Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 177,101 G→T 9.1% M1F (TTG→TTT) † EO946_RS01025 → SulP family inorganic anion transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943177,1010GT 9.1% 511.9 / 8.9 201M1F (TTG→TTT) †EO946_RS01025SulP family inorganic anion transporter
Reads supporting (aligned to +/- strand):  ref base G (58/118);  new base T (23/2);  total (81/120)
Fisher's exact test for biased strand distribution p-value = 1.37e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

CCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATGGTCGGT  >  NZ_CP034943/176962‑177239
                                                                                                                                            |                                                                                                                                          
ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTGTTCTCCGGTCGTGTTCCGTTAATTTCTCCCGTGAAATAAATAAAGCTCCCAATACAACACAAAAGGTGCGGCTTTATTTTTTAAAAAGGAGTGGTTCTATATg                                                                                                                                            >  2:2964277/1‑140 (MQ=255)
   tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaat                                                                                                                                         <  2:1348782/140‑1 (MQ=255)
   tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGGTTTGAATTATCCCCTAAAAAAAAAAAATCGCCCAAAAAAAAAAAAAACGGGGGGGTTTATTTTTTTAAAAAGAGTCGttgtatttgtat                                                                                                                                         >  1:292643/1‑140 (MQ=255)
     ccTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaataa                                                                                                                                       <  1:1372046/140‑1 (MQ=255)
     ccTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaataa                                                                                                                                       <  1:258691/140‑1 (MQ=255)
     ccTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaataa                                                                                                                                       <  2:186765/140‑1 (MQ=255)
     ccTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaataa                                                                                                                                       <  1:39915/140‑1 (MQ=255)
         aaaaCAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCGTTTATTTGTACCGGAAAATAAACAAAACGGCCCATACAACAAATACCGGGGGGCGTTATTTTTTTTAAATGTGTGGGTTTATTTGTATAATAGt                                                                                                                                   >  1:2201159/1‑140 (MQ=255)
         aaaaCAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCCTAAAATAAAAAAAGCTCCCAATACAACAAATACGGTGCGGCTTTATTTTTTAAAAAGGAGTGGTTATATTTGgataaaatt                                                                                                                                   >  2:258691/1‑136 (MQ=255)
         aaaaCAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCAATTGTGCCGTAAAATAAAGAAAGCTCCCAAAACAAAAAATACCGTGCGGATTTATTTTTTAAAAAGGAATAGTTATATTTTAATAATAGt                                                                                                                                   >  2:391732/1‑140 (MQ=255)
           aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                                                                 <  1:1565452/140‑1 (MQ=255)
                caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTGATTTATCCCGTAAAATAAAGAAAGCTCCCAATACAAAAAATACGGGGCGGATTTATTTTTTAAAAAGGAGTGGTTATATTTTAATAATAGTTTTACAt                                                                                                                            >  2:1713349/1‑140 (MQ=255)
                caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTAAATTCTCCCGTTAAATAAACAAAGCTCCCAATACAACAAATACGGGGCGGATTTATTTTTTGAAATGGAGTCGTTCTGTTTGAAAAATAGTTTTACAt                                                                                                                            >  2:3516706/1‑140 (MQ=255)
                   aCTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTaa                                                                                                                         <  1:1756447/140‑1 (MQ=255)
                       aaCGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTTGTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTAGTTATATTTGAATAATATGTTTATATTAAagaa                                                                                                                     >  2:3606098/1‑138 (MQ=255)
                       aaCGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTAATTTATACCGTAAAATAAAGAAAACACACAAAACAAAAAAAAAGGTGGGGTTTTATTTTTTAAAAATGAGTCGTTATATTTTAATTAAAGTTttaaattaaataa                                                                                                                     >  2:3568724/1‑137 (MQ=255)
                       aaCGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATACCGTAAAATAAAGAAAGCTCCCAAAACAAAAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCCTTATATTTTAAAAATAGTTTTACATTAAagca                                                                                                                     >  2:1669217/1‑140 (MQ=255)
                       aaCGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTGATTTATCCCGTAAAATAAATAAAGCTCCCAATAAAACAAATACGGGGGGGATTTATTTTTTAAAAAGGAGTCGTTATATTTTTATAATATTTTTTCATTAAagaa                                                                                                                     >  2:1456633/1‑138 (MQ=255)
                        acttgtCCTCTTTTTCTCCGGTCGTTTTAATTTAAATTGTCCCGTAAAAAAAAAAAAACACACAAAACAAAAAAAAAAGGGGGGTTTTTTTTTTTAAAAATGTGTAGGTAGTGTTTTAATATAGTTTTTAATTaaataat                                                                                                                    >  1:2652428/4‑136 (MQ=255)
                           tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACACATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATCATAGTTTTACATTAAAGCAGCaa                                                                                                                 <  2:2541810/140‑1 (MQ=255)
                           tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                                                                 <  2:1069364/140‑1 (MQ=255)
                           tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAAGACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                                                                 <  2:122462/140‑1 (MQ=255)
                           tgtCCTCTTTTTCTCCGGTCGTTGTCCATTCATTTCTCCCGTAAACTAAAGAAAGCTCCCACTACCACAAAGACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTACAGCAGCaa                                                                                                                 <  1:921374/140‑1 (MQ=255)
                           tgtCCTCTGTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                                                                 <  2:2553401/140‑1 (MQ=255)
                             tCCTCTTTTTCTCCGGTCGTTTTCCTTTGATTTGTCCCCTAAAATAAAGAAATCACCCAAAACAAAAAAAAACGTGGGGGTTTATTTTTTAAAAATGAGTCGTTATATTTTAATAATAGTTTTAAATTAAATCAGCaaag                                                                                                               >  2:182241/1‑138 (MQ=255)
                                      tctcCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAACTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGaa                                                                                                      <  2:3365974/140‑1 (MQ=255)
                                      tctcCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGaa                                                                                                      <  2:898903/140‑1 (MQ=255)
                                                ttttCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGaaaa                                                                                            <  1:1614982/140‑1 (MQ=255)
                                                    ccTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACa                                                                                        <  2:292643/140‑1 (MQ=255)
                                                            ttCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCt                                                                                <  2:3458323/140‑1 (MQ=255)
                                                            ttCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCt                                                                                <  2:1097327/140‑1 (MQ=255)
                                                            gtcgcccGTAAAAGAAAGACAGCTCCCAATACAAGACCTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCt                                                                                <  2:2194776/136‑1 (MQ=255)
                                                                      aaaTAAAGAAAGCTCCCAATACAACACATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                                                                      <  1:182241/140‑1 (MQ=255)
                                                                      aaaTAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                                                                      <  1:127465/140‑1 (MQ=255)
                                                                      aaaTAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGATTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                                                                      <  2:596391/140‑1 (MQ=255)
                                                                         tAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTGATATTTGTATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATACGAAAAGACATTCTTGCTGGCATTTTAGGTg                                                                   >  1:541865/1‑140 (MQ=255)
                                                                           aagaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCg                                                                 >  2:475084/1‑140 (MQ=255)
                                                                             gaaagCTCCCAATACAACAAATACGGTGCTGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                                                               <  1:838527/140‑1 (MQ=255)
                                                                             gaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                                                               <  2:2379194/140‑1 (MQ=255)
                                                                             gaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                                                               <  2:623927/140‑1 (MQ=255)
                                                                             gaaagCTCACAATACACAAAATACGTTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATATTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                                                               <  2:804972/140‑1 (MQ=255)
                                                                               aatcTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAATGATTCGTTATATTTTAATAATAGTTTTACATTAAAGCAGCAATGGTTCTGGAATATCCGAAAAGACATTCTTGCTGGCATTTTTGTTGCGTTAg                                                             <  2:2262779/137‑1 (MQ=255)
                                                                                    cccAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTa                                                        <  1:1851879/140‑1 (MQ=255)
                                                                                    cccAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTa                                                        <  1:2765771/140‑1 (MQ=255)
                                                                                    cccAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTa                                                        <  1:3337276/140‑1 (MQ=255)
                                                                                     ccAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGACTATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGt                                                                >  1:2557680/1‑131 (MQ=255)
                                                                                             acaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGaa                                               >  2:2346330/1‑140 (MQ=255)
                                                                                             acaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGaa                                               >  2:2746301/1‑140 (MQ=255)
                                                                                                 aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTTTTTGCGTTAGCATTATTAACTGAAGCaa                                           >  2:2245942/1‑140 (MQ=255)
                                                                                                 aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCaa                                           >  1:305692/1‑140 (MQ=255)
                                                                                                 aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCaa                                           >  2:3302068/1‑140 (MQ=255)
                                                                                                   aCGGTGCGGATTTATTTTTTAAAAAGTAGTCGTTATATTTTTATAATAGTTTTTCATTAAATCATCAAAGGTTCCGTAATATCCGAAAAGAAATTATTTCTGGCATTTTATTTGCTTTAGCAATAAAAACAGAATCaaat                                         >  2:3782260/1‑138 (MQ=255)
                                                                                                   aCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTCATACCTGAAGCAAtt                                         >  1:779217/1‑140 (MQ=255)
                                                                                                      gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGGATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGc                                      >  2:1398376/1‑140 (MQ=255)
                                                                                                      gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGGATTAATAACTGAAGCAATTTGc                                      >  2:3252214/1‑140 (MQ=255)
                                                                                                      gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGc                                      >  1:142837/1‑140 (MQ=255)
                                                                                                      gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGc                                      >  2:1292739/1‑140 (MQ=255)
                                                                                                      gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGc                                      >  1:68407/1‑140 (MQ=255)
                                                                                                      gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGc                                      >  1:3870061/1‑140 (MQ=255)
                                                                                                      gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGc                                      >  1:2604385/1‑140 (MQ=255)
                                                                                                      gTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATACGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATAACTGAAGCAATTGGc                                      >  2:2878280/1‑140 (MQ=255)
                                                                                                        gCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtt                                    <  1:3184615/140‑1 (MQ=255)
                                                                                                        gCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGAAATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTTAAGCAATTGGCtt                                    >  2:3721759/1‑140 (MQ=255)
                                                                                                         cGGATTTATTTTTTAAAAAGGAGTCGTTATATTTTAATAATAGTTTTACATTAAATCAGCAATTGTTAGGGAATATCCGAAAATACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCttt                                   >  1:750636/1‑140 (MQ=255)
                                                                                                         cGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCttt                                   >  1:2998528/1‑140 (MQ=255)
                                                                                                         cGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTATTGCTGGCATTTTAGTTGCGTTAGCATTAATAACTGAATCAATTGGCttt                                   >  1:2115161/1‑140 (MQ=255)
                                                                                                         cGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATACGAAAAGACATTCTTGCTGGCATTTTTGTTGCGGTAGCATTAAAACCTGAAGCAATTGGCttt                                   >  1:2467382/1‑140 (MQ=255)
                                                                                                         cGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTAGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCttt                                   >  1:3637834/1‑140 (MQ=255)
                                                                                                          ttatttatttTTTAAAAAGGAGTCGTTATATTTTAATAATAGATTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                  <  1:3139728/138‑1 (MQ=255)
                                                                                                          gtatttatttTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                  <  1:1032355/138‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                  <  1:3311845/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                  <  1:3402228/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                  <  2:655311/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                  <  1:1745267/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                  <  1:285690/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                  <  2:3556357/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                  <  1:2298556/140‑1 (MQ=255)
                                                                                                          ggATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTAGGGAATTTCCGAAAAGACATTCTTGCTGGGATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCtttt                                  <  2:3481463/140‑1 (MQ=255)
                                                                                                               tatttTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCtatt                             <  2:305692/140‑1 (MQ=255)
                                                                                                               tatttTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCtatt                             <  2:41564/140‑1 (MQ=255)
                                                                                                                    tttAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGc                        <  2:2767808/140‑1 (MQ=255)
                                                                                                                    tttAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGc                        <  2:2719586/140‑1 (MQ=255)
                                                                                                                    tttAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGc                        <  1:3606098/140‑1 (MQ=255)
                                                                                                                    tttAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGc                        <  1:3766289/140‑1 (MQ=255)
                                                                                                                    tttAAAAAGGAGTCGTTATATTTGAATAATAGTTTGACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGc                        <  1:1107229/140‑1 (MQ=255)
                                                                                                                    tttAAAAAGGAGTCGTTATATTTGAATAATAGTTGTACATTAAAGCAGCCATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATCCCTGAAGCAATTGGCTTTTCTATTATAGc                        <  1:1951390/140‑1 (MQ=255)
                                                                                                                      tAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACAGTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTGTATTATAGGCg                      >  1:2190779/1‑140 (MQ=255)
                                                                                                                      tAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACAATAAAGCAGCAATGGTTCGGGAATATCCCAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATAACTGAAGCAAATGGCTTTTCTATTATATCCg                      >  1:43178/1‑140 (MQ=255)
                                                                                                                          aaGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGgtgt                  <  2:316713/140‑1 (MQ=255)
                                                                                                                                       tatTTGCATAATAGTTTTCCATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt      <  2:2610642/140‑1 (MQ=255)
                                                                                                                                       tatTTGAATAATAGTTTTACATTAAAGCAGCAATTGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt      <  2:2906824/140‑1 (MQ=255)
                                                                                                                                       tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACCTTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt      <  2:673988/140‑1 (MQ=255)
                                                                                                                                       tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt      <  2:3622125/140‑1 (MQ=255)
                                                                                                                                       tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt      <  2:396145/140‑1 (MQ=255)
                                                                                                                                       tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt      <  2:502183/140‑1 (MQ=255)
                                                                                                                                       tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt      <  2:1549120/140‑1 (MQ=255)
                                                                                                                                       tatTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAAGATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATGAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt      <  2:2509015/140‑1 (MQ=255)
                                                                                                                                       taattGAATAATAGTTTTACAATACAGCAGCAATGGTGCGGGAAGATCCGAAAAGACATTCTGGCTGGCATTTTAGTTGCGGTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggt      <  1:1220394/137‑1 (MQ=255)
                                                                                                                                        atTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggtc     <  1:475084/140‑1 (MQ=255)
                                                                                                                                         tttGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATggtcg    <  2:3013042/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
CCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATTGGCTTTTCTATTATAGCCGGTGTTGATCCGATGGTCGGT  >  NZ_CP034943/176962‑177239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: