BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,843,812	C→A	9.9%	E149* (GAG→TAG)	EO946_RS19775 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,843,812	0	C	A	9.9%	695.2 / 15.9	282	E149* (GAG→TAG)	EO946_RS19775	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (169/75); new base A (3/35); total (172/110)
Fisher's exact test for biased strand distribution p-value = 2.97e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

ATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAAATTGAATTGATTCAAGG  >  NZ_CP034943/3843676‑3843951

aTGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCaaa                                                                                                                                          >  1:3676872/1‑140 (MQ=255)

aTGCTGTTCATGATTTTGACGATTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCaaa                                                                                                                                          >  2:814682/1‑140 (MQ=255)

 tGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTTATCATGCTTTCTGTGACCTCaaaa                                                                                                                                         >  2:3415404/1‑140 (MQ=255)

 tGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCaaaa                                                                                                                                         >  1:3059389/1‑140 (MQ=255)

 tGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCaaaa                                                                                                                                         >  1:2547507/1‑140 (MQ=255)

 tGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCaaaa                                                                                                                                         >  2:1136331/1‑140 (MQ=255)

   cTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCaaaaaa                                                                                                                                       >  1:1399728/1‑140 (MQ=255)

     gTTAATGATTTTGACGCTTTCAGCGCTTTTTTGCGTATATTTTTTTTTCTTTTTATAAAGCTGCTTTCTTGTCTTCTTCGCGTTTTTGGGTTTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTAAAAAAAAg                                                                                                                                     <  2:776513/140‑1 (MQ=255)

      ttCTTGATTTTGACGCTTTCCGCGCTGTATTGGTTATTTTGTTTTTTCCTTTGATCAAGCTGGTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAAGTTGGCTTATGAGTTTGTTCATGCTTTCTGTGACCTCAAAAAAAGc                                                                                                                                    <  2:2734968/140‑1 (MQ=255)

      ttCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGc                                                                                                                                    >  1:2775751/1‑140 (MQ=255)

      ttCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGc                                                                                                                                    >  1:3252894/1‑140 (MQ=255)

      ttCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGc                                                                                                                                    >  1:2082314/1‑140 (MQ=255)

      ttCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGc                                                                                                                                    >  1:1877886/1‑140 (MQ=255)

      ttCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGc                                                                                                                                    >  1:13548/1‑140 (MQ=255)

        cATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCgg                                                                                                                                  >  2:3197835/1‑140 (MQ=255)

        attGATTTTGACGCTTTCAGCGCTTTTTTGCGTATTTTGTTTTTTCAGTTGATCAAGCTGCTTTGTTGTCTTCTTGGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTAAAAAAAAGCgg                                                                                                                                  <  2:2772277/138‑1 (MQ=255)

              tttGACTCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGGTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTAt                                                                                                                            >  2:1382198/1‑140 (MQ=255)

              tttGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTTATCAAGCTGCTGTCTTTTCTTCTTCGCTTTTTTGGGTGTAACTTGGCTTATGAGTTTGATCATGATTTCTGTGACCTAAAAAAAAGCGGCTCTAt                                                                                                                            <  2:1865070/140‑1 (MQ=255)

                tGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTAttt                                                                                                                          >  1:654285/1‑140 (MQ=255)

                       ttCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTTTGACCTCAAAAAAAGCGGCTCTATtttctttaa                                                                                                                   >  2:2839460/1‑138 (MQ=255)

                       ttCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCa                                                                                                                   >  2:1505188/1‑140 (MQ=255)

                               tGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGc                                                                                                           >  2:1334162/1‑140 (MQ=255)

                               tGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGc                                                                                                           >  2:2527534/1‑140 (MQ=255)

                                     gcttataTTTTTTTTTCAGTTGATCAAGCTGTTGTCTTGTCTACTTCGCTTTTTTGGGTGTAACTTGGCTGATGATTTTTATCATGCTTTCTGTCACAAAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCg                                                                                                     <  1:3415404/137‑1 (MQ=255)

                                      cGTATATTGTTTTTTCGGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGt                                                                                                    >  1:1135017/1‑140 (MQ=255)

                                      cGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGt                                                                                                    >  2:3492062/1‑140 (MQ=255)

                                        tatcTTGTTTTTTCAGTTGATCAAGCTGCTGTGTTGTCTTCGTCGCGTTTTTCGGTGTAACGTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCCAAAAATGCGGCGCTAGTGTCTTTCAGCTGTTGCATTTCGTTg                                                                                                  >  2:701532/1‑140 (MQ=255)

                                        tataTTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTg                                                                                                  >  2:3688032/1‑140 (MQ=255)

                                        tataTTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTg                                                                                                  >  2:2022513/1‑140 (MQ=255)

                                        tataTTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTg                                                                                                  >  2:146733/1‑140 (MQ=255)

                                           atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTTCATTTCGTTGCgg                                                                                               >  2:2893635/1‑140 (MQ=255)

                                           atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                                                               >  1:280233/1‑140 (MQ=255)

                                           atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                                                               >  1:2861587/1‑140 (MQ=255)

                                           atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                                                               >  1:1475445/1‑140 (MQ=255)

                                           atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                                                               >  1:78539/1‑140 (MQ=255)

                                           atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                                                               >  2:1120965/1‑140 (MQ=255)

                                           atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                                                               >  1:2104756/1‑140 (MQ=255)

                                           atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                                                               >  1:1042931/1‑140 (MQ=255)

                                           atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                                                               >  1:2449371/1‑140 (MQ=255)

                                           atTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTAATTTCTTTCAGCTGTTGCATTTCGTTGCgg                                                                                               >  2:352798/1‑140 (MQ=255)

                                           atTGTTTTTTCAGTTGATCAAGCTGCGGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCAGTTCGTTGCgg                                                                                               >  1:3718276/1‑140 (MQ=255)

                                             ttttttttCAGTTGATCACGCTGCTGTCTTGTCTTCTGCGCTTTTTTCGGTTTACGTTGGCTGATGTTTTTTAACATGTTTTATGTGACCAAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCt                                                                                             <  1:3197835/138‑1 (MQ=255)

                                             tGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCt                                                                                             >  1:478827/1‑140 (MQ=255)

                                             tGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCt                                                                                             >  1:2252579/1‑140 (MQ=255)

                                               ttttttCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCa                                                                                           >  2:3064885/1‑140 (MQ=255)

                                                tttttCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTC‑AAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCAtt                                                                                         >  1:907420/1‑140 (MQ=255)

                                                    tCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCt                                                                                      >  2:2158272/1‑140 (MQ=255)

                                                             cAAGCTGGTGTCTTGTCTTCTTCGCGTTTTGGGTTGTAAGTTGGCTGTTGTGTTTGATCTTGCTTTCTGTGACATCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACgg                                                                             <  1:2741619/140‑1 (MQ=255)

                                                             cAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTA‑TTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACggg                                                                            >  1:3099243/1‑140 (MQ=255)

                                                              aaGCTGCTGTGTTGTCTAGTTCGCGTTTTTCGGTGTAAATTGGCTGTTGAGTTTTATAATGATTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACggg                                                                            <  2:2861773/140‑1 (MQ=255)

                                                                  tgctgTCTTGTCTTCTGCGTTTTTTTCGGTGTAAGTTGGCTGATGTTTTTTATATTGGTTTCTGTGACCAAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGtttt                                                                        <  2:1693354/140‑1 (MQ=255)

                                                                   gctgTCTTTTCTTCTTCGCTTTTTTCGGTGTACGTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGttttt                                                                       <  1:732731/140‑1 (MQ=255)

                                                                   gctgTCTTGTGTTCTTGGCTTTTTTCGGTGTAACTTGGCGTATGTTTTTGATCATGGTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGttttt                                                                       <  2:563933/140‑1 (MQ=255)

                                                                     tgtcttgtctTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTg                                                                     >  1:2311941/1‑140 (MQ=255)

                                                                     tgtcttgtctTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTGTTTg                                                                     >  2:2516984/1‑140 (MQ=255)

                                                                         ttgtcttCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGt                                                                 >  1:1238093/1‑140 (MQ=255)

                                                                          tgtcttCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTa                                                                >  1:1491688/1‑140 (MQ=255)

                                                                           tttttcttcGCTTTTTTCGGTGTAATTTGGCTGTTGATTTTTATCATGCTTTCTGTCACCTAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTaa                                                               <  1:20460/137‑1 (MQ=255)

                                                                            tcttctTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTaaa                                                              >  1:1174341/1‑140 (MQ=255)

                                                                              ttcttcGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGc                                                            >  2:2670893/1‑140 (MQ=255)

                                                                              ttcttcGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGc                                                            >  2:2490219/1‑140 (MQ=255)

                                                                              ttcttcGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGc                                                            >  1:3067973/1‑140 (MQ=255)

                                                                                 ttcGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAAc                                                         >  1:7525/1‑140 (MQ=255)

                                                                                 ttcGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAAc                                                         >  2:3727282/1‑140 (MQ=255)

                                                                                 ttcGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAAc                                                         >  2:110114/1‑140 (MQ=255)

                                                                                 ttcGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAAc                                                         >  1:2934951/1‑140 (MQ=255)

                                                                                   cgcgTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCAttt                                                                                                       >  1:2626842/1‑92 (MQ=255)

                                                                                   cgcgTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCAttt                                                                                                       <  2:2626842/92‑1 (MQ=255)

                                                                                   cgcgTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTg                                                       >  2:2997798/1‑140 (MQ=255)

                                                                                      gTTTTTGGGTGTAAGTTGGGTGTTTAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCt                                                    <  1:1683412/140‑1 (MQ=255)

                                                                                                    cTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCgtt                                      >  2:2316401/1‑140 (MQ=255)

                                                                                                       ggCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTGTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTg                                   >  1:451425/1‑140 (MQ=255)

                                                                                                         gttatgaGTTTGATAATGCTTTCTGTCACATCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAg                                 <  1:3779624/137‑1 (MQ=255)

                                                                                                         cttttcaGTTTCATCTGGATTACTGTCACCTCAAAACAAGCGGCACTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTACTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAg                                 <  1:3312715/134‑1 (MQ=255)

                                                                                                         cttatgaGTTTGATCATGCTTTCTGTTACCTCAAAAAAAGCGGCTCTAGTTTCTTTCAGCTGGTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAg                                 <  2:1941814/137‑1 (MQ=255)

                                                                                                         cggatgatTTGGATCCGGATTTCTGTGCACTAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCATCGCTGTTTTGCTTTTTGAg                                 <  1:3158976/138‑1 (MQ=255)

                                                                                                         cTGATGTTTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAg                                 <  2:3589706/140‑1 (MQ=255)

                                                                                                         cTGATGAGTTTGAGCATGCTTTCTGTGCCCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAg                                 <  2:413575/140‑1 (MQ=255)

                                                                                                          tgatgcGTTTGATCATGCTTTGTGTCACCAAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGc                                <  1:3492062/140‑1 (MQ=255)

                                                                                                          tgacgaGTTGGATCATGCTTACAGGGACCACAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGc                                <  1:2951054/140‑1 (MQ=255)

                                                                                                           gatgaGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTGTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCt                               >  1:1514682/1‑140 (MQ=255)

                                                                                                            atgaGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTg                              >  1:1747691/1‑140 (MQ=255)

                                                                                                                   tGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTttatta                       >  2:2094781/1‑140 (MQ=255)

                                                                                                                     aTCATTCTTTTTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATc                     <  2:2038341/140‑1 (MQ=255)

                                                                                                                        aTGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCaaa                  <  2:441524/140‑1 (MQ=255)

                                                                                                                         tGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAaat                 <  2:3453744/140‑1 (MQ=255)

                                                                                                                           cTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAaattg               >  1:2008584/1‑140 (MQ=255)

                                                                                                                             tttttttaCCTAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAaattgaa             <  1:1917005/133‑1 (MQ=255)

                                                                                                                             ttCTTTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTGATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAaattgaa             <  2:1192556/140‑1 (MQ=255)

                                                                                                                             ttCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAaattgaa             <  2:626967/140‑1 (MQ=255)

                                                                                                                             ttCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAaattgaa             <  1:2719610/140‑1 (MQ=255)

                                                                                                                             ttCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAaattgaa             <  2:1045415/140‑1 (MQ=255)

                                                                                                                             ttCTGTGACCTAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAaattgaa             <  1:439006/140‑1 (MQ=255)

                                                                                                                             ttCTGTGACAACAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAaattgaa             <  1:969261/140‑1 (MQ=255)

                                                                                                                              tCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAaattgaat            <  1:2490219/140‑1 (MQ=255)

                                                                                                                                tgtgACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGGTGTTTATTATCAaattgatttg          >  1:2197951/1‑140 (MQ=255)

                                                                                                                                   gACCTAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAAATTGAATTGAtt       <  2:2965413/140‑1 (MQ=255)

                                                                                                                                     ccTAAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAAATTGAATTGATTCa     <  2:159807/140‑1 (MQ=255)

                                                                                                                                       tCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAAATTGAATTGATTCAAg   >  1:625777/1‑140 (MQ=255)

                                                                                                                                        cAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTGATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAAATTGAATTGATTCAAgg  <  2:2372842/140‑1 (MQ=255)

ATGCTGTTCATGATTTTGACGCTTTCAGCGCTGTATTGCGTATATTGTTTTTTCAGTTGATCAAGCTGCTGTCTTGTCTTCTTCGCGTTTTTCGGTGTAACTTGGCTGATGAGTTTGATCATGCTTTCTGTGACCTCAAAAAAAGCGGCTCTATTTTCTTTCAGCTGTTGCATTTCGTTGCGGCTCATTTCTTGATTACGGGTTTTTTGGAGTAAAGCAACTGCCTCGCTGTTTTGCTTTTTGAGCTGTTTATTATCAAATTGAATTGATTCAAGG  >  NZ_CP034943/3843676‑3843951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`