Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,270,0640GC24.8% 233.8 / 14.1 114L39V (CTA→GTA) prsWglutamic‑type intramembrane protease PrsW
Reads supporting (aligned to +/- strand):  ref base G (45/37);  new base C (1/26);  total (46/68)
Fisher's exact test for biased strand distribution p-value = 7.94e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 122 total aligned reads displayed.

ACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTTATATTC  >  NZ_CP034943/2269925‑2270203
                                                                                                                                           |                                                                                                                                           
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGACATACAAGAACAACCCCTAg                                                                                                                                             >  2:2071191/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  1:1144122/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  2:2138823/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  1:607136/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  1:730140/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                             >  2:1528805/1‑140 (MQ=255)
       taaTAAAACATTTTAATGATTGCTCCAAAAACCCTGAAGATAAAAAATAAACGAAAAAGCTTCCGCCTCCCTAATTCTCTTTTTCAAGCACTTACTGTATAAACATTGTGGTAAATACAAGAACAACCCCTATAAAAAAc                                                                                                                                      <  2:2523165/139‑1 (MQ=255)
          tAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGAc                                                                                                                                   >  2:10347/1‑140 (MQ=255)
          tAAAACATTTTATTGATTCCTCAAAAAACCATGAACATAAAAAACAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTAAAGAACGTACTGTATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGAc                                                                                                                                   <  2:730140/140‑1 (MQ=255)
            aaCCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACACGAACAACCCCTAGAAAAAACGACCg                                                                                                                                 >  1:463942/1‑140 (MQ=255)
            aaCCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTACAAAAAACGACCg                                                                                                                                 >  2:1161156/1‑140 (MQ=255)
             aCCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGa                                                                                                                                >  1:359191/1‑140 (MQ=255)
                tttttAATTATTCCACCAAAACCCCTTAACAAAAAAAAAAAACCAAAACGCTTCCCCCTCCCAAATTCTCTTTTTCAAGCACGTACTGTATAAACTTGGTGGTAAATACACCAACAACCCATACAAAAAACGACCGAAGc                                                                                                                             <  1:399686/139‑1 (MQ=255)
                cttttAATGAGTCCTCCAAACCCCCTGAAGATAAACAAGAAACCAAAAACCTTCCGCCCCCCAAATTCAATTTTTCAAGCCCGTACTGGATAAACTTTGTGGGAAATACAAGAACAACCCCCACAAAAAACGACCGAAGc                                                                                                                             <  1:2666175/139‑1 (MQ=255)
                aTTTTATTGATTCCACAAAAAACCATCAATATAAAAAACAAACCAAAACGCTTCCGCCTCCCACATTCTCTTTTTAAAGCAGGTAGTGCATAAACTTGGTGGTAAAAACACGAACACCCCCTACAAAAAACGACCGAAGc                                                                                                                             <  1:1533071/140‑1 (MQ=255)
                aTTTTAATGATTCCTCAAAAAACCCTTAATTTAAAAAATAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGTATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                             <  2:1676593/140‑1 (MQ=255)
                aTTTTAATGATTCCACAAAAACCCCTCAATATAAAAAACAAACCAAAACGCTCCCGCCACCCAATTTCTCTTTTTCAACCCCGTACTGGATAAACATTGTGTGAAATCCAACACCACCCCCTACAAAACACGACCGAAGc                                                                                                                             <  1:1153208/140‑1 (MQ=255)
                 ttttATTGTTTCCTCCAAAAACCCTTAATATAAAAAAGAAACTAAAAAGCTTCCGCCTCCCAATTTATATTTTTCAAGCACGTACTGTATAAACTTTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCa                                                                                                                            <  2:1212805/140‑1 (MQ=255)
                 ttttAATTTTTCCTCAAAACACCCTGAAGATAAAAAATAAAGTAAACAGTTTCCGCCTACAAAATTATGTTTTTCAATCAGGTAGTGGATAATCATTGTGTGAAATACAAGAAAACCCCGTAGAAAAAACGACCGAAGCa                                                                                                                            <  2:1229394/140‑1 (MQ=255)
                 ttttAATGATTCATCAAAAAACCCTGAAGATAAAAAATAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCa                                                                                                                            <  2:1596164/140‑1 (MQ=255)
                  tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTAGAAAAAACGACCGAAGCAc                                                                                                                           >  1:2302357/1‑140 (MQ=255)
                  tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                                           >  1:1536054/1‑140 (MQ=255)
                  tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                                           >  1:1951049/1‑140 (MQ=255)
                   ttACTGATTCCACCAAAAACCCTCACCATAAAAAAAAAACCAAAACGCTTCCGCCTCCCACTTTCTCTTTTTCAAGCACGTACTGTATAAACATTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCAcc                                                                                                                          <  1:339331/140‑1 (MQ=255)
                   ttAATGATTCCTCCAAAAACCCTGAAGATAAAAAATAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAcc                                                                                                                          <  2:1924287/140‑1 (MQ=255)
                   ttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAcc                                                                                                                          <  2:1536054/140‑1 (MQ=255)
                   ttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAcc                                                                                                                          <  2:1925152/140‑1 (MQ=255)
                     aaTTATACCTCAAAAAACCCTTAATAAAAAAAACAAACCAAAAAGCTTCCGCCCCCAACTTTATATTTTTCAAGCACGTACTGTATAAACATTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCACCat                                                                                                                        <  1:2138823/140‑1 (MQ=255)
                      aTGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCata                                                                                                                       <  2:570839/140‑1 (MQ=255)
                         agacatccAAAAACCCTTAATATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCAAATTCTCTTTTTAAAGCACGTACTGGATAAACATTGTGGGAAATAAAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGt                                                                                                                    <  2:2026016/135‑1 (MQ=255)
                          ttCCTCCAAAACCCCTTAAGATAAAAAAGAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTa                                                                                                                   <  2:925398/140‑1 (MQ=255)
                          ttCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACTTTGTGGGAAATACAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTa                                                                                                                   <  2:2525735/140‑1 (MQ=255)
                          ttCCTCCAAAAACCCTCAATATAAAAAACAAACCAAAAAGCTTCCGCCTCCCACATTCTATTTTTAAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTa                                                                                                                   <  2:1957020/140‑1 (MQ=255)
                           tcctccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTAc                                                                                                                  >  1:481583/1‑140 (MQ=255)
                                cAAAACCCCTGAACAAAAAAAAAAAACCAAAAAGCTTCCGCCTCCCAAATTATATTTTTCAAGCACGTACTGTAAAAACTTTGTGGGAAAAACAACAACAACCCCTACAAAACACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  1:1329441/140‑1 (MQ=255)
                                cAAAACCCCTCAACAAAAAAAACAAACACAAACGCTTCCCCCACCCACATTCTCTTTTTCAAGCACGTACTGCATAAAAATTGTGGCAAATACAACAACAACCCCCACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  1:551318/140‑1 (MQ=255)
                                cAAAAACCCTGAATATAAAACAGAAACTAAAAAGCTTCGGCCTCCCACATTCTATTTTTAAAGCACGTCCTGGATAACCCTTGGGGTAAAGACAATAACACCCCCGCGAAAAAACGCCCGAAGCACCATATGTACAGGtt                                                                                                             <  2:366083/140‑1 (MQ=255)
                                cAAAAACCCTCAATAAAAAAAACAAACGAAAACGCTTCCCCACCCCACATTCTTTTTTTCAACCACGTAGTTTATAAACATTGTGGTAAAAACAACAACACCCCCAAAAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  1:1915317/140‑1 (MQ=255)
                                aaaaacCCCTCAACATAAAAAACACCCCACAACGCTCCCGCCACCAAAATCTTCTTTTTCAACCCCGTACTGTATAAACTTTGTGGTAAATAAACGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  1:1680275/139‑1 (MQ=255)
                                aaaaacCCCTAAACAAAAAAAAGAAACCAAAACGCTACCGCCTCCCAAATTCTCTTTTTCACGCACGTCGGGGATAAACTTTGGGGTAAATACAAGAACAACCCCTACAAAACACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  1:1856277/139‑1 (MQ=255)
                                aaaaaaCCCTTAACATAAAAAACAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTTGATAAAAATTGTGGTAAATACAAGAAAAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                             <  2:1428447/139‑1 (MQ=255)
                                  aaaaCCATGAAGATAAAAAAGAAACTAAAAAGATTCCGCATCCCAATTTCTATTTTTAAAGCCAGTACTGGATAAACTTGGTGGTAAATACAACAACAACCCCTCCAAAAAACGACCGAAGCACCATATGTACAGGTTCa                                                                                                           <  1:2680709/140‑1 (MQ=255)
                                     ccccTGAATATAAAAAAGAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACGGGATAAACATTGTGGGAAATACAACAACAACCCCTCGAAAACACGACCGAAGCACCATATGTACAGGTTCATTa                                                                                                        <  2:463942/139‑1 (MQ=255)
                                     aCCCGGAATAAAAAAAATAAACGAAAACGCTCCCCCCCCCCACTTTCTATTTTTAAAGCACGTACTGTAAAAACATTGTGGTAAATACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTa                                                                                                        <  1:1125638/140‑1 (MQ=255)
                                         tGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCAt                                                                                                    >  1:2547009/1‑140 (MQ=255)
                                         tGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCAt                                                                                                    >  2:1592178/1‑140 (MQ=255)
                                                   aaaGAAACTAAAAAGCTTCCGGATCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGACCAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCttt                                                                                          <  2:118749/140‑1 (MQ=255)
                                                        aaCGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCGTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCAGACTGATCTTTtaaat                                                                                     >  1:748348/1‑140 (MQ=255)
                                                        aaCGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaat                                                                                     >  1:2314150/1‑140 (MQ=255)
                                                        aaCCAAAAAGCTACCGCCACCCACATTCTGTTTTTCAAGCAGCTAGTGTATAAAAATTTTGGTAAATACACCAACAACCCCTATAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaat                                                                                     <  1:585760/140‑1 (MQ=255)
                                                           gAAAAAGATTCCGCATCCCACATTCTCTTTTTCAAGAACGTACTGGATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                                  <  2:1457857/140‑1 (MQ=255)
                                                           caacaaGCGTCCGCCACCCACTTTCTATTTTAAAAGACCGTACTGGATAAAACTTGTTGTAAATAACAGAACAACCCCAACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                                  <  1:2183411/136‑1 (MQ=255)
                                                                     ccGCCTCCCACATTCTATTTTTCAAGCACGTCCTGTATAAACATTGTGGGAAATACAATAACAACCCATAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTaa                                                                        <  1:2431841/140‑1 (MQ=255)
                                                                                aTTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAAATTAACAGAGCGATGc                                                             >  2:2519851/1‑140 (MQ=255)
                                                                                 ttCTCTTTTACAAGCACTTACTGGATAAAATTTGTGGTAAATACAACAACAACCCCTAAAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGcc                                                            <  1:134918/140‑1 (MQ=255)
                                                                                 ttCTCGTTTTAAAGCACGTACTGGATAAACTTTGTGGGAAATACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGcc                                                            <  2:303222/140‑1 (MQ=255)
                                                                                    tctTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCggg                                                         >  2:2422285/1‑140 (MQ=255)
                                                                                      tttttCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                             >  1:2155349/1‑70 (MQ=255)
                                                                                      tttttCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAg                                                       >  2:1281306/1‑140 (MQ=255)
                                                                                       ttttCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGc                                                      <  2:359191/140‑1 (MQ=255)
                                                                                        tttCAAGTACATACTGGATAAACATGATGGGAAATACAAGAACAACCCCGAGAAAAAACGACCGTAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAATAACGCGATGCCGGGGGCa                                                     >  2:478446/1‑140 (MQ=255)
                                                                                          tCAAGCACTTACTGTATAAACATGGGGGTAAATACACCAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                                   <  1:518112/140‑1 (MQ=255)
                                                                                          acacgCACGTACTGCATCCAAATGGGTGTAAATACACGAACAACCCCGACAAAAACCGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTGTTAAATAAAAATAACTTACCAGCGCTATGCCGGGAGCAAt                                                   <  2:1232459/136‑1 (MQ=255)
                                                                                            aaGCACGTACTGGATAAACTTTTTGGTAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTc                                                 <  2:790644/140‑1 (MQ=255)
                                                                                              gCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCt                                               >  2:2145282/1‑140 (MQ=255)
                                                                                                 cGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCa                                            <  2:2547009/140‑1 (MQ=255)
                                                                                                   tACTGTATAAACATTGTGGTAAATACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCaga                                          <  1:1914810/140‑1 (MQ=255)
                                                                                                        gATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAgatga                                     >  2:328011/1‑140 (MQ=255)
                                                                                                          tAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGAtt                                   >  1:2705364/1‑140 (MQ=255)
                                                                                                             aCATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCACTTCCTGCAGAGATGATTGCa                                >  2:2485135/1‑140 (MQ=255)
                                                                                                             aCATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa                                >  2:2358795/1‑140 (MQ=255)
                                                                                                              cATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCaa                               <  1:2422285/140‑1 (MQ=255)
                                                                                                                  gtgGTAAACACAACAACACCCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATGATCATACTGATCTTTTAAATAAAAATAACTGAACAGCGCGAGGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                           <  1:127733/140‑1 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCCTATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                           >  1:117018/1‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                           >  1:1462768/1‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                           >  1:161038/1‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                           >  1:914728/1‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                           >  2:1292380/1‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                           >  1:929637/1‑140 (MQ=255)
                                                                                                                   tgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATc                          >  1:938562/1‑140 (MQ=255)
                                                                                                                       aaaTACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGc                      <  1:1292380/140‑1 (MQ=255)
                                                                                                                       aaaTAAAAGAACAACCCCTAGAAAAAACTACAGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGc                      <  2:1951049/140‑1 (MQ=255)
                                                                                                                         aTACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCaa                    >  1:49111/1‑140 (MQ=255)
                                                                                                                         aTACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTAAAGGTTCTTTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATGCCTGCAGAGATGATTGCAAACATCAGGCaa                    >  1:1190489/1‑140 (MQ=255)
                                                                                                                             aaGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACctc                >  1:272334/1‑140 (MQ=255)
                                                                                                                              aGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACctct               <  2:607136/140‑1 (MQ=255)
                                                                                                                              aGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACctct               <  1:1592178/140‑1 (MQ=255)
                                                                                                                                  caacCCCTAGAAAAAACGACCGAAGCACCATATGGACAGGTTCATTATCAGACTGATCTTTAAAATCAAAATAACTGAACAGCGCGATGCAGGGAGCAATTCCTGCAGAGATGATTCGAAACATCAGGCAACctcggtcc           >  1:1998143/1‑135 (MQ=255)
                                                                                                                                  caacCCCAACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTcc           <  1:275805/140‑1 (MQ=255)
                                                                                                                                     ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAGAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCttt        >  1:481970/1‑140 (MQ=255)
                                                                                                                                     ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGc                                                      <  1:1493868/94‑1 (MQ=255)
                                                                                                                                     ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACGTCTTTCCttt        >  1:1665954/1‑140 (MQ=255)
                                                                                                                                     ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCttt        >  2:563460/1‑140 (MQ=255)
                                                                                                                                     ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCttt        <  2:2314150/140‑1 (MQ=255)
                                                                                                                                     ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCt          <  2:255229/138‑1 (MQ=255)
                                                                                                                                     ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCt          >  1:255229/1‑138 (MQ=255)
                                                                                                                                     ccccGACAAAAAACGACCGAAGCACCATCTGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCttt        <  2:748348/140‑1 (MQ=255)
                                                                                                                                        cTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTtata     <  1:1281306/140‑1 (MQ=255)
                                                                                                                                        cTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTtata     <  2:2129580/140‑1 (MQ=255)
                                                                                                                                          aGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTTATAtt   >  1:873859/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTTATATTC  >  NZ_CP034943/2269925‑2270203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑