BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,285,491	T→C	5.5%	I304V (ATT→GTT)	EO946_RS16995 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,285,491	0	T	C	5.5%	1476.6 / 51.4	489	I304V (ATT→GTT)	EO946_RS16995	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base T (202/257); new base C (18/9); total (221/268)
Fisher's exact test for biased strand distribution p-value = 2.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 490 total aligned reads displayed.

ACGATGGCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAT  >  NZ_CP034943/3285352‑3285629

  gATGGCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAg                                                                                                                                          >  2:2726094/1‑140 (MQ=34)

  gATGGCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAg                                                                                                                                          <  1:3750870/140‑1 (MQ=34)

    tGGCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCt                                                                                                                                        >  1:2336223/1‑140 (MQ=34)

    tGGCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCt                                                                                                                                        >  2:2176046/1‑140 (MQ=34)

      gCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCttt                                                                                                                                      >  2:3292865/1‑140 (MQ=34)

       cGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTc                                                                                                                                     >  1:3415043/1‑140 (MQ=34)

          tCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGa                                                                                                                                  <  2:1687099/140‑1 (MQ=34)

          tCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGa                                                                                                                                  <  1:3001333/140‑1 (MQ=34)

           cGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGAt                                                                                                                                 >  1:2412507/1‑140 (MQ=34)

            gTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATa                                                                                                                                <  1:1272209/140‑1 (MQ=34)

            gTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATa                                                                                                                                <  1:2536740/140‑1 (MQ=34)

                 tCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGt                                                                                                                           <  2:1837808/140‑1 (MQ=34)

                 tCGATCAATGCTGTTAAGATAGAACGTGTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCACGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGt                                                                                                                           <  1:2270674/140‑1 (MQ=21)

                 tCGAGCAATGCTGTTAAGCTAGACCGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCAGATACTCGt                                                                                                                           <  1:978931/140‑1 (MQ=255)

                   gATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTaa                                                                                                                         <  1:2968460/140‑1 (MQ=34)

                   gATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTaa                                                                                                                         <  1:95855/140‑1 (MQ=34)

                    aTCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAg                                                                                                                        >  2:1307949/1‑140 (MQ=34)

                    aTCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAg                                                                                                                        >  2:2364243/1‑140 (MQ=34)

                     tCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGa                                                                                                                       <  2:1556479/140‑1 (MQ=34)

                         tGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGta                                                                                                                   >  1:288093/1‑140 (MQ=34)

                         tGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGta                                                                                                                   <  1:3342202/140‑1 (MQ=34)

                         tGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGGTAATAGCTTTCTGATACTCGTAAGATGta                                                                                                                   >  2:1602594/1‑140 (MQ=21)

                           cTGTTAAGATAGAACGTTTCAGACTTTCGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATgctgt                                                                                                                 <  1:3815770/140‑5 (MQ=19)

                             gTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATaca                                                                                                               <  2:638091/140‑1 (MQ=34)

                             gTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCCGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATaca                                                                                                               <  1:2457944/140‑1 (MQ=21)

                              ttAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTaa                                                                                                                         >  1:3104621/1‑129 (MQ=35)

                              ttAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATacac                                                                                                              >  2:957826/1‑140 (MQ=34)

                                   aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAc                                                                                                         <  2:2245608/140‑1 (MQ=34)

                                   aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAc                                                                                                         >  2:1995707/1‑140 (MQ=34)

                                   aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAc                                                                                                         <  2:398103/140‑1 (MQ=34)

                                   aTAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTGTCTGATACTCGTAAGATGTATACACAGAAc                                                                                                         <  1:1219704/140‑1 (MQ=21)

                                    tAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAAcc                                                                                                        <  1:486770/140‑1 (MQ=34)

                                      gAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCtt                                                                                                      >  2:1633554/1‑140 (MQ=34)

                                      gAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCtt                                                                                                      >  2:88725/1‑140 (MQ=34)

                                      gAACGTTTCAGACTTTAGGGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTCTCTGATACTCGTAAGATGTATACACAGAACCtt                                                                                                      <  1:1032867/140‑1 (MQ=21)

                                       aaCGTTTCAGAATTTAGTGAGGAATGAAACGATTCCATGGATGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATTGTAATGCCTTTTGTTTTAATAGCTTTCTGATACTCGTAAGATGTATAAACAGAACCTTg                                                                                                     >  2:1734626/1‑140 (MQ=11)

                                        aCGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTgg                                                                                                    >  2:2626673/1‑140 (MQ=34)

                                        aCGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTgg                                                                                                    >  2:1016114/1‑140 (MQ=34)

                                         cGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  1:2117846/140‑1 (MQ=34)

                                         cGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  1:1510772/140‑1 (MQ=34)

                                           tttCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCa                                                                                                 <  2:535468/140‑1 (MQ=34)

                                             tCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACt                                                                                               >  1:1795608/1‑140 (MQ=34)

                                                      aGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAacac                                                                                      >  1:2232282/1‑140 (MQ=34)

                                                      aGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAacac                                                                                      >  1:2924563/1‑140 (MQ=34)

                                                       gTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACg                                                                                     <  2:1943436/140‑1 (MQ=34)

                                                       gTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACg                                                                                     <  1:1711609/140‑1 (MQ=34)

                                                       gTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACg                                                                                     <  1:2814249/140‑1 (MQ=34)

                                                             aaTGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTtc                                                                               >  2:2264334/1‑140 (MQ=34)

                                                             aaTGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTtc                                                                               >  2:1373187/1‑140 (MQ=34)

                                                                 aaaCGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAg                                                                           <  1:267457/140‑1 (MQ=34)

                                                                 aaaCGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAg                                                                           >  2:2937282/1‑140 (MQ=34)

                                                                 aaaCGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAg                                                                           <  2:1852649/140‑1 (MQ=34)

                                                                      aTTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAgt                                                                      >  2:2405389/1‑140 (MQ=34)

                                                                       ttCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGt                                                                                                   <  2:1269524/110‑1 (MQ=35)

                                                                       ttCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGc                                                                         <  1:3612455/136‑1 (MQ=34)

                                                                               aGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGc                                                             <  1:1654464/140‑1 (MQ=32)

                                                                                     tATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATAcaca                                                                                                             >  2:2514930/1‑86 (MQ=33)

                                                                                        cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  1:39777/140‑1 (MQ=32)

                                                                                        cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                    <  1:1358346/140‑1 (MQ=32)

                                                                                         aGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTa                                                   >  1:581094/1‑140 (MQ=33)

                                                                                           cGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATg                                                 >  2:3212525/1‑140 (MQ=34)

                                                                                           cGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATg                                                 >  1:2055463/1‑140 (MQ=34)

                                                                                            gggCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGt                                                <  2:3380123/140‑1 (MQ=34)

                                                                                            gggCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGt                                                <  2:1527508/140‑1 (MQ=34)

                                                                                              gCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCt                                              <  1:1914505/140‑1 (MQ=34)

                                                                                              gCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCt                                              <  2:1076121/140‑1 (MQ=34)

                                                                                                 tCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACACGTAAGATGTCTACACAGACCCTTGGTCACTATGTAACACGCAGTTCTCAGGCCGTGTTCGCAGCTGATTAAGTCTGTCTAAg                                           >  2:2282307/1‑140 (MQ=11)

                                                                                                   ccTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGAc                                         <  2:3091381/140‑1 (MQ=34)

                                                                                                          gggACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCt                                  <  1:1131293/140‑1 (MQ=34)

                                                                                                          gggACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCt                                  <  2:361402/140‑1 (MQ=34)

                                                                                                            gACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGTCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCtgt                                >  1:1953815/1‑140 (MQ=21)

                                                                                                            gACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCtgt                                >  1:792961/1‑140 (MQ=34)

                                                                                                               aTGCTCATTGTAATGCCTTTTGTGTTACTATCTTTCTGATACTCTTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTc                             <  2:3413930/140‑1 (MQ=255)

                                                                                                               aTGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTc                             <  2:3744195/140‑1 (MQ=34)

                                                                                                               aTGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTc                             <  1:1572394/140‑1 (MQ=34)

                                                                                                               aTGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTc                             <  2:1419788/140‑1 (MQ=34)

                                                                                                                      tGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAATGTCTGTGTCCTGCTTa                      >  2:278496/1‑140 (MQ=21)

                                                                                                                      tGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTa                      >  2:2420854/1‑140 (MQ=34)

                                                                                                                      tGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTa                      >  2:3678268/1‑140 (MQ=34)

                                                                                                                      tGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACAAAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTa                      >  2:3018590/1‑140 (MQ=21)

                                                                                                                           aTGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGGAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATcccc                 <  1:9176/140‑1 (MQ=255)

                                                                                                                           aTGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATcccc                 >  1:2587838/1‑140 (MQ=39)

                                                                                                                           aTGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATcccc                 >  1:306433/1‑140 (MQ=39)

                                                                                                                           aTGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATcccc                 >  1:3292456/1‑140 (MQ=39)

                                                                                                                           aTGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATcccc                 <  1:3517295/140‑1 (MQ=39)

                                                                                                                            tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                >  1:1397826/1‑140 (MQ=40)

                                                                                                                            tGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCa                >  1:844856/1‑140 (MQ=40)

                                                                                                                                 tttGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt           >  2:2840214/1‑140 (MQ=255)

                                                                                                                                 tttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt           >  2:818175/1‑140 (MQ=255)

                                                                                                                                 tttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt           >  2:3821424/1‑140 (MQ=255)

                                                                                                                                  ttGTGTTACCAGCTTTCTGCGACTCGTAAGATGGATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTACGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTa          <  1:1873913/140‑1 (MQ=11)

                                                                                                                                  ttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTa          <  2:719489/140‑1 (MQ=255)

                                                                                                                                  ttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTa          <  1:2730659/140‑1 (MQ=255)

                                                                                                                                   tgtgTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTaa         >  2:349856/1‑140 (MQ=255)

                                                                                                                                         aaTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCACCAATCGTAAAAGCaa   >  2:3555793/1‑140 (MQ=255)

                                                                                                                                          aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt  <  1:1058594/140‑1 (MQ=255)

                                                                                                                                          aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt  <  1:705517/140‑1 (MQ=255)

                                                                                                                                          aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt  <  1:635695/140‑1 (MQ=255)

                                                                                                                                          aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt  >  1:3660255/1‑140 (MQ=255)

                                                                                                                                          aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt  <  1:1004805/140‑1 (MQ=255)

ACGATGGCGGTCGTGGTTCGATCAATGCTGTTAAGATAGAACGTTTCAGACTTTAGTGAGGAATGAAACGATTCCATGGAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAT  >  NZ_CP034943/3285352‑3285629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`