BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	293,858	A→C	7.4%	N79T (AAT→ACT)	EO946_RS01645 →	aldo/keto reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	293,858	0	A	C	7.4%	551.3 / 5.7	266	N79T (AAT→ACT)	EO946_RS01645	aldo/keto reductase
Reads supporting (aligned to +/- strand): ref base A (136/96); new base C (32/2); total (168/98)
Fisher's exact test for biased strand distribution p-value = 1.66e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

TAAATGATGAAACAGGGAAAGATTTGGTGCGCACGGCATTGGATGGCGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACAGATTACATTGATTTGTATTA  >  NZ_CP034943/293720‑293996

tAAATGATGAAACAGGGAAAGATTTGGTGCGCACGGCATTGGATGGCGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAAt                                                                                                                                           >  1:3445422/1‑140 (MQ=255)

    tgatgaAACAGGGAAAGATTTGGTGCGCACGGCATTGGATGGCGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGc                                                                                                                                       <  2:2516071/140‑1 (MQ=255)

            cAGGGAAAGATTTGGTGCGCACGGCATTGGATGGAGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCattatt                                                                                                                               <  2:2623445/140‑1 (MQ=255)

                 aaaGATTTGGTGCGCACGGCATTGGATGGCGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGccac                                                                                                                          <  2:1349995/140‑1 (MQ=255)

                          gTGCGCACGGCATTGGATGGCGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGc                                                                                                                 <  2:3511275/140‑1 (MQ=255)

                          gTGCGCACGGCATTGGATGGCGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGc                                                                                                                 <  1:3605903/140‑1 (MQ=255)

                          gTGCGCACGGCATTGGATGGCGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGc                                                                                                                 <  1:2012345/140‑1 (MQ=255)

                               cACGGCATTGGATGGCGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCACAGGGGCTCATa                                                                                                            >  2:1368682/1‑140 (MQ=255)

                               cACGGCATTGGATGGCGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATa                                                                                                            >  1:3499207/1‑140 (MQ=255)

                               cACGGCATTGGATGGCGGCGTGAACTTTATTGATACCGCCTGTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATa                                                                                                            >  1:3281538/1‑140 (MQ=255)

                               cACGGCATTGGATGGCGGCGTCAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGATGACTAAATCGGGGACGTAGTGCACGAAAGAGGCGTGAGGACTCAGATCATGATTGCCACAAAAGGGGcgccga                                                                                                            >  2:2224158/1‑135 (MQ=255)

                                  ggCATTGGATGGCGGCGTGAACTTTAGTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAgg                                                                                                         <  1:3110093/140‑1 (MQ=255)

                                    cATTGGATGGCGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGaa                                                                                                       <  1:1353295/140‑1 (MQ=255)

                                    cATTGGATGGCGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGaa                                                                                                       >  1:1531590/1‑140 (MQ=255)

                                            ggcggcGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGACGTTGTGCAAGAACGAGGCGTGCGGACTGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACgg                                                                                               >  2:2034853/1‑140 (MQ=255)

                                            ggcggcGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGACTGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACgg                                                                                               >  2:1111446/1‑140 (MQ=255)

                                            ggcggcGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACgg                                                                                               <  1:2434663/140‑1 (MQ=255)

                                            ggcggcGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACgg                                                                                               >  1:912627/1‑140 (MQ=255)

                                            ggcggcGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACgg                                                                                               <  1:177131/140‑1 (MQ=255)

                                            ggcggcGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACgg                                                                                               >  2:33657/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGACTTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  2:3326805/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGACTGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  2:3824157/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGcc                                                                                                                            >  1:3276494/1‑109 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGACCGgca                                                                                             >  1:3763379/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGACCGgca                                                                                             >  2:3815745/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  1:3837750/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  1:810962/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  1:3534973/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  2:2180840/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  2:1666789/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  1:1928899/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  2:3553054/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  1:1467547/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  2:3677631/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  1:1576197/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  1:2497528/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgca                                                                                             >  2:678786/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCAACCAAAGTGGCTAATAAGGAAGTGAACGgca                                                                                             >  1:2899866/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTTTATATAAGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAACTGAACGgca                                                                                             >  1:1262209/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATACCGCCTGTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGACTGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGACCGgca                                                                                             >  1:431605/1‑140 (MQ=255)

                                              cggcgTGAACTTTATTGATAACGCCAATATAAACGGAATGGGACGATCTGACGAATTAATCTGGCAAGATCTGCAAAAAACATACGAACGGAATGAGCTGATTATTGCCACCACAGGGGCTGATAAGGAAGTGAACGgca                                                                                             >  1:2287480/1‑140 (MQ=255)

                                                 ggTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgcagca                                                                                          <  2:1959343/139‑1 (MQ=255)

                                                 cgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgcagca                                                                                          <  1:1666321/140‑1 (MQ=255)

                                                 cgTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGgcagca                                                                                          <  1:577057/140‑1 (MQ=255)

                                                   tGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGACTGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCAtt                                                                                        >  1:3544824/1‑140 (MQ=255)

                                                   tGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGaa                                                                                                  <  2:2449437/130‑1 (MQ=255)

                                                   tGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCAtt                                                                                        >  1:2992784/1‑140 (MQ=255)

                                                   tGAACTGTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCAtt                                                                                        >  1:950590/1‑140 (MQ=255)

                                                    gAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTACTCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTg                                                                                       <  1:931995/140‑1 (MQ=255)

                                                       cTTTATTGATACCGCCTTTATAGACGGACTGGGACGGTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAAt                                                                                    <  2:3870269/140‑1 (MQ=255)

                                                                 aCCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAg                                                                          >  1:1939112/1‑140 (MQ=255)

                                                                 aCCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAg                                                                          <  1:2123450/140‑1 (MQ=255)

                                                                  ccGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCattatt                                                                                                                               <  2:2104969/86‑1 (MQ=255)

                                                                  ccGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGACTTAGACAACAGc                                                                         >  2:1190759/1‑140 (MQ=255)

                                                                  ccGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGAAATAAAGATGCGTGCGGACTGAGCTCATTATTGCCACCAAAGGGGCTCGTAAGGAAGTTAACTGCCGCATTGACTTAGACAACAGc                                                                         >  2:3170403/1‑140 (MQ=255)

                                                                        ttATATACGGACTGGGACGTTCTGATGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTAGTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGaaa                                                                   <  1:1007249/140‑1 (MQ=255)

                                                                                ggACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGACTGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTcg                                                           >  2:1452225/1‑140 (MQ=255)

                                                                                  aCTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGACCGGCAGCATTGAATTAGACAACAGCAGGAAATTTCTTcgcg                                                         >  2:3273872/1‑140 (MQ=255)

                                                                                  aCTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTcgcg                                                         >  2:2993072/1‑140 (MQ=255)

                                                                                  aCTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTcgcg                                                         >  2:3625923/1‑140 (MQ=255)

                                                                                  aCTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTcgcg                                                         >  2:988278/1‑140 (MQ=255)

                                                                                  aCTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTcgcg                                                         >  2:1056441/1‑140 (MQ=255)

                                                                                     gggACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCgcggcg                                                      <  1:2165925/140‑1 (MQ=255)

                                                                                     gggACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCgcggcg                                                      >  1:36873/1‑140 (MQ=255)

                                                                                         cGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGt                                                  <  2:884602/140‑1 (MQ=255)

                                                                                           ttCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAg                                                >  1:3321215/1‑140 (MQ=255)

                                                                                           ttCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAg                                                >  1:706743/1‑140 (MQ=255)

                                                                                           ttCTGAGGAATGAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCAGAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGCGGTAg                                                >  1:3023710/1‑140 (MQ=255)

                                                                                            tCTGTGGAATTAGTCGGGGCAGTTGTGCAAGAACGAGGCGTGCGGACGGAGCTCCTTCTTGCCCCCAAGGGGGCTCAGAAGGACGTGACCGGCAGCAGTGACTTAGGCAGCACCCGGAGCTTTGTGCGCGGCGGGGTCGa                                               >  2:599383/1‑140 (MQ=255)

                                                                                            tCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAAt                                                                                                                                           >  2:1582970/1‑48 (MQ=255)

                                                                                            tCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAAt                                                                                                                                           <  1:1582970/48‑1 (MQ=255)

                                                                                            tCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCACAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGa                                               >  2:2376266/1‑140 (MQ=255)

                                                                                            tCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGacaa                                                                             >  1:3216942/1‑110 (MQ=255)

                                                                                            tCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTCGa                                               >  2:1659235/1‑140 (MQ=255)

                                                                                            tCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGa                                               >  2:2663626/1‑140 (MQ=255)

                                                                                            tCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGa                                               >  1:2921839/1‑140 (MQ=255)

                                                                                                aGGACTTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAag                                           >  2:1330551/1‑140 (MQ=255)

                                                                                                     ttAATCGGGGAAGTTTTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCAGAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGCAAATTTCTTCGCTCCGATGTAGAAAAGAGACt                                      >  1:3466392/1‑140 (MQ=255)

                                                                                                     ttAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAg                                                                                                                                        >  2:934015/1‑42 (MQ=255)

                                                                                                     ttAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGACATTTCTTCGCGGCGAGGTAGAAAAGAGCCt                                      >  2:1504905/1‑140 (MQ=255)

                                                                                                     ttAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCt                                      >  2:3224031/1‑140 (MQ=255)

                                                                                                     ttAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCt                                      >  2:3770257/1‑140 (MQ=255)

                                                                                                     ttAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCt                                      >  2:579745/1‑140 (MQ=255)

                                                                                                     ttAATCGGGGAAGTTGTGCAAGAAATATTAGTTCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTCGAAAAGAGCCt                                      >  2:3401125/1‑140 (MQ=255)

                                                                                                         tCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGTCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAg                                  <  2:305579/140‑1 (MQ=255)

                                                                                                             ggAAGTTGTGCAAGAACGAGGCGTGCGGACTGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGc                              >  2:2701884/1‑140 (MQ=255)

                                                                                                             ggAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGc                              >  2:1270150/1‑140 (MQ=255)

                                                                                                             ggAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAAGTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGc                              >  2:2192483/1‑140 (MQ=255)

                                                                                                                  ttGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACACCAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGaaa                         <  2:652922/140‑1 (MQ=255)

                                                                                                                     tgCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACa                      <  1:3112546/140‑1 (MQ=255)

                                                                                                                     tgCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACa                      <  1:2654180/140‑1 (MQ=255)

                                                                                                                       cAAGAACGAGGCGTGCGGACTGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGAGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACAGa                    <  1:2034853/140‑1 (MQ=255)

                                                                                                                            aCGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACAGATTACa               <  1:1056441/140‑1 (MQ=255)

                                                                                                                            aCGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACAGATTACa               <  1:274209/140‑1 (MQ=255)

                                                                                                                            aCGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACAGATTACa               <  2:3445422/140‑1 (MQ=255)

                                                                                                                                 gCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACAGATTACATTGAt          <  2:3504942/140‑1 (MQ=255)

                                                                                                                                 gCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACAGATTACATTGAt          <  2:1990401/140‑1 (MQ=255)

                                                                                                                                 gCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACAGAGTACATTGAt          <  2:288164/140‑1 (MQ=255)

                                                                                                                                   gTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACAGATTACATTGAttt        <  1:3557113/140‑1 (MQ=255)

                                                                                                                                      cGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACAGATTACATTGATTTGta     <  1:666997/140‑1 (MQ=255)

TAAATGATGAAACAGGGAAAGATTTGGTGCGCACGGCATTGGATGGCGGCGTGAACTTTATTGATACCGCCTTTATATACGGACTGGGACGTTCTGAGGAATTAATCGGGGAAGTTGTGCAAGAACGAGGCGTGCGGAATGAGCTCATTATTGCCACCAAAGGGGCTCATAAGGAAGTGAACGGCAGCATTGAATTAGACAACAGCCGGAAATTTCTTCGCGGCGAGGTAGAAAAGAGCCTGAAGCGGCTGAAAACAGATTACATTGATTTGTATTA  >  NZ_CP034943/293720‑293996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`