Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,562,015 A→C 11.2% intergenic (‑661/‑137) EO946_RS18300 ← / → EO946_RS21105 response regulator/HAMP domain‑containing histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,562,0150AC11.2% 552.8 / 2.9 238intergenic (‑661/‑137)EO946_RS18300/EO946_RS21105response regulator/HAMP domain‑containing histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (127/65);  new base C (2/40);  total (129/109)
Fisher's exact test for biased strand distribution p-value = 2.99e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

ATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATCA  >  NZ_CP034943/3561879‑3562152
                                                                                                                                        |                                                                                                                                         
aTCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAggg                                                                                                                                        <  1:2886796/140‑1 (MQ=255)
 tCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGt                                                                                                                                       <  1:2652633/140‑1 (MQ=255)
     cACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATAAACGAGCTGTTTTACGCTTCACCAGGGTGACa                                                                                                                                   >  2:3152359/1‑140 (MQ=255)
          cgtTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGaa                                                                                                                              >  1:3655548/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                  >  1:352262/1‑140 (MQ=255)
                      gTGCAAATCGCATCACTTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                  >  1:2805571/1‑140 (MQ=255)
                       tGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCATATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAAt                                                                                                                 >  1:3494048/1‑140 (MQ=255)
                       tGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGt                                                                                                                                       >  1:2977373/1‑118 (MQ=255)
                       tGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGt                                                                                                                                       <  2:2977373/118‑1 (MQ=255)
                       tGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAAt                                                                                                                 >  1:640612/1‑140 (MQ=255)
                       tGCAAATCGCATCTATTTTAATGGCCAAGTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGAGTTTATAGCGCAAAACAGACCATATACGCGCTTTTTTACGGTTCACAAGGGTGACAATGACCTTGTGGTACact                                                                                                                 >  1:3008821/1‑138 (MQ=255)
                        gCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAAtt                                                                                                                >  1:1641254/1‑140 (MQ=255)
                         cAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAAttt                                                                                                               >  2:1418171/1‑140 (MQ=255)
                         cAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAAttt                                                                                                               >  2:2704725/1‑140 (MQ=255)
                              cGCATCTATTTTAATGTCCAATTAATAGGACAAAGCGGTAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTAAGCTTCACAAGGGTGACAATGAACTAGTGGTACAATATTCaat                                                                                                          >  2:1869463/1‑139 (MQ=255)
                                 aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACtt                                                                                                       >  1:396877/1‑140 (MQ=255)
                                       tttAATGTCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACACACCATATACGAGCTTTTTTACGCTACACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGCaaa                                                                                                 <  2:1950905/140‑1 (MQ=255)
                                           aTGGCCAATTAATCGCAAAACGCGGAAGCCCTTCAAATAAACCTTATTTTATAGCCCAACACAGCCCATATACCAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACg                                                                                             <  2:1130427/140‑1 (MQ=255)
                                              gCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAAAGCCg                                                                                          >  2:2868737/1‑140 (MQ=255)
                                              gCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTAAGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCg                                                                                          >  2:622615/1‑140 (MQ=255)
                                                      attgcaCAAAGCGGAAGCCCTCAAAAAAAACCTGATTTTATAGCGCAAAACACACCATATACGACCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTgggg                                                                                  <  2:2278081/135‑1 (MQ=255)
                                                      aTAGGACAAAGCGCAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTACAAACTTCGGAAACACGCCGGGGTgggg                                                                                  <  2:310162/140‑1 (MQ=255)
                                                      aTAGCAAAAAGCGGAACCCCTTAAAATAAACCTGATCTTAGAGCCAAAAACACACCAGATACGAGCTTTATTACGCTTCCCAAGGGTGACAATCAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTgggg                                                                                  <  1:1524508/140‑1 (MQ=255)
                                                        aGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGaa                                                                                >  1:311030/1‑140 (MQ=255)
                                                           aCAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCg                                                                             >  2:2760340/1‑140 (MQ=255)
                                                             aacgCGGAAGCCATTCAAAAAAACATGATTTTATAGCGCAAAACACCCAATATACGCGCTTTTTTCCGCTTCACCCGGGTGACAATGAACTTGTGCTACAATTTTCAACCTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                           <  2:1815775/137‑1 (MQ=255)
                                                             aaaGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                           >  1:2733431/1‑140 (MQ=255)
                                                             aaaGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACCAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCAGGGGTGGGGAATCGCa                                                                           >  2:2444295/1‑140 (MQ=255)
                                                             aaaGCGGAAGCCCTTAAAATAAACCTGATTTTATCGCGCAAAACACACCATATACGAGCTTTTTTACGCTTCACACGGGGGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                           <  2:3257184/140‑1 (MQ=255)
                                                             aaaGCGGAAGCCCTACAAATAAACCTGATTTTATAGCGCAAAACAGACCATATCCGAGATTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                           <  2:2141616/140‑1 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                         >  2:3552649/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                         >  1:1040754/1‑140 (MQ=255)
                                                                    acgCCCTTCAAATAAACGTGATTTTATAGCGCACAACATACCATATACGAGCTTTTTGACGCTTCGCAAGGGTGACAATGAACTTGTGGTAGAATTTTCAATCTTCGGAAAAACGCCGGGGTGGGGAATCGGAATGCCGa                                                                    >  2:248006/3‑140 (MQ=255)
                                                                    aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGa                                                                    >  1:2008047/1‑140 (MQ=255)
                                                                    aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGa                                                                    >  2:1054930/1‑140 (MQ=255)
                                                                    aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGa                                                                    >  2:3320750/1‑140 (MQ=255)
                                                                     atcacTTCAAATAAACCTGATTTTATCGCGCAAACCACACCATCTACTAGCTTTTTTACTCTTCACACGGTTGACAATCAACTTGTGGTACATTTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                                                   <  1:3477380/136‑1 (MQ=255)
                                                                     aGCCCTAAAAATAAACTTTTTTTTATAGCGCAAAACCCCCCATTTACGAGATTTTTTACGCTTCAAACGGGTCAAATTTAATTTGTGTTACATTTTACAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                                                   <  1:1870226/140‑1 (MQ=255)
                                                                     aGCCATTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATCAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                                                   <  2:2746519/140‑1 (MQ=255)
                                                                        ccTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTAc                                                                <  2:834123/140‑1 (MQ=255)
                                                                           tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGTGTGGGGAATCGCAAAGCCGATTACttt                                                             >  1:1026497/1‑140 (MQ=255)
                                                                           tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                                                             >  1:719839/1‑140 (MQ=255)
                                                                           tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACtgt                                                             >  2:702041/1‑138 (MQ=255)
                                                                                tAAACCTGATTTTTTTGCGCAAAACAGACCTTATACTAGCTTTTTTACGCTTCACAAGGGTTACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGaa                                                        <  2:1168293/140‑1 (MQ=255)
                                                                                tAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGaa                                                        <  2:667972/140‑1 (MQ=255)
                                                                                tAAACCTGATTTTATAGCGCAAAACACACCAAATCCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGGGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGaa                                                        <  2:1654054/140‑1 (MQ=255)
                                                                                tAAACATGATTTTATAGCCAAAAACCGACCATATCCGAGCTTTTTTACGCTTCACAAGGGTGAAAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGaa                                                        <  2:3207526/140‑1 (MQ=255)
                                                                                aaaaCCTGTTTTTATAGCCCAAAACACACCATATACGAGTTTTTTTCCGTGTCACATGGTTTACATTTAATTTGTGTTAAAATTTCCAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGaa                                                        <  1:2334649/139‑1 (MQ=255)
                                                                                  aacgttaTTTTATAGCGCAAAACAGCCCCTATCCGAGCTTTTTTCCGCTTCACACGGGTCACAATCAACTGGGGGTACAATTTTCAAACTGCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  1:2610603/134‑1 (MQ=255)
                                                                                  aacattaTTTTATAGCCCAAACCACACCATATACGAGCTTTTTTCCGCTTCACACGGGTGACAATTAACTTGTGGTAAATTTTTCAAACTGCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  1:1083302/134‑1 (MQ=255)
                                                                                  aaCCTTATTTTATCGCGCAAAACAGACCATATACGTGCTTTTTTACGATACACATGGGTGACATTGAAGTTTTTTTACAATTTACAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  1:1104999/140‑1 (MQ=255)
                                                                                  aaCCTTATTTTATAGCCCAAAACAGACCATATCCGAGCTTTTTTACGCTTCACACGGGTGAAAATGAACTGGTGGTACAATTTAAAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  1:178145/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATCGCGCAAAACCGACCATATCCGCGCTTTTTTCCGCTTCACAAGGGTCACAATGAAGTGGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  1:1157000/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCGCAAAACAGACCATATCCGCGCTTTTTTACGCTTCCCACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  2:241218/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCGCAAAACAGACCATATCCGAGCTTTTTTACGCTTCACAAGGGTGACAATTAACTTGTGGTACATTTTTCAAAGTTGGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  2:1990606/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACATTTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  2:1381335/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCGCAAAACACCCCCTATCCGAGCTTTTTTACGCTTCACACGGGTTACAATGACCTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  2:3674807/140‑1 (MQ=255)
                                                                                       gATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTAGTGTTGGAACGCAgca                                                 >  1:1560050/1‑138 (MQ=255)
                                                                                       gATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCCCAAAGCCGATTACTTTTGGAACGCAGGa                                                 >  2:3332975/1‑140 (MQ=255)
                                                                                        aTTTTATTGCCCAAAACATACCATATCCGAGCTTTTTTACGTTTCACCCGGGTGAAATTCAACTTGTGTTACAATTTACAAACTTCGGAAACACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                                <  1:1836191/140‑1 (MQ=255)
                                                                                        aTTTTATCCCGCAAAACCCACCATATACGAGATTTTTAACGCTTCACACGGGTGCCAATGAACTTGTGGTACAATTTCCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                                <  1:3437952/140‑1 (MQ=255)
                                                                                        aTTTTATAGCGCAACACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACATTTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                                <  2:2650321/140‑1 (MQ=255)
                                                                                        aTTTTATAGCGCAAAACATCCCTTATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                                <  2:164073/140‑1 (MQ=255)
                                                                                        aTTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      <  2:654023/134‑1 (MQ=255)
                                                                                        aTTTTATAGCGCAAAACAGACCATACACGAGCTTTTTTACGCTTCCCCAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                                <  2:757578/140‑1 (MQ=255)
                                                                                          ttaagaGCGCAACACACACCAGACACGAGCTTTTTTACGCTTCACAAGCCTGACAATGAACTTGTGGTACAATTTTAAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa                                              <  2:12072/135‑1 (MQ=255)
                                                                                             ataGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACat                                           >  2:1756469/1‑140 (MQ=255)
                                                                                             ataGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACat                                           >  2:41917/1‑140 (MQ=255)
                                                                                             ataGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACat                                           >  2:3630183/1‑140 (MQ=255)
                                                                                               aGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                                                   >  1:1698110/1‑114 (MQ=255)
                                                                                                  gcAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGc                                      >  1:209981/1‑140 (MQ=255)
                                                                                                  gcAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGc                                      >  1:1755306/1‑140 (MQ=255)
                                                                                                  gcAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGc                                      >  1:210696/1‑140 (MQ=255)
                                                                                                   cAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTCCAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTATTGGAACGCAGGATAACATATTGCg                                     >  2:1279223/1‑140 (MQ=255)
                                                                                                   cAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCg                                     >  2:45221/1‑140 (MQ=255)
                                                                                                   cAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAGTAGTTTTGGAACGCAGGATAACATATTGCg                                     >  1:1301393/1‑140 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGCTTCACAAGTGTGACAATGAACTTGTGGTCCAATATTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATAGCGAGAGAAcc                             >  2:2229567/1‑139 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGACCa                             >  1:2639938/1‑140 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                             >  2:2038012/1‑140 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                             >  1:3776725/1‑140 (MQ=255)
                                                                                                                ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAACAtt                        >  1:3059955/1‑140 (MQ=255)
                                                                                                                ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAAtt                        >  2:3756596/1‑140 (MQ=255)
                                                                                                                ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAAtt                        >  1:2701908/1‑140 (MQ=255)
                                                                                                                    gAGCTTTTTTACGCTTCACAAGGTTGACAATGAACTTGTGGTAAAATTTTCAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCt                    <  2:3408445/140‑1 (MQ=255)
                                                                                                                     aGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACACAATTTCCtt                   >  1:2280502/1‑140 (MQ=255)
                                                                                                                     aGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCtt                   >  1:2505507/1‑140 (MQ=255)
                                                                                                                     aGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCtt                   >  1:797494/1‑140 (MQ=255)
                                                                                                                       cTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCCATTACTTTTGCAACTCAGGATAACATATTGCGAGAGAACAAAAATTCCTTTc                 >  2:2302220/1‑140 (MQ=255)
                                                                                                                        ttttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCt                >  1:440507/1‑140 (MQ=255)
                                                                                                                         tttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCATACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCtt               >  2:784249/1‑140 (MQ=255)
                                                                                                                         tttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCtt               >  1:2938179/1‑140 (MQ=255)
                                                                                                                         tttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCtt               >  1:584036/1‑140 (MQ=255)
                                                                                                                          ttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTa              >  1:3167058/1‑140 (MQ=255)
                                                                                                                          ttttACGCTTCACAAGGGTGAAAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTa              <  2:495577/140‑1 (MQ=255)
                                                                                                                                ggttCACACGGGTGAAAATTAACTTGTGGTACAATTTTCAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGAtt        <  2:2321138/138‑1 (MQ=255)
                                                                                                                                    cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGGTTACTTTTGGAACg                                                      >  1:1681632/1‑90 (MQ=255)
                                                                                                                                    cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                      >  1:2473453/1‑90 (MQ=255)
                                                                                                                                    cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGactgtct    >  1:1914442/1‑134 (MQ=255)
                                                                                                                                    cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt    >  1:2329634/1‑140 (MQ=255)
                                                                                                                                      caAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATCa  <  2:2598373/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
ATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATCA  >  NZ_CP034943/3561879‑3562152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: