Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,982,2360TC40.0% ‑6.0 / 14.7 15noncoding (284/1550 nt)EO946_RS1543516S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (3/6);  new base C (1/5);  total (4/11)
Fisher's exact test for biased strand distribution p-value = 6.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.41e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGTCAGACTTTCGTCCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTATGTTTGAACCATGCGGTTCAAACAAGCATCCGG  >  NZ_CP034943/2982123‑2982348
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cgacAGACGTTCGTCCATGGCGGAAGAGTCCCGACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCACGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCaa                                                                                        <  2:2653607/137‑1 (MQ=1)
          tcctCCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATg                                                                              <  2:4245506/137‑1 (MQ=1)
                              cccaCTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGTGAGCCGTTACCCCACCTACTAGCTAAt                                                                               >  1:3385783/4‑119 (MQ=0)
                              cccaCTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGTGAGCCGTTACCCCACCTACTAGCTAAt                                                                               <  2:3385783/116‑1 (MQ=0)
                                 acggctgcCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCAGTAAGTg                                                       <  2:3703482/137‑1 (MQ=0)
                                 accgctgcCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATGACCTCACCAACTAGCTAAGGCGCCGCGGGTCCATCTGTAAGTg                                                       <  2:1595988/137‑1 (MQ=0)
                                             cggaGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGcc                                           <  1:2799309/137‑1 (MQ=1)
                                                   aggcTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCttt                                     <  1:3903927/137‑1 (MQ=1)
                                                        ggcccGTGTCTCACTCCCAGTGTGGCCGATCACCCACTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCCCCAACGAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTATGt                                <  2:3821452/137‑1 (MQ=1)
                                                              tggctcAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTACTGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCCCCTTTTATTTTTGAAc                          >  2:2313531/4‑140 (MQ=0)
                                                                                gactataaCCCTCTCAGATCTGCTAAAAATCCTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTATGTTTGAACCATGCGGTTCAAACAAGc        >  2:964681/8‑140 (MQ=1)
                                                                                   gagcACCCTCTCAGGTCGGCTCCGCATCGGTGCCTTGGTGAGCCATTACCTCACCAACGAGCTACCGCGCCGCGGGTCCCTCTGTACGTGGTCGCCGAAGCCACCATGGATGTTGCAACCCTGGGGGTCAATCAAGCATc     <  2:3602314/137‑1 (MQ=0)
                                                                                   gaccACCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTATGTTTGAACCATGCGGTTCAAACAAGCATc     <  2:2862445/137‑1 (MQ=1)
                                                                                    atgaCCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTGAGCCAGTACCTCGCCAACTAGCTAATGCGCCGCTGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTATGTTTGAACCATGCGTTTCAAACAAGCATcc    <  2:3703567/137‑1 (MQ=1)
                                                                                      aacactatcAGGTCGGCTACGCATCGTTGAATTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTATGTTTGAACCATGCGGTTCAAACAAGCATCCgg  >  1:596562/8‑140 (MQ=0)
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CGTCAGACTTTCGTCCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTTGCCTTGGTGAGCCATTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTATGTTTGAACCATGCGGTTCAAACAAGCATCCGG  >  NZ_CP034943/2982123‑2982348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: