BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,207	G→T	10.5%	Q109K (CAA→AAA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,207	0	G	T	10.5%	676.0 / 9.4	310	Q109K (CAA→AAA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (117/146); new base T (45/2); total (162/148)
Fisher's exact test for biased strand distribution p-value = 2.96e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

GTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCG  >  NZ_CP034943/240068‑240343

 tgtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGa                                                                                                                                         >  1:935298/1‑140 (MQ=255)

  gtAAGAGAGTGAGAAGGCGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                                        <  2:534394/140‑1 (MQ=255)

  gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGTATTTTTGGGAGATTTGaa                                                                                                                                        >  2:2887929/1‑140 (MQ=255)

  gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                                        <  2:3303859/140‑1 (MQ=255)

         agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAATTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTTGGAGATTTTAATGGCttt                                                                                                                                 >  2:3463177/1‑140 (MQ=255)

          gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCtttt                                                                                                                                >  2:3150114/1‑140 (MQ=255)

          gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGGTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATTGCtttt                                                                                                                                >  2:161783/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGGTTTTTGTTTGAAGTTTTGGGAGATTTTGATGGCttttg                                                                                                                               >  1:56699/1‑139 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGTTTTGAATGTCTTTTc                                                                                                                               >  2:2567187/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTc                                                                                                                               >  2:663407/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCTTTTc                                                                                                                               >  1:1589166/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                               >  1:1134782/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCtttta                                                                                                                               >  2:1189384/1‑139 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTGTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCtttta                                                                                                                               >  1:724156/1‑139 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATGGCttttg                                                                                                                               >  1:503849/1‑139 (MQ=255)

                 ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGtttt                                                                                                                         >  2:3572787/1‑140 (MQ=255)

                       tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTTAGTTTTGGGAGATTTGTATGGGTTTTTCGGTTTTaaaaa                                                                                                                   >  1:1296207/1‑139 (MQ=255)

                       tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAc                                                                                                                   >  2:3669098/1‑140 (MQ=255)

                        ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                  <  2:574602/140‑1 (MQ=255)

                         tctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAACaa                                                                                                                 >  2:199934/1‑140 (MQ=255)

                         tcggATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGCCGTTTTGGGATTTTTGTTTTAAGTTTTTGGATATTTGAATGGGTTTTACGTTTTTAaaaaaa                                                                                                                 >  1:3789736/4‑140 (MQ=255)

                          ctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTTAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACaat                                                                                                                >  2:2381613/1‑139 (MQ=255)

                          ctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCGTTTCCGTTTTTAAAACAag                                                                                                                >  2:1934825/1‑140 (MQ=255)

                             aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATGCGGGCGTTTTAGGATTTTTTTTTTAAGTTTTGGGAGTGTTTAATTGCTTTTCGGTTTTTTAAGAAAGAgg                                                                                                             >  1:2082795/1‑140 (MQ=255)

                             aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTTAGTTTTTGGAGATTTTAATTGCTTTTCCGTTTTTAAAAAAAGAgg                                                                                                             >  1:1376360/1‑140 (MQ=255)

                              tGTTTGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGc                                                                                                            <  2:239872/140‑1 (MQ=255)

                                 tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                         <  2:935693/140‑1 (MQ=255)

                                 agcacttattCAGAGATGCTCTTTCTGTATTTTTCTTCTAATCCGAAGTCGATCAGTCCGACTATACGCGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                         <  1:3303757/135‑1 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTTTTTTTAGTTTTTGGAGAATTTTATTGGTTTTACGTTTTTAAAACAAAAGGCaacc                                                                                                        >  1:2325513/1‑138 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATTGGTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                        >  1:1844051/1‑140 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGTGAGATTTTAATTGGTTTTAAGTTTTTAAAAAAAAAGGCaaac                                                                                                        >  1:3205379/1‑138 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                        >  2:436954/1‑140 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAATAGAATAGGCAAGc                                                                                                        >  2:2324750/1‑140 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGGTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAATAGGCAAGc                                                                                                        >  2:1896459/1‑140 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATGGCTTTTACGGTTTTTAAAAAATAGGCAAGc                                                                                                        >  1:2575712/1‑140 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAAAAAGAGGCAAGc                                                                                                        >  1:790673/1‑140 (MQ=255)

                                     attattCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGTTTTGTATTTCTTTTGCGTTTTTAAAACAAGAGGCAAGCCAg                                                                                                     >  2:2704260/1‑140 (MQ=255)

                                     agtactCAGAGATGCTGTTTTTGTATTTTTGTTCTAAGACGAAGTCGATCATGCCGCCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAg                                                                                                     <  2:2208850/135‑1 (MQ=255)

                                        attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                                  <  1:3341505/140‑1 (MQ=255)

                                           cAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAAc                                                                                               <  1:1699117/140‑1 (MQ=255)

                                              agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGGTTTGCGTTTTTAAAAAAAGAGGCAAGCCAGATAAAaccc                                                                                            >  1:2406883/1‑139 (MQ=255)

                                              agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCg                                                                                            >  2:507391/1‑140 (MQ=255)

                                              agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAATCCAGATAAACCCg                                                                                            >  2:1846891/1‑140 (MQ=255)

                                              agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTGTCCGATTTTTGTTTGAAATTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAAAGGCAACCCAGATAAAccag                                                                                            >  2:3104380/1‑138 (MQ=255)

                                              agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTGTTTGTAGGTTTTGGAGATTTTGATGGCGTTTGCGTTTTTTAAACAACAGGGCAGCCACCAAAACCCg                                                                                            >  1:1148512/1‑140 (MQ=255)

                                              agaTGCGGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCCTTTTTAAAACAAGAGGGAAGCCCGATAAACCCg                                                                                            >  2:1172117/1‑140 (MQ=255)

                                                aTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCCGATAAAACCGac                                                                                          >  2:2532814/1‑140 (MQ=255)

                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGTCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                                                       >  2:747092/1‑140 (MQ=255)

                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                                                       >  2:1859188/1‑140 (MQ=255)

                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                                                       >  2:227498/1‑140 (MQ=255)

                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTAAACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTATAACAAGAGGCCAGCCAGATAAACCCGacacc                                                                                       >  2:2833714/1‑138 (MQ=255)

                                                                tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTTAAAAAATAGGCAATCCAGATAAAACCCACAACAACAAAAaataat                                                                          >  2:2369589/1‑140 (MQ=255)

                                                                tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAATAGGCAAGCCAGATAAACCCGACAACAACAAAAaataaa                                                                          >  2:2388181/1‑139 (MQ=255)

                                                                tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                          >  2:870972/1‑140 (MQ=255)

                                                                tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                        >  2:85627/1‑110 (MQ=255)

                                                                tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                          >  2:2741292/1‑140 (MQ=255)

                                                                  ggTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGAGTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCt                                                                        <  2:1505202/139‑1 (MQ=255)

                                                                    ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                      <  1:3062441/140‑1 (MQ=255)

                                                                    ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                      <  1:886611/140‑1 (MQ=255)

                                                                    ttCTAAGACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                      <  1:1979660/140‑1 (MQ=255)

                                                                     tCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGCTTTTTTTTTGTAGTTTTTGGTGATTTGTATGGGGTTTCCGTTTTTTAAACAAGGGGCAACGCAGCTAAACCCCCCCACCACCAAAAATAATCtgag                                                                     >  1:1057597/1‑137 (MQ=255)

                                                                     tCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGTAGTTTTGGGAGATTTTAATGGCTTTTGCGTTTTTAAAAAAATAGGCAAGCCACATAAACCCGACAACCACAAAAAATAATCTAGc                                                                     >  1:2692725/1‑140 (MQ=255)

                                                                      cTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCa                                                                    <  1:2125955/140‑1 (MQ=255)

                                                                      cTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGATTTTAACACAAGAGGCAAGCCAGATAAACCCGACAACAACAAATAATAATCTAGCa                                                                    <  1:3734356/140‑1 (MQ=255)

                                                                          tccGAAGTCGATCAGTCCGACTATACGGGCGGTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                <  2:3282217/138‑1 (MQ=255)

                                                                          tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTCTTGGAAGATTCCGGAGAATTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                <  2:2148274/140‑1 (MQ=255)

                                                                          cccgctctCGATCAGTCCGACTATACGGGCGTTTTCGGATTTTTGTATGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                <  2:3192233/133‑1 (MQ=255)

                                                                              aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACt                                                            >  2:484210/1‑140 (MQ=255)

                                                                              aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCCGATAAACCCGACAACAACAAAAAATAATCGAGCAAGTAAAcg                                                            >  2:281087/1‑139 (MQ=255)

                                                                              aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACt                                                            <  2:3222626/140‑1 (MQ=255)

                                                                              aaGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTGTTTGAAGTTTTGGGCGCTTTTAATGGCTTTTCCGTTTTTTAAGCAAGAGGCCCGCCAGATAAACCCGCCCACAACCAAAAAAAATCTAGCAAGTaacca                                                            >  1:125883/1‑137 (MQ=255)

                                                                               aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                           <  2:3186242/140‑1 (MQ=255)

                                                                                gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                          <  2:897109/140‑1 (MQ=255)

                                                                                    aTCATTCCGACTATACGGGCGTTGTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAACAGGCAAGCCCCAAAAAACCGACAACAACAAAAAATAAAGGAGCAATTAAACAGcatgg                                                      >  1:1420473/1‑139 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  1:1030922/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  2:782478/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  1:3426272/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  2:138522/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  2:1209202/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  2:1011114/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  2:105080/140‑1 (MQ=255)

                                                                                     tCAGTCCGACTATCCGGTCGTTGTCGGATTTTTGTTTGCAGTTTTGTGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                     <  1:2436627/140‑1 (MQ=255)

                                                                                                 taCGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGGTTTTCCGTTTTTTAAACAATATGCAAGCCACATAAAACCGACAACAACAAAAAAAAATCTAACAAGTAAACACCATGCATGGGGTAattca                                         >  1:2757187/1‑140 (MQ=255)

                                                                                                 taCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGATATTTGAATGGGTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAattca                                         >  2:3628230/1‑140 (MQ=255)

                                                                                                 taCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGAAAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAAATCCATGCATGGGGTAattaa                                         >  2:110911/1‑138 (MQ=255)

                                                                                                           ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGGAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                               <  1:3591020/140‑1 (MQ=255)

                                                                                                           ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                               <  1:1158774/140‑1 (MQ=255)

                                                                                                               gATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                           <  2:3354458/140‑1 (MQ=255)

                                                                                                               gATTTGTGTTTGAATTTTTGGTAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGAAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                           <  1:2944940/140‑1 (MQ=255)

                                                                                                                        ttgCAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                  <  1:3827308/140‑1 (MQ=255)

                                                                                                                        ttgAATTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                  <  1:1934825/140‑1 (MQ=255)

                                                                                                                        ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                  <  1:2498575/140‑1 (MQ=255)

                                                                                                                        ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                  <  1:1946038/140‑1 (MQ=255)

                                                                                                                        ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                  <  1:589718/140‑1 (MQ=255)

                                                                                                                        ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                  <  2:2945154/140‑1 (MQ=255)

                                                                                                                                  gggAGATTTGAATGGCATTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACTACAAAAAATAATCTAGCAAGTAAACTCAATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTCCTAAt        <  2:1734315/140‑1 (MQ=255)

                                                                                                                                       aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc   <  2:1134855/140‑1 (MQ=255)

                                                                                                                                       aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc   <  1:3230906/140‑1 (MQ=255)

                                                                                                                                        tttGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCg  <  1:3328280/140‑1 (MQ=255)

                                                                                                                                        tttGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCg  <  2:3504941/140‑1 (MQ=255)

GTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCG  >  NZ_CP034943/240068‑240343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`