Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,995 C→A 6.3% V197V (GTG→GTT degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9950CA 6.3% 848.0 / 3.1 319V197V (GTG→GTTdegUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base C (70/203);  new base A (37/2);  total (114/205)
Fisher's exact test for biased strand distribution p-value = 2.06e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 319 total aligned reads displayed.

AAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGA  >  NZ_CP034943/3446858‑3447133
                                                                                                                                         |                                                                                                                                          
          cccAGTAAAAGGCACGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACCGGTTTCTCa                                                                                                                                >  2:479608/1‑140 (MQ=255)
          cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTAACGGTTTTCTCa                                                                                                                                >  2:3104060/1‑140 (MQ=255)
          cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                                <  2:3871238/140‑1 (MQ=255)
          cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                                <  2:364864/140‑1 (MQ=255)
          cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                                <  1:1354127/140‑1 (MQ=255)
                  aaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATaaa                                                                                                                        <  1:147903/140‑1 (MQ=255)
                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTGACGGTTTTATCACTGATAAACa                                                                                                                      >  2:2805971/1‑140 (MQ=255)
                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATCATTTTTTGCAAAAAATTGCTCACAAGGTTTTTCACGGGTTTCTCACTGATAAACa                                                                                                                      >  1:3853497/1‑140 (MQ=255)
                     gCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACCACGGCTTGCTTACGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACaa                                                                                                                     <  2:1400152/140‑1 (MQ=255)
                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                                  <  1:756729/140‑1 (MQ=255)
                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                                  <  2:1213315/140‑1 (MQ=255)
                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGTTCGTGTCCATTCATTTTTTGCCAAATATTGCGAACATTTTTTTTGACGGTTTTGTCACTGTTaaaaatagat                                                                                                                 >  1:2600185/1‑133 (MQ=255)
                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCAAATGGTTTTTAAAGGTTTTATAACTGATAAACAAAGAt                                                                                                                 >  1:3589094/1‑140 (MQ=255)
                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAAATTCTCACAAGGTTTTTCACGGTTTTCTCACTGATAAACAATGAt                                                                                                                 >  2:110335/1‑140 (MQ=255)
                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTTCTAAAATGGTTTTTAAAGGTTTTATAAATTATAAAAAAtttt                                                                                                                 >  2:3236622/1‑137 (MQ=255)
                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACCGTTTTATCACTTATAAAAAATGAt                                                                                                                 >  2:2775472/1‑140 (MQ=255)
                          tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                                <  1:546830/140‑1 (MQ=255)
                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTTCTCACATTGTTTTTCAAGGTTTTCTCACAGATAAACAATTATTCACCAATaac                                                                                                      >  2:2122889/1‑138 (MQ=255)
                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATAATGCTCACAAGGTTTTTAAAGGTTTTCTCACTGATAAACAAAGATTAACCAAAAcc                                                                                                      >  2:2643501/1‑140 (MQ=255)
                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAAAGGCTTGCGTCCTGTAGTTTACAATAATTTTTTGCAAAATATAGATCACAAGGTTTTTAATTGTTTTATCAATGATAAAAAAAGATTAACCAATAcc                                                                                                      >  2:1739723/1‑140 (MQ=255)
                                    taCTATCTCAGTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTCAAAATAATTCTAACATGGTTTTTCACGGTTTTATCACTGATAAACAATGATTCAACAATaaa                                                                                                      >  2:2334073/1‑138 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCAATCATTTTTTTAAAAAAAATTGTCAAATGGTTTTTCACGGTTTTATAACTGATAAAAAATGATTAAACAATaaccccg                                                                                                  >  1:2734200/1‑134 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAATTTCTCACATTGTTTTTAACGGTTTTATAAAAGATAAAAAAAGATTCACCAAAAACGCgg                                                                                                  >  2:1904979/1‑140 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAATTTCTCACATGGTTTTTCAAGGTTTTCTAACTGATAAAAAAAGATTAACCAAAAACGCgg                                                                                                  >  1:2800832/1‑140 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTGCTCACATGGTTTTTAAAGGTTTTATAACAGATAAACAAAGATTCACCAATACcgccg                                                                                                  >  2:3818101/1‑138 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTTCAAAATATTTCTCACAAGGTTTTTGACGGTTTTCTAACTGATAAAAAAAGATTGAACAAAAACGCgg                                                                                                  >  1:3067798/1‑140 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAAATAATTTTTTTCAAAAAATTGCTCACATTGTTTTTAACCGGTTTATCAAATCTAAAAAAAGGTTTACAAATAACGCgg                                                                                                  >  1:3208566/1‑140 (MQ=255)
                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTAAAAATAATGCTCACATGGTTTTTAAAGGTTTTATAAATGATAAACAATTATTAACAAATACACCGGt                                                                                                 >  2:1990869/1‑140 (MQ=255)
                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTGCAAAATATTTCTCACAAGGTTTTTAACGGTTTTCTCACTGATAAACAAATATTCACCAATACCCCGGt                                                                                                 >  2:2522304/1‑140 (MQ=255)
                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCCTGCGTCCGGTCGTGGACAATCATTTTTTTGAAAAAATTTCTCACAAGGTTTTTGACCGTTTTCTCACAGATAAACAAAGAATAACCAAAACCCCGGt                                                                                                 >  1:1427728/1‑140 (MQ=255)
                                              tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTAATTTTTTGCAAAATAATGCTCACATGGTTTTTAACGGTTTTGTCAATGATAAACAATAATTCAACACAACCGCGGTTGCtt                                                                                            >  1:684536/1‑140 (MQ=255)
                                                tCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATAAATTTTTGCAAAAAAATGCTCACATGGTTTTTCACGGTTTTGACAATTATAAACAATTAATCACCAATAACGCCGTTGAtttt                                                                                          >  2:1125660/1‑140 (MQ=255)
                                                     ccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGt                                                                                     <  1:2203529/140‑1 (MQ=255)
                                                       aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTGAAAATAATTCTCACATGGTTTTTCACGGGTTTCTCACTGATAAACAAAGATTAACCAATACCGCGGGTGGTTTTTGCGTCt                                                                                   >  1:1828447/1‑140 (MQ=255)
                                                       aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTGCAAAATATTGCTCAAATGGTTTTTCAAGGTTTTCTAACAGATAAAAAAAGAATCACCAAAACCGCCGTTGCTTTTTACGtat                                                                                   >  2:3539155/1‑138 (MQ=255)
                                                        gCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTg                                                                                  <  1:1960679/140‑1 (MQ=255)
                                                        gCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTg                                                                                  <  2:1441179/140‑1 (MQ=255)
                                                         ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCTTTTACAATCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTATAACTGATAAACAATGATTAACCAATACCGCCGTTTCTTTTTCCGTCTGc                                                                                 >  2:1578422/1‑140 (MQ=255)
                                                             ttttAATGGCGACCACAACGGCGTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCCCGGGTTTCTCACTGATAAACAATTATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                             <  1:1118514/140‑1 (MQ=255)
                                                                  aTGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTg                                                                        <  1:1194845/140‑1 (MQ=255)
                                                                   tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTGCAAAAAATTGCTCACATTGTTTTTCACGGTTTTCTCACTGATAAACAATGTTTGACCAATACCGCGGTTGGTTTTTCCGTATTCACTCATCTgc                                                                       >  2:2684792/1‑140 (MQ=255)
                                                                   tGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATAATGCTCACATGGTTTTTCACGGTTTTCTCAATGATAAACAATTATTCACCAAAAACCCGGTTGCTTTTTGCGTCTGCAAGCATCttc                                                                       >  2:2426987/1‑138 (MQ=255)
                                                                     gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                     <  2:3472372/140‑1 (MQ=255)
                                                                     gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                     <  1:1147574/140‑1 (MQ=255)
                                                                     gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                     <  1:3388685/140‑1 (MQ=255)
                                                                     gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                     <  1:2194809/140‑1 (MQ=255)
                                                                     gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                     <  1:2273820/140‑1 (MQ=255)
                                                                       gACCACAACGGCTTGCGTCCGGTCGTTTACATTCCTTTTTTGCAAAAAAATGCTCACATGGTTTTTCACGGTTTTGTCACTGATAAACAAAGATTCACCAATACCCCGGTTGCTTTTTCCGGCGGCAAGCAGCTgcagca                                                                   >  2:628443/1‑140 (MQ=255)
                                                                       gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                                                                   <  1:3140270/140‑1 (MQ=255)
                                                                                   ttGCGTCCGGTCGTTTAAATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACCGTTTTCTCACTGATAAACAATGATTAACCAAAACCGCGGTTTCTTTTTCCGGCGGCACGCAGCTGCAGCACATaacagtacc                                                       >  2:83503/1‑135 (MQ=255)
                                                                                    tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                                      <  2:2903816/140‑1 (MQ=255)
                                                                                           ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                                               <  2:2079270/140‑1 (MQ=255)
                                                                                           ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                                               <  2:569300/140‑1 (MQ=255)
                                                                                            gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                                              <  2:133079/140‑1 (MQ=255)
                                                                                            gTCGTTTAAATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                                              <  1:1904979/140‑1 (MQ=255)
                                                                                             tCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaa                                             <  2:3666589/140‑1 (MQ=255)
                                                                                              cgattACATTCATTTTTTGCAAAATATTGCTCACAGGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCCTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  2:1039278/137‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  1:1723592/140‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  1:1990869/140‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  1:2522304/140‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  1:1287374/140‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  1:3460291/140‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTGTGCAAAATATTGCTCACATGGGTTTGCACGGTTTTCTCACTGATAAACACTGATTCACCAAGACCGCGGTTGCTTTTGCCGTCGGCAAGCCTCTGCACCACTTCACATGCCCGCCGAGTTaac                                            <  2:3028873/140‑2 (MQ=255)
                                                                                                tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                          <  2:1532256/140‑1 (MQ=255)
                                                                                                tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                          <  1:1003664/140‑1 (MQ=255)
                                                                                                tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                          <  1:2397988/140‑1 (MQ=255)
                                                                                                     attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa                                     <  1:2913664/140‑1 (MQ=255)
                                                                                                             tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTg                             <  1:189093/140‑1 (MQ=255)
                                                                                                             tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTg                             <  1:2708084/140‑1 (MQ=255)
                                                                                                             tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTg                             <  1:3102301/140‑1 (MQ=255)
                                                                                                               tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                           <  1:828700/140‑1 (MQ=255)
                                                                                                               tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                           <  1:3554448/140‑1 (MQ=255)
                                                                                                               tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                           <  1:3539155/140‑1 (MQ=255)
                                                                                                               tGCAAAATATTGCTCACATGGTTTGTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                           <  2:3067798/140‑1 (MQ=255)
                                                                                                                    aaTATTGCTCACCTGGTTTTTCACGGTGTTCTCACTGATACACACTGATTCACCAATACCGCGGTTGCTTTTGCCCTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                      <  1:3086434/140‑1 (MQ=255)
                                                                                                                    aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                      <  1:447631/140‑1 (MQ=255)
                                                                                                                         tGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGa                 >  1:1560609/1‑140 (MQ=255)
                                                                                                                          gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                <  2:1822299/140‑1 (MQ=255)
                                                                                                                          gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                <  2:2383231/140‑1 (MQ=255)
                                                                                                                            tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACCTTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg              <  2:3809741/140‑1 (MQ=255)
                                                                                                                            tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg              <  2:1187887/140‑1 (MQ=255)
                                                                                                                              acaTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAACAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGAc            <  1:883029/140‑1 (MQ=255)
                                                                                                                                ctGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  2:3768876/139‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCTCATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          >  1:3712827/1‑140 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          >  2:1083699/1‑140 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  2:2192720/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          >  2:736976/1‑140 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          >  1:1602599/1‑140 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          >  1:2637215/1‑140 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          >  1:3484443/1‑140 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          >  1:3731129/1‑140 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  2:874567/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  2:797965/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  1:3289773/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTATCACTGATAAACAATGATTCACCAATACAGCTGTTGCTTTTTCCGTCTGCACGCATCTGCAGCCCTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAGTTCAGGATAGTCCt          >  1:3006687/1‑140 (MQ=255)
                                                                                                                                       ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg   <  1:213854/140‑1 (MQ=255)
                                                                                                                                       ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg   <  1:2080480/140‑1 (MQ=255)
                                                                                                                                       ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg   <  2:1635757/140‑1 (MQ=255)
                                                                                                                                       ttCACGGTTTTCTCACTGATAAACAATGATTCACCAAGACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg   <  1:3797060/140‑1 (MQ=255)
                                                                                                                                        tCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGa  <  2:1306387/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
AAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGA  >  NZ_CP034943/3446858‑3447133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: