| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_CP034943 | 2,979,248 | T→C | 27.6% | noncoding (1553/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP034943 | 2,979,248 | 0 | T | C | 27.6% | ‑3.9 / 9.6 | 20 | noncoding (1553/2928 nt) | EO946_RS15430 | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (2/12); new base C (2/3); total (4/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01 | |||||||||||
TCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCA > NZ_CP034943/2979124‑2979383 | tcgcctcGCCTACCTGTGTCGGGTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTGCTTGGCAGTGTGGAATCAGGAACGTCGCTACTAGATTTCGCGCGCCATCACAGCGCGGCCGGATGGGCAAGGGATTTGc > 2:288032/4‑140 (MQ=1) tgggtCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCt < 2:1695444/137‑1 (MQ=1) actggCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCgg < 2:12076/137‑1 (MQ=1) tcgcACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACAGTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCaaa > 2:1835194/4‑139 (MQ=0) tccccTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATAc < 2:2800051/137‑1 (MQ=1) tccccTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATAc < 2:2455510/137‑1 (MQ=1) cgagcggCTTTTCTGGGCAGTGTGGACTCAGGAACGTCGCTCCTACATTTCGCTCGCCATCACCGCTGAGCCTTATGGGAAACGGATTGGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTa < 2:3389207/135‑1 (MQ=1) ttagCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTCTTTCCCAGCCTAACTCCTTGGACGCGGAAATCCAATACCGCGCGTACCCTCTCCTCCTGCGTCCCCCCATTGCTCAACTgg > 2:2529572/4‑140 (MQ=1) cgttactacATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTg < 1:2282202/113‑1 (MQ=1) cgttactacATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTg > 2:2282202/4‑116 (MQ=1) gcgactacCTGTCGCTCGCCATCACAGCTCAGCCTTATGGGCAACGGAGTTGCCTAGGTCCCAGCCTAACTGCTGGGACGCGGATATCCACTACCGCGCTTACCCTATCCTCCGGCGTCCCCCCATTGCTCAAATGGTga < 2:1431514/137‑1 (MQ=1) cgacctttCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTACCTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTgaggag < 2:1073128/135‑1 (MQ=0) tacctgtcgctcgcCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGaggagg < 1:2404675/134‑1 (MQ=1) tacctgtcgctcgcCATCACAGCTCAGCCGTATGGGAAACGGATTTGCCGAGTTCCCAGCCTAACTGCTTGGACGCGGAGATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCAGTGCTCAAATGGTGaggagg < 2:2217476/134‑1 (MQ=1) taaaTTTCGCTCGCCATCACAGCTCAGCCTTAAGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGaggagg < 1:1538374/137‑1 (MQ=1) atctcgcgcgcCATCACAGCTCAGCCGTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCGCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAggtgg < 1:3119351/137‑1 (MQ=0) gcacAGCCTTACGGGAAACGGAGTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCtg < 2:1617078/137‑1 (MQ=0) ccgtATGGGAAACGGATTTGCCTATTTCCCAGCCTTACTGCTTTGACGCGGATATCCAATACCGCGCTTACCCTATGCTCCGGCGTCCCCCCATTGCTCAAATGGTGATGATGTGGTACAGGAATATCAACCTGTTGTcc < 1:3120816/137‑1 (MQ=1) ctgaTGGGAAACGGATTTGCCTATGTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAACTGGTGAGGAGGTGGTACAGGAATATCAACCCGTTGTCCa < 2:3468276/137‑1 (MQ=0) ctgaTGGGAAACGGATTGGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCa < 1:2965360/137‑1 (MQ=1) | TCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCA > NZ_CP034943/2979124‑2979383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |