BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,237	G→T	6.9%	I46I (ATC→ATA)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,237	0	G	T	6.9%	781.3 / 11.0	321	I46I (ATC→ATA)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (212/71); new base T (7/29); total (219/102)
Fisher's exact test for biased strand distribution p-value = 1.24e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

TGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATA  >  NZ_CP034943/3851101‑3851375

tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                         <  1:1091437/140‑1 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTTCCTGTATCATGATTCg                                                                                                                                       >  1:3205867/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTCTCATGATTCg                                                                                                                                       >  2:76779/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTct                                                                                                                                       >  1:3643229/1‑139 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                       >  1:963587/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                       >  1:1474060/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                       >  1:1571243/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                       >  1:2872025/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGTATTACCTGTATCATGATTCg                                                                                                                                       >  1:2826805/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTAATCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAGCTGTATAATGATTCg                                                                                                                                       >  1:451219/1‑140 (MQ=255)

    ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                                     <  2:852307/140‑1 (MQ=255)

     cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                    <  2:2746006/140‑1 (MQ=255)

     cAATCTGCTCTCTGTAATATGGATTCGAACTGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                    <  1:2058896/140‑1 (MQ=255)

       aTCTGCTCTCTGTAATATTGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGTTCTTCTATAAATGAACCAGTCGTTATTACATGTATAATGATTAGATctt                                                                                                                                  >  2:2973363/1‑138 (MQ=255)

       aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                                  >  2:1560476/1‑140 (MQ=255)

       aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCAGAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                                  >  1:2623308/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                             >  2:691713/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                             >  2:371572/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                             >  2:1752713/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                             >  1:2200118/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACGTGTATCATGATTCGATCGTTTGCa                                                                                                                             >  2:2022296/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATAAAGATTAGATCGTATgga                                                                                                                             >  1:2054009/1‑138 (MQ=255)

              ctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAg                                                                                                                           >  1:3787870/1‑140 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAATCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTTTTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                        >  1:3583289/1‑140 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                        <  1:3384630/140‑1 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGccc                                                                                                                        >  1:1758964/1‑140 (MQ=255)

                  gTAATATGGATTGGAAATGGAATTCCCTTTTGGCCCGCTTCGTCACCTTGTAAGCATGACCTTCAAAATACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCt                                                                                                                       >  2:3078029/1‑140 (MQ=255)

                        tGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGGAAGCCCTTCCCaa                                                                                                                 >  1:588659/1‑140 (MQ=255)

                         ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                >  2:369234/1‑140 (MQ=255)

                         gcagtcgAAATGGAAGTATCTTTTCGATAGTTTTATCAAGTTTTAAGCATAACCATCAAATTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTCTGCAAGCCCTTCCCaaa                                                                                                                <  2:2655966/134‑1 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTCCTCGGTCAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCGCTTCCCaaaca                                                                                                              >  2:3864705/1‑138 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                         >  2:1461786/1‑113 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCaaaaa                                                                                                              >  1:2653638/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                              >  2:342090/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                              >  2:102044/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                              >  2:1121631/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                              >  2:1828754/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                              >  2:3141542/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCCaaaaa                                                                                                              >  2:306413/1‑140 (MQ=255)

                            ttGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGCTCGGATGCACGCCCGTCCCAAGAAc                                                                                                             >  1:513535/1‑140 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                            >  2:3812793/1‑140 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                            >  1:2370755/1‑140 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                            >  2:2149812/1‑140 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                            >  1:837267/1‑140 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                            >  1:30679/1‑140 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCAAGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                            >  1:2440886/1‑140 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTAGCTGTATCATGATTCGATCGTATGCAAGCCCTTGCCaaaaaag                                                                                                            >  1:2123785/1‑138 (MQ=255)

                                aaaTGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                         <  2:1988410/108‑1 (MQ=255)

                                  aTGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATaaa                                                                                                       >  2:1377087/1‑140 (MQ=255)

                                     gAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACg                                                                                                    >  2:2823195/1‑140 (MQ=255)

                                                       ttttATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTACATAGTATGCAAGCCCTTCCCAAAAACGATAAAACGACACTGCTTCTTaaatgt                                                                                  >  1:1676660/1‑137 (MQ=255)

                                                       ttttATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGtt                                                                                  >  1:2994845/1‑140 (MQ=255)

                                                       ttttATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTATGCTTCTTCAAGtt                                                                                  >  1:3018963/1‑140 (MQ=255)

                                                           aTCACATTGTAAGAATAACATTAAACGTAATCGTACAGGTATTATAAAAATGAACCTTTCTTTATTACCTGTTACTTGATTCGATCGTATGAAACCCCTACCAAAAAACGTTAACACGACTCTGCTTCTTCAAGTTCGCa                                                                              <  2:3238384/140‑1 (MQ=255)

                                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                               >  2:3540517/1‑140 (MQ=255)

                                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                               >  2:3602246/1‑140 (MQ=255)

                                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                               >  2:50534/1‑140 (MQ=255)

                                                                           aaacTTAAAAGTACTCGGACTGTTCTTATATAAATGAACAAGTCGTTATTACCTGTATCATGATTCGATCGTATGCACGCCCTTCCCAAAAACCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtga                                                              <  2:2872025/137‑1 (MQ=255)

                                                                           aaCCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGTTAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAGAACAACTtga                                                              >  2:541550/1‑140 (MQ=255)

                                                                           aaCCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtga                                                              >  2:2429104/1‑140 (MQ=255)

                                                                           aaCCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAACTTCGCAAGACATAACAACTtga                                                              >  2:2760343/1‑140 (MQ=255)

                                                                           aaCCTACAATGTACGCGGCCAGGTCTTCTATAAATCACCCTGTGTTTTTTACCTTTATCTTGATTCTACGGTCGCCACCCCCTCCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtga                                                              <  2:1474060/140‑1 (MQ=255)

                                                                             ccTTCACAGTACAGGCACTGGTCTTCTATAATTTAACCAGTCTTTTTTACCTTTATCATTATACGAGCGTATGAAACCCCTTCAAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                            <  2:1306552/140‑1 (MQ=255)

                                                                             ccTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                            >  1:1796253/1‑140 (MQ=255)

                                                                             ccTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                            >  1:13778/1‑140 (MQ=255)

                                                                             ccTTAAAACTACTCGCACTGTTCTTCAAAAAATCAACCATTCGTTATTACCTTTATAATGTTTCGTTCTTCTGCAACCCCTCCCAAAAAACGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                            <  1:3497238/140‑1 (MQ=255)

                                                                             ccTACAAATTACTCGGACAGGTATTCTATAAATCAACCAGTGGTTATTACCTTTATCATGATTCGATCGTAGGCAACCCCTTCCAAAAACCGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                            <  2:2200118/140‑1 (MQ=255)

                                                                                  aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTTTATCAAGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCAGCTTATTCAACTTCGCAAGACATAACAACTTGATTAGGCt                                                       >  1:1936904/1‑140 (MQ=255)

                                                                                        cTCGGACTGTTTTTCTTTAAATCAACCAGTCGTTATCACCTGTTTCATTATGCGTTCGTAGGCACGCCGTCCCCAAAAACGTTAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                 <  2:2504934/140‑1 (MQ=255)

                                                                                        cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                 >  2:415413/1‑140 (MQ=255)

                                                                                        cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAACACATAACAACTTTATGAGGCTTAagag                                                 >  1:1853845/1‑140 (MQ=255)

                                                                                        cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTAAAGTTCGCAACACATAACAACTTGATGAGGCGTAacag                                                 >  2:788309/1‑140 (MQ=255)

                                                                                        cTCGGACAGGTCTGCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACGTGAGTAGGCGTAagat                                                 >  2:840442/1‑139 (MQ=255)

                                                                                          cGGACATGTCTAATATAAATCAACCAGTCTTTTTTACCTTTATCATGATTCTATCGTATCAAAGCCCTACACAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCg                                               <  2:300392/140‑1 (MQ=255)

                                                                                                ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCGAGACATAACAACTTGCTGAGGCTTAATAGCGATCCTg                                         >  1:723657/1‑140 (MQ=255)

                                                                                                ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTGTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGATCGATCATg                                         >  2:2456388/1‑140 (MQ=255)

                                                                                                ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAATTTCGCAAGACATAACAACTTTATGAGGCTTAATAGctgtctct                                         >  2:2577564/1‑133 (MQ=255)

                                                                                                ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                         >  1:2731772/1‑140 (MQ=255)

                                                                                                ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGAGATCATg                                         >  2:236655/1‑140 (MQ=255)

                                                                                                ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGAGGCTTAAGAGCGATCATg                                         >  2:3457444/1‑140 (MQ=255)

                                                                                                ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                         >  1:1135455/1‑140 (MQ=255)

                                                                                                 gTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGt                                        >  1:3454977/1‑140 (MQ=255)

                                                                                                           aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                              >  2:1191401/1‑140 (MQ=255)

                                                                                                            aTGAACCAGTCGTTATTACCTGTATCCTGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                             >  2:611010/1‑140 (MQ=255)

                                                                                                            aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATAAAGTCTAAAGTTCCg                             >  2:1269947/1‑140 (MQ=255)

                                                                                                               accctgTCTTTATTACCTTTATCATTTTTCTATCGTATGCACCCCCTACCCAAAACCGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                          <  1:3812793/135‑1 (MQ=255)

                                                                                                               acccatccGTTATTCCCTGTATCAGGATTCTATCGTATCCACCCCGTTCCCAAAAACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                          <  2:1672374/133‑1 (MQ=255)

                                                                                                               acccAGTCTTATTTACATGAATAATGTTTCTATCTTATCCAAGCCCTGCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                          <  2:3759659/138‑1 (MQ=255)

                                                                                                               aaCCTGTGGTTTTTACTTTTATCATTATTCGATGGTATGCAAGCCCTCCCCAAAAACCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                          <  1:3651800/140‑1 (MQ=255)

                                                                                                               aaCCAGTCGTTATTACCTGTATCTTGATTCGATCTTATGCACGCCCTTCCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                          <  2:3269500/140‑1 (MQ=255)

                                                                                                                aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCGGCTTCTTCAAGTTCGCAAGACCTAACAACTTGATGAGGGTTAAGAGCGATCATGTCTAAATTGCCGATAc                         >  2:318971/1‑140 (MQ=255)

                                                                                                                aCCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACACTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc                         >  1:89870/1‑140 (MQ=255)

                                                                                                                 ccAGTCGTTTTTACTTGCATCTTGATTCTATCTTTTGCACGCCCTCCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                        <  2:3290206/140‑1 (MQ=255)

                                                                                                                     tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAATAGCGATCATTTCTAAATTGCCGATAATGCtt                    >  1:2562618/1‑140 (MQ=255)

                                                                                                                         ttttaCCTGTATCATTTTTCGATCCTCTGCACGCCCGTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAg                <  2:474976/138‑1 (MQ=255)

                                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt       >  1:1000525/1‑140 (MQ=255)

                                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt       >  2:1729594/1‑140 (MQ=255)

                                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt       >  2:165139/1‑140 (MQ=255)

                                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt       >  1:689724/1‑140 (MQ=255)

                                                                                                                                       tGATTCTATCGTATGCCCGCCCTCCCAAAAAACGAGAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACACCTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa  <  1:3708423/140‑1 (MQ=255)

TGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATA  >  NZ_CP034943/3851101‑3851375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`