Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 240,218 C→A 5.6% G105V (GGA→GTA)  EO946_RS01360 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943240,2180CA 5.6% 744.7 / 5.9 317G105V (GGA→GTA) EO946_RS01360hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (91/185);  new base A (28/2);  total (130/187)
Fisher's exact test for biased strand distribution p-value = 7.52e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 329 total aligned reads displayed.

AGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTACTGTTT  >  NZ_CP034943/240079‑240356
                                                                                                                                           |                                                                                                                                          
agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTTAATGGCTTTTc                                                                                                                                            >  2:2383623/1‑140 (MQ=255)
agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCTTTTc                                                                                                                                            >  1:1589166/1‑140 (MQ=255)
agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                            >  1:1134782/1‑140 (MQ=255)
  agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGGTTTTGCg                                                                                                                                          >  1:3333630/1‑140 (MQ=255)
      ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAACTTTTTGGATATTTGAAAGGCTTTTACGtttt                                                                                                                                      >  2:524852/1‑140 (MQ=255)
      ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTTAGTTTTTGGAGATTTTTATTGCTTTTGCGgttt                                                                                                                                      >  1:1228843/1‑140 (MQ=255)
      ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGTATTTTTTTGAGATTTGAATTGCTTTTCCGtttt                                                                                                                                      >  2:3539490/1‑140 (MQ=255)
            tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAc                                                                                                                                >  2:3669098/1‑140 (MQ=255)
              tctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACaa                                                                                                                              >  1:897109/1‑140 (MQ=255)
              tctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCCAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTCCGTTTTTAAAACaa                                                                                                                              >  2:3367445/1‑140 (MQ=255)
               ctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCGTTTCCGTTTTTAAAACAag                                                                                                                             >  2:1934825/1‑140 (MQ=255)
               ctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGGTTTACGTTTTTAAAACAag                                                                                                                             >  2:589966/1‑140 (MQ=255)
                  aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTTAGTTTTTGGAGATTTTAATGGGTTTTGCGTTTTTAAAAAAAGAgg                                                                                                                          >  1:138522/1‑140 (MQ=255)
                  aTGTTGGAATTAGTCAGAGATGCTGTTTTTGTCTTTTTGTTCTACTTCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAGGGCTTTTCCGTTTTTAAAACAAGAgg                                                                                                                          <  2:863525/140‑1 (MQ=255)
                  aTGTCGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGGTCTAAGACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTGGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAgg                                                                                                                          <  1:711218/140‑1 (MQ=255)
                       ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATTGGTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                                     >  1:1844051/1‑140 (MQ=255)
                       ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAATTTTTGGAGATTTTAATTGCTTTTACGTTTTTAAAACAAGAGGCaatc                                                                                                                     >  1:49255/1‑138 (MQ=255)
                       ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTTGGAGATTTGAAATGCTTTTCCGTTTTTAAAAAAagatgaaaac                                                                                                                     >  2:1880153/1‑133 (MQ=255)
                       ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAATAGAATAGGCAAGc                                                                                                                     >  2:2324750/1‑140 (MQ=255)
                       ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAAAAAGAGGCAAGc                                                                                                                     >  1:790673/1‑140 (MQ=255)
                          attaatCAGCGATGCTCTTGTTGTAGTTTTGTTCGACTACGAAGTCGATCATTCCGACTATACGGGCCTTTTCGGATTTTTGCTTGAAGTTTTGGGCGATTTGAATGGCTTTTCCGTTTGTCAAACAAGAGGCAAGCCAg                                                                                                                  <  2:3480049/140‑1 (MQ=255)
                             attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                                               <  1:2736187/140‑1 (MQ=255)
                                cAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAAc                                                                                                            <  1:1699117/140‑1 (MQ=255)
                                   ataTGCTGTTTTTGTATTTTTGTTATAATAAGAAGTAGATAATTAATAATATACTTTAGTTTTATTTTTTTTTTTTTAAGTTTTGGGATATTTGAATGGCTTTTCAGTTTTTTAATAAAGATGCAAGCCAGATaaaacag                                                                                                         >  2:139448/3‑136 (MQ=255)
                                   agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGGGATTTTAATTGCTTTTGCGTTTTTAAAAAAAGAGGCAAACCACAAAAAaccc                                                                                                         >  1:1311090/1‑139 (MQ=255)
                                   agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTGGGAGATTTGAATGGCGTTTCCGTTTTTAAAACAAGAGGCAAGCCCGATAAACCCg                                                                                                         >  2:1989225/1‑140 (MQ=255)
                                   agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGGTTTGCGTTTTTAAAAAAAGAGGCAAGCCAGATAAAaccc                                                                                                         >  1:2406883/1‑139 (MQ=255)
                                   agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTGTCCGATTTTTGTTTGAAATTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAAAGGCAACCCAGATAAAccag                                                                                                         >  2:3104380/1‑138 (MQ=255)
                                     aTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTTAATTGCTTTTCCGTTTTTAAAACAATACGCAAGCCAGATAAACCCGac                                                                                                       >  2:2544718/1‑140 (MQ=255)
                                        cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAATTTTTGGAGATTTTAATTGGGTTTACGTTTTTAAAAAAAGAGGCAAGCCCCATAAACACCacaac                                                                                                    >  1:2144736/1‑140 (MQ=255)
                                        cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                                                                    >  2:1813685/1‑140 (MQ=255)
                                        cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAATAGGCAAGCCAGATAAACCCGacaac                                                                                                    >  2:2394220/1‑140 (MQ=255)
                                        cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGTCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                                                                    >  2:747092/1‑140 (MQ=255)
                                        cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCGaaaac                                                                                                    >  2:1858428/1‑140 (MQ=255)
                                        cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                                                                    >  2:227498/1‑140 (MQ=255)
                                            ttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaacaaca                                                                                                <  1:985714/140‑1 (MQ=255)
                                                     tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAATCCAGATAAACCCGACCACAACAAAAaataat                                                                                       >  2:2817592/1‑140 (MQ=255)
                                                     tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCACATAAACCCGACAACAACAAAAaataat                                                                                       >  2:2369741/1‑140 (MQ=255)
                                                     tttGTTCTAAGACGAAGTCGCTCAGTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTGTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                                       <  2:2217268/140‑1 (MQ=255)
                                                         ttCTAATACTAAGTCGATCATTCCGACTATACGTGCGTTTTCGGATTTTTGTTTGAAGTTGTGGGAGATTTGAAGGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                                   <  1:3539490/140‑1 (MQ=255)
                                                         ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                                   <  1:3824574/140‑1 (MQ=255)
                                                         ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                                   <  2:935298/140‑1 (MQ=255)
                                                          tCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGCTTTTTTTTTGTAGTTTTTGGTGATTTGTATGGGGTTTCCGTTTTTTAAACAAGGGGCAACGCAGCTAAACCCCCCCACCACCAAAAATAATCtgag                                                                                  >  1:1057597/1‑137 (MQ=255)
                                                               tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                             <  2:3281244/140‑1 (MQ=255)
                                                               tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                             <  1:2636821/140‑1 (MQ=255)
                                                               tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                             <  1:3320691/140‑1 (MQ=255)
                                                               tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                             <  1:3227543/140‑1 (MQ=255)
                                                                 cGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACGCGACAACAACAAAAAATAATCTAGCAAGTaaa                                                                           <  2:1215522/140‑1 (MQ=255)
                                                                    aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                                        <  2:1193451/140‑1 (MQ=255)
                                                                      tCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCGTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCca                                                                      >  2:3191799/1‑140 (MQ=255)
                                                                        gATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATAGGCAAGCCAGATAAAACCGACAACAAAAAAAAATAATCTAGCAAGTAAACTCcatg                                                                    >  2:3320691/1‑140 (MQ=255)
                                                                          tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                                  <  1:1030922/140‑1 (MQ=255)
                                                                          tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                                  <  2:544103/140‑1 (MQ=255)
                                                                          tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                                  <  2:1171056/140‑1 (MQ=255)
                                                                          tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                                  <  2:49255/140‑1 (MQ=255)
                                                                          tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                                  <  2:2589629/140‑1 (MQ=255)
                                                                          tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                                  <  1:3027827/140‑1 (MQ=255)
                                                                          tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                                  <  1:3290508/140‑1 (MQ=255)
                                                                          tCAGTCCGACTATCCGGTCGTTGTCGGATTTTTGTTTGCAGTTTTGTGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                                  <  1:2436627/140‑1 (MQ=255)
                                                                                cGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTACATGCATGGGGt                                                            >  2:2067731/1‑140 (MQ=255)
                                                                                      taCGGGCGTTTTCGGATTTTTGTTTGAATTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGAAAGTAAACTACATGCATGGGGTAattca                                                      >  2:2752508/1‑140 (MQ=255)
                                                                                      taCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGATATTTGAATGGGTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAattca                                                      >  2:3628230/1‑140 (MQ=255)
                                                                                      taCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTATCAAGTAAACTCCATGCATGGGGTAattca                                                      >  2:2864215/1‑140 (MQ=255)
                                                                                      taCGGGCGTGTTGGGATTTTTTTTTGAAGTTTTGGGTGATTTGAATGGGTTTTCCGTTTTTTAAAAAAGAGGCAAGCCAGATAAACCCCCCAACAACAAAAAAAAAACTAGCAAGTAAACTCCAAGCATGGGGTAattga                                                      >  1:1149865/1‑138 (MQ=255)
                                                                                       aCGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAAAGGCTTTTCCCTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCGACAAAAACAAAAAATAATCTAGCAAGTAAACTCCATGCAGGGGGTAattcag                                                     >  2:2990071/1‑139 (MQ=255)
                                                                                       aCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTcc                                                                                                                                           >  2:2165657/1‑54 (MQ=255)
                                                                                       aCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAattcat                                                     <  2:835344/140‑1 (MQ=255)
                                                                                              ttttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATATAGCAAGTAAAATCCATGCATGGGGTAAATCATTCGTTGc                                              >  2:876316/1‑140 (MQ=255)
                                                                                                ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGGAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                            <  1:3591020/140‑1 (MQ=255)
                                                                                                ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                            <  2:2509411/140‑1 (MQ=255)
                                                                                                ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAGGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                            <  2:108664/140‑1 (MQ=255)
                                                                                                ttCGGATCTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                            <  1:3777781/140‑1 (MQ=255)
                                                                                                    gATTTTTGTTTGAAGTTTTGGGAGATTTGACTGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                                        <  1:3098689/140‑1 (MQ=255)
                                                                                                          tgtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaaa                                  <  2:2710098/140‑1 (MQ=255)
                                                                                                             ttgAATTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                               <  1:1934825/140‑1 (MQ=255)
                                                                                                             ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                               <  1:589718/140‑1 (MQ=255)
                                                                                                             ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                               <  1:1449446/140‑1 (MQ=255)
                                                                                                             ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                               <  1:1946038/140‑1 (MQ=255)
                                                                                                             ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                               <  1:2498575/140‑1 (MQ=255)
                                                                                                             ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGAAAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                               <  2:1744281/140‑1 (MQ=255)
                                                                                                                       gggAGATTTTAAGGGCTTTTCCGTTTATAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAt                     <  2:318078/140‑1 (MQ=255)
                                                                                                                            aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc                <  1:2084653/140‑1 (MQ=255)
                                                                                                                            aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc                <  2:1134782/140‑1 (MQ=255)
                                                                                                                             tttGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCg               <  1:1291953/140‑1 (MQ=255)
                                                                                                                             tttGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCg               >  2:3217520/1‑140 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAACCCGACTACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTAGTTGCAAAATATAAAAATATAGTTACTAATGTGCCGTAGATTAc       <  2:3595514/140‑1 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCGGTAGATTAc       <  1:2361515/140‑1 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTAc       <  2:1028010/140‑1 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTAc       <  2:757520/140‑1 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTAc       <  1:2532814/140‑1 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTAc       <  2:3043734/140‑1 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTAc       <  2:467586/140‑1 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTAc       <  2:3835753/140‑1 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTAc       <  1:2004985/140‑1 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTAc       <  1:227650/140‑1 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTAc       <  2:1563724/140‑1 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCAGTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTAc       <  2:1584160/140‑1 (MQ=255)
                                                                                                                                     gCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCAGGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTAc       <  2:676203/140‑1 (MQ=255)
                                                                                                                                          tCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTACTGttt  <  1:3669098/140‑1 (MQ=255)
                                                                                                                                          tCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTACTGttt  <  1:290206/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGATTACTGTTT  >  NZ_CP034943/240079‑240356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: