Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,169,675 T→C 5.3% Q44Q (CAA→CAG EO946_RS16390 ← carboxymuconolactone decarboxylase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,169,6750TC 5.3% 749.4 / 21.0 226Q44Q (CAA→CAGEO946_RS16390carboxymuconolactone decarboxylase family protein
Reads supporting (aligned to +/- strand):  ref base T (108/103);  new base C (6/6);  total (114/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

Only 100 of 226 total aligned reads displayed.

TGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATCCCTCCATCT  >  NZ_CP034943/3169539‑3169808
                                                                                                                                        |                                                                                                                                     
tGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCACTCTTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGt                                                                                                                                    >  2:1645815/1‑140 (MQ=255)
 gTTTTCTTCTGTCGCTCCCTCAGCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGACGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGtt                                                                                                                                   <  1:1200065/140‑1 (MQ=255)
       ttctGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTaa                                                                                                                             <  1:2241903/140‑1 (MQ=255)
       ttctGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTaa                                                                                                                             <  1:2707445/140‑1 (MQ=255)
          tgacGCTCCCTGATCAAGACAGCCTTTTGTATGATAGATCATGCAATATTCGTCCTGAGCATTGATGGCAATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTCCCGGCAAAGCACTGTTCAGTaaatt                                                                                                                          >  2:3308511/4‑138 (MQ=255)
              gCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGtt                                                                                                                      <  2:2754649/140‑1 (MQ=255)
                   cTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACt                                                                                                                 <  2:736220/140‑1 (MQ=255)
                   cTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACt                                                                                                                 <  1:57738/140‑1 (MQ=255)
                   cTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACt                                                                                                                 <  2:247996/140‑1 (MQ=255)
                   cTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACt                                                                                                                 <  1:930388/140‑1 (MQ=255)
                          aGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTcc                                                                                                          >  1:2551954/1‑140 (MQ=255)
                              aGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCCTTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATc                                                                                                      >  1:154696/1‑140 (MQ=255)
                              aGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATc                                                                                                      >  2:1353215/1‑140 (MQ=255)
                              aGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATc                                                                                                      >  1:2446349/1‑140 (MQ=255)
                                ccTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAg                                                                                                    >  2:2292439/1‑140 (MQ=255)
                                ccTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAg                                                                                                    >  2:2971617/1‑140 (MQ=255)
                                ccTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAg                                                                                                    >  2:3634250/1‑140 (MQ=255)
                                ccTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAg                                                                                                    >  2:964640/1‑140 (MQ=255)
                                ccTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAg                                                                                                    >  1:2480495/1‑140 (MQ=255)
                                ccTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAg                                                                                                    >  1:1165452/1‑140 (MQ=255)
                                     tgtgtgATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATAAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCa                                                                                               >  2:2779843/1‑140 (MQ=255)
                                          gATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCAttttt                                                                                          >  2:2282816/1‑140 (MQ=255)
                                          gATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCAttttt                                                                                          >  2:90853/1‑140 (MQ=255)
                                              gATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGtt                                                                                      >  2:3293856/1‑140 (MQ=255)
                                                 cATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCaa                                                                                   >  1:743228/1‑140 (MQ=255)
                                                   tGCAATATTCCTCCTGTGCATTGAGGGCGTTTCCGAGGGCTATCAGCTGTTTGTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTGAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                                                                                 <  1:2817644/140‑1 (MQ=255)
                                                   tGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                                                                                 >  1:3236017/1‑140 (MQ=255)
                                                   tGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                                                                                 <  2:827749/140‑1 (MQ=255)
                                                   tGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                                                                                 >  1:2567652/1‑140 (MQ=255)
                                                   tGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                                                                                 >  1:1104363/1‑140 (MQ=255)
                                                        tatTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGcc                                                                            >  2:2246218/1‑140 (MQ=255)
                                                         atTCATCCTGGGCATTGATGGCGATTCCGAGTGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCTTGAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCt                                                                           >  1:184736/1‑140 (MQ=255)
                                                         atTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAATTGTGCCt                                                                           >  1:1196056/1‑140 (MQ=255)
                                                         atTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCt                                                                           >  2:1401992/1‑140 (MQ=255)
                                                         atTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCt                                                                           >  2:1607084/1‑140 (MQ=255)
                                                         atTCATCCTGGGCATTGAGGGCGATTCCGAGCGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGTCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCt                                                                           <  2:2214053/140‑1 (MQ=255)
                                                          ttCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCTTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTa                                                                          >  2:3258899/1‑140 (MQ=255)
                                                          ttCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTa                                                                          >  2:1206975/1‑140 (MQ=255)
                                                          ttCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTa                                                                          >  2:2809925/1‑140 (MQ=255)
                                                             aTCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAAc                                                                       <  2:1167029/140‑1 (MQ=255)
                                                             aTCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAAc                                                                       <  1:3785849/140‑1 (MQ=255)
                                                             aTCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAAc                                                                       <  1:1401992/140‑1 (MQ=255)
                                                             aTCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAAc                                                                       <  2:61714/140‑1 (MQ=255)
                                                                  gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGtt                                                                                      <  2:2898590/120‑1 (MQ=255)
                                                                  gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGtt                                                                                      <  2:1437650/120‑1 (MQ=255)
                                                                  gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAAt                                                                  >  2:2878116/1‑140 (MQ=255)
                                                                  gggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAAt                                                                  >  2:1568102/1‑140 (MQ=255)
                                                                   ggCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAAtt                                                                 >  2:2540674/1‑140 (MQ=255)
                                                                       ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAACCTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata                                                             <  2:3674428/140‑1 (MQ=255)
                                                                       ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTCATTtata                                                             <  1:444730/140‑1 (MQ=255)
                                                                       ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata                                                             <  1:1582319/140‑1 (MQ=255)
                                                                       ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata                                                             <  1:25154/140‑1 (MQ=255)
                                                                       ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata                                                             <  2:3027563/140‑1 (MQ=255)
                                                                       ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata                                                             <  2:758667/140‑1 (MQ=255)
                                                                       ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata                                                             <  1:2456922/140‑1 (MQ=255)
                                                                       ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata                                                             <  1:3693780/140‑1 (MQ=255)
                                                                       ttGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata                                                             <  2:1540715/140‑1 (MQ=255)
                                                                       ttGATGGCGAGTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata                                                             <  1:899953/140‑1 (MQ=255)
                                                                       ttGATGGCGAGTCCGAGGGCGATCAGATGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTtata                                                             <  1:1246944/140‑1 (MQ=255)
                                                                       ttGATGGCAATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTCCCGGCAAAGCACTGTTCAGTAAATTCGTTAAATTTTCTTCCCATCAGCGGCATCTTTTGTTCAAATGTGCCTAAACCATGTTtata                                                             <  1:3308511/140‑1 (MQ=255)
                                                                       ttGATGGCAATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTCCCGGCAAAGCACTGTTCAGTAAATTCGTTAAATTTTCTTCCCATCAGCGGCATCTTTTGTTCAAATGTGCCTAAACCATGTTtata                                                             <  1:2879565/140‑1 (MQ=255)
                                                                         gATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                                                                                 >  1:1811999/1‑118 (MQ=255)
                                                                         gATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAAt                                                                                 <  2:1811999/118‑1 (MQ=255)
                                                                          aTGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTGAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCGTAAACCTACTTTATACtc                                                          >  2:3337409/1‑139 (MQ=255)
                                                                                aTTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAg                                                    <  2:1997033/140‑1 (MQ=255)
                                                                                aTTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAg                                                    >  2:207965/1‑140 (MQ=255)
                                                                                 ttCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAgc                                                   <  1:2125690/140‑1 (MQ=255)
                                                                                  tCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGAATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAgct                                                  >  2:1774568/1‑139 (MQ=255)
                                                                                    cGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGcgcg                                                <  2:2448444/140‑1 (MQ=255)
                                                                                    cGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGcgcg                                                <  2:3236017/140‑1 (MQ=255)
                                                                                          cGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTACACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGc                                          >  1:2970563/1‑140 (MQ=255)
                                                                                           gATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCa                                         <  1:3030614/140‑1 (MQ=255)
                                                                                            aTCAGCTGTGTTTCTCTTTCAGTCAGAGAAATGCCGGCAAAGCATTGTTCAGTAATCTCGTTAAACTTTCTTACCATCAGCGGCATTTTTTGTTCAAATGTGCCTATACCTAATTTATACTGCTTTAGCGCGTCCTGCAt                                        >  2:938759/1‑140 (MQ=255)
                                                                                                   gTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCAtttt                                                                                           <  2:2096955/82‑1 (MQ=255)
                                                                                                   gTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCAtttt                                                                                           >  1:2096955/1‑82 (MQ=255)
                                                                                                   gTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCct                                 <  1:1708254/140‑1 (MQ=255)
                                                                                                   gTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCct                                 <  2:2166950/140‑1 (MQ=255)
                                                                                                      tttCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTg                              >  2:3138842/1‑140 (MQ=255)
                                                                                                         ctctTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCt                           >  1:39120/1‑140 (MQ=255)
                                                                                                         ctctTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCt                           <  1:841686/140‑1 (MQ=255)
                                                                                                         ctctTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTACCGCGTCCTGCATGCTTCCTCTGTCt                           <  2:1845381/140‑1 (MQ=255)
                                                                                                         ctctTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGACTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCt                           <  2:1252148/140‑1 (MQ=255)
                                                                                                              tcagtcagAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGAtt                      <  1:827589/140‑1 (MQ=255)
                                                                                                              tcagtcagAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGAtt                      >  1:1769604/1‑140 (MQ=255)
                                                                                                                      agaATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGaaa              <  1:2795760/140‑1 (MQ=255)
                                                                                                                      agaATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGaaa              >  1:3398891/1‑140 (MQ=255)
                                                                                                                           tGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATccc         >  2:3111872/1‑140 (MQ=255)
                                                                                                                           tGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATccc         >  2:2105040/1‑140 (MQ=255)
                                                                                                                           tGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATccc         >  2:3355711/1‑140 (MQ=255)
                                                                                                                           tGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATccc         >  2:1035931/1‑140 (MQ=255)
                                                                                                                           tGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATccc         >  2:3807300/1‑140 (MQ=255)
                                                                                                                           tGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATccc         >  2:395973/1‑140 (MQ=255)
                                                                                                                            gCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATAGTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCATAGAAAATccgg        >  1:2042285/1‑138 (MQ=255)
                                                                                                                              cGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATCCCTcc      <  2:1506328/140‑1 (MQ=255)
                                                                                                                               ggCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATCCCTCCa     <  2:1139523/140‑1 (MQ=255)
                                                                                                                                gCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATCCCTCCAt    <  1:3048101/140‑1 (MQ=255)
                                                                                                                                gCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATCCCTCCAt    <  1:3634250/140‑1 (MQ=255)
                                                                                                                                gCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATCCCTCCAt    >  1:3530361/1‑140 (MQ=255)
                                                                                                                                gCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTGAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATCCCTCCAt    <  2:2666017/140‑1 (MQ=255)
                                                                                                                                  aaaGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATCCCTCCATCt  <  1:2665915/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TGTTTTCTTCTGTCGCTCCCTCATCAAGACAGCCTTTTGTGTGATAGATCATGCAATATTCATCCTGGGCATTGATGGCGATTCCGAGGGCGATCAGCTGTTTTTCTCTTTCAGTCAGAGAATTGCCGGCAAAGCATTGTTCAGTAAACTCGTTAAACTTTCTTCCCATCAGCGGCATTTTTTGTTCAAATGTGCCTAAACCTAATTTATACTGCTTTAGCGCGTCCTGCATGCTTCCTCTGTCTTGATTCAAAGAAAATCCCTCCATCT  >  NZ_CP034943/3169539‑3169808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: