BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,843,776	C→G	7.4%	V161L (GTT→CTT)	EO946_RS19775 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,843,776	0	C	G	7.4%	746.3 / 6.9	259	V161L (GTT→CTT)	EO946_RS19775	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (161/71); new base G (2/21); total (164/95)
Fisher's exact test for biased strand distribution p-value = 1.26e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

CGTTCAAATGATGCCTTGTCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTT  >  NZ_CP034943/3843637‑3843915

cGTTCAAATGATGCCTTGTCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAAc                                                                                                                                             >  1:1307976/1‑140 (MQ=255)

 gTTCAAATGAGGCCTTGTCAGCCCCCTGCTTCTTGACACTGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCGTGTCTTCTTCGCGTTTTTCGGTGTAACt                                                                                                                                            <  1:2662649/140‑1 (MQ=255)

         gatgCCTTGTCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCtgat                                                                                                                                    <  2:1904929/140‑1 (MQ=255)

            gCCTTGTCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAg                                                                                                                                 <  2:2874848/140‑1 (MQ=255)

             ccTTGTCAGCCCCGTGCTTCTTGACATTGCTGTTCATGATTTTGTCCCTTTCAGCTCTGTATTGCGTATATTCTTTTTTCAGTTGATCAAGCTGCTGTCTTCTCTTCTTCGCGTTTTTCGTTTTAACTTGGCTGATGAGt                                                                                                                                <  1:101396/140‑1 (MQ=255)

              cTTGTCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGtt                                                                                                                               <  2:3320992/140‑1 (MQ=255)

                tGTCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGATGTATTGCGTATATTGTTTTTTCCGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTg                                                                                                                             <  2:5007/140‑1 (MQ=255)

                 gTCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTCACTTGGCTGATGAGTGTGa                                                                                                                            >  1:2227311/1‑140 (MQ=255)

                 gTCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGa                                                                                                                            >  1:3641047/1‑140 (MQ=255)

                 gTCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTGTTTCGGTGTAACTTGGCTGATGAGTTTGa                                                                                                                            >  1:3555299/1‑140 (MQ=255)

                  tCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGAt                                                                                                                           <  2:2012701/140‑1 (MQ=255)

                  tCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGAt                                                                                                                           <  1:3047636/140‑1 (MQ=255)

                  tCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGAt                                                                                                                           <  2:1173499/140‑1 (MQ=255)

                    aGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCa                                                                                                                         <  1:681272/140‑1 (MQ=255)

                      cccccTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATg                                                                                                                       >  1:438692/1‑140 (MQ=255)

                       ccccTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCactg                                                                                                                      <  1:1476873/140‑4 (MQ=255)

                       ccccTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCactg                                                                                                                      <  1:3467129/140‑4 (MQ=255)

                         ccTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCtt                                                                                                                    >  2:3503430/1‑140 (MQ=255)

                         ccTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCtt                                                                                                                    >  2:3692021/1‑140 (MQ=255)

                          cTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTGGGTGTAAGTTGGCTGATGAGTTTGATCATGGttg                                                                                                                   >  2:3796666/1‑139 (MQ=255)

                          cTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATGGCGTATATTGTTTTGTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTGTGATCATGCttt                                                                                                                   >  1:1894754/1‑140 (MQ=255)

                              ttcttGACACTGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCtgt                                                                                                               <  2:2740856/140‑1 (MQ=255)

                              ttcttGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCtgt                                                                                                               <  2:832138/140‑1 (MQ=255)

                                cttGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGa                                                                                                             >  1:1112723/1‑140 (MQ=255)

                                cttGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGa                                                                                                             >  2:90964/1‑140 (MQ=255)

                                cttGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGa                                                                                                             >  1:1567026/1‑140 (MQ=255)

                                       aTGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCaaa                                                                                                      >  1:3676872/1‑140 (MQ=255)

                                        tGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCaaaa                                                                                                     >  1:2743288/1‑140 (MQ=255)

                                        tGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCaaaa                                                                                                     >  2:1136331/1‑140 (MQ=255)

                                        tGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCaaaa                                                                                                     >  2:1613558/1‑140 (MQ=255)

                                        tGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCaaaa                                                                                                     >  1:2547507/1‑140 (MQ=255)

                                            ttacATGATTTTGCCGCTTACCGCGGTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCTTTTTTCGGTGTAAGTTGGCTGATGATTTTGATAATGATTTCTGTGACCTAAAAAAAAg                                                                                                 <  2:1428747/137‑1 (MQ=255)

                                             ttCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGc                                                                                                >  1:311308/1‑140 (MQ=255)

                                             ttCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGc                                                                                                >  1:2082314/1‑140 (MQ=255)

                                             ttCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGc                                                                                                >  1:13548/1‑140 (MQ=255)

                                             ttCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCATCTTCGCGTTTTTCGGTGTAACTTGTCTGATGAGTTTGATCATGATTTATGTGACCTCAAAAAAAGc                                                                                                >  2:3034579/1‑140 (MQ=255)

                                             ttCAGGATTTTGACGCTTTCAGCGCGGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTTTGAACTAAAAAAAAGc                                                                                                >  2:584898/1‑140 (MQ=255)

                                               attGATTTTGACGCTTTCAGCGCTTTTTTGCGTATTTTGTTTTTTCAGTTGATCAAGCTGCTTTGTTGTCTTCTTGGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTAAAAAAAAGCgg                                                                                              <  2:2772277/138‑1 (MQ=255)

                                                     tttGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTTATCAAGCTGCTGTCTTTTCTTCTTCGCTTTTTTGGGTGTAACTTGGCTTATGAGTTTGATCATGATTTCTGTGACCTAAAAAAAAGCGGCTCTAt                                                                                        <  2:1865070/140‑1 (MQ=255)

                                                       tGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACt                                                                                                                                            <  2:3641572/86‑1 (MQ=255)

                                                       tGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTAttg                                                                                      >  1:936962/1‑139 (MQ=255)

                                                          ggttttCCGCGCTTTTTTGCTTATTTTTTTTTTTCAGTTTAACACGCTGCTGTCTTGTCTTTTTCGCTTTTTTGGGTGTACGTTGGCTGTTGTTTTTTATCATGATTTCTGTGCCCCAAAAAAAAGCGGCTCTATtttct                                                                                   <  1:964992/137‑1 (MQ=255)

                                                          cGCTTTCTGCGTTTTATTGCTTATATTGTTTTTTCAGTTGATAAAGGTGGTGTCTTGTCTTATTCGTGTTTTTCGGTGTAACTTGGCTTATGAGTTTTATCATGCTTTGTGTGACCTCAAAAAAAGCGGCTCTATtttct                                                                                   <  2:3527114/140‑1 (MQ=255)

                                                          cGCTTACAGCGGTTTATTGCTTATTTTTTTTTTTCATTTGATCACCCTGCTGTCTTGTCTTATTCGCGTTTTTCGGTTTAAGTTGGCTTTTGATTTTGATATGGCTTTCGGTCACCAAAAAAAAAGCGGCTCTATtttct                                                                                   <  1:1937040/140‑1 (MQ=255)

                                                              ttCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCa                                                                               >  2:1505188/1‑140 (MQ=255)

                                                               tCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAg                                                                              >  1:2380224/1‑140 (MQ=255)

                                                                            gcttataTTTTTTTTTCAGTTGATCAAGCTGTTGTCTTGTCTACTTCGCTTTTTTGGGTGTAACTTGGCTGATGATTTTTATCATGCTTTCTGTCACAAAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCg                                                                 <  1:3415404/137‑1 (MQ=255)

                                                                             cGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGt                                                                >  2:3180042/1‑140 (MQ=255)

                                                                               tcttttGTTTTTTCAGTGGATCAAGCTGCTGTCTTGTCGTCTTCGCGTTTTTCGGTGTAACTGGGCTGATGTGTTTGATCATGCTTTCGGTGACCACAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTg                                                              <  2:2127893/136‑1 (MQ=255)

                                                                               tataTTGTTTTTTCAGTTGATCAATCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATAATGGTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTg                                                              >  2:2652219/1‑140 (MQ=255)

                                                                               tataTTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACt                                                                                                                                            <  1:1875734/62‑1 (MQ=255)

                                                                               tataTTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTg                                                              >  1:3228593/1‑140 (MQ=255)

                                                                                  atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTg                                                              >  1:748365/1‑137 (MQ=255)

                                                                                  atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                           >  1:2449371/1‑140 (MQ=255)

                                                                                  atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                           >  1:280233/1‑140 (MQ=255)

                                                                                  atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                           >  1:2861587/1‑140 (MQ=255)

                                                                                  atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                           >  1:3078958/1‑140 (MQ=255)

                                                                                  atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                           >  1:833524/1‑140 (MQ=255)

                                                                                  atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                           >  2:1261822/1‑140 (MQ=255)

                                                                                  atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                           >  1:636492/1‑140 (MQ=255)

                                                                                  atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                           >  2:3267743/1‑140 (MQ=255)

                                                                                  atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCACAAAAAAAGCGGCTCTATTTTCTTTCCTCTGTTGCATTTCGTTGCgg                                                           >  2:2821557/1‑140 (MQ=255)

                                                                                  atTGTTTTTTCAGTTGATCAAGCTGCGGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCAGTTCGTTGCgg                                                           >  1:3718276/1‑140 (MQ=255)

                                                                                    ttttttttCAGTTGATCACGCTGCTGTCTTGTCTTCTGCGCTTTTTTCGGTTTACGTTGGCTGATGTTTTTTAACATGTTTTATGTGACCAAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCt                                                         <  1:3197835/138‑1 (MQ=255)

                                                                                    tGTTTTTTCAGTTTATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTAGGTGTAACTTGGCTGATGAGTTTGATAATGATTTATTATAACAAAAAAAAAGCGGATCTATATTCTTACATCTAATGAATTTCGTTGCggat                                                         >  2:199369/1‑138 (MQ=255)

                                                                                     tttttttCAGTTGATCACGGTGCTGTTTTGTCTTCTTCGGGTTTTTCGGTGTAAGTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTc                                                        <  2:2501395/139‑1 (MQ=255)

                                                                                      ttttttCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCa                                                       >  2:272931/1‑140 (MQ=255)

                                                                                          ttcctttGATCAAGCTGCTGTTTTGTCTTCTTCGCGTTTTTCGGTTTAACTTGGCTGATGAGTTTGATCATGCTTTTTGTCACCAAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTc                                                   <  1:1613558/135‑1 (MQ=255)

                                                                                          ttCCGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTGCGGTGTAAGTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTc                                                   <  2:2104756/140‑1 (MQ=255)

                                                                                           tCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCt                                                  >  2:205995/1‑140 (MQ=255)

                                                                                                  aTCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTAc                                           >  1:1693354/1‑140 (MQ=255)

                                                                                                    cAAGCTGGTGTCTTGTCTTCTTCGCGTTTTGGGTTGTAAGTTGGCTGTTGTGTTTGATCTTGCTTTCTGTGACATCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACgg                                         <  1:2741619/140‑1 (MQ=255)

                                                                                                    aaaGTTGCTGTCTTTTGTTGTTCGGTTTTTTGGGTTTAAGTTGGCTGTTGATTTTGATCATGGTTTCTGTGACCTAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACgg                                         <  1:479178/139‑1 (MQ=255)

                                                                                                     aaGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACggg                                        <  2:2861587/140‑1 (MQ=255)

                                                                                                         tgctgTCTTGTCTTCTGCGTTTTTTTCGGTGTAAGTTGGCTGATGTTTTTTATATTGGTTTCTGTGACCAAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGtttt                                    <  2:1693354/140‑1 (MQ=255)

                                                                                                          ggtgtgttgtctTCTTGGTTTTTTTCGGTGAAACTTGGCTTATGATTTTGATCATGTTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGttttt                                   <  1:446541/138‑1 (MQ=255)

                                                                                                          gctgTCTTGTCTTCTTCGCGTTTTTCGGTGTAAGTTGGCTTATGATTTTGATCATGCTTTCTGTGACCTAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGttttt                                   <  1:2252522/140‑1 (MQ=255)

                                                                                                           ctgTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCCTTTCTTGATTACGGGtttttt                                  >  2:341542/1‑140 (MQ=255)

                                                                                                           ctgTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGtttttt                                  >  2:2364599/1‑140 (MQ=255)

                                                                                                           ctgTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGAGTACGGGggtttt                                  >  2:1131538/1‑140 (MQ=255)

                                                                                                               tttgtcttCTTCGCTTTTTTCGGTTTAACTTGGTTTATTATTTTGATCAGGGTTTCTGTGACCACAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAg                              <  1:77401/139‑1 (MQ=255)

                                                                                                                ttgtcttCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGt                             >  1:1237788/1‑140 (MQ=255)

                                                                                                                ttgtcttCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGt                             >  1:2297279/1‑140 (MQ=255)

                                                                                                                 tgtcttCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTATATTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTTCGGGTTTTTTGGAGTa                            >  1:3794396/1‑140 (MQ=255)

                                                                                                                  tttttcttcGCTTTTTTCGGTGTAATTTGGCTGTTGATTTTTATCATGCTTTCTGTCACCTAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTaa                           <  1:20460/137‑1 (MQ=255)

                                                                                                                   tcttctTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTaaa                          >  1:1174341/1‑140 (MQ=255)

                                                                                                                     ttcttcGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATGACGGGTTTTTTGGAGTAAAGc                        >  2:744759/1‑140 (MQ=255)

                                                                                                                     ttcttcGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGAGTACGGGTTTTGTGGAGTAAAGc                        >  2:245994/1‑140 (MQ=255)

                                                                                                                       cttcGCGTTTTTCGGTGTACCTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCaa                      <  2:802532/140‑1 (MQ=255)

                                                                                                                       cttcGCGTTTTGCGGTGTAAGTTGGCTGATGAGTTTGATCATGCTTTCTTTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCaa                      <  2:3563619/140‑1 (MQ=255)

                                                                                                                        ttcGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTTATTACGGGTTTTTTGGAGTAAAGCAAc                     >  1:2171755/1‑140 (MQ=255)

                                                                                                                        ttcGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAAc                     >  2:110114/1‑140 (MQ=255)

                                                                                                                        ttcGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAAc                     >  1:2934951/1‑140 (MQ=255)

                                                                                                                          cgcgTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCAttt                                                                   >  1:2626842/1‑92 (MQ=255)

                                                                                                                          cgcgTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCAttt                                                                   <  2:2626842/92‑1 (MQ=255)

                                                                                                                          cgcgTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTg                   >  2:2997798/1‑140 (MQ=255)

                                                                                                                             gTTTGTCGGTGTAACTTGGCGGCTGTGTTTCATCATGCTTCCTGCCACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCt                <  1:3067701/140‑1 (MQ=255)

                                                                                                                               ttttCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCg              <  2:1336016/140‑1 (MQ=255)

                                                                                                                               ttgtCGGGGTAACTGGGCTGATGAGTTTGACCATGCTTTCGGGGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCg              <  1:1361550/137‑1 (MQ=255)

                                                                                                                                           cTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCgtt  >  2:2316401/1‑140 (MQ=255)

CGTTCAAATGATGCCTTGTCAGCCCCCTGCTTCTTGACAATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTT  >  NZ_CP034943/3843637‑3843915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`