Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 17,872 A→C 13.5% K243N (AAA→AAC dacA → D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP03494317,8720AC13.5% 864.8 / 8.4 361K243N (AAA→AACdacAD‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base A (215/54);  new base C (2/57);  total (218/143)
Fisher's exact test for biased strand distribution p-value = 5.86e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 388 total aligned reads displayed.

CTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGC  >  NZ_CP034943/17733‑18010
                                                                                                                                           |                                                                                                                                          
cTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAAATTTATGCTGaaa                                                                                                                                            <  1:1732668/140‑1 (MQ=255)
    ggACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGc                                                                                                                                        <  2:228496/140‑1 (MQ=255)
    ggACAGGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGc                                                                                                                                        <  2:4167870/140‑1 (MQ=255)
        aTGGCAATTCTTGCGGATCACTTGCTTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATGGCGATAACGAAGTTCAGAGAAGGCAGAGCTGATGACATGGAGCTGCCGAACTGGAACTTTATGGTGCACGGGCgtgt                                                                                                                                    >  2:6429599/1‑140 (MQ=255)
          ggCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGATACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGCCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCa                                                                                                                                  >  1:1243425/1‑140 (MQ=255)
          ggCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCa                                                                                                                                  >  2:5975136/1‑140 (MQ=255)
          ggCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCa                                                                                                                                  >  1:3552188/1‑140 (MQ=255)
           gCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAg                                                                                                                                 <  1:6064805/140‑1 (MQ=255)
            cAATTCTTGCGGATCACTTGATTACTGACTCCCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                                                <  2:5466409/140‑1 (MQ=255)
            cAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                                                <  1:2311673/140‑1 (MQ=255)
            cAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                                                <  2:5832769/140‑1 (MQ=255)
            cAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                                                <  2:5114766/140‑1 (MQ=255)
            cAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                                                <  1:7375220/140‑1 (MQ=255)
               ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                                             >  1:6930577/1‑140 (MQ=255)
               ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                                             >  1:1509271/1‑140 (MQ=255)
               ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                                             >  1:769544/1‑140 (MQ=255)
               ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                                             >  1:3487010/1‑140 (MQ=255)
                    gCGGATCACTTGATTACTGACTACCCTTAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATaa                                                                                                                        >  2:4625095/1‑140 (MQ=255)
                    gCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATaa                                                                                                                        >  1:4073835/1‑140 (MQ=255)
                        aTCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATaaaaaa                                                                                                                    >  2:7352148/1‑140 (MQ=255)
                                  tACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAg                                                                                                          >  1:7535646/1‑140 (MQ=255)
                                  tACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAg                                                                                                          >  1:7180191/1‑140 (MQ=255)
                                  tACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTAGAAAAAAGCGACTGTAg                                                                                                          >  2:2156311/1‑140 (MQ=255)
                                  tACTGACTACCCTGAGATTCTGGAGACTTCCAGAATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGAGATGCCGAACTGGAACTTTATGCTGAAAGGGCGTGTCAGCGAGTATAAAAAAGCGCCTGTAg                                                                                                          >  1:2733672/1‑140 (MQ=255)
                                   actcactcCCCTTATATTCTGTAGACTTCATTTATCGTTAAAACGAAGTTCCTACAAGGAAAATTTTATTAAATGTCCTTGCAGAAGGGGAACTTTTTGCTGAACGGGTTTGTCAGCTATTATAAAAAAGCGACTGTAGa                                                                                                         <  2:3772719/140‑1 (MQ=255)
                                   aCTGACTACACTTATTTTGTGGACATTTCCTTTATCGCAAACACGATGTTCATAGAAGGCACCGATTTTTAATTGGACTTCCCGACCTGCACGTTTATGCTCACCGGGCTTGTCAGCGATTATAAAAAAGCGACTGTAGa                                                                                                         <  2:4907578/140‑1 (MQ=255)
                                   aCTCACTACCCTGATATGCGGCACACTTCCTGTATCTCTAAAACTAATTTCAGACAAGCCACAGATTATGAAATGGACATGCCTACGTTGAACTTTTCGCTGAACGGGCTTTTATGCGATTATAAAAAAGCGACTGTAGa                                                                                                         <  2:2854352/140‑1 (MQ=255)
                                       aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                                                >  2:7057317/1‑113 (MQ=255)
                                       aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                                     >  2:6249349/1‑140 (MQ=255)
                                       aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                                     >  1:5938260/1‑140 (MQ=255)
                                           cccTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGa                                                                                                 >  1:2934064/1‑140 (MQ=255)
                                              tGAGATGCTGGTGACTTCCAGTATCGCGAAAACGAAGTTCAGAGAAGGCCCAGATGATGCAATGGACATGCCGACCGGGAACTTTATGCTGAAAGGGCTTGGCAGCGAGGATACAAAAGCGACTGTAGACGGCTTGaaaa                                                                                              >  1:7042862/1‑140 (MQ=255)
                                               gttagtCTGGACACTTCAAGTAGCGCTAAACCGATGTTCAGAGAAGCCAAAGATTAGTAACTGGAAATGCCGAAATGCAACTTTATGCTCAAAGGGCTTGTCAGCGCGTTTAAAAAAGCGACTGTAGACGGATTGACAAc                                                                                             <  2:7207724/135‑1 (MQ=255)
                                                agaTACTTGAGACTTACAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATAGAAATGACGAACTGGAACTTTATGATGAAATGTCTTGTCAGCGAGTATAAAAAAGAGAATGTAGACGTATTGAAAACa                                                                                            >  2:6840195/1‑140 (MQ=255)
                                                  ctccgggAGACTTCCAGTATCGCCAAACGCAAATTCCCCCATGGCACCGATGATGAAAGGGACAGCCCGAACTGGATTTTTAGGCTGACGGGGCGTGTCAGCGATTATAAAAAAGCGACTGTAGACGGACTGAAAACAgg                                                                                          <  2:6044516/135‑1 (MQ=255)
                                                  aTTCTGTATACTTCAATTATCGATAAACCTAAGTTCAGAGACGGAACATATTATTAAATGGACATGCCTAACTGCAAGTTTTTGCTGAACGGGGTTGTCAGCTATTATAAAAAAGCGACTGTAGACGGATTGAAAACAgg                                                                                          <  2:3552188/140‑1 (MQ=255)
                                                   ttCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGt                                                                                         >  1:4603452/1‑140 (MQ=255)
                                                      tGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGc                                                                                                                                >  1:2449194/1‑98 (MQ=255)
                                                      tGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCt                                                                                      >  2:6776164/1‑140 (MQ=255)
                                                       gtagaCTTCAATTTTCGATAAAACGATTTTCACATAAGGAACATATTATGAAATGGAATTGCCGAACTGGAATTTTATGCTGAACGGGCTTGTCAGCGATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTa                                                                                     <  2:3307138/138‑1 (MQ=255)
                                                        gagaCTTCCAGTATCGCCAAAACGAAATTCAGAGAAGGCACCGATGATGAAATGGACATGCCGTACTGGAATTTTATGCTGAAGGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGACTGACAACCGGTTCTAc                                                                                    >  1:5396480/1‑140 (MQ=255)
                                                           aCTTCCAGTATCGCTAAACCTACTTTCAGAGAAGCCACATATGAGCAAATGGACATCCCCAACTGGAACTTTTTGCGCATGGGGTTTGTCAGCTAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGa                                                                                 <  2:2981485/140‑1 (MQ=255)
                                                             ttCCTGTAGCGCTAAAACTAAGTACCGAGACGGCACATTTTTTTAAATGGCCATGCCGACGTGCACGTTTATGCGGAACGGGCTGGCCCGCGATTATAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGAct                                                                               <  2:4545330/140‑1 (MQ=255)
                                                             ttCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGAct                                                                               >  1:4195312/1‑140 (MQ=255)
                                                             ttCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGAct                                                                               >  2:7536597/1‑140 (MQ=255)
                                                             ttCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGAct                                                                               >  1:1347567/1‑140 (MQ=255)
                                                                ctttATCGATAAAACGATTTTCACAGAAGGCACATTTTTTTAATTGCACATGCCGAACTGTAAATTTATGCTGAAAGGGCTTGACAGCGATTAAAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTg                                                                            <  2:4988993/137‑1 (MQ=255)
                                                                cAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTg                                                                            >  2:1618661/1‑140 (MQ=255)
                                                                       gCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTc                                                                     >  1:285842/1‑140 (MQ=255)
                                                                           aaacctaGTTCACAGAAGCCACAGATCATGAAATGCACATGCTGAACTGGAATTTTATGCTGAAAGGGCTTGCACGCGATAATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGc                                                                 <  2:5276970/134‑1 (MQ=255)
                                                                           aaaCTAATTTCAGATAAGGCACATATTATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGc                                                                 <  2:4073835/140‑1 (MQ=255)
                                                                           aaaCTAAGTTCATCCAAGGCACATATTATCAAAGGGACATGCCGAACTGGAACTTTAGGCTGAACGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGc                                                                 <  2:6210824/140‑1 (MQ=255)
                                                                           aaaCGAATTTCAGAGACGCCACAGATGATGAAATGGACAGGCCGAACTGCACCTTTATGCTGAACGGGCTTGTCAGCGAGTATAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGc                                                                 <  2:7594033/140‑1 (MQ=255)
                                                                           aaaCGAAGTTAACAGACGGCAAATATGATTAAATGCACATGCCCAACTGTAAATTTATGCTCAACGGGCTTGTCAGCTAGTATAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGc                                                                 <  1:5186359/140‑1 (MQ=255)
                                                                           aaaCCAAGTTACCAGAAGGCACAGATTATGAATGGGAAATGCCCACCTGCAATTTTATGCTGAATGGGGTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGc                                                                 <  2:139232/140‑1 (MQ=255)
                                                                                  gTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAg                                                          >  2:1689243/1‑140 (MQ=255)
                                                                                   ttCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAgg                                                         >  2:6109795/1‑140 (MQ=255)
                                                                                     cAGATACGGCACTGATTTTTAATTGTACTTGCCCACCTGGAATTTTATGCTGACCGGGGTTTTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGaa                                                       <  2:663886/140‑1 (MQ=255)
                                                                                        agaAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAg                                                    >  1:5255027/1‑140 (MQ=255)
                                                                                             gCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAACGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTAGTGAGTCTGCGGGCTCATGCTTTACAGGAACAGCTGaa                                               >  1:5647950/1‑140 (MQ=255)
                                                                                             gCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGCAACAGCTGaa                                               >  2:320329/1‑140 (MQ=255)
                                                                                                  ttttattaaaTGGACTTCCCTAAGTGCAATTTTAGGCGTACGGGGTTTGTCAGCGCTTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCaa                                          <  1:998035/133‑1 (MQ=255)
                                                                                                      atgaAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGa                                      >  2:2466735/1‑140 (MQ=255)
                                                                                                      atgaAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGa                                      >  1:6800290/1‑140 (MQ=255)
                                                                                                        gaAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAAt                                    >  1:2354377/1‑140 (MQ=255)
                                                                                                         aattgtaCATGCCTAACTTTAACTTTTTGCTCAACGGGTTTTTCCGCTATTAAAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                                   <  1:5854045/134‑1 (MQ=255)
                                                                                                         aattgcaCATGCCCAAGTGCAACTTTATCCTAAAAGGTCTTTGATCCCACTAAAAAAAAGCGACTGTAGACTGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                                   <  1:7590055/134‑1 (MQ=255)
                                                                                                         aatggcaCATGCCGAACTGGAAATTTATGCTCAACGGGTTTGTCTGCGATTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                                   <  1:6566209/134‑1 (MQ=255)
                                                                                                         aactGGACTTCCCTAAGTGGAACTTTATGATGAACGGGGTGGTCCGCGTTTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                                   <  1:6320298/137‑1 (MQ=255)
                                                                                                          agtgtaCATCCCGAAGTGTAAATTTCTCTTGAACGGGGTGGGCAGCTATTATAAAACAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGc                                  <  1:4435520/135‑1 (MQ=255)
                                                                                                          actGGACATGCCGAACTGGAACTTTATGCTGAACGGGCTTGTCAGCGATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGc                                  <  2:3404404/138‑1 (MQ=255)
                                                                                                          aaTGTACTTGCCGAACTGCAACTTATTGATGAATGGGGTTTACAGCTAGTAAACAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGc                                  <  2:1841379/140‑1 (MQ=255)
                                                                                                           ttGGACAGGCCGACGGGGAACTTTTGGGTCAATGGTGTTGTCACCGAGTTTAACAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg                                 <  2:1148733/139‑1 (MQ=255)
                                                                                                           aTGGACATGCCGACCTGTAAATTTATGCTGAAAGGGGTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg                                 <  2:445605/140‑1 (MQ=255)
                                                                                                           aTGGACATGCCGAACTTGAATTTTAGGCTTAATTTGTTTGTAAGCGATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg                                 <  2:2595195/140‑1 (MQ=255)
                                                                                                           aTGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg                                 >  2:2750070/1‑140 (MQ=255)
                                                                                                           aTGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCCAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg                                 >  2:6998461/1‑140 (MQ=255)
                                                                                                           aTGGAACTGCCGACCGGCAACTTTCTGGTTAAGGGGTTTGTCCGCTTGTATAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg                                 <  1:3793764/140‑1 (MQ=255)
                                                                                                           aTGCACATGCCCAACTGCAAGTTTATGCTCACTGGGCTTGTCCGCGCGTATAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg                                 <  2:2418368/140‑1 (MQ=255)
                                                                                                            tGGACTTGCCCAAGTGCAATTTTTTGCCCAATGGGCTTGGCCGCTATTATAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCgt                                <  1:4845713/140‑1 (MQ=255)
                                                                                                              gaactGCCGAACTGTAACTTTATGCTTAACGGGCTTGACAGCGATTAAAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCgtgt                              <  1:7068391/136‑1 (MQ=255)
                                                                                                              gACATGCCGACCTGGAACTTTATGCTTAACGGGCTTGTCAGCTATTATAAAAAAGCGACTGTAGGCGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCgtgt                              <  2:769544/140‑1 (MQ=255)
                                                                                                              gACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCgtgt                              >  2:7278428/1‑140 (MQ=255)
                                                                                                              gACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCgtgt                              >  2:1094586/1‑140 (MQ=255)
                                                                                                              gACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCgtgt                              >  2:1924079/1‑140 (MQ=255)
                                                                                                                   gCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGACCACAGGTTCTACTGACTCTGCGGGCTCATGCTGTACAGGACCAGCTGAACTCAACGGAATGCGTGttattc                         >  1:1028182/1‑139 (MQ=255)
                                                                                                                   gCCCAACTGGAAATTTATGCTGAACGGGCTTGCCCGCGATTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGttatta                         <  1:1013184/140‑1 (MQ=255)
                                                                                                                   cgtgAACTGTAATTTTTTGCTTAACGGGTTTTTATGGGTTTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTTttatta                         <  1:1620739/137‑1 (MQ=255)
                                                                                                                      caaCTGGAAATTTATGAGGCCCGGGCTGTTAAGCGAGAAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACtg                      <  1:2174538/139‑1 (MQ=255)
                                                                                                                             aaCTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTAtt               >  1:2552614/1‑140 (MQ=255)
                                                                                                                                  tATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACg          >  1:3178326/1‑140 (MQ=255)
                                                                                                                                   aTGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGc         >  1:4318832/1‑140 (MQ=255)
                                                                                                                                      cTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCaaa      >  2:2053197/1‑140 (MQ=255)
                                                                                                                                         aaaGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                                   >  1:7487934/1‑108 (MQ=255)
                                                                                                                                         aaaGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAgg   >  1:6425802/1‑140 (MQ=255)
                                                                                                                                         aaaGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAgg   >  1:6183770/1‑140 (MQ=255)
                                                                                                                                         aaaGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAgg   >  1:5495256/1‑140 (MQ=255)
                                                                                                                                         aaaGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAgg   >  2:943766/1‑140 (MQ=255)
                                                                                                                                          caGGGGTGGTCCGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGc  <  1:5099624/139‑1 (MQ=255)
                                                                                                                                          aaGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGc  >  1:674082/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CTAAGGACATGGCAATTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGC  >  NZ_CP034943/17733‑18010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: