BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,198	G→T	14.9%	Q112K (CAA→AAA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,198	0	G	T	14.9%	587.0 / 32.7	304	Q112K (CAA→AAA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (116/132); new base T (53/2); total (169/135)
Fisher's exact test for biased strand distribution p-value = 2.41e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

GTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAT  >  NZ_CP034943/240059‑240337

gtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCGGATGGTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATACCGACTATACGCGCGTTTTCGGATTTTTGTTTGAAATTTTg                                                                                                                                             >  1:2932769/1‑140 (MQ=255)

 tggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTgg                                                                                                                                            <  1:2764085/140‑1 (MQ=255)

         gtgtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTg                                                                                                                                    >  2:1291953/1‑140 (MQ=255)

           gtAAGAGAGTGAGAAGGCGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                                  <  2:534394/140‑1 (MQ=255)

           gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGTATTTTTGGGAGATTTGaa                                                                                                                                  >  2:2887929/1‑140 (MQ=255)

           gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                                  <  2:3469397/140‑1 (MQ=255)

              agagagagagAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGATTTTGGAAAATTTAAtag                                                                                                                               >  1:2036814/1‑138 (MQ=255)

              agagagagagAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTTAAttg                                                                                                                               >  2:3098689/1‑138 (MQ=255)

                   gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAATTTTTGGGATATTTTAATTGCtttt                                                                                                                          >  1:3424589/1‑140 (MQ=255)

                   gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCtttt                                                                                                                          >  2:3150114/1‑140 (MQ=255)

                   gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGGTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATTGCtttt                                                                                                                          >  2:161783/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTc                                                                                                                         >  2:663407/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTGTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCtttta                                                                                                                         >  1:724156/1‑139 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTGTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTTAATTTTTTGGAGATTTGAATGGCTTTTc                                                                                                                         >  1:3282093/1‑140 (MQ=255)

                      agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGGTTTTGCg                                                                                                                       >  1:3333630/1‑140 (MQ=255)

                          ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGTATTTTTTTGAGATTTGAATTGCTTTTCCGtttt                                                                                                                   >  2:3539490/1‑140 (MQ=255)

                          ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATTGCTTTTACGtttt                                                                                                                   >  1:1115854/1‑140 (MQ=255)

                                tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAAc                                                                                                             >  2:2523106/1‑140 (MQ=255)

                                tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTAAGGTTTTGGTGATTTTAAAGGCTTTTACGGTTTTAAAAc                                                                                                             >  1:544103/1‑140 (MQ=255)

                                  tctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCCAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTCCGTTTTTAAAACaa                                                                                                           >  2:3367445/1‑140 (MQ=255)

                                  tcggATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGCCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGTATGGCTGGTACGTTTTTAAAACaa                                                                                                           >  1:3616226/4‑140 (MQ=255)

                                  tcggATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGCCGTTTTGGGATTTTTGTTTTAAGTTTTTGGATATTTGAATGGGTTTTACGTTTTTAaaaaaa                                                                                                           >  1:3789736/4‑140 (MQ=255)

                                   ctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTTAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACaat                                                                                                          >  2:2381613/1‑139 (MQ=255)

                                   ctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCGTTTCCGTTTTTAAAACAag                                                                                                          >  2:1934825/1‑140 (MQ=255)

                                      aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTTAGTTTTTGGAGATTTTAATGGGTTTTGCGTTTTTAAAAAAAGAgg                                                                                                       >  1:138522/1‑140 (MQ=255)

                                      aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTGTTCCGTTTTTAAAACAAGAgg                                                                                                       <  1:586993/140‑1 (MQ=255)

                                       tGTTTGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGc                                                                                                      <  2:239872/140‑1 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                   <  1:3741957/140‑1 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                   <  2:935693/140‑1 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTTTTTTTAGTTTTTGGAGAATTTTATTGGTTTTACGTTTTTAAAACAAAAGGCaacc                                                                                                  >  1:2325513/1‑138 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAATTTTTGGAGATTTTAATTGCTTTTACGTTTTTAAAACAAGAGGCaatc                                                                                                  >  1:49255/1‑138 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAATTTTTTGGAGATTTTAATTGCTTTTACGTTTTTAAAACAAAATGCAAGc                                                                                                  >  2:120114/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                  >  2:103261/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTTAATGGCTTTTACGTTTTTAAAACAAAATGCAAGc                                                                                                  >  2:1091602/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAATAGAATAGGCAAGc                                                                                                  >  2:2324750/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGGTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAATAGGCAAGc                                                                                                  >  2:1896459/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATGGCTTTTACGGTTTTTAAAAAATAGGCAAGc                                                                                                  >  1:2575712/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAAAAAGAGGCAAGc                                                                                                  >  1:790673/1‑140 (MQ=255)

                                              attattCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGTTTTGTATTTCTTTTGCGTTTTTAAAACAAGAGGCAAGCCAg                                                                                               >  2:2704260/1‑140 (MQ=255)

                                              agtactCAGAGATGCTGTTTTTGTATTTTTGTTCTAAGACGAAGTCGATCATGCCGCCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAg                                                                                               <  2:2208850/135‑1 (MQ=255)

                                                 attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                            <  1:1936513/140‑1 (MQ=255)

                                                 attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                            <  1:1847411/140‑1 (MQ=255)

                                                   tCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCGTTTCCGTTTTTAAAAAAAGAGGCAAGCCCGATaaa                                                                                          >  2:483268/1‑140 (MQ=255)

                                                       ataTGCTGTTTTTGTATTTTTGTTATAATAAGAAGTAGATAATTAATAATATACTTTAGTTTTATTTTTTTTTTTTTAAGTTTTGGGATATTTGAATGGCTTTTCAGTTTTTTAATAAAGATGCAAGCCAGATaaaacag                                                                                      >  2:139448/3‑136 (MQ=255)

                                                       agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGGGATTTTAATTGCTTTTGCGTTTTTAAAAAAAGAGGCAAACCACAAAAAaccc                                                                                      >  1:1311090/1‑139 (MQ=255)

                                                       agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGGTTTGCGTTTTTAAAAAAAGAGGCAAGCCAGATAAAaccc                                                                                      >  1:2406883/1‑139 (MQ=255)

                                                       agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCg                                                                                      >  2:507391/1‑140 (MQ=255)

                                                       agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAATCCAGATAAACCCg                                                                                      >  2:1846891/1‑140 (MQ=255)

                                                       agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCg                                                                                      >  2:3643487/1‑140 (MQ=255)

                                                       agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGAATTTTTTTTGAATTTTTGGGATATTTGAATTGCTTTTCAGTTTTTTAAAAAAGAGGCAAACCACATAAAACCg                                                                                      >  2:1989148/1‑140 (MQ=255)

                                                       agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTGTTTGTAGGTTTTGGAGATTTTGATGGCGTTTGCGTTTTTTAAACAACAGGGCAGCCACCAAAACCCg                                                                                      >  1:1148512/1‑140 (MQ=255)

                                                         aTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGac                                                                                    >  2:2004985/1‑140 (MQ=255)

                                                            cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTTAATTGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAAACCGacaaa                                                                                 >  2:227650/1‑139 (MQ=255)

                                                            cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGTCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                                                 >  2:747092/1‑140 (MQ=255)

                                                            cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                                                 >  2:1859188/1‑140 (MQ=255)

                                                            cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTAAACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTATAACAAGAGGCCAGCCAGATAAACCCGacacc                                                                                 >  2:2833714/1‑138 (MQ=255)

                                                              gTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGAATTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAACAGGCAACCCACATAAACCCGacacaaa                                                                               >  1:92987/1‑136 (MQ=255)

                                                                ttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaacaaca                                                                             <  1:985714/140‑1 (MQ=255)

                                                                         tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTTAAAAAATAGGCAATCCAGATAAAACCCACAACAACAAAAaataat                                                                    >  2:2369589/1‑140 (MQ=255)

                                                                         tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAATAGGCAAGCCAGATAAACCCGACAACAACAAAAaataaa                                                                    >  2:2388181/1‑139 (MQ=255)

                                                                         tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                    >  2:870972/1‑140 (MQ=255)

                                                                         tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                  >  2:85627/1‑110 (MQ=255)

                                                                         tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                    >  2:2741292/1‑140 (MQ=255)

                                                                             ttCTAATCCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAACACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  1:1978963/140‑1 (MQ=255)

                                                                             ttCTAATACTAAGTCGATCATTCCGACTATACGTGCGTTTTCGGATTTTTGTTTGAAGTTGTGGGAGATTTGAAGGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  1:3539490/140‑1 (MQ=255)

                                                                             ttCTAATACGAAGTCGATCATTCCGACTATCCGGGCGTTTTCGGATTTTTGTATGAAGTTTTGCGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  1:3129203/140‑1 (MQ=255)

                                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  2:156488/140‑1 (MQ=255)

                                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  1:886611/140‑1 (MQ=255)

                                                                              tCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGCTTTTTTTTTGTAGTTTTTGGTGATTTGTATGGGGTTTCCGTTTTTTAAACAAGGGGCAACGCAGCTAAACCCCCCCACCACCAAAAATAATCtgag                                                               >  1:1057597/1‑137 (MQ=255)

                                                                              tCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGTAGTTTTGGGAGATTTTAATGGCTTTTGCGTTTTTAAAAAAATAGGCAAGCCACATAAACCCGACAACCACAAAAAATAATCTAGc                                                               >  1:2692725/1‑140 (MQ=255)

                                                                               cTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCa                                                              <  1:2125955/140‑1 (MQ=255)

                                                                               cTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCa                                                              <  1:1464024/140‑1 (MQ=255)

                                                                                   tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTCTTGGAAGATTCCGGAGAATTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                          <  2:2148274/140‑1 (MQ=255)

                                                                                   cccgctctCGATCAGTCCGACTATACGGGCGTTTTCGGATTTTTGTATGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                          <  2:3192233/133‑1 (MQ=255)

                                                                                     cGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTaaa                                                        <  2:3607286/140‑1 (MQ=255)

                                                                                       aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCCGATAAACCCGACAACAACAAAAAATAATCGAGCAAGTAAAcg                                                      >  2:281087/1‑139 (MQ=255)

                                                                                       aaGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTGTTTGAAGTTTTGGGCGCTTTTAATGGCTTTTCCGTTTTTTAAGCAAGAGGCCCGCCAGATAAACCCGCCCACAACCAAAAAAAATCTAGCAAGTaacca                                                      >  1:125883/1‑137 (MQ=255)

                                                                                        aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                     <  2:3186242/140‑1 (MQ=255)

                                                                                        aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                     <  2:1186235/140‑1 (MQ=255)

                                                                                         gTCGATCATTCCGACTATTCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                    <  2:3789736/140‑1 (MQ=255)

                                                                                         gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                    <  1:3312175/140‑1 (MQ=255)

                                                                                         gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                    <  2:897109/140‑1 (MQ=255)

                                                                                         gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                    <  2:2549724/140‑1 (MQ=255)

                                                                                            gATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATAGGCAAGCCAGATAAAACCGACAACAAAAAAAAATAATCTAGCAAGTAAACTCcatg                                                 >  2:3320691/1‑140 (MQ=255)

                                                                                             aTCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTTTAGTTTTTGGAGTTTTGTATTGCTTTTGCGTTTTTTAAACAAGAGGCAATCCCGATAAACCCGCCAACAACAAAAAAAAAACTAGCAAGTAAAATCcatgc                                                >  1:1604470/1‑140 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  2:544103/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:3210768/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:1030922/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  2:782478/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:3051087/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGATTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  2:3291939/140‑1 (MQ=255)

                                                                                              tCAGTCCGACTATCCGGTCGTTGTCGGATTTTTGTTTGCAGTTTTGTGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:2436627/140‑1 (MQ=255)

                                                                                                           aCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGATATTTGAATGGCTTTTACGTTTTTAAAAAAAGATGCAAGCCAGATAAAACCGACAACAACAAAAAATAATATAGCAAGTAAACTCCATGCATGGGGTAattcat                                  >  2:1157915/1‑140 (MQ=255)

                                                                                                                    ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                         <  1:1158774/140‑1 (MQ=255)

                                                                                                                               gtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt              <  1:1740756/140‑1 (MQ=255)

                                                                                                                                 ttgCAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag            <  1:3827308/140‑1 (MQ=255)

                                                                                                                                 ttgAAGTTTTGGGATATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag            <  2:3265676/140‑1 (MQ=255)

                                                                                                                                 ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag            <  2:2945154/140‑1 (MQ=255)

                                                                                                                                 ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag            <  1:2498575/140‑1 (MQ=255)

                                                                                                                                           gggAGATTTGAATGGCATTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACTACAAAAAATAATCTAGCAAGTAAACTCAATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTCCTAAt  <  2:1734315/140‑1 (MQ=255)

GTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAT  >  NZ_CP034943/240059‑240337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`