Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,900,793 C→A 6.5% P118P (CCC→CCA EO946_RS14945 → extracellular solute‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,900,7930CA 6.5% 658.5 / 3.3 254P118P (CCC→CCAEO946_RS14945extracellular solute‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (130/97);  new base A (2/25);  total (132/122)
Fisher's exact test for biased strand distribution p-value = 4.87e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

ACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACCCGCCGGAAACCATGGATGA  >  NZ_CP034943/2900654‑2900927
                                                                                                                                           |                                                                                                                                      
aCTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATccc                                                                                                                                        <  2:520299/140‑1 (MQ=255)
  tATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCaa                                                                                                                                      >  2:1895136/1‑140 (MQ=255)
    tGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAAtt                                                                                                                                    >  1:1146656/1‑140 (MQ=255)
          tatGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACa                                                                                                                              >  2:1075372/1‑140 (MQ=255)
                   tgtCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCa                                                                                                                     >  1:1443333/1‑140 (MQ=255)
                    gtCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCaa                                                                                                                    >  1:408787/1‑140 (MQ=255)
                              cAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGc                                                                                                          >  1:1214009/1‑140 (MQ=255)
                                 ccATTGATTTGCCTGAACTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGTAGAATATACAGCGTATCTCAGAGATAGGGGATGGACCCAAGATCTCTATCCAAATTTAAACAGCATCCTAAAAAATAAACGCGGc                                                                                                       <  1:3643338/140‑1 (MQ=255)
                                 ccAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGc                                                                                                       <  2:1214009/140‑1 (MQ=255)
                                  cattgttttGCCTGAATTTTTCTATACAAATGGGTGGTTAAAAATTAGGTATGGATAATATACAGGGGATCTCATAGATTTGTGATGGACCAAAGATCTCGATCCCAATTTAAACCGCATCCTAAAAAATAAAAGCGGCa                                                                                                      <  1:2174032/134‑1 (MQ=255)
                                  cattGATTTCCCTTACCTTTTCTATACAAATGGCTGGGGAAAATTTTGTTATGTATAATATACAGCGTATATCAGATATTTGGTATTGACCAAATATCTCTATCCAAATTTAAAAACCAGCCTCAAAAATAAAAGCGGCa                                                                                                      <  1:2919689/137‑1 (MQ=255)
                                  cactGATTTGCTTGACCTTTTCGATACACATGGATGGGTAAAATTTATTTATGGATAATATACAGCGGATCTCAGATTTATGTGATTTACCCAAGTTCTCGATCAAAATTTATACAGCATCCTAAAAAATAAAAGCGTCa                                                                                                      <  2:2164004/137‑1 (MQ=255)
                                  caattttttGCCTGACATTTTCTATACAATTGGCTGGGTTAAATTTTGGTTTGTTTAATATACTGCTTATCTAAGAGTTTTGGGTTGTACAAAATATCTGTATCCAAATTTAAACAGCTTACTCAAAAATAAAAGCGGCa                                                                                                      <  1:2036771/134‑1 (MQ=255)
                                  cAATTATTTGCCTGACCTTTTCGATACACATGGGTGGGGAAAAATTAGGTATGGACAATATACAGCGTATCTCAGAGATAGGGTTTGGACCCAAGATCTCGATCCCAATTTAAACACCATCATCAAAAATAAAAGCGGCa                                                                                                      <  1:2652587/140‑1 (MQ=255)
                                  cAATGTTTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCAAAGATCTCGATCCAAATTTAAACAGCATCCTCAAAAATAAACGCGGCa                                                                                                      <  2:2508219/140‑1 (MQ=255)
                                  cAATGATTTGCGTGACATTTTCTATAAAAATGGCTGGGGAAAAATTAGTTATGGAGAATATAAAGCGGATCTCAGAGATTTGGGATGGACCCAAGATCTCGATCCAAATTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                                                                                      <  2:2343363/140‑1 (MQ=255)
                                  cAATGATTTGCCTGACCTTTTCGATACACATGGCTGGTTAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                                                                                      <  2:2067395/140‑1 (MQ=255)
                                  cAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGTAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                                                                                      <  2:2695374/140‑1 (MQ=255)
                                  cAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGTTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCACAAAAATAAAAGCGGCa                                                                                                      <  2:2595329/140‑1 (MQ=255)
                                  cAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                                                                                      <  2:3592291/140‑1 (MQ=255)
                                  aaaTTATTTGCCTTACCTTTTCTATAAAATTGGCTGGGTAAAAATTATTTTTGGACATTATACAGCGTATATCATTTATATGGGTTGGACCCAAGATCTCTATCCCAATTTAAACAGAATCCTCAAAAATAAAAGCGGCa                                                                                                      <  1:330616/139‑1 (MQ=255)
                                  aaaTGAGTTGCCTGACCTTTTCGAAAAAAAGGGCGGGGTAAAAATTCGGCATGGATAATATACAGGGTATCTCAGACATAGGGGATGGACCCAATAACTCGATCCCAATTTAAACACCAACCTCAAAAAAAAACGCGGCa                                                                                                      <  1:932531/139‑1 (MQ=255)
                                       aTTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGta                                                                                                 <  2:855411/140‑1 (MQ=255)
                                       aTTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAAGAGACAGCGGATCTCAGAGATAGGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGta                                                                                                 <  2:395462/140‑1 (MQ=255)
                                          tGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTatat                                                                                              >  2:828810/1‑140 (MQ=255)
                                                aCCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTAc                                                                                        >  2:79009/1‑140 (MQ=255)
                                                aCCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTAc                                                                                        >  2:1388183/1‑140 (MQ=255)
                                                     ttCGATACACATGGCTGGGGAAAAATTATGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAAATCTCTATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGat                                                                                   >  2:1552598/1‑140 (MQ=255)
                                                     ttCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGat                                                                                   >  1:1276800/1‑140 (MQ=255)
                                                      tCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGatc                                                                                  >  2:1628477/1‑140 (MQ=255)
                                                      tCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGatc                                                                                  >  1:2080783/1‑140 (MQ=255)
                                                      tCGATAAACATTGATGGGGAAAAATTAGTTATGGAGAATATACATCGGATCTCAGAGATATGGGATTTACCCAAGATCTCGATCACAATTTAAACAGCATCCTCAAAAATAAAAGAGGAAAGCTATATACCTACCCGatc                                                                                  >  2:3214189/1‑140 (MQ=255)
                                                             acaTGGCTGGGGAAAAATTTTTTATTGAGAATATACAGCGGATCTCAGAGATATGGTATGGACCAAAGATCTCTATCCAAATTTAAAAAGCATCATAAAAAATAAAATATTCAATTTATATTCATACCCGATCAATCAgg                                                                           <  2:3486703/140‑1 (MQ=255)
                                                                   cTGGGGAAAAATTAGGTATGGATAATATACAGCGGATCTCAGAGATTTGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAg                                                                     <  2:374206/140‑1 (MQ=255)
                                                                    tGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGa                                                                    >  1:2541366/1‑140 (MQ=255)
                                                                    tGGGGAAAAATTAGGTATGGACAATATACAGCGGATCACAGAGATATGGGATGGACCAAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGa                                                                    <  1:3796248/140‑1 (MQ=255)
                                                                      gggAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACg                                                                  >  1:1044022/1‑140 (MQ=255)
                                                                      gggAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACg                                                                  >  2:688906/1‑140 (MQ=255)
                                                                      gggAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACg                                                                  >  1:1578050/1‑140 (MQ=255)
                                                                      gggAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACg                                                                  >  1:3486703/1‑140 (MQ=255)
                                                                         aaaaaTTAGTTATGGATAATATACAGCGGATCTAATATATATGGTATGGACCCAAGATCTCGATCCCAATTTAAACACCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGcc                                                               <  2:1968743/140‑1 (MQ=255)
                                                                         aaaaaTTAGGTATGTATAATATACAGCGTATCTCAGAGATATGGTATGGACCAAAGATCTCGATCCAAATTTAAACAGAATCCTAAAAAATAAACGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGcc                                                               <  1:2310584/140‑1 (MQ=255)
                                                                         aaaaaTTAGGTATGGATAATTTACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCAAATTTAAACAGCATCCACAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGcc                                                               <  1:3825940/140‑1 (MQ=255)
                                                                         aaaaaTTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAATATCTCGATCCCAATTTAAACAGCAACCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGcc                                                               <  2:3344267/140‑1 (MQ=255)
                                                                         aaaaaTTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAACGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGcc                                                               <  2:2282980/140‑1 (MQ=255)
                                                                         aaaaaTTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGcc                                                               <  2:3098654/140‑1 (MQ=255)
                                                                         aaaaaTTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCAAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGcc                                                               <  2:3381925/140‑1 (MQ=255)
                                                                            aaTTAGTTATGGAGAATATACAGCGGATCTCAGAGATATGGGATTGACCCAAGATCTCGATCCAAATTTAAACCGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTgg                                                            <  1:2619508/140‑1 (MQ=255)
                                                                             aTTAGGTATGGAGAATATACAGCGGATCTCAGAGATAAGGGATGGACCAAATATATAGATCCCAATTTAAACAGCATCCACAAAAATAAAATCGGAAAGGTATATGACAAACCGAACAATCAGGCAAAATACTGCCTGGc                                                           >  2:2857261/1‑140 (MQ=255)
                                                                                 ggTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCatat                                                       >  2:588785/1‑140 (MQ=255)
                                                                                      ggAGAATATCCAGCGTATCTCATAGATATGGGATGGACCCACGATCTCGATCCCAATTAAAACCGCAACCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCg                                                  <  1:2858115/140‑1 (MQ=255)
                                                                                      ggAGAATATACAGCGGATCTCAGAGCTATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCg                                                  <  2:2588261/140‑1 (MQ=255)
                                                                                        ataaTATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCACGCAAAAGACGGCCTGGCATATAACCGCa                                                <  2:2479991/138‑1 (MQ=255)
                                                                                        ataaTATACAGCGCAGCTCAGAGATATGGGATGCACCCAATTTCTCGATCCAAATTTAAACCGCATCCTCAAAAATAAACGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCa                                                <  2:219594/138‑1 (MQ=255)
                                                                                        agaATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCa                                                <  2:2800076/140‑1 (MQ=255)
                                                                                        agaATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCa                                                <  2:1830747/140‑1 (MQ=255)
                                                                                        agaATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACACCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCa                                                <  1:1885017/140‑1 (MQ=255)
                                                                                        agaATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTGAAACAGCATCCTCAAAACTAACAGCGGCAAGCTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCa                                                <  2:2231499/140‑1 (MQ=255)
                                                                                        agaATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTAAAACAGCATCCACAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCa                                                <  2:2800124/140‑1 (MQ=255)
                                                                                        agaATATACAGCGGATCTCAGAGATATGGGATGGACCCAACATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCa                                                <  2:1146656/140‑1 (MQ=255)
                                                                                             ataCAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATAtt                                           >  2:3254705/1‑140 (MQ=255)
                                                                                                 aGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTCCCCGATCAATCAGGCAAAAGACGGCCTGGCATAGACCCGCAATATTTTAg                                       >  2:1482432/1‑140 (MQ=255)
                                                                                                 aGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAg                                       >  1:2368285/1‑140 (MQ=255)
                                                                                                 aGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAg                                       >  1:1649594/1‑140 (MQ=255)
                                                                                                 aGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAg                                       >  1:595641/1‑140 (MQ=255)
                                                                                                  gCGGATCTCAGAGATATGGGATGGACCCCAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAACGACGGCCTGGCATATCACCGCAATATTTTAGa                                      >  2:1723665/1‑140 (MQ=255)
                                                                                                  gCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGc                                                                                                       >  1:2786258/1‑75 (MQ=255)
                                                                                                  gCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGa                                      >  1:3012171/1‑140 (MQ=255)
                                                                                                  gCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCCATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGa                                      >  2:413241/1‑140 (MQ=255)
                                                                                                  gCGGATCTCAGAGATAGGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAAAAACCGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGa                                      <  1:3498190/140‑1 (MQ=255)
                                                                                                   cGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGAc                                     >  1:3706472/1‑140 (MQ=255)
                                                                                                    ggATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCAGATAACCGCAATATTTTAGAcc                                    >  2:1680668/1‑140 (MQ=255)
                                                                                                      aTCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGt                                  >  2:2767039/1‑140 (MQ=255)
                                                                                                      aTCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGt                                  >  2:1245469/1‑140 (MQ=255)
                                                                                                      aTCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGt                                  >  2:3401015/1‑140 (MQ=255)
                                                                                                      aTCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGt                                  >  2:1312090/1‑140 (MQ=255)
                                                                                                        ctcAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAACGCGGCAAGGTATATGCCTACCCGATCAGTCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTa                                <  1:672606/140‑1 (MQ=255)
                                                                                                        ctcAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCCGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTa                                <  1:3680881/140‑1 (MQ=255)
                                                                                                            gagaTATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAACAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGc                            <  1:2120970/140‑1 (MQ=255)
                                                                                                                tatGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTa                        >  2:2454637/1‑140 (MQ=255)
                                                                                                                tatGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTa                        >  2:1003662/1‑140 (MQ=255)
                                                                                                                  tGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAAc                      >  2:3109801/1‑140 (MQ=255)
                                                                                                                  tGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAAc                      >  1:173872/1‑140 (MQ=255)
                                                                                                                  tGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAAc                      >  2:1107337/1‑140 (MQ=255)
                                                                                                                     gATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTCCCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACccg                   >  1:2431293/1‑140 (MQ=255)
                                                                                                                     gATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACccg                   >  1:3135503/1‑140 (MQ=255)
                                                                                                                     gATGGACCCAAGATCTCGATCCAAATTTAAAAAGCATCCTCAAAAATAAAAGAGGCAAGGTATATGCCTAACCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTAAGTAATTAAAccg                   >  1:460724/1‑140 (MQ=255)
                                                                                                                         gACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAATCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGGTACGGCATTAACCCGCCgg               >  1:1947636/1‑140 (MQ=255)
                                                                                                                         gACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTa                                >  1:1697493/1‑123 (MQ=255)
                                                                                                                         gACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACCCGCCgg               >  1:2269685/1‑140 (MQ=255)
                                                                                                                          aCCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACCCGCCGGa              >  2:2966390/1‑140 (MQ=255)
                                                                                                                          aCCCAAGATCTAGATCCCAATTTAAACAGAATCCTCAAAAATAAAAGCCGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACCCGCCGGa              <  2:2541366/140‑1 (MQ=255)
                                                                                                                              aaGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACCCGCCGGAAAcc          >  1:2487517/1‑140 (MQ=255)
                                                                                                                               aGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACCCGCCGGAAACCa         >  2:1755947/1‑140 (MQ=255)
                                                                                                                               aGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCAGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACCCGCCGGAAACCa         >  2:1342832/1‑140 (MQ=255)
                                                                                                                                    tcGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACCCGCCGGAAACCATGGAt    >  2:2140535/1‑140 (MQ=255)
                                                                                                                                    tcGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACCCGCCGGAAACCATGGAt    >  1:1515212/1‑140 (MQ=255)
                                                                                                                                    tcGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACCCGCCGGAAACCATGGAt    <  1:1808265/140‑1 (MQ=255)
                                                                                                                                    tcGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACCCGCCGGAAACCATGGAt    >  2:1455753/1‑140 (MQ=255)
                                                                                                                                    tcGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAAGATTTTAGACCGGTACGGCATTAACCCGCCGGAAACCATGGAt    >  1:3772002/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                      
ACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGGCCTGGCATATAACCGCAATATTTTAGACCGTTACGGCATTAACCCGCCGGAAACCATGGATGA  >  NZ_CP034943/2900654‑2900927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: