Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,052,465 G→A 6.1% T111T (ACG→ACA EO946_RS15780 → Na+/H+ antiporter subunit E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,052,4650GA 6.1% 747.3 / 24.6 231T111T (ACG→ACAEO946_RS15780Na+/H+ antiporter subunit E
Reads supporting (aligned to +/- strand):  ref base G (105/110);  new base A (7/7);  total (113/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 231 total aligned reads displayed.

GGGAACTGTACCTTGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGAAAAAGCCATACAGGAGGTG  >  NZ_CP034943/3052329‑3052600
                                                                                                                                        |                                                                                                                                       
gggAACTGTACCTTGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTa                                                                                                                                      <  2:3778302/140‑1 (MQ=255)
 ggAACTGTACCTTGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTaa                                                                                                                                     <  2:1066/140‑1 (MQ=255)
     cggTACCGTGCCAAGGTCAGTGTGCTTAAATCCGTCCGCTCGCCGAAACTGAAGATCAGACCGGCCATGTTCGCGTTTAAAACTGAGCTTACGACGGATTGGGACATCACCCTGCTGTCACTGCTCATTACGTTAACGcc                                                                                                                                 <  2:2983290/138‑1 (MQ=255)
     atGTACCTTGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGcc                                                                                                                                 <  2:3141427/139‑1 (MQ=255)
       ttACCTTGGCAATGTTAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCCGACCGGGGATTTTCGCTTTTATAACTGAGCTTACGACGTATTGTGAAATGACCCTGCTGTCACTGCTCATTACGTTAACGCCgg                                                                                                                               <  1:634551/139‑1 (MQ=255)
        tACCTTGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCggg                                                                                                                              <  2:2301164/140‑1 (MQ=255)
        tACCTTGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCggg                                                                                                                              <  2:1773179/140‑1 (MQ=255)
            ttGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAAcc                                                                                                                          <  2:355447/140‑1 (MQ=255)
             tGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAATCTGAGCTTACGACGGATTGGGACATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCt                                                                                                                         >  2:1035972/1‑140 (MQ=255)
             tGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCt                                                                                                                         <  1:2639886/140‑1 (MQ=255)
             tGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCt                                                                                                                         >  1:3693220/1‑140 (MQ=255)
             tGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCt                                                                                                                         <  1:843494/140‑1 (MQ=255)
             tGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCt                                                                                                                         <  1:1509415/140‑1 (MQ=255)
             tGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACAGCTCATTACTGTAACGCCGGGAccca                                                                                                                         >  2:3521421/1‑139 (MQ=255)
             tGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCCGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCt                                                                                                                         >  2:1784825/1‑140 (MQ=255)
             tGCCAATGTCAGTGTGATGAAATCAGTCTTATCTCCAAAACTGAATATCAGACCGGGTATTTTTGCTTTTAAAACTGAACTGAGGAAGGAGTGGGAAATCACCATGCTTTCTCTGCTCATTACATTAACGCCGGGAACCt                                                                                                                         >  1:115163/1‑140 (MQ=255)
             tGCCAATGTCAGTGTGATGAAATCAGTCTTATCTCCAAAACTGAATATCAGACCGGGTATTTTTGCTTTTAAAACTGAACTGACGAAGGACTGGGAAATCACCATGCTTTCTCTGCTCATTACATTAACGCCGGGAACCt                                                                                                                         >  1:1076215/1‑140 (MQ=255)
                  aTGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTg                                                                                                                    >  1:680695/1‑140 (MQ=255)
                  aTGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTg                                                                                                                    >  1:658093/1‑140 (MQ=255)
                  aTGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTg                                                                                                                    >  1:3595220/1‑140 (MQ=255)
                  aTGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTg                                                                                                                    >  1:1341571/1‑140 (MQ=255)
                  aTGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTg                                                                                                                    >  1:1516265/1‑140 (MQ=255)
                       aGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGa                                                                                                               <  2:432626/140‑1 (MQ=255)
                         tgtgCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGata                                                                                                             >  1:2544269/1‑140 (MQ=255)
                         tgtgCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGata                                                                                                             >  2:2498369/1‑140 (MQ=255)
                         tgtgCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGata                                                                                                             <  1:3493575/140‑1 (MQ=255)
                           tgCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATAtt                                                                                                           >  2:3047656/1‑140 (MQ=255)
                           tgCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATAtt                                                                                                           >  2:2257672/1‑140 (MQ=255)
                             cTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTc                                                                                                         >  2:3091008/1‑140 (MQ=255)
                             cTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTc                                                                                                         >  2:1242778/1‑140 (MQ=255)
                              ttAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTCCGACGGAGTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCa                                                                                                        <  2:2615160/140‑1 (MQ=255)
                                aaaTCGGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGa                                                                                                      >  1:1250345/1‑140 (MQ=255)
                                aaaTCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGa                                                                                                      >  1:3434116/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGcc                                                                                                                                 <  2:1618802/106‑1 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  2:625853/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  1:3071441/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  2:201672/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  2:3445028/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  2:1205725/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  2:3240779/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  1:1732742/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  1:2852043/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  1:3867654/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  2:810089/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  1:3350920/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  1:3351501/1‑140 (MQ=255)
                                       tCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTAAGACTGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAgg                                                                                               >  2:3497220/1‑140 (MQ=255)
                                        ccTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGa                                                                                              <  1:3280479/140‑1 (MQ=255)
                                          tATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACg                                                                                            <  1:1218446/140‑1 (MQ=255)
                                           aTCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGa                                                                                           <  1:3546454/140‑1 (MQ=255)
                                           aTCGCCGAAACTGAAGAACAGACCGGGCATTTTCGCTTTTACAACTGAGCTTACGCCGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGa                                                                                           <  2:3122220/140‑1 (MQ=255)
                                            tCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTTAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTa                                                                                                                                      <  1:1607729/96‑1 (MQ=255)
                                            tCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTa                                                                                                                                      <  1:3214132/96‑1 (MQ=255)
                                            tCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGAt                                                                                          >  2:3255947/1‑140 (MQ=255)
                                                 gAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCttt                                                                                     >  2:49399/1‑140 (MQ=255)
                                                 gAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCttt                                                                                     >  2:3474294/1‑140 (MQ=255)
                                                           atCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCAcgc                                                                           <  1:2279085/140‑1 (MQ=255)
                                                            tCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCAcgcg                                                                          <  1:2792033/140‑1 (MQ=255)
                                                            tCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCAcgcg                                                                          <  2:2134686/140‑1 (MQ=255)
                                                            tCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCAcgcg                                                                          <  1:23673/140‑1 (MQ=255)
                                                            tCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCAcgcg                                                                          <  2:3273152/140‑1 (MQ=255)
                                                            tCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCAcgcg                                                                          <  1:2003859/140‑1 (MQ=255)
                                                             cAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGa                                                                         <  2:1505574/140‑1 (MQ=255)
                                                             cAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGa                                                                         <  2:1290443/140‑1 (MQ=255)
                                                             cAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGa                                                                         <  2:741866/140‑1 (MQ=255)
                                                             cAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGa                                                                         <  1:3783347/140‑1 (MQ=255)
                                                             cAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGa                                                                         <  2:2598707/140‑1 (MQ=255)
                                                              aGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGTAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGAt                                                                        <  2:3694166/140‑1 (MQ=255)
                                                              aGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGAt                                                                        <  2:1428896/140‑1 (MQ=255)
                                                              aGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGAt                                                                        <  2:2893696/140‑1 (MQ=255)
                                                              aGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGAt                                                                        <  2:590/140‑1 (MQ=255)
                                                               gACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATg                                                                       <  2:2042537/140‑1 (MQ=255)
                                                               gACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATg                                                                       <  1:1691431/140‑1 (MQ=255)
                                                                  cGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGat                                                                    >  1:3741719/1‑140 (MQ=255)
                                                                    ggCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGatat                                                                  >  2:2418337/1‑140 (MQ=255)
                                                                    ggCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGatat                                                                  >  2:1139903/1‑140 (MQ=255)
                                                                            cGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATg                                                          <  1:2257672/140‑1 (MQ=255)
                                                                              cTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCg                                                        >  1:3482745/1‑140 (MQ=255)
                                                                                              tACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTg                                        <  2:892164/140‑1 (MQ=255)
                                                                                                     gATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCg                                 <  2:3346366/140‑1 (MQ=255)
                                                                                                     gATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCg                                 <  2:1341571/140‑1 (MQ=255)
                                                                                                     gATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCg                                 <  2:3595220/140‑1 (MQ=255)
                                                                                                        tGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGa                              <  2:2634936/140‑1 (MQ=255)
                                                                                                                 aCCCTGCTGTCACTGCTCATTACGTTACCGCCGGGAACCTTGGTGATGGATATTTCAGATGCCAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGa                     <  1:2940352/140‑1 (MQ=255)
                                                                                                                   ccTTCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTATTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGaaa                   <  1:1948679/140‑1 (MQ=255)
                                                                                                                   ccTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGaaa                   <  2:1538118/140‑1 (MQ=255)
                                                                                                                   ccTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGaaa                   <  1:3255947/140‑1 (MQ=255)
                                                                                                                    ctgctgTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGaaaa                  <  1:1139903/140‑1 (MQ=255)
                                                                                                                    ctgctgTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGaaaa                  <  2:1943210/140‑1 (MQ=255)
                                                                                                                    ctgctgTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGaaaa                  <  1:2418337/140‑1 (MQ=255)
                                                                                                                      gctgGCCCTGCTCATCACGTTACCGCCGGGCACCGTGGTGATGGATATGTCAGCTGACAGGACGATTCTTTAGATTCACGCGAGTGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCTTTTGAAAAAg                <  2:258322/140‑1 (MQ=255)
                                                                                                                       ctttctcTGCTCATTACATTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGAACAATACTTTATATTCATGCAATGGATATCGAGGATGCGGAAAAAGCGATTTTTGACATTCGGGAGTCATTTGAAAAAGc               <  2:2406703/134‑1 (MQ=255)
                                                                                                                          tCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGAAAAAGCCAt            >  1:732959/1‑140 (MQ=255)
                                                                                                                            tcTGCTCATTACATTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGAACAATACTTTATATTCATGCAATGGATATCGAGGATGCGGAAAAAGCGATTTTTGACATTCGGGAGTCATTTGAAAAAGCCATAc          <  1:3316986/139‑1 (MQ=255)
                                                                                                                            aCTGCTCATTACGTTAACGCCGGGACCCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGAAAAAGCCATAc          <  1:1205725/140‑1 (MQ=255)
                                                                                                                            aCTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGACAAAGCCATTTTTGATATTCGGGAGTCATTTGAAAAAGCCATAc          >  1:1435316/1‑140 (MQ=255)
                                                                                                                            aCTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGAAAAAGCCATAc          <  1:3240779/140‑1 (MQ=255)
                                                                                                                            aCTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGAAAAAGCCATAc          >  1:2084063/1‑140 (MQ=255)
                                                                                                                                 tCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGAAAAAGCCATACaggag     >  1:3193177/1‑140 (MQ=255)
                                                                                                                                    ttACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGAAAAAGCCATACAGGAGGTg  <  1:2126585/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
GGGAACTGTACCTTGCCAATGTCAGTGTGCTTAAATCAGTCCTATCGCCGAAACTGAATATCAGACCGGGCATTTTCGCTTTTAAAACTGAGCTTACGACGGATTGGGAAATCACCCTGCTGTCACTGCTCATTACGTTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGGACGATTCTTTATATTCACGCGATGGATATTGAGGATGCGGAAAAAGCCATTTTTGATATTCGGGAGTCATTTGAAAAAGCCATACAGGAGGTG  >  NZ_CP034943/3052329‑3052600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: