Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,169,618 G→A 5.3% I63I (ATC→ATT EO946_RS16390 ← carboxymuconolactone decarboxylase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,169,6180GA 5.3% 746.1 / 20.5 225I63I (ATC→ATTEO946_RS16390carboxymuconolactone decarboxylase family protein
Reads supporting (aligned to +/- strand):  ref base G (104/108);  new base A (6/6);  total (110/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

Only 100 of 225 total aligned reads displayed.

TGGCTAAGAGCCGCGCCGCCTCCAAATGCCGCTGCCACACTGACGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTG  >  NZ_CP034943/3169484‑3169752
                                                                                                                                      |                                                                                                                                      
tGGCTAAGAGCCGCGCCGCCTCCAAATGCCGCTGCCACACTGACGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTcc                                                                                                                                   >  1:277955/1‑140 (MQ=255)
      agagCCGCGCCGCCTCCAAATGCCGCTGCCACACTGACGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGc                                                                                                                             >  1:46540/1‑140 (MQ=255)
          ccgcgccgcCTCCAAATGCCGCTGCCACACTGACGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATc                                                                                                                         <  2:3255958/140‑1 (MQ=255)
             cgccgcCTCCAAATGCCGCTGCCACACTGACGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGc                                                                                                                      >  1:3238424/1‑140 (MQ=255)
                  ccTCCAAATGCCGCTGCCACACTGACGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATCTTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGttt                                                                                                                 >  1:865149/1‑140 (MQ=255)
                              gCTGCCACACTGACGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCAGTTTTTCTCTTtcagt                                                                                                     <  2:2292691/140‑1 (MQ=255)
                                tGCCACACTGACGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCGGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTtcagtca                                                                                                   <  2:3161015/140‑1 (MQ=255)
                                tGCCACACTGACGGCTTCGAGAATGATTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTtcagtca                                                                                                   <  2:2948766/140‑1 (MQ=255)
                                         gACGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGc                                                                                          <  2:3399609/140‑1 (MQ=255)
                                          aCGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGACTTGcc                                                                                         >  1:3384591/1‑140 (MQ=255)
                                          aCGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGcc                                                                                         >  2:57689/1‑140 (MQ=255)
                                           cGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCg                                                                                        <  2:794287/140‑1 (MQ=255)
                                           cGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCg                                                                                        <  2:2625804/140‑1 (MQ=255)
                                              cTTCGAGAATGTTTTCTTCTGTCGCTCCCTCCTCAAGACAGCCTTGTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCa                                                                                     <  1:3803261/140‑1 (MQ=255)
                                              cTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCa                                                                                     <  1:1008263/140‑1 (MQ=255)
                                              cTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCa                                                                                     <  1:2245004/140‑1 (MQ=255)
                                              cTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGATTTTCTCTTTCAGTCAGAGAATTGCCGGCa                                                                                     <  2:3149092/140‑1 (MQ=255)
                                              cTTCGAGAATGTTGTCTTCTGGCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCa                                                                                     <  1:2142738/140‑1 (MQ=255)
                                                   agaATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCa                                                                                <  2:1499379/140‑1 (MQ=255)
                                                   agaATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCa                                                                                <  2:1632637/140‑1 (MQ=255)
                                                   agaATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCa                                                                                <  2:441164/140‑1 (MQ=255)
                                                   agaATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCa                                                                                <  2:1000811/140‑1 (MQ=255)
                                                   agaATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCa                                                                                <  2:3360650/140‑1 (MQ=255)
                                                   agaATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCa                                                                                <  2:466255/140‑1 (MQ=255)
                                                   agaATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCa                                                                                <  2:580568/140‑1 (MQ=255)
                                                   agaATGTTGTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCa                                                                                <  2:2825110/140‑1 (MQ=255)
                                                                 tgacGCTCCCTGATCAAGACAGCCTTTTGTATGATAGATCATGCAATATTCGTCCTGAGCATTGATGGCAATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTCCCGGCAAAGCACTGTTCAGTaaatt                                                                  >  2:118320/4‑138 (MQ=255)
                                                                 tgacGCTCCCTGATCAAGACAGCCTTTTGTATGATAGATCATGCAATATTCGTCCTGAGCATTGATGGCAATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTCCCGGCAAAGCACTGTTCAGTaaatt                                                                  >  2:3294339/4‑138 (MQ=255)
                                                                        cccTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTaaa                                                           >  1:2538360/1‑140 (MQ=255)
                                                                        cccTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTaaa                                                           >  1:827749/1‑140 (MQ=255)
                                                                          cTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACt                                                         <  2:736220/140‑1 (MQ=255)
                                                                          cTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACt                                                         <  2:1870855/140‑1 (MQ=255)
                                                                          cTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACt                                                         <  2:247996/140‑1 (MQ=255)
                                                                          cTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACt                                                         <  1:57689/140‑1 (MQ=255)
                                                                                     aGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATc                                              >  2:3033231/1‑140 (MQ=255)
                                                                                       ccTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAg                                            >  2:1244148/1‑140 (MQ=255)
                                                                                       ccTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAg                                            >  2:3634250/1‑140 (MQ=255)
                                                                                       ccTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAg                                            >  1:1165452/1‑140 (MQ=255)
                                                                                        cTTTTGTGTGATAGAACATGCAATATTCATCCTGGGCATTGATTGCGATTCCGAGGGCGATACGCTGTTTTTCTCTTTCAGTCCGAGAATTGCCGGCAAAGCATTTTTCAGTAAACAAGTTAAAATTTCTTACCAtaacc                                           >  1:3673997/1‑136 (MQ=255)
                                                                                              tgtgATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAg                                                                                                                       >  2:1566487/1‑58 (MQ=255)
                                                                                              tgtgATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAg                                                                                                                       <  1:1566487/58‑1 (MQ=255)
                                                                                                 gATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCAttttt                                  >  2:2282816/1‑140 (MQ=255)
                                                                                                 gATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCAttttt                                  >  2:3064127/1‑140 (MQ=255)
                                                                                                 gATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCAttttt                                  >  2:2689450/1‑140 (MQ=255)
                                                                                                 gATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGAATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCAttttt                                  >  2:2771356/1‑140 (MQ=255)
                                                                                                        cATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCa                                                                                     <  1:1308407/82‑1 (MQ=255)
                                                                                                        cATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCaa                           >  1:743228/1‑140 (MQ=255)
                                                                                                          tGCAATATTCCTCCTGTGCATTGAGGGCGTTTCCGAGGGCTATCAGCTGTTTGTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTGAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                         <  1:2817644/140‑1 (MQ=255)
                                                                                                          tGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                         >  1:1104363/1‑140 (MQ=255)
                                                                                                          tGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                         <  2:2045147/140‑1 (MQ=255)
                                                                                                          tGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                         <  2:2538360/140‑1 (MQ=255)
                                                                                                          tGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                         >  1:2567652/1‑140 (MQ=255)
                                                                                                          tGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCACATCAGCGGCATTTTTTGTTCAAAt                         >  1:910901/1‑140 (MQ=255)
                                                                                                               tatTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGcc                    >  2:3679299/1‑140 (MQ=255)
                                                                                                               tatTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGcc                    >  2:2246218/1‑140 (MQ=255)
                                                                                                                atTCATCCTGGGCATTGATGGCGATTCCGAGTGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCTTGAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCt                   >  1:184736/1‑140 (MQ=255)
                                                                                                                atTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAATTGTGCCt                   >  1:1196056/1‑140 (MQ=255)
                                                                                                                atTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCt                   >  2:1607084/1‑140 (MQ=255)
                                                                                                                atTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCt                   <  2:2996581/140‑1 (MQ=255)
                                                                                                                atTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCt                   >  1:1220613/1‑140 (MQ=255)
                                                                                                                 ttCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTa                  >  2:1336117/1‑140 (MQ=255)
                                                                                                                 ttCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTa                  >  2:3259994/1‑140 (MQ=255)
                                                                                                                 ttCATCCTGGGCATTGATGGCGATTCCGAGGGCGAGCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTa                  >  2:914983/1‑140 (MQ=255)
                                                                                                                    aTCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTTTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCACCGGCAATATTTGTTCAAATGTGCCTAAAc               >  2:1363679/1‑140 (MQ=255)
                                                                                                                    aTCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAAc               <  1:3707555/140‑1 (MQ=255)
                                                                                                                      ccTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCa                                                                                <  1:708158/73‑1 (MQ=255)
                                                                                                                      ccTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCa                                                                                >  2:708654/1‑73 (MQ=255)
                                                                                                                      ccTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTaa                                                                     >  2:2791181/1‑84 (MQ=255)
                                                                                                                         gggCATTGTTGGCGATTCCGAGGGCGATCCGCTGTTTTTCTCTTTCTGTCAGAGACTGGCCGGCAAAGCCTTGTTCAGTCACCTCGTGATACTGTCTTCCCATCAGCGGCAGTTTTTGTTCATATGTGCCTAAACCTCAt          >  1:3034869/1‑140 (MQ=255)
                                                                                                                         gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGtt                              >  1:3345106/1‑120 (MQ=255)
                                                                                                                         gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGtt                              >  1:2898590/1‑120 (MQ=255)
                                                                                                                         gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGtt                              <  2:1437650/120‑1 (MQ=255)
                                                                                                                         gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGtt                              >  1:1437650/1‑120 (MQ=255)
                                                                                                                         gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAAt          >  1:2501290/1‑140 (MQ=255)
                                                                                                                         gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAAt          >  1:2160210/1‑140 (MQ=255)
                                                                                                                         gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAAt          >  2:2878116/1‑140 (MQ=255)
                                                                                                                         gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAAt          >  1:2460193/1‑140 (MQ=255)
                                                                                                                         gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTATTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCGTAAACCTAAt          >  2:2084410/1‑140 (MQ=255)
                                                                                                                          ggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAAtt         >  1:2448444/1‑140 (MQ=255)
                                                                                                                           gCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAAttt        >  2:3432334/1‑140 (MQ=255)
                                                                                                                              ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata     <  2:758667/140‑1 (MQ=255)
                                                                                                                              ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata     <  2:852428/140‑1 (MQ=255)
                                                                                                                              ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata     <  2:3673997/140‑1 (MQ=255)
                                                                                                                              ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata     <  2:1540715/140‑1 (MQ=255)
                                                                                                                              ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata     <  2:2160210/140‑1 (MQ=255)
                                                                                                                              ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata     <  1:3693780/140‑1 (MQ=255)
                                                                                                                              ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata     <  1:2878116/140‑1 (MQ=255)
                                                                                                                              ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata     <  1:2665899/140‑1 (MQ=255)
                                                                                                                              ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata     <  1:1582319/140‑1 (MQ=255)
                                                                                                                              ttGATGGCGATTCCGAGGGCGATCAGCTGTTTGTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAATCTTTCTTCCCCTCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata     <  2:950149/140‑1 (MQ=255)
                                                                                                                              ttGATGGCGAGTCCGAGGGCGATCAGATGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata     <  1:1246944/140‑1 (MQ=255)
                                                                                                                              ttGATGGCAATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTCCCGGCAAAGCACTGTTCAGTAAATTCGTTAAATTTTCTTCCCATCAGCGGCATCTTTTGTTCAAATGTGCCTAAACCATGTTtata     <  1:3294339/140‑1 (MQ=255)
                                                                                                                              ttGATGGCAATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTCCCGGCAAAGCACTGTTCAGTAAATTCGTTAAATTTTCTTCCCATCAGCGGCATCTTTTGTTCAAATGTGCCTAAACCATGTTtata     <  1:1616788/140‑1 (MQ=255)
                                                                                                                              ttGAGGGCGATTCCGAGGGCGATCAGCTGTTTGTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata     <  2:2201318/140‑1 (MQ=255)
                                                                                                                               tGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATAc    <  1:275894/140‑1 (MQ=255)
                                                                                                                                gATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                         <  2:1811026/118‑1 (MQ=255)
                                                                                                                                gATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                         <  2:1724706/118‑1 (MQ=255)
                                                                                                                                gATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                         >  1:1811026/1‑118 (MQ=255)
                                                                                                                                gATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACt   >  2:968990/1‑140 (MQ=255)
                                                                                                                                 aTGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTGAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCGTAAACCTACTTTATACtc  >  2:3337409/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TGGCTAAGAGCCGCGCCGCCTCCAAATGCCGCTGCCACACTGACGGCTTCGAGAATGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTG  >  NZ_CP034943/3169484‑3169752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: