Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 316,300 G→C 8.9% L40L (CTG→CTC opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943316,3000GC 8.9% 596.4 / 6.7 278L40L (CTG→CTCopuAAglycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Reads supporting (aligned to +/- strand):  ref base G (129/102);  new base C (2/27);  total (131/147)
Fisher's exact test for biased strand distribution p-value = 1.92e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

TTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAACCGAC  >  NZ_CP034943/316162‑316437
                                                                                                                                           |                                                                                                                                         
       tgcaGGAAAAATATGAGTGTATATGATAAACCAATTAACATAAAAGTCGAGAAAGTATATAAAATTTTTGGGAAACAAACAAAGAAAGAAGTTCAAATGCTTGCCAACGTAAAGACAAAAAAATAGATCCTGAAAGCGAc                                                                                                                                   <  2:274066/137‑1 (MQ=255)
        ggAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGAcc                                                                                                                                  >  1:3843118/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAACGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCCACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  2:3764534/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGACAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  2:1205733/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACACAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  2:2642513/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGACAGCGACCgg                                                                                                                                >  2:841538/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:1534652/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:3501424/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  2:623520/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  2:1859235/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:1279763/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  1:1255869/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCGAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  2:1969097/1‑140 (MQ=255)
          aGGGAAAATATGAGTGTAGATGACAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACACACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                >  2:993894/1‑140 (MQ=255)
             gAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAACCAGTTCAAATGATTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATc                                                                                                                             <  2:758759/140‑1 (MQ=255)
               aaaTATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCaa                                                                                                                           >  2:1032312/1‑140 (MQ=255)
                   atGAGTGTCGATTACAAACCAATTAAGATAAATGTCGACAAAGTATCTAAAATTTTTGGTAAAAAAAAAAATAAAGCAGTTCAATTGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGt                                                                                                                       <  1:3664910/140‑1 (MQ=255)
                    tgtgtgtATATGAGAAACAAATTAATATAAAAGTCGAGAAATTAACTAAAATTTTTGGGAAACAAACAAATAAAGCAGTTAAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGtt                                                                                                                      <  2:3821628/137‑1 (MQ=255)
                       gtgtAGTTGATAAACAAATTAACATAAAAGTCCATAAAGTATCTAAAATTTTTGGCAAAAAAAAAAACAAAGCAGTTCAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTggg                                                                                                                   <  2:1426755/140‑1 (MQ=255)
                        tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                                  >  1:1426755/1‑140 (MQ=255)
                        tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                                  >  2:444410/1‑140 (MQ=255)
                                agaAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCaa                                                                                                          >  2:2206316/1‑140 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCg                                                                                                                        >  1:3804957/1‑124 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCg                                                                                                                        <  2:3804957/124‑1 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                        >  2:1640235/1‑140 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                        >  2:1122823/1‑140 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                        >  1:562495/1‑140 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGc                                                                                                        >  1:248314/1‑140 (MQ=255)
                                  aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGACAACGGAAAGACAATAAAATACATCCTCAAAGCGACCTCATCAAGCGTAGGGGAAAATCAAGc                                                                                                        >  2:2097279/1‑140 (MQ=255)
                                  aaaCAATTTAATAAAAAAGCCGAGAAAGTATATAAAATTTTTGGCAAACAAAAAAAGAAAGCCGTAAAAAGGGTTGCCAACGCAAAGACAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                        <  1:3733780/140‑1 (MQ=255)
                                    aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCACGCAg                                                                                                      >  2:713773/1‑140 (MQ=255)
                                    aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAg                                                                                                      >  2:1943841/1‑140 (MQ=255)
                                        aTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAttt                                                                                                  >  1:98575/1‑140 (MQ=255)
                                        aTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAttt                                                                                                  >  1:1002268/1‑140 (MQ=255)
                                         ttAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtttt                                                                                                 >  1:2086034/1‑140 (MQ=255)
                                         ttAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtgtt                                                                                                 >  2:2776846/1‑140 (MQ=255)
                                          tAATATAAACGTCTACAAAGTATCTAAAATTTTTGGCAAACAAAAAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                                                <  2:1097728/140‑1 (MQ=255)
                                          tAATATAAAAGTCGAGAAAGTATCTAAAATTTGTGCGAAAAAAACAAAGACAGCATTACAACTGCTTGCCACCGGAACCAAAAAAAAAGAGCCCCTCCACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                                                <  1:768079/140‑1 (MQ=255)
                                            acaTAAACGTCGAGAAAGTATCTAAATTTTTTGGCAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCACCGCAACGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                                              <  2:1739213/138‑1 (MQ=255)
                                            aGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                                              <  2:1260693/140‑1 (MQ=255)
                                             gATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACAGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATg                                                                                             >  2:3147140/1‑140 (MQ=255)
                                             gATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATACTGAAAGCGACCGGATCAACCGTTGGGGTAAATAAAGCAGATTTTGATg                                                                                             >  2:2008737/1‑140 (MQ=255)
                                                aaaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAATAAGATTTTGATGtat                                                                                          >  2:3261576/1‑140 (MQ=255)
                                                 aaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACACAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtata                                                                                         >  1:2326671/1‑140 (MQ=255)
                                                 aaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtata                                                                                         >  1:389024/1‑140 (MQ=255)
                                                 aaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtata                                                                                         >  1:1323403/1‑140 (MQ=255)
                                                   aGTCGATAAATTATCTAAAATTTTTGGTAAAAAAACAAAGAACGCAGTTCAAATGGTTGCCAACGGAAACAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatg                                                                                       <  2:3519152/140‑1 (MQ=255)
                                                       tagaAATTTTCTAAAATTTTTGGGAAACAAACAAATAAAGCAGTTCAAATGATTGCCAACGGAAACAAAAAAAAAGAGATCCGTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                                                                   <  2:2562149/139‑1 (MQ=255)
                                                       gagaAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                                                                   >  1:85012/1‑140 (MQ=255)
                                                        agaAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAATTGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGt                                                                                  <  2:2240825/140‑1 (MQ=255)
                                                        agaAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGt                                                                                  >  2:2856371/1‑140 (MQ=255)
                                                        agaAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGt                                                                                  >  2:3338178/1‑140 (MQ=255)
                                                        agaAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGt                                                                                  >  2:1314147/1‑140 (MQ=255)
                                                               aTCTAAAATTTTGGGACAAAAAACAAATAACGCAGTTCAAATGGTTGCCACCGGAAAGAAAAAAAAATATATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                                                           <  2:523394/140‑1 (MQ=255)
                                                                 atAAAATTTTTGGCAAACAAACAAATAAAGAAGTTCAAATGCTTGCAAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                                                                         <  2:3495339/139‑1 (MQ=255)
                                                                   aaatTTTTTGGCAAAAAAACAACTAAAGCAGTAAAACGGGTTGCCAACGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGt                                                                       <  2:488646/140‑1 (MQ=255)
                                                                       tttttGGTAAACAAAAAAACAAACCCTTACAAAGCGTTCCAAAGCCAAACAAAAAAAAACACAGCGTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGCTGAGATATTTGtcatc                                                                   <  1:2156803/140‑1 (MQ=255)
                                                                       tttttGGCAAACAAACAAATAAAGCAGATCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatc                                                                   <  2:3579698/140‑1 (MQ=255)
                                                                        ttttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatca                                                                  >  2:2083806/1‑140 (MQ=255)
                                                                         tttgtcaaaaaaacAAACAACCCCTTTAAAAGGCTTGCCACCGCAAACAAAAAAAAAGCGACCCGAAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                 <  1:1032312/134‑1 (MQ=255)
                                                                         tttGGCAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGCAACCAAAAAAAAAGAGCTCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                 <  2:2441528/140‑1 (MQ=255)
                                                                         tttGGCAAAAAAACAAAGAAAGCAGTTAAAATGCTTGCAAACGCAAAGACAAAAAAAGAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                 <  2:3424484/140‑1 (MQ=255)
                                                                           tGGGAAACAAACAAAGAAAGCAGTTCAACTGCTTGCCAACGCAAAGACAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                                                               <  2:2799637/140‑1 (MQ=255)
                                                                           tGGCAAACAAACAAAGAACCAAGTCCAATTGGTTCCCAACGTAAAGACAAAAAAACATATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                                                               <  1:1411315/140‑1 (MQ=255)
                                                                           tGGCAAAAAAACAAACAAACCCGTAAAATTGCTTCCCACCGCAAACACAAAAAAAGACATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                                                               <  1:1615524/140‑1 (MQ=255)
                                                                           tGGCAAAAAAAAAAAGAAAGCAGTTCAAAGGCTTCCCAACGCAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                                                               <  1:3610533/140‑1 (MQ=255)
                                                                           tGGAAAACAAACAAATAACGCCGTAAAAATTGTTGCCAACGTAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGAGATTTGTCATCATgg                                                               <  1:1075895/140‑1 (MQ=255)
                                                                           ggggACACACACAAAGAAAGCAGTTCACATGCTTGCCAACGGAAACCCAAAAAAAGATATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                                                               <  2:1336416/139‑1 (MQ=255)
                                                                             gtaaacaaacAAAGAAAGCCGTTCAAATGCTTGCCAACGCAAAGACAAAAAAAGCGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                                                             <  2:540364/138‑1 (MQ=255)
                                                                              taaaaaaaaaaAGAAAGCATTTAAAAGGTTTGCCAACGCAAACACAAAAAAAGAGCCCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTc                                                            <  1:1186179/139‑1 (MQ=255)
                                                                                      aaaataaagCAGTTAAAAGGCTTGCCAAGGGAACGACAAAAAAATAGCACTTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAggg                                                    <  2:971082/139‑1 (MQ=255)
                                                                                                aGTTCAATTGCTTGCCAAGGGAAACACAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAAt                                          <  2:2326071/140‑1 (MQ=255)
                                                                                                 gTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc                                         >  1:3059365/1‑140 (MQ=255)
                                                                                                 gTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc                                         >  1:922614/1‑140 (MQ=255)
                                                                                                 gTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc                                         <  2:1488046/140‑1 (MQ=255)
                                                                                                 gTTAAAATGGTTGCCAACGTAAACAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc                                         <  1:2787768/140‑1 (MQ=255)
                                                                                                 cttCAAATGCTTGCCAACGGAAACACAAAAAAACAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc                                         <  1:301407/139‑1 (MQ=255)
                                                                                                     aaaTGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACt                                     >  2:804694/1‑140 (MQ=255)
                                                                                                            tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                              >  1:2913542/1‑140 (MQ=255)
                                                                                                            tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                              >  1:2342632/1‑140 (MQ=255)
                                                                                                            tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                              >  1:2384810/1‑140 (MQ=255)
                                                                                                            tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                              <  1:2657800/140‑1 (MQ=255)
                                                                                                            tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                              >  1:3265015/1‑140 (MQ=255)
                                                                                                            tGCCAACGCAAACAAAAAAAAAGACATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                              <  1:2427116/140‑1 (MQ=255)
                                                                                                             gcaaaCGCAAACACAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACg                             <  1:3343877/137‑1 (MQ=255)
                                                                                                             gCCAACGTACAGACAAAAAAAGACATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACg                             <  1:1893954/140‑1 (MQ=255)
                                                                                                                  cGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGt                        >  1:2758743/1‑140 (MQ=255)
                                                                                                                      aaaGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTaa                     >  2:2902284/1‑140 (MQ=255)
                                                                                                                       aaGAAAAAAAAAGAGCTCCTTAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTaaa                    <  2:1440466/140‑1 (MQ=255)
                                                                                                                        aGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAAc                   >  2:1242474/1‑140 (MQ=255)
                                                                                                                          aCAAAAAACGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCg                 <  1:3568302/140‑1 (MQ=255)
                                                                                                                          aCAAAAAAAGAGATCCGAAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCg                 <  2:1299559/140‑1 (MQ=255)
                                                                                                                            aaaaaaaGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGc               <  2:302437/140‑1 (MQ=255)
                                                                                                                            aaaaaaaGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGc               <  2:248010/140‑1 (MQ=255)
                                                                                                                            aaaaaaaGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGc               <  2:2342632/140‑1 (MQ=255)
                                                                                                                                     gaTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAAc      >  1:1862562/1‑140 (MQ=255)
                                                                                                                                     gaTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAAc      >  1:2707118/1‑140 (MQ=255)
                                                                                                                                        ccTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAACCGa   <  1:2054179/140‑1 (MQ=255)
                                                                                                                                         cTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAACCGAc  <  2:2820142/140‑1 (MQ=255)
                                                                                                                                         cTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAACCGAc  <  2:1319695/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAACCGAC  >  NZ_CP034943/316162‑316437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: