New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 2924433541 (1.170)3 (0.010) 3/246 NT 0.56% coding (593/1107 nt) tbcS tetraprenyl‑beta‑curcumene synthase
?NZ_CP034943 2924437 = 536 (1.190)coding (589/1107 nt) tbcS tetraprenyl‑beta‑curcumene synthase
Rejected: Frequency below/above cutoff threshold.

AGACCTTGTATGTAAGGAAAATAACTATTGCGGATTTTTTTAGCGGTGTTTTCTGTGAAATCAGGCTGGAACGAATACGCAACAAGACAGAATATGCCTAACGTAGAGCCTGCACAGGCTGAAAATTCATACCATTCCATCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/2924292‑2924433
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tctCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAGTG  >  NZ_CP034943/2924437‑2924575
                                                                                                                                                                                                                                                                                         
AGACCTTGTATGTAAGGAAAATAACTATTGCGGATTTTTTTAGCGGTGTTTTCTGTGAAATCAGGCTGGAACGAATACGCAACAAGACAGAATATGCCTAACGTAGAGCCTGCACAGGCTGAAAATTCATACCATTCCAT                                                                                                                                               >  1:1550675/1‑140
AGACCTTGTATGTAAGGAAAATAACTATTGCGGATTTTTTTAGCGGTGTTTTCTGTGAAATCAGGCTGGAACGAATACGCAACAAGACAGAATATGCCTAACGTAGAGCCTGCACAGGCTGAAAATTCATACCATTCCAT                                                                                                                                               >  1:3616383/1‑140
AGACCTTGTATGTAAGGAAAATAACTATTGCGGATTTTTTTAGCGGTGTTTTCTGTGAAATCAGGCTGGAACGAATACGCAACAAGACAGAATATGCCTAACGTAGAGCCTGCACAGGCTGAAAATTCATACCATTCCAT                                                                                                                                               <  1:5975947/140‑1
                                             GTGCTTTCTGTGAAATCAGGCTGGAACGAATACGCAACCAGACAGAAAATGCCTAACGTTGAGCCTGCACAGGCTGAAAATTCATACCATTCCATCTCAGGCAGCTCTGATTCATATTGAGTAAACCACTTTTCAAGCCG                                                                                                  <  2:4906929/140‑1
                                                                                                                CACAGGCTGAAAATTCATACCATTCCATCTCAGGCAGCTCTGATTCATATTGAGTAAACCACTTTTCAAGCCGCGGAACACGCTCATGCTCAATAACGTGCTTATGCACCTGCAAATCGCAATAATAGCCG                                        <  1:2005836/131‑1
                                                                                                                CACAGGCTGAAAATTCATACCATTCCATCTCAGGCAGCTCTGATTCATATTGAGTAAACCACTTTTCAAGCCGCGGAACACGCTCATGCTCAATAACGTGCTTATGCACCTGCAAATCGCAATAATAGCCG                                        >  2:2005836/1‑131
                                                                                                                                          gTCTCAGGCACCTCTGATTCATACTGAGGAAACCCCTTCTCACGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTA     <  2:7210816/139‑1
                                                                                                                                           TCTCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCACTAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAG    >  2:5452888/1‑140
                                                                                                                                           TCTCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAG    >  2:825975/1‑140
                                                                                                                                           TCTCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAG    >  2:7011534/1‑140
                                                                                                                                           TCTCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAG    >  1:6556/1‑140
                                                                                                                                           TCTCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAG    >  1:6262395/1‑140
                                                                                                                                           TCTCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCA                                      <  2:2709024/106‑1
                                                                                                                                           TCTCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCA                                      <  2:3388942/106‑1
                                                                                                                                           TCTCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCA                                      >  1:3388942/1‑106
                                                                                                                                           TCTCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCA                                      >  1:2709024/1‑106
                                                                                                                                           TCTCAGGCAGCTCTGATTAATACTGAGTAAACCACTTCTCAAGCAGCGGGACACGCTCATCCTCAATGACGTCCTTATGTACCTGCAAAGAGCAATACGAGCCGCACAGTTCAAGCAGATAAGGTTTGATCCTTTCGTAG    >  2:7284000/1‑140
                                                                                                                                             TCAGGCAGCTCTGATTCATACTTAGTAAACAACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGTCGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATGATGTCGTAGTG  <  2:5134910/140‑1
                                                                                                                                             TCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAGTG  <  2:4252527/140‑1
                                                                                                                                             TCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAGTG  <  2:461486/140‑1
                                                                                                                                             TCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAGTG  <  2:461560/140‑1
                                                                                                                                             TCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAGTG  >  1:5788271/1‑140
                                                                                                                                             TCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAGTG  <  1:5522283/140‑1
                                                                                                                                             TCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAGTG  <  2:5644062/140‑1
                                                                                                                                             TCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAGTG  <  1:520500/140‑1
                                                                                                                                                                                                                                                                                         
AGACCTTGTATGTAAGGAAAATAACTATTGCGGATTTTTTTAGCGGTGTTTTCTGTGAAATCAGGCTGGAACGAATACGCAACAAGACAGAATATGCCTAACGTAGAGCCTGCACAGGCTGAAAATTCATACCATTCCATCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/2924292‑2924433
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tctCAGGCAGCTCTGATTCATACTGAGTAAACCACTTCTCAAGCCGCGGGACACGCTCATGCTCAATGACGTGCTTATGTACCTGCAAATCGCAATAATAGCCGCACAGTTCAAGCAGATAAGGTTTGATCATGTCGTAGTG  >  NZ_CP034943/2924437‑2924575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.