Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,730,0930GC28.0% 228.2 / 24.8 136E172Q (GAA→CAA) rodZcell shape determination protein RodZ
Reads supporting (aligned to +/- strand):  ref base G (44/51);  new base C (0/37);  total (45/91)
Fisher's exact test for biased strand distribution p-value = 1.53e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 153 total aligned reads displayed.

CGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAC  >  NZ_CP034943/1729954‑1730225
                                                                                                                                           |                                                                                                                                    
cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAATAGGCAGACACAAAAAAATAAACGAAAGAAAAAGAATATAAAAaag                                                                                                                                      >  1:2545026/1‑140 (MQ=255)
cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                                      >  2:3523577/1‑140 (MQ=255)
cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                                      >  2:1936077/1‑140 (MQ=255)
cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                                      >  1:2546346/1‑140 (MQ=255)
ataaTCTTAAAAATCAATTACGCAAATTTAAATAAAGTACGAAATCCAAAAATTTTCAACGCTAAAAGATAATCAAAGCAACAGCTCTTAAAAAGAGGCATACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                                      <  2:2033923/137‑1 (MQ=255)
 gcctcggAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAACTCCCAAAAGATTCAACGCTGAAAGTGACTCACAGCAACAGCACTGAAAAAGAGGCAGACCCTAAACATCAAACGACCGAAAAAGAAGAGAAAAaaga                                                                                                                                     >  1:1916923/7‑140 (MQ=255)
 gCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAACGAACAATAAGATAAAAaaga                                                                                                                                     >  2:2300504/1‑140 (MQ=255)
 gCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGAACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaaga                                                                                                                                     >  2:1264523/1‑140 (MQ=255)
  cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                                    >  2:178627/1‑140 (MQ=255)
  cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                                    >  2:1799011/1‑140 (MQ=255)
  cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                                    >  2:178671/1‑140 (MQ=255)
  cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAAAGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCATACACTAAAAAAGAAACGAAAGAAAAAGAAGATAaaaaaaaa                                                                                                                                    >  2:1079274/1‑140 (MQ=255)
               aaTTACGCAAAGTGAAAGCAAGTACTAAATCAAAAAATATTCAACGATAAAAGATAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACGAAACAAAAACAAGATAAAAAAGAAGAAAGCGACAGTg                                                                                                                       <  2:3074429/140‑1 (MQ=255)
               aaTTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGAGACAGTg                                                                                                                       >  1:2482857/1‑140 (MQ=255)
                  tACGCAAAGTGAAAGCAAGTACTAAATCCCAAAAGATTAAACTCTAAAAGATAATCAAACCAACAGATCTTAAAAAGAGGCAGACACTAAAAAATAAACCAAACGAAAAGAAGATAAAAAAGAAGAACGCGACAGTGATa                                                                                                                    <  2:450832/140‑1 (MQ=255)
                       aaaGTGAAAGCAATTACGAAATCCCAAAATATTCAACGCTAAAAGAGAATCAAACCAACAGATCTCAAAAAGAGGCAGACACTAAAAAAAAAACTAAAGAAAAAGAAGATAAAAAACAAGACAGCGACAGTGATAAACTg                                                                                                               <  2:1837285/140‑1 (MQ=255)
                       aaaGTGAAAGAAAGTACGAAATCCCAAAAGATTAAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCATACACTAAAAAAGAAACGAAACAAAAAGACGATAAAAAAGACCAAAGCGACAGTGATAAACTg                                                                                                               <  2:491583/140‑1 (MQ=255)
                       aaaGTCAAAGCAAGAACGAAATCCAAAAACATACAACGCTAAACCACAAAAAAAGCAACAGCTCTCAAAAAGAGGCAGACACTAAAAAAGAAACCAAACAAAAACAAGATAAAAAACAACAAAGCGACAGTGATAAACTg                                                                                                               <  2:2546346/140‑1 (MQ=255)
                            taaaGCAATTACGAAATCCCAAAAGATACAACGCTAAAAGAGAATCAAAGAAACAGCTCTGAAAAAGAGGCACACACTAAAAAATAAACCAAAGAAAAAGAAGATAAAAACCACCAAAGCGACAGTGATAAACTGGAGAt                                                                                                          <  2:86626/139‑1 (MQ=255)
                               aGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCCGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTaa                                                                                                       >  2:1979232/1‑140 (MQ=255)
                                   aGTACGAAATCCCAAAAGATTCAACGCTCAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                                   >  2:4146716/1‑140 (MQ=255)
                                   aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGACGATCAAAAAGAAGAAAGCGACAGTGATAGACTGGAGATTAAAGCa                                                                                                   >  1:2749319/1‑140 (MQ=255)
                                   aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACGGGAGATTAAAGCa                                                                                                   >  2:3770645/1‑140 (MQ=255)
                                     taacaaaaCCCAAAAGATTCAACGAAAAAATATAAAAAAAGCAACAGCTCTTAAAAAGAGCCACACACTAAAAAAAAAACCAAATAAAAAGACCATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 <  1:2788919/136‑1 (MQ=255)
                                     taacaaaaCCAAAAATATAAAACGCTAAAAAAAAAACAAACAACCAGCTCTAAAAAAGACGCACAAACTAAAAAACAAACCAAAAAAAACCAAGAAAAAAACCACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 <  1:481164/136‑1 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGTAGTAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  2:863453/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACCGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  1:895980/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  1:2604069/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGAGAAACTGGAGATTAAAGCAAc                                                                                                 >  2:2807162/1‑140 (MQ=255)
                                     tACAAAATCCAAAAATATAAAACTAAAAAACAAAAAAAAAGAAACAGCTCTCAAAAAGAGCAACACAAAAAAAAACAACCCAAACAAAAACAAAATAAAACACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 <  1:70708/140‑1 (MQ=255)
                                        gAAATCCCAAAAGATTCAACGCTAAAATAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTgg                                                                                              <  2:3398044/140‑1 (MQ=255)
                                        gAAATCCAAAAATATTAAACGCTAAAAGAGAATAAAAGCAACAGCTATGAAAAAGAGGCAGACACTAAAAAATAAACTAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTgg                                                                                              <  2:2882976/140‑1 (MQ=255)
                                        gAAATCAAAAAAGATTCAACGATAAAATATAATAAAAGCAAACCCACTGAAAAAGAGGAAGACAATAAAAAACAAACTAAAGAAAAAGAACATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAACGCAACTgg                                                                                              <  2:1916923/140‑1 (MQ=255)
                                          aaTCCCAAAAGATTCAACGCTAAAATAGAATCAAAGCAACAGCTCTGAAAACGAGGCACACACTAAAAAAGAAACGAAAGAAAAACACGATAAAAACGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCa                                                                                            <  2:2189782/140‑1 (MQ=255)
                                          aaTCCCAAAAGATTCAACGCTAAAAGAGAATCAATGCAACAGATCTTAAAACGAGGCAGACACTAAAAAAGAAACGAAAGAAAATGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCa                                                                                            <  2:1167380/140‑1 (MQ=255)
                                          aaTCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCACCTGGCa                                                                                            >  2:392540/1‑140 (MQ=255)
                                            tCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACt                                                                                          >  1:4361903/1‑140 (MQ=255)
                                             cccAAAAGATTAAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTg                                                                                         <  2:4451625/140‑1 (MQ=255)
                                              ccAAAATATCCACCGATAAAATATAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                                        <  2:4665248/140‑1 (MQ=255)
                                              acAAAAGATTCAACGCTAAAACATAATCAAAGCAAAAGCTATTAAAAACATGAATAAACTAAAAAATAAACGAAAGAAAAACAATATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                                        <  2:312555/139‑1 (MQ=255)
                                               cAAAATATACAACGCTAAAAGACAATCAAAGCAACAGCTCTTAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                       <  2:1199819/140‑1 (MQ=255)
                                               aaaaaTATTAAACGCTAAAAGACAATAAAAGCACCAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACGAAAGAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                       <  2:24797/139‑1 (MQ=255)
                                                 aaaGATTCAACGATAAAATATAATCAAACCAACAGATCTTAAAAAGAGGCACACAATAAAAAAGAAACGAAACAAAAAGAACATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAgg                                                                                     <  2:4226669/140‑1 (MQ=255)
                                                  aataTTCAACTATAAAAGAGAATCAAAGAAACACATATGAAAAAGAGGAAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                                    <  2:2182058/137‑1 (MQ=255)
                                                   ataTTCAACTATAAAAGACAATAAAACAAACAGTTTTTAAAACCCGCCACAAAATAAAAAATAAACTAAACAAAAACACTATAAAAAAGAAAAACGCGACGGTGATAAACTGGATATTAATGCAACTGGCACTGAAGGGt                                                                                   <  2:289729/138‑1 (MQ=255)
                                                       tCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                               >  1:3008650/1‑140 (MQ=255)
                                                         aacgaaaaaaCAAAATCAAAGCAACAGCTCTCAAAAAGAGGCACACAAAAAAAAACAAACGAAACAAAAACAACATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTaa                                                                             <  1:1937325/134‑1 (MQ=255)
                                                          ccccTAAAACATAATCAAAGCAACAGCTCTAAAAAAGAGGAACACACTAAAAAACAAACCAAAGAAAAAGAAGATAAAAAAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  1:3276562/137‑1 (MQ=255)
                                                          acgaaaaaaGATAAACAAAGAAACCTCTATCAAAAAGCGCCACACACAAAAAAACAAACCAAACAAAAACACGAGAAAAAAGAAGACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  1:1427281/135‑1 (MQ=255)
                                                          acgaaaaaaGACAATAAACCAAACCGCTCTCAAAAAGATCCACACAAAAAAAAACAAACCAACCAAAAACACGAAAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  1:1618320/135‑1 (MQ=255)
                                                          acgaaaaaaGACAATAAAAGCAACAGCTCTTAAAAAGAGGCATACACTAAAAAACAAACCAAACAAAAACAAGAAAAAAAACAACAACGCGACAGTGATAACCTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  1:1128723/135‑1 (MQ=255)
                                                          aCGCTAAAAGAGACACAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAACAGACACGAAAGAAAAAGATGATAAAAAAGAAGAAGGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  1:4247209/140‑1 (MQ=255)
                                                          aCGCTAAAAGAGAATCAAATCAACAGCTCTTAAAAAGAGGAAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCACCTGGCACTGAAGGGTCATTAAc                                                                            <  2:2297452/140‑1 (MQ=255)
                                                           cGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACg                                                                           >  2:771169/1‑140 (MQ=255)
                                                           cGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACg                                                                           >  2:912353/1‑140 (MQ=255)
                                                            caaaaacGAGAAACAAATCAACAGCTCTTAAAAACAGGAACACACAAAAAAAAAAACAAAACAAAACTAACATAAAAAACACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGa                                                                          <  1:2240572/137‑1 (MQ=255)
                                                                    ataaTCAAAGCAACAGCTCTTAAAAAGAGGCAGACACTAAAAAACAAACGAAAGAAAAAGACGATAAAAAAGACGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                  <  2:457202/138‑1 (MQ=255)
                                                                    ataaTAAAAGAAACCGCTCTTAAAAAGAGGCAGACACAAAAAAACAAACCAAACAAAAACACCAAAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                  <  1:2111550/138‑1 (MQ=255)
                                                                    acaaTAAAACCAACAGCTCTCAAAAATACGCACACACAAAAAAACAAACCAAACAAAAACAACATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                  <  1:1475081/138‑1 (MQ=255)
                                                                      aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                >  1:1209287/1‑140 (MQ=255)
                                                                      aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                >  1:3866573/1‑140 (MQ=255)
                                                                      aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                >  1:269387/1‑140 (MQ=255)
                                                                         cAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                             <  2:2604069/140‑1 (MQ=255)
                                                                                   gCTCTGAAAAAGAGCCACACACTAAAAAACAAACGAAACAAAAAGAAGATAAAAAAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTgag                                                   <  1:4609915/140‑1 (MQ=255)
                                                                                    ctctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATCAAAAAGAAGAAAGCGACAGTGCTCAAGTGGAGATGAAAGCAACTGGCCCGGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTgcga                                                  >  1:3939131/1‑140 (MQ=255)
                                                                                       tGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAAt                                               >  1:2846809/1‑140 (MQ=255)
                                                                                              gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                        >  2:505155/1‑140 (MQ=255)
                                                                                              gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                        >  2:635007/1‑140 (MQ=255)
                                                                                                 gCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCgc                                     >  1:2923458/1‑139 (MQ=255)
                                                                                                    caaacTAAAAAATAAACGAAACAAAAACAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACt                                  <  2:1494431/137‑1 (MQ=255)
                                                                                                      cacTACAAACCAAACGAACGAACACCACGACAAAAAAGAATAAAGCGACAGTGATAAACTGGAGATGAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAAGTCGAGCTCGAACTGa                                <  2:2749319/140‑1 (MQ=255)
                                                                                                      cacTAAAAAATAAACGAAACAAAACGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                <  2:513397/140‑1 (MQ=255)
                                                                                                      aacaaaaaaaCAAACCAAATAAAAATAACATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                <  1:4146716/136‑1 (MQ=255)
                                                                                                      aacTAAAAAACAAACCAAACAAAACAAACAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                <  1:4125402/139‑1 (MQ=255)
                                                                                                      aacTAAAAAACAAACCAAAAAAAAACAACATAAAAAACAAGACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                <  1:3770645/139‑1 (MQ=255)
                                                                                                      aaatAAAAAATAAACTAAATAAAAACACTATAAAAAACAATAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                <  1:1993925/137‑1 (MQ=255)
                                                                                                         tAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAgg                             >  1:934007/1‑140 (MQ=255)
                                                                                                         tAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAgg                             >  1:4054456/1‑140 (MQ=255)
                                                                                                         tAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAgg                             >  1:733571/1‑140 (MQ=255)
                                                                                                          aaaaaaGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGc                            >  1:2579616/1‑140 (MQ=255)
                                                                                                          aaaaaaGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGc                            >  1:3600011/1‑140 (MQ=255)
                                                                                                           aaaaaaaaACAAAAGAAAAATAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCa                           <  1:4469309/140‑1 (MQ=255)
                                                                                                           aaaaaTAAACTAAAGAAAAATATCATAAAAAATAAGAAAGCGACAGTGATAAACTGGAGATTAAATCAACTTGCACTGAAGTGTCATTAACGACTTATGAAGTGTCCGGCGCTGATAAATTCGAGCTCGAACTGAAGGCa                           <  2:1262707/140‑1 (MQ=255)
                                                                                                           aaaaaGAACCGAAAGAAAAATAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCa                           <  2:2482857/140‑1 (MQ=255)
                                                                                                           aaaaaCAAACAAAACAAAAACAACAAAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCa                           <  1:439748/140‑1 (MQ=255)
                                                                                                              aataaaCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCa                        <  2:48252/137‑1 (MQ=255)
                                                                                                              aaGAAACCAAATAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCa                        <  2:72390/140‑1 (MQ=255)
                                                                                                                taaaCTAAATAAAAATAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                      <  2:3008650/139‑1 (MQ=255)
                                                                                                                taaaCGAAATAAAATTAACATAAAAACCATGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCAGTGTAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                      <  2:3887480/139‑1 (MQ=255)
                                                                                                                cacaCGAACAAAAAACAACACAAAAACCAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                      <  1:1554343/137‑1 (MQ=255)
                                                                                                                caaaCTAAAGAAAAAGAAGATAAAAAAGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                      <  2:1405028/139‑1 (MQ=255)
                                                                                                                caaaCTAAAGAAAAACAACCTAACACAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                      <  2:740119/139‑1 (MQ=255)
                                                                                                                caaaCCAAAAAAAAACAACATAAAAACCAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                      <  2:1525957/139‑1 (MQ=255)
                                                                                                                 aaaCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGAc                     >  2:4090290/1‑140 (MQ=255)
                                                                                                                  aaCGAAACAAAAAGAAGATAAAAACCACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACa                    <  2:269387/140‑1 (MQ=255)
                                                                                                                  aaCCAAACAAAAAGAACATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACa                    <  2:1209287/140‑1 (MQ=255)
                                                                                                                   aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg                   >  2:3756199/1‑140 (MQ=255)
                                                                                                                   aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg                   >  1:678232/1‑140 (MQ=255)
                                                                                                                   aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg                   >  1:803174/1‑140 (MQ=255)
                                                                                                                           aaaagaaGATAAAACAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGa           <  2:3505906/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                    
CGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAC  >  NZ_CP034943/1729954‑1730225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑