATTTTAATCGAAATCCCCGGGGGTGGGGTTTTTACGAGCTTTTAAACAt                                                                                 >  1:3857671/1‑140 (MQ=255)
                                                         cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAAAACCCGTGTGTGGGGATTGAACGAGCTTTAAAACAt                                                                                 >  1:3857414/1‑140 (MQ=255)
                                                            aaTTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATAGAAATCCCCGTGGGTTGGGATTGAACGAGCTTTAAAACATGCt                                                                              >  2:2512156/1‑140 (MQ=255)
                                                                   ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCAGATTTTAAACGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTAttt                                                                       >  2:1021932/1‑140 (MQ=255)
                                                                   ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAAACCCCGGGTGTGGGGCTTGAACGAGCTTTAAAACATCCTGTTCttt                                                                       >  2:3126514/1‑140 (MQ=255)
                                                                   ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATAGAAATCCCCGTGTGTGGGGATTTAAAGAGCTTTTAAACATGCTGTTGttt                                                                       >  2:1591286/1‑140 (MQ=255)
                                                                   ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATAGAAATACCCGTGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCttt                                                                       >  2:893918/1‑140 (MQ=255)
                                                                     gATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttttt                                                                     <  2:3637321/140‑1 (MQ=255)
                                                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATACCCGGGTGTGGGGATTTAACGAGCTTTAAAAAATACTGTTAttttta                                                                    >  1:2659407/1‑139 (MQ=255)
                                                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGCTTGAACGAGCTTTAAAACATCCTGTTAttttta                                                                    >  2:79565/1‑139 (MQ=255)
                                                                        cTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCaa                                                                  <  2:2173857/140‑1 (MQ=255)
                                                                          gCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAAtt                                                                <  2:754215/140‑1 (MQ=255)
                                                                           cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGAATTTAAAAGAAATACCCGTGTGTGGGGCTTGAACGAGGGTTAAAAAATCCAGTTTTTTTTGAATTa                                                               >  1:3405515/1‑140 (MQ=255)
                                                                           cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGGTTTCCATGTCTTCATAGTCGTGTTAGTTCCCGATTTTAATCGAAATCCCCGTGGGGGGGGATTGACCGAGCGTTAAAACATCCGGTTCTTTTTCCATTa                                                               >  2:391965/1‑140 (MQ=255)
                                                                              gtGATCAACGTAATAAGACGGTTTAATCAGACTTCAGTTTTCCATCTCTTTATTGTCTTTTTATTTCCCGATTTTAATCGAAATCCCGGTGTGTGGGTTTTGAACGAGCTTTAAAACATCCTTTTCTTTTTAAATTAATg                                                            <  1:3137353/139‑1 (MQ=255)
                                                                                 aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATACCCGGGGTTGGGGATTGTACGAGCTGTAAAACATCCTTTTCTTTTTAAATTAATgaga                                                         >  1:960434/1‑140 (MQ=255)
                                                                                     aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAAAGTGCTTTAAAACATGCTGTTCTTTTTCAATTAATGAGAGAAg                                                     >  2:3690592/1‑140 (MQ=255)
                                                                                     aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAg                                                     >  2:39821/1‑140 (MQ=255)
                                                                                      cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGc                                                    >  2:2707997/1‑140 (MQ=255)
                                                                                      cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGGGCTTTAAAACATCCTGTTCTTTTTCAATTAATGTGAGAAGc                                                    >  2:2945344/1‑140 (MQ=255)
                                                                                      cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTATTTTTCAATTAATGATAGAAGc                                                    >  2:1917320/1‑140 (MQ=255)
                                                                                      cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCGTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGc                                                    >  2:1724798/1‑140 (MQ=255)
                                                                                          aTAAGAAGCCGTAATCAGACTGCATTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAATGAGAGAAGCGAcc                                                <  1:3004511/140‑1 (MQ=255)
                                                                                          aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                <  1:1035890/140‑1 (MQ=255)
                                                                                          aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                <  1:3522181/140‑1 (MQ=255)
                                                                                          aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                <  1:743966/140‑1 (MQ=255)
                                                                                          aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                <  1:470607/140‑1 (MQ=255)
                                                                                          aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGATTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                <  1:1216974/140‑1 (MQ=255)
                                                                                             acaggCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGaa                                             <  2:1838020/136‑1 (MQ=255)
                                                                                                agCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCCATTTTAAACGAAAACCCCCGGTGTGGGGATAGAACGAACTTTATAAAAACATGTTCTTTTTAAATTAATTAGAGAAGAGAACGGAcgc                                          >  1:1867015/1‑138 (MQ=255)
                                                                                                 gCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGGGGGGGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAATCGACCGAActct                                         >  2:693629/1‑140 (MQ=255)
                                                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGATTGAACGCGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACACTgg                                       >  2:2280431/1‑140 (MQ=255)
                                                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGGGTGGGGATTGAACGAGCTTTAAAAAATCCGGTTCTTTTTAAATTAATTAGAGAAGCGACCGAACTCggg                                       >  2:3147078/1‑140 (MQ=255)
                                                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATAGAAAAACCCGGGGGGGGGGGTTGAACGAGCTTTAAAAAAAACTGTTCTTTTTAAATTAATTATAGAAGCGACCGAACACTgg                                       >  1:1604378/1‑140 (MQ=255)
                                                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGATTTTAATCGAAAAACCCCTGTGGGGGGATTGAACGAGCGTTAAAAAATACTGTTATTTTTCAATTAATGAGAGAAGCGACCGACCTCTgg                                       >  1:2902983/1‑140 (MQ=255)
                                                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTGCCGATTTTAATCGAAATCCCCGGGTGTGGGGCTTTAACGCGCTTTTAAACATCCGGTTGTTTTTGAATTAATGAGGTAAGCGACCCAAcgcggg                                       >  1:1420062/1‑135 (MQ=255)
                                                                                                    gTAATCAGACTGGAGTTTTCCATCTCTTCATAGGCGTGTTCTTGCCCGATTGTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTGAATTACTGAGAGAAGCGACGGAACTCTGGt                                      <  2:3104104/140‑1 (MQ=255)
                                                                                                    gTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGt                                      <  1:2189365/140‑1 (MQ=255)
                                                                                                    gTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGt                                      <  1:1745623/140‑1 (MQ=255)
                                                                                                      aaTCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGa                                    <  2:116228/140‑1 (MQ=255)
                                                                                                            aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCAt                              >  2:2122293/1‑140 (MQ=255)
                                                                                                             cTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATg                             <  2:2413895/140‑1 (MQ=255)
                                                                                                             aggCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATg                             <  1:548753/138‑1 (MQ=255)
                                                                                                                cAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTTATAGAAATACCCGGGTGTGGGGATTTTACGAGCGTTAAAACATCCTGTTGTTTTTCAATTAATGAGAGAAGCGACCGAACGCGGGTGGTTTCATggta                          >  1:469414/1‑138 (MQ=255)
                                                                                                                cAGTTTTCCATCTCTTCATAGTCGTGTTGTTTGCCGATTTTAATCGAAATCCCCGGGGGTGGGGCTTGAACGAGCGTTAAAACATCCTGTTGTTTTTAAATTAATTAGAGAATCGACCGCACGCTGGTGATATCATGGaa                          >  1:2626582/1‑140 (MQ=255)
                                                                                                                cAGTTTTCCATCTCTTCATAGTCGTGTTCTTTACCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAAAGAGCTTTAAAAAATACTGTTCTTTTTAAATTAAATAGAGAAACGACCGAACTCTGGTGATATCATGGaa                          >  2:1726631/1‑140 (MQ=255)
                                                                                                                       ccATCTGTTCATTGTCTTTTTCTTTCCCTATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                   <  1:2424344/140‑1 (MQ=255)
                                                                                                                       ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                   <  1:1839922/140‑1 (MQ=255)
                                                                                                                          tctcTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAATCGACCGAACGCTGGTGATATAATGGAACTCCGGCtct                >  2:548753/1‑138 (MQ=255)
                                                                                                                             ctTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTTAACGAGCTTTAAAACATCCAGTTCTTTTTCAATTAATTAGCGAATCGACCGAACTCTGGTGATATAATGGAACTCCGGCTGGTTc             >  2:3592073/1‑140 (MQ=255)
                                                                                                                              ttCTTAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTTTGGGGATTTAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCa            <  2:3669612/140‑1 (MQ=255)
                                                                                                                              ttCATAGTCGTTTTCTTTCCCTATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCa            <  2:3803425/140‑1 (MQ=255)
                                                                                                                              ttCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCa            <  2:3261456/140‑1 (MQ=255)
                                                                                                                                        ttttCTTTCCCGATTTTAAACGAAAACCCCGTTTGGGGGGATTTAACGAGCTTTAAAACATCCCGTTCTTTTTAAATTAATGCTCGAAGCGACCCAACGCGGGTGCAATCATGGAACACCCGCAGGGTCAGCATATAGAt  >  2:3748000/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CACATGCTGAAGCAGGCTGACAACCCTGGAATAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGAT  >  NZ_CP034943/2502075‑2502350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: