New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_CP034943	= 35429	629 (1.360)	6 (0.010) +AA	3/248	NT	0.97%	intergenic (+28/‑105)	csfB/EO946_RS00185	anti‑sigma‑G factor/5‑bromo‑4‑chloroindolyl phosphate hydrolysis family protein
	?	NZ_CP034943	35445 =	621 (1.340)					intergenic (+44/‑89)	csfB/EO946_RS00185	anti‑sigma‑G factor/5‑bromo‑4‑chloroindolyl phosphate hydrolysis family protein
Rejected: Frequency below/above cutoff threshold.

CGATCCTGACTATGCCTTTTACGTGAAAAAATTAAAGAGCATTCATACACCGCCATTATATTCTTAGACCTGAAAAGGTCTTTTTTTGTACTCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/35335‑35429 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCATAAAAAGAAGATGAAACTTGTTTAGGTATAGAACGTAGTAGATAATAATATTAAAACTGAGTATATACACAGGA  >  NZ_CP034943/35445‑35521                                                                                                ||                                                                              tGATCCTGACTATGAGTTTTACGTAAAAAAACTAAAGAGCATTCATACACCGCCATTATATTCATAGACCTGAAAAGGTCTTTTTTTGTACTCTTAATAATAAAAAGAAGATGAAACTTGTTTAAGGATTGAACGTAGTA                                    <  1:2185072/139‑1                      CGTAAAAAAACTAAAGAGCATTCATACACCGCCATTATATTCATAGACCTGAAAAGGTCTTTTTTTGTACTCTTAATAATAAAAAGAAGATGAAACTTGTTTAAGGATTGAACGTAGTAGATAATAATATTAAAACTGAG               <  1:6026484/140‑1                      CGTAAAAAAACTAAAGAGCATTCATACACCGCCATTATATTCATAGACCTGAAAAGGTCTTTTTTTGTACTCTTAATAATAAAAAGAAGATGAAACTTGTTTAAGGATTGAACGTAGTAGATAATAATATTAAAACTGAG               <  1:626832/140‑1                                   AAGAGCATTCATACACCGCCATTATATTCATAGACCTGAACAGGTCTTTTTTTGTACTCTTAATAATAAAAAGAAGATGAAACTTGTTTAAGGATTGAACGTAGTAGATAATAATATTAAAACTGAGTATAGACACAGGA  <  2:4437201/140‑1                                   AAGAGCATTCATACACCGCCATTATATTCATAGACCTGAAAAGGTCTTTTTTTGTACTCTTAATAATAAAAAGAAGATGAAACTTGTTTAAGGATTGAACGTAGTAGATAATAATATTAAAACTGAGTATAGACACAGGA  <  2:1737530/140‑1                                   AAGAGCATTCATACACCGCCATTATATTCATAGACCTGAAAAGGTCTTTTTTTGTACTCTTAATAATAAAAAGAAGATGAAACTTGTTTAAGGATTGAACGTAGTAGATAATAATATTAAAACTGAGTATAGACACAGGA  <  2:5175466/140‑1                                                                                                ||                                                                              CGATCCTGACTATGCCTTTTACGTGAAAAAATTAAAGAGCATTCATACACCGCCATTATATTCTTAGACCTGAAAAGGTCTTTTTTTGTACTCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/35335‑35429 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCATAAAAAGAAGATGAAACTTGTTTAGGTATAGAACGTAGTAGATAATAATATTAAAACTGAGTATATACACAGGA  >  NZ_CP034943/35445‑35521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.