Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943177,0620CA24.9% 453.7 / 33.9 233intergenic (+235/‑37)EO946_RS01020/EO946_RS01025alcohol dehydrogenase/SulP family inorganic anion transporter
Reads supporting (aligned to +/- strand):  ref base C (77/98);  new base A (58/0);  total (135/98)
Fisher's exact test for biased strand distribution p-value = 2.60e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 233 total aligned reads displayed.

GATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATT  >  NZ_CP034943/176925‑177200
                                                                                                                                         |                                                                                                                                            
gATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACgg                                                                                                                                            <  1:1765818/140‑1 (MQ=255)
      aaTTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGAGTCCTCTTTTTCTCCGGTCTTGTTGGTTTGATTTATTCCGTAAAATAAATAAAACTCACCCAACAACAAATAAgggtgggt                                                                                                                                      >  1:119518/1‑134 (MQ=255)
                gATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTACCACTACAACAAAAAAGGTGGGGATTTAtttttt                                                                                                                            >  2:4055520/1‑140 (MQ=255)
                 aTACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAACCTCCCAATACAACAAATAAGGGGCGGATTTATTTTTTa                                                                                                                           >  2:3252157/1‑140 (MQ=255)
                 aTACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTAATTTCTCCCGTAAAATAAAGAAAGCTCCCAATAAAACAAATACCGTGCGGCTTTATTTTTTa                                                                                                                           >  2:1845153/1‑140 (MQ=255)
                 aTACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTCTCCCGTAAAATAAATAAATCTCCCAATAAAAAAAAAACGGTGCGGATTTATTTTTTa                                                                                                                           >  2:2593819/1‑140 (MQ=255)
                      ctTTGTATAATAACTCCTTGCCTAAAATAAACATCCTCACCGTTTCCTCTTTTTCTCCGCTCGTTTTCCTTTCATTTCTCCCGTAATATAAAGAAATCTCCCAATACAACACATCCGGTGCGGATTTATTTTTTAAAAAg                                                                                                                      <  1:2421406/140‑1 (MQ=255)
                       tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATCCCGTAAAATAAAGAAAGCTCCCAATACAACAAAAACGGGGCGGATTTATTTTTTAAAAAgg                                                                                                                     >  2:4512398/1‑140 (MQ=255)
                       tttGTATAATAACTCCTTGCCGCAAAACAACATACTCAACGTGTCCTCTTGTTCTCCGGTCGTTTTGCTGTCATTTGTCCCGGTAAAAAAAGGACTGTGCCCATAACACACCTACGGTGCGGCTTTATTTTTTaaaaatg                                                                                                                     >  1:2127789/1‑138 (MQ=255)
                         tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAAAAAATAAGGTGCGGAGTTATTTTTTAAAAAGGAg                                                                                                                   >  2:2846746/1‑140 (MQ=255)
                         tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCGGTCATTTCTACCGTAAAATTAAGAAAGCGCCCAAAACAACAAATACCGAGGGGCATTATTTTTTAAAAATGAg                                                                                                                   >  2:2768952/1‑140 (MQ=255)
                         tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTCTCCCGTAAAAAAAATAAAGCTCCCAATACAACAAATAAGGTGCGGATTTATTTTTTAAAAAGTAg                                                                                                                   >  2:864963/1‑140 (MQ=255)
                          gTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTCTCCCGTAAAATAAAGAAAGCTACCAATACAAAAAATACGGTGCGGATATATTTTTTAAAAATGAGt                                                                                                                  >  2:1249877/1‑140 (MQ=255)
                           tataATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGAATTGTCCCGTAAAATAAAGAAAGCTGCCAAAACAACAAATACGGTGCGGATTTATTTTTTaaaaatgtgtg                                                                                                                 >  1:3467011/1‑134 (MQ=255)
                            ataataACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTGATTTATCCCGTAAAATAAAAAAAGCTCCCAAAAAAAAAAAAACGGTGCGGGTTTATTTTTTAAAAATGAGtgg                                                                                                                >  1:3251978/1‑138 (MQ=255)
                             taataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAAGACGGTGCGGATTTATTTTTTAAAAAGGAGTCGt                                                                                                               <  1:4090746/140‑1 (MQ=255)
                             taataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGt                                                                                                               <  1:3024932/140‑1 (MQ=255)
                               ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTGATTTGTCCCGTAAAATAAAGAAAGCTCCCAATAAAACAAAAAAGGGGCGGCTTTATTTTTTAAAAATGAGTGGttt                                                                                                             >  1:559130/1‑139 (MQ=255)
                               ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTCATTTGTGCCGTAAAATAAACAAACCGCCCCATACAAAAAAAACGGGGGGGGTTTATTTTTTAAAAATGAGTCggtt                                                                                                             >  1:330062/1‑137 (MQ=255)
                                taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTTCGTTGATTTGTCCCCGAAAATAAATAAAACTACCAAAACAACAAAAAAGGTGCGGGTTTTTTTTTTAAAAATGAGTGGtttt                                                                                                            >  1:2946953/1‑138 (MQ=255)
                                taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTTATCCCGTAAAATAAACAAAACTCCCAATAAAACAAATACGGTGCGGATTTATTTTTTAAACATGAGGGGTtat                                                                                                           >  2:4195882/1‑140 (MQ=255)
                                taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTCTCCCCTAAAATAAAGAAAGCTACCAATAAAACAAATAACGTGCGGATTTATTTTTTAAAAAGGATTCttttt                                                                                                            >  2:2821993/1‑138 (MQ=255)
                                 aaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTAATTTTTACCCTAAAAAAAAAAAAGCACCCAAAAAAAAAAAAAACGTGGGGATTTATTTTTTTAAAAAGTGTGGTttta                                                                                                          >  1:721827/1‑140 (MQ=255)
                                 aaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCGTTAATTTCTACCGTAAAAAAAATAAAACTCCCAAAACAACAAATACGGTTGGGGTTTATTTTTTTAAAAGGATTCGTtata                                                                                                          >  2:1998441/1‑140 (MQ=255)
                                  aCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTatat                                                                                                         <  2:1348187/140‑1 (MQ=255)
                                  aCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTatat                                                                                                         <  1:2060664/140‑1 (MQ=255)
                                   cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCGTTTCATTTATCCCCTAAAATAAAGAAAGCTCCCCAAACAACAAATACGGGGCGGCTTTATTTTTTAAAAAGGAGTGGTTAGAtt                                                                                                        >  2:3889218/1‑140 (MQ=255)
                                   cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACACATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATAtt                                                                                                        <  2:1205776/140‑1 (MQ=255)
                                   cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATCCCGTAAAATAAAGAAAGCTCCCAATACAAAAAATAAGGTGCGGATTTATTTTTTAAAAATGAGTCGTTATAtt                                                                                                        >  2:2364905/1‑140 (MQ=255)
                                   cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTAATTTCTACCGTAAAAAAAAGAAAGCTCCCAATACAAAAAATAAGGTGCGGATTTATTTTTTAAAAATGAGTCGGTATAtt                                                                                                        >  2:627093/1‑140 (MQ=255)
                                   cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACGTTCATTTATCCCGTAAAATAAAGAAAGCTCCCAATACAACAAAAACGGGGCGGATTTATTTTTTAAAAAGGAGGCGTTATAtt                                                                                                        >  2:949368/1‑140 (MQ=255)
                                   cTCCTTGCCTCAAAAAAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTGATTTAACCCCTAAAAAAAAGAAATCTCCCAAAAAAACAAAAAACTTGGGGCATTTTTTTTTGAAAATGATTCGtttaatt                                                                                                         >  1:681814/1‑135 (MQ=255)
                                     ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTTCTTTGATTTCTACCGTTAAATAAAGAAAGATACCAAAAAAAAAAAAAAGGGGCGGGTTTATTTTTTAAAAATGATTGGTGTTAtttt                                                                                                      >  1:2174280/1‑139 (MQ=255)
                                     ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGATTTGTCCCGTAAAATAAAGAAAGCTCCCCATACAACAAATACGGTGCGGGTTTATTTTTTTAAAATGAGTCGTTGTTTTTg                                                                                                      >  2:4005684/1‑140 (MQ=255)
                                     ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTGATTTATCCCGTAAAATAAAGAAAGCTCCCAATACAAAAAAAACCGTGCGGCTTTATTTTTTAAAAATGAGTGGTTTTATTTg                                                                                                      >  2:4126934/1‑140 (MQ=255)
                                     ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCACCCCTAAAATAAAGAAAGCTCCCAAAACAAAAAAAACGGTGCGGAATTAATTTTTAAAAAGGAGGCGGTATATTTg                                                                                                      >  2:1079814/1‑140 (MQ=255)
                                     ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATCCCGTAAAATAAAGAAAGCTCCCAATAAAACAAATACGGTGCGGATTTATTTTTTAAAAAGGATTCGTTATAtttt                                                                                                      >  2:2978642/1‑139 (MQ=255)
                                     ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTTATTTGTCCCGGAAAATAAATAAAACGCCCCAAACAAAAAATAAGGGGGGGGGTTTTTTTTTTAAAAGGAGTGGTTTTAtttt                                                                                                      >  1:343800/1‑139 (MQ=255)
                                          ccTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaataa                                                                                                 <  1:1354051/140‑1 (MQ=255)
                                              aaaaCAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCACAAAAAAACAAATAATGTGCGGATTTATTTTTTAAAAATGAGTGGTTATAATTTAataatatt                                                                                             >  2:3530546/1‑138 (MQ=255)
                                              aaaaCAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTatat                                                                                                         >  2:1166574/1‑128 (MQ=255)
                                              aaaaCAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCCAAACAAAAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTTAATAAAAGt                                                                                            >  2:735550/1‑140 (MQ=255)
                                                gagacagtACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGACAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                          <  1:1491119/133‑1 (MQ=255)
                                                aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                                                          <  2:1088132/140‑1 (MQ=255)
                                                     cctACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATACAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACAt                                                                                     <  1:458295/138‑1 (MQ=255)
                                                     caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTGATTTTTGCCGTAAAATAAAGAAAGCTCCCAATAAAAAAAATAAGGTGCGGATTTATTTTTTTAAAAGGAGTCGTTTTTTTTTTATAATAAtttttgat                                                                                     >  2:1815683/1‑136 (MQ=255)
                                                        aCTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGGGTCGTTATATTTTTATAATAGTTTTACATTaa                                                                                  >  2:545157/1‑140 (MQ=255)
                                                        aCTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTaa                                                                                  <  1:3971866/140‑1 (MQ=255)
                                                          tCAACGTTTCCTCTTTTTCTCCGGTAGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAag                                                                                <  2:1012592/140‑1 (MQ=255)
                                                          tCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAag                                                                                <  1:984309/140‑1 (MQ=255)
                                                            aaCGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTATGCCGTAAAATAAATAAATCTCCCAATAAAAAAAATAAGGTTCGGATTTATTTTTTAAAAATGAGTGGTTATTATTTAAATATATTTTTAAAATaaaaaa                                                                              >  2:795102/1‑137 (MQ=255)
                                                            aaCGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTAATTTGTCCCGTAAAATAAATAAATCTGCCCATAAAAAAAAGAAGGTTGGGGTTTTTTTTTTTAAAATGGGTGGGTGTTTTTTGATAATATTTTTTAATTaaaaga                                                                              >  1:1417677/1‑137 (MQ=255)
                                                             aCGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTGTCCCCTAAAATAAATAAAGCTCCAAATAAAACAAAAAAGGGGCGGAGTTATTTTTTTAAAATGAGTCGTTATATTTGTATAATAGTTTTTCATTAAagcat                                                                             >  2:2435564/1‑139 (MQ=255)
                                                             aCGTGTCCTCTTTTTCTCCGGTCGTGTTGGTTTGATTTGTCCCGGAAAATAAATAAATCTCCCAAAACAACAAAAAAGGGGGGGGGTTATTTTTTTAAAAGGCGTGGGTTTATTTTTTTAATATTTTTAAAATaaaagag                                                                             >  1:4679002/1‑136 (MQ=255)
                                                              cGTGTCCTCTTTTTCTCCGGTCGTTTTTCTGTGATTTGTGCCGTAAAAAAAATAAAGCTCCCAAAAAAACAAAAACGGTGGGGAGTTTTTTTTTTAAAAGGAGGGGTGTTATTTTTATAAAATGTTTTTAATTaaaaaac                                                                             >  1:2829629/1‑136 (MQ=255)
                                                              cGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTTCTCCCGTAAAATAAAGAAATCTCCCAATACAACAAATAAGGTGCGGATTTATTTTTTAAAAAGGAGTAGTTTTATTTGTATAATATTTTTAAAATAAaacagc                                                                            >  2:1537730/1‑140 (MQ=255)
                                                              cGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTGATTTCTCCCGTAAAATAAAGAAAGCTCCCAATAAAAAAAATAAGGTGGGGATTTATTTTTTAAAAAGGAGTGGTTTTTTTTGTATAATAGTTTTTAATTaaatgagc                                                                            >  2:2008008/1‑135 (MQ=255)
                                                              cGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAagcagc                                                                            <  2:3737008/140‑1 (MQ=255)
                                                              cGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATAAGGTGCGGATTTATTTTTTAAAAATGAGTCGTTATATTTGAATAATATTTTTAAATTAAagaatc                                                                            >  2:3306224/1‑136 (MQ=255)
                                                              cGTGTCCTCTTTTTCTCCGCTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCAAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAagcagc                                                                            <  1:4564340/140‑1 (MQ=255)
                                                                tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACCACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                          <  1:897404/140‑1 (MQ=255)
                                                                tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                          <  1:658267/140‑1 (MQ=255)
                                                                tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                          <  1:1137145/140‑1 (MQ=255)
                                                                tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                          <  2:1405952/140‑1 (MQ=255)
                                                                tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCAGTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                          <  2:4679002/140‑1 (MQ=255)
                                                                tgtCCTCCTTTGCTCCGGTCGTTTTCCTGTCAGTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAACTACGGTGCGGATTTATTTTTTAAAAACGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                                                          <  2:3703323/140‑1 (MQ=255)
                                                                 gtCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTTCTCCCGTAAAAAAAACAAAGCACCCAATACAAAAAAAACGGGGCGGATTTATTTTTTTAAAAGGATTGGGTATATTTGAATAATAATTTTAGATTAAATCAGCAAt                                                                         >  2:1379235/1‑140 (MQ=255)
                                                                 gtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAAt                                                                         <  1:2978642/140‑1 (MQ=255)
                                                                 gtCCTCTTTTTCTCCGGTCGTTTTCCTTTAATTTCTCCCGGAAAATAAAGAAAGCTCCCAAAACAACAAATACCGTGCCGCTTTTTTTTTTAAAAAGGATTAGTTATATTTTAATAATATTTTTAAAATAAAGCAGCaaa                                                                         >  2:4306041/1‑139 (MQ=255)
                                                                 gtCCTCTTTTTCTCAGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAAt                                                                         <  1:1079814/140‑1 (MQ=255)
                                                                  tCCTCTTTTTCTCCGGTCGTGTTTCTTTGATTTCTACCGTAAAAAAAAGAAATCTGCCAAAAAAAAAAATAAGGTGGGGGTTTATTTTTTAAAAAGGAGTGGTTATTGTTTTATAATATGTTTAAAATAAAGCAGCaaag                                                                        >  2:1641279/1‑138 (MQ=255)
                                                                  tCCTCTTTTTCTCCGGTCGTGTTGCGTTGAATTATGCCGTAAAAAAAAAAAAACGCCCCAAAAAAAAAAAAAGGGGCGGCTTTTTTTTTTAAAAAAGAGTGGTTTTATTTTAATAAAAATTTTTAAATAAATcaacaaag                                                                        >  1:1570762/1‑138 (MQ=255)
                                                                        ttttCTCCGGTCGTTTTCCATTAATTTGACCCGTAAAAAAAAGAAACCTCCCAATAAAAAAAATACCGTGCGGCTTTATTTTTTAAAAAGGAGGCGTTATATTTGTATAATAGTTTTAAAATAAACAAGCAAATGTTCgg                                                                  >  2:2147686/1‑140 (MQ=255)
                                                                                  tCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGa                                                        <  2:946142/140‑1 (MQ=255)
                                                                                                 ttCTCCCGTAAAATAAAGAAAGCTCCCAATACAACACATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCt                                         <  1:3189183/140‑1 (MQ=255)
                                                                                                   ctcCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTgg                                       <  2:3109528/140‑1 (MQ=255)
                                                                                                         tAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCAtttt                                 >  2:3688411/1‑140 (MQ=255)
                                                                                                           aaaTAAAGAAAGCTCCCAATACAACAACTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                               <  2:2103977/140‑1 (MQ=255)
                                                                                                           aaaTAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAg                               <  1:1659899/140‑1 (MQ=255)
                                                                                                             aTAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGtt                             <  2:343591/140‑1 (MQ=255)
                                                                                                                aagaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTATTGGTGGGATTTTAGTTGCg                          >  2:3871444/1‑140 (MQ=255)
                                                                                                                 agaaagCTCCCACTACACCAACTACGGTGCGGATTTATTATTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGt                         <  1:2435564/140‑1 (MQ=255)
                                                                                                                  gaaagCTCCCACTACAACAACGACGGTGCGGATTTATTTTTTAAACAGGAGTCGTTATATTTGAATAATAGATTTACAATAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                        <  1:1998441/140‑1 (MQ=255)
                                                                                                                  gaaagCTCCCAATACAACACATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                        <  1:3513333/140‑1 (MQ=255)
                                                                                                                  gaaagCTCCCAATACAACAACTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                        <  1:1845153/140‑1 (MQ=255)
                                                                                                                  gaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                        <  1:2593308/140‑1 (MQ=255)
                                                                                                                  gaaagCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGtt                        <  2:1714847/140‑1 (MQ=255)
                                                                                                                       cTCCCAACACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCAt                   >  1:4357116/1‑140 (MQ=255)
                                                                                                                        tCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTATCAtt                  >  2:721534/1‑140 (MQ=255)
                                                                                                                        tCCCAATACAACAAAGACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGATTTACATTAAAGCAGCAATGGTTCGGGAAGATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCAtt                  <  2:2286316/140‑1 (MQ=255)
                                                                                                                         cccAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTa                 <  1:4174373/140‑1 (MQ=255)
                                                                                                                                caacacATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTg          <  1:969999/140‑1 (MQ=255)
                                                                                                                                caacaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTg          <  2:3802310/140‑1 (MQ=255)
                                                                                                                                caacaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTg          <  1:2227189/140‑1 (MQ=255)
                                                                                                                                caacaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTg          <  1:1258698/140‑1 (MQ=255)
                                                                                                                                 aacaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGa         >  2:3622146/1‑140 (MQ=255)
                                                                                                                                  acaaATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAATCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGGATTTTAGTTGCGTTAGCATTAATACCTGaa        >  1:2354526/1‑140 (MQ=255)
                                                                                                                                      aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTAGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTTCGTTAGCATTAATACCTGAAGCaa    >  2:1598240/1‑140 (MQ=255)
                                                                                                                                      aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAAGGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATGTTAGTTGCGTTAGCATTAATACCTGAAGCaa    >  1:274115/1‑140 (MQ=255)
                                                                                                                                      aTACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTGATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCaa    >  1:538925/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                            
GATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCATTTTAGTTGCGTTAGCATTAATACCTGAAGCAATT  >  NZ_CP034943/176925‑177200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: