BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,906,067	A→C	12.0%	I42S (ATT→AGT)	EO946_RS20100 ←	DUF3255 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,906,067	0	A	C	12.0%	621.3 / 10.0	314	I42S (ATT→AGT)	EO946_RS20100	DUF3255 family protein
Reads supporting (aligned to +/- strand): ref base A (154/103); new base C (51/2); total (209/105)
Fisher's exact test for biased strand distribution p-value = 1.84e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

AGTACTTTGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTTCCTCCTCTTT  >  NZ_CP034943/3905928‑3906206

aGTACTTTGGGAAACCACCTTGTATGCCATAAAAGGCATTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGTGTaa                                                                                                                                             >  1:3471111/1‑140 (MQ=255)

 gTACTTTGGGCAACCACCGGGTATGCCATAAAAGGCAGTGGCACCTCTCCTCTTCCTCCAAGCACTTCCGTCTCCACCACCGCGTCTTTGGTACAGGTGACTGTTGCTGATTTCACGCCGGCCATGGGTCCCGGGGTAAt                                                                                                                                            <  2:2579621/140‑1 (MQ=255)

     tttGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTccc                                                                                                                                        >  2:854199/1‑140 (MQ=255)

     tttGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTccc                                                                                                                                        <  2:2071228/140‑1 (MQ=255)

     tttGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTccc                                                                                                                                        >  2:3564586/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTc                                                                                                                                      >  1:2967921/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTc                                                                                                                                      >  2:453728/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  1:1982869/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  2:3327280/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  1:2578025/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  1:2158764/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  1:1678391/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCGCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  2:3831721/1‑140 (MQ=255)

            aaCCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTACATGGTTCCCGTGGTAATTCCCTCATccc                                                                                                                                 >  2:3767587/1‑140 (MQ=255)

                    tGTATGCCATAAAAGGCAGTTGCACCTCTCCTCGTCCTTCAATCACGTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGTGTCCCGGGGTAATTCCCTCATCCCACGTTCCg                                                                                                                         <  2:2537202/140‑1 (MQ=255)

                     gTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGa                                                                                                                        <  2:1934130/140‑1 (MQ=255)

                     gTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGa                                                                                                                        >  2:1230128/1‑140 (MQ=255)

                        tGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCCGTCCCTCATCCCACGTTCCGAAAt                                                                                                                     >  1:1237466/1‑140 (MQ=255)

                         gCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATa                                                                                                                    >  1:183323/1‑140 (MQ=255)

                         gCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATa                                                                                                                    >  1:2018000/1‑140 (MQ=255)

                           cATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCATTCCCTCATCCCACGTTCCGAAATaaa                                                                                                                  >  1:3211405/1‑140 (MQ=255)

                            aTAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATaaaa                                                                                                                 >  2:582598/1‑140 (MQ=255)

                             tAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATacaaa                                                                                                                >  2:3031941/1‑140 (MQ=255)

                                     aGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTc                                                                                                        >  1:1118929/1‑140 (MQ=255)

                                     aGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCAACCACCTCGTCTTTGGTAAAGGTGACTTTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAATGCATTTc                                                                                                        >  2:289946/1‑140 (MQ=255)

                                               ctcctctTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTttgt                                                                                              >  1:292474/1‑140 (MQ=255)

                                               ctcctctTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTttgt                                                                                              >  2:3307958/1‑140 (MQ=255)

                                               ctcctctTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGGGACTGTTGCTGATTTCACGCCGTCCCTGGGTCCCGGGGTCCTTGCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTttgt                                                                                              >  1:1590494/1‑140 (MQ=255)

                                                     ttccgtcAATCACGTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAACAAGGCATTTCCTCTTTTTGTTGTTGa                                                                                        <  2:1510599/140‑1 (MQ=255)

                                                       ccttcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACa                                                                                      >  1:1171236/1‑140 (MQ=255)

                                                       ccttcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACa                                                                                      <  1:3263569/140‑1 (MQ=255)

                                                          tcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCCTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGcc                                                                                   >  1:2880300/1‑140 (MQ=255)

                                                          tcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGcc                                                                                   >  1:460507/1‑140 (MQ=255)

                                                          tcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGcc                                                                                   >  1:3201404/1‑140 (MQ=255)

                                                          tcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGcc                                                                                   >  1:2427002/1‑140 (MQ=255)

                                                          tcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGcc                                                                                   <  2:3447392/140‑1 (MQ=255)

                                                          tcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTGTTTGTTGTTGACAGcc                                                                                   >  2:1865961/1‑140 (MQ=255)

                                                          tcAATCACGTCCGTCTCCACCACCGCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCAGCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTGTTGTTGTTGACAGcc                                                                                   <  2:3372619/140‑1 (MQ=255)

                                                            aaTCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCg                                                                                 >  1:2819691/1‑140 (MQ=255)

                                                               cACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              <  1:3446608/140‑1 (MQ=255)

                                                               cACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              <  1:3741887/140‑1 (MQ=255)

                                                               cACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              <  1:1533252/140‑1 (MQ=255)

                                                               cACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              <  1:3116772/140‑1 (MQ=255)

                                                               cACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              <  1:2514309/140‑1 (MQ=255)

                                                               cACGTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              <  2:1313070/140‑1 (MQ=255)

                                                                 cTTCCGTCGCCACCACCTCGTCTTTGCTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAAc                                                                            <  2:3686563/140‑1 (MQ=255)

                                                                 cTTCCGGCTCCACCCGCTCGTCTTTGGTACAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCGCGGGGTAATTCCCTCCTCCCACGTTCCGAAATACATTGGGATTTCCTCTTTTTGTTGTTGACAGCCCGAGAAc                                                                            <  2:3220084/140‑1 (MQ=255)

                                                                    ccGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTg                                                                         <  1:2372216/140‑1 (MQ=255)

                                                                    ccGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTg                                                                         <  2:142015/140‑1 (MQ=255)

                                                                    cagTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTg                                                                         <  2:865618/138‑1 (MQ=255)

                                                                      gTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAACAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGAt                                                                       <  2:1675068/140‑1 (MQ=255)

                                                                       tctcCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATg                                                                      >  2:104077/1‑140 (MQ=255)

                                                                         gccaccaCCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGTGTAATTCCCTCATCCCACGGTCCGAAATAAAAACGCATTTCCACTTTTTGTTGTTGACAGCCCGATAACGTGATGca                                                                    <  1:247689/139‑1 (MQ=255)

                                                                            accaccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCCTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGcacac                                                                 >  1:2629653/1‑140 (MQ=255)

                                                                            accaccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGcacac                                                                 >  1:1022529/1‑140 (MQ=255)

                                                                            accaccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATGCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGcacac                                                                 >  2:1396851/1‑140 (MQ=255)

                                                                             ccaccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGcacaca                                                                <  2:3853441/140‑1 (MQ=255)

                                                                              caccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCCTGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACAcag                                                               <  1:3511394/140‑1 (MQ=255)

                                                                              caccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACAcag                                                               <  2:2111295/140‑1 (MQ=255)

                                                                              caccTCGTCTTTGGTAAAGGGGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCTGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACAcag                                                               <  2:2315277/140‑1 (MQ=255)

                                                                                   cGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATaa                                                                             <  1:3056281/121‑1 (MQ=255)

                                                                                     tCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTaa                                                        >  2:1572892/1‑140 (MQ=255)

                                                                                     tCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTaa                                                        >  1:2274352/1‑140 (MQ=255)

                                                                                     tCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGATaa                                                        >  2:2748505/1‑140 (MQ=255)

                                                                                           gTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCaa                                                  >  1:1871413/1‑140 (MQ=255)

                                                                                           gTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCaa                                                  >  1:2124962/1‑140 (MQ=255)

                                                                                                ggTGACTGTTGCTGATTTCACGCCGTCCATGGGTCACGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGAAGTTAAAAGCAAAATGc                                             >  2:1121514/1‑140 (MQ=255)

                                                                                                    aCTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCGTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCcaca                                         >  2:419430/1‑140 (MQ=255)

                                                                                                    aCTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCAGTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCcacc                                         >  2:763189/1‑139 (MQ=255)

                                                                                                      tGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACAtt                                       >  1:693072/1‑140 (MQ=255)

                                                                                                      tGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACAtt                                       >  1:3698247/1‑140 (MQ=255)

                                                                                                       gTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACAttt                                      <  1:854199/140‑1 (MQ=255)

                                                                                                       gTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACAttt                                      <  1:3342812/140‑1 (MQ=255)

                                                                                                       gTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACAttt                                      >  1:1443339/1‑140 (MQ=255)

                                                                                                          gCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGtt                                   >  2:3079738/1‑140 (MQ=255)

                                                                                                           cTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGttt                                  >  1:3391774/1‑140 (MQ=255)

                                                                                                           cTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGttt                                  >  1:2679801/1‑140 (MQ=255)

                                                                                                              aTTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTtctc                               >  1:2332046/1‑140 (MQ=255)

                                                                                                              aTTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTtctc                               <  2:2158764/140‑1 (MQ=255)

                                                                                                              aTTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTtctc                               <  1:743783/140‑1 (MQ=255)

                                                                                                                  cACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACCCAGCAGTTAAAAGCAAAATGCCCCCTTTGTTTCTCACgg                           >  2:3141818/1‑140 (MQ=255)

                                                                                                                   aCGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGt                          <  1:3082434/140‑1 (MQ=255)

                                                                                                                   aCGCCGTCCATGGCTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGt                          <  2:2838468/140‑1 (MQ=255)

                                                                                                                         tCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCAc                    >  2:3651476/1‑140 (MQ=255)

                                                                                                                          ccATGGGTCCCGGGGTAAGTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCATACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCAcc                   <  2:2431001/140‑1 (MQ=255)

                                                                                                                                 tCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCttt            >  2:2957296/1‑140 (MQ=255)

                                                                                                                                 tCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCttt            >  2:2260446/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTCGGTCCCTCATCCCACGGTCCGAAATAAAAAGGCATTGCCTCTTTGTGTTGTTGACAGCCCGATAACGTGATGCACCCAGCAGTTAAAAGCACAATGCCACCTTTGTTTCTCACGGGGTTCTCCATCCTGtcc          >  1:3820090/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTCATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:3581578/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:3856376/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGcaca                                                                  >  2:1777062/1‑84 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCa                                                   <  1:1368209/99‑1 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGc                                             <  1:935365/105‑1 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  2:1150337/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:3652184/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  2:1641498/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:517804/1‑140 (MQ=255)

                                                                                                                                      gggTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcctcc       <  2:1854680/140‑1 (MQ=255)

                                                                                                                                          aaTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcctcc       <  1:1663788/136‑1 (MQ=255)

                                                                                                                                           aTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATGTGTTTCTCAAGGTGATCACCATCCTTTCCTCCTCttt  >  1:805418/1‑140 (MQ=255)

AGTACTTTGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTTCCTCCTCTTT  >  NZ_CP034943/3905928‑3906206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`