Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 316,262 C→A 7.5% Q28K (CAA→AAA)  opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943316,2620CA 7.5% 1577.4 / 25.6 643Q28K (CAA→AAA) opuAAglycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Reads supporting (aligned to +/- strand):  ref base C (383/188);  new base A (3/68);  total (386/257)
Fisher's exact test for biased strand distribution p-value = 2.73e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 654 total aligned reads displayed.

GTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAC  >  NZ_CP034943/316123‑316401
                                                                                                                                           |                                                                                                                                           
gtgtgGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATAGGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTc                                                                                                                                             >  1:7227363/1‑140 (MQ=255)
   tgGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCaaa                                                                                                                                          >  2:7184965/1‑140 (MQ=255)
   tgGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCaaa                                                                                                                                          >  1:4939414/1‑140 (MQ=255)
   tgGAATGTTACATAAGTGCTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGAAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGTAAACAAACAAAGAAAGCAGTTCaaa                                                                                                                                          >  2:1552007/1‑140 (MQ=255)
       ctGTTACATAAGTGTTACGGTGATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATCAAAGTCGAGCAAGGATCTACAATTTTTGGGAAACAAACAAAGAAGGCAGTTCAAATGCt                                                                                                                                      >  1:2306002/2‑140 (MQ=255)
       aTGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAATAAAGCAGTTCAAATGCt                                                                                                                                      >  1:5622683/1‑140 (MQ=255)
                  gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTCTCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaa                                                                                                                           >  1:1647615/1‑140 (MQ=255)
                  gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaa                                                                                                                           >  1:3291580/1‑140 (MQ=255)
                   tgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaac                                                                                                                          >  2:4312924/1‑139 (MQ=255)
                     ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTATAGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                        >  1:7475281/1‑140 (MQ=255)
                       aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTAGCTAGAGTTTTTGGGAAACAAACACAGAAAGCAGTTCAAATGCTTGCCAACGGCAAGAcc                                                                                                                      >  2:7048916/1‑139 (MQ=255)
                       aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGACGAGGAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                      >  2:411157/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  2:3104871/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  1:5960769/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  2:132156/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTAGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  2:6492844/1‑140 (MQ=255)
                               aaGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAaga                                                                                                              <  1:2175586/140‑1 (MQ=255)
                               aaGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAaga                                                                                                              <  2:1629823/140‑1 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAATTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTACAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  2:4834521/1‑140 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAATCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  2:1386996/1‑140 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCTGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:2811600/1‑140 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:802429/1‑140 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  2:1784062/1‑140 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:109838/1‑140 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:3880359/1‑140 (MQ=255)
                                       tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACATACAAAGACAGCCGTTCAAATGCTTGCCAACGGCCTGAGACACAAAGAGATCCGGa                                                                                                      >  2:5227331/1‑140 (MQ=255)
                                       tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTc                                                                                                                                             <  1:4380150/101‑1 (MQ=255)
                                       tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTc                                                                                                                                             >  2:4380150/1‑101 (MQ=255)
                                              tGGAGGTAAAATATTTTTGTAGATGATAAACAAATTAAGATAAAAGTCTAGAAATTATCTAAAATTTTTGGCAAACAAACAAAGAAACCAGTTCAAATGCTTGCCAACGGAACCACAAAAAAAGAGATCCTGAAAGCGAc                                                                                               <  2:103886/140‑1 (MQ=255)
                                              gggAGGGAAAATATGAGTGTATATCAGAAACAAATTAATATAAAAGTCGAGAAAGTATCTAAAATTTTTGGTAAAAAAACAAATAAAGCATTTAAAATGCTTGCCAACGCAAAGAAAAAAAAAGAGATCCTGAAAGCGAc                                                                                               <  2:855905/139‑1 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAACGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:6579489/1‑140 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                        >  2:6334972/1‑112 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACACAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:4904946/1‑140 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:4620757/1‑140 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:7275020/1‑140 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:1231329/1‑140 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:3341499/1‑140 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:3156826/1‑140 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:6664880/1‑140 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgc                                                                                            >  1:2566601/1‑139 (MQ=255)
                                                 aGGGAAAATATGAGGGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGGATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTTCCCACGGAAAGACAAAAAAAGAGATCCTGACAGCGACCgg                                                                                            >  1:2984031/1‑140 (MQ=255)
                                                    caaaaTATTCGTCCAGATCACAACCCAATAAACAAAAAAGTCGAGAACGTATATAAAATTTTTGCGAAACACACAAACAAACCAGTAAAAAAGCTGGCCAACGCAAACACAAAAAAACAAATCCTGAAAGCGACCGGATc                                                                                         <  2:2649782/139‑1 (MQ=255)
                                                    caaaaTATGAGTGTAGATTAGAACACAATTAACATAAAAGTCCACAAAGTATCTAAAATTTTTGGGAAACAAACAAACAAAGCAGTTAAAATGCTTCCCAACGGAAACACAAAAAAAGAGAACCTGAAAGCGACCGGATc                                                                                         <  2:6417469/139‑1 (MQ=255)
                                                      aaaTATGAGTGTAGAGGAGAAACCAATTAAGATACAAGTCGAGAAAGTATCTAAAATTTTTGGGCAACAAACAAAGAAAGCAGTTCAATTGCTTGCCAACGGAAAGAGACTAAAAGAGATCCTGAAAGCGAGCGGCTCag                                                                                       >  1:1000992/1‑139 (MQ=255)
                                                              ttgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAATTATCTAAAATTTTTGGGAAACAAAAAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTggg                                                                               <  2:2991787/139‑1 (MQ=255)
                                                               tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  1:6134398/1‑140 (MQ=255)
                                                               tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  1:695045/1‑140 (MQ=255)
                                                               tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAACAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  2:6826635/1‑140 (MQ=255)
                                                                 tAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTa                                                                            >  2:1223978/1‑140 (MQ=255)
                                                                 tAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTa                                                                            >  2:2028089/1‑140 (MQ=255)
                                                                   gATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGACAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCCACCGTGGGGGTaaa                                                                          >  1:6488585/1‑140 (MQ=255)
                                                                    aTGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAAt                                                                         >  2:1966199/1‑140 (MQ=255)
                                                                        gaAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAg                                                                     >  1:3401884/1‑140 (MQ=255)
                                                                        caaaCAAATAAACATAAAAGTCTACAAAGTAACTAAAATTTTTGGGAAACAAACAAAGAAAGCCGTTCAAATCCTTGCCAACGCAAAGAAAAAAAAAGACACCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAg                                                                     <  2:2160924/139‑1 (MQ=255)
                                                                         aaaCCAATTACGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTGGCCAACGGACAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCACCCGTTGGGGTAAATCAAGc                                                                    >  2:6627569/1‑140 (MQ=255)
                                                                         aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACACACAAAGAAAGCAGTTCAAATGCTTGCCAACGGACAGAGAAAAAAAGAGATCCTGCACGAGACCGGATCAACCGTGGGGGTCACTCAGGc                                                                    >  1:6262918/1‑140 (MQ=255)
                                                                         aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  2:1130269/1‑140 (MQ=255)
                                                                         aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:4046886/1‑140 (MQ=255)
                                                                         aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:3621603/1‑140 (MQ=255)
                                                                         aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAACAAAGCAGTTCAAATGCTTGCCACCGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    <  2:7364279/140‑1 (MQ=255)
                                                                              aaTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtt                                                               >  1:194660/1‑140 (MQ=255)
                                                                                ttAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtttt                                                             >  1:759963/1‑140 (MQ=255)
                                                                                ttAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtttt                                                             >  1:5110405/1‑140 (MQ=255)
                                                                                 tactaTAAAAGCCGAGAACGTATAAAAATTGTTTGGCAAACAAACAAACAACGCCGTTCAAATGCGGCCAAACGCAAACACAAAAAAACAGACCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  1:6821723/136‑1 (MQ=255)
                                                                                 taacaaaaaaGTCGACACAGTATCTAAAATTTGGGGTAAACAAACAACGAAAGCAGTTCAACGGCTTGCCAACGCAAACACAAAAAAAGACAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  1:267921/134‑1 (MQ=255)
                                                                                 tAACATAAAAGTCTACAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  2:3439146/140‑1 (MQ=255)
                                                                                   ataTAAAAGTCGATAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAATTGGTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  2:7475281/138‑1 (MQ=255)
                                                                                   aGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  2:4702117/140‑1 (MQ=255)
                                                                                   aGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  2:2771920/140‑1 (MQ=255)
                                                                                   aGATAAAAGTCGAGAAAGAATCTAAAATTTTTGGCAAACAAACAACCAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  2:6250285/140‑1 (MQ=255)
                                                                                   aGAAAACAGTCGAGAAAGTATCTAAAATTTTTGGCACACAAACAAAGAACGCCGTTCACCGGCTTGCCAACGGAAAGACAAAAAAAGAGATCCGGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  2:5455990/140‑1 (MQ=255)
                                                                                     aTAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAACTGCTTGCCAACGGACAGACAAAAAAACAGATCCTGAAAACGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGt                                                        >  1:5714310/1‑140 (MQ=255)
                                                                                     aTAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGt                                                        >  1:945787/1‑140 (MQ=255)
                                                                                       aaaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtat                                                      >  2:6707950/1‑140 (MQ=255)
                                                                                          agcccacaaaGTATCAAAATTTTTTGGAAAAAAAAAAAACAAAGCATTACAACTGCTTGCCACCGCAAACACAAAAAAAGACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatg                                                   <  1:2074513/133‑1 (MQ=255)
                                                                                          aGTCGAGAAATTATAAAAACTTTTTGGCACACAAACAAAGAAACCAGTTCAAATGATTGCAAACGCAAACACAAAAAAAGCTAACCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatg                                                   <  1:3489060/140‑1 (MQ=255)
                                                                                              gataaaGTATCTAAATTTTGTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                               <  2:2472770/137‑1 (MQ=255)
                                                                                               agaAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTGGATGTATATGATGGt                                              >  2:1891982/1‑140 (MQ=255)
                                                                                                caaaGTATCTAAAATTTTGGGCAAACAAAAAAATAAAGCATTTAAAATGCTTGCCAACGTAAAGAAAAAAAAAGACATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                             <  1:5529921/139‑1 (MQ=255)
                                                                                                      aTCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                       >  1:6354753/1‑140 (MQ=255)
                                                                                                      aTCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                       >  1:1314794/1‑140 (MQ=255)
                                                                                                          aaaaTTTTGGGGAAAAAAACAAAGAAAGCCGTTCACTTGCTTGCCAACGGAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGt                                   <  2:5754800/140‑1 (MQ=255)
                                                                                                          aaaaTTTTGGGCAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGt                                   <  1:6600488/140‑1 (MQ=255)
                                                                                                           aaaTTTTTGGGAAACAACAAAATAAAGCTGTTCAAATGCTTCCAAACGGAAAGAAAAAAAAAGAGATCATGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtc                                  <  1:4286832/140‑1 (MQ=255)
                                                                                                              tttttGGGAAACAAACAAAGAAAGCACTTCAAATGCTTCCCAACGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatc                               <  2:606425/140‑1 (MQ=255)
                                                                                                              tttttGGGAAAAAAAAAAACAAAGCCGTTCAAATGCTTGCCACCGGAAAGACAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatc                               <  1:1386996/140‑1 (MQ=255)
                                                                                                               tttggccaaaaaaaaaaACAAACCAGTTCAAAGGCTTGCCAACGCAAACAAAAAAAAAGACATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatca                              <  1:5286999/133‑1 (MQ=255)
                                                                                                                tttgccaaacaaaaAAACAACCCAGTTCAAATGCTCGCCACCGCAACGACAAAAAAACAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  2:5915503/134‑1 (MQ=255)
                                                                                                                tttGGTAAAAAAACAAAGAAAGCTGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGCTCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  2:3570659/140‑1 (MQ=255)
                                                                                                                tttGGGAAACAAACAAAGAAAGCATTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGACCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  2:5138613/140‑1 (MQ=255)
                                                                                                                tttGGCAAACAAAAAAAGAAAGCAGTTCAAATGATTGCCAACTGAAAGACAAAAAAACAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  1:1796801/140‑1 (MQ=255)
                                                                                                                tttGGCAAAAAAACAAAGAAAGCTGTTAAACTGCTTGCCACCGCAACCAAAAAAAAAGAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  1:2472168/140‑1 (MQ=255)
                                                                                                                 ttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATg                            >  1:7130974/1‑140 (MQ=255)
                                                                                                                                agaaagaaGTTAAAAGCCTTGCAAACTGAAATACAAAAAAAGAGATCGTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATAATGGGTCTATCAGGGAGc             <  2:5367117/140‑1 (MQ=255)
                                                                                                                                   aagCAGGTCAAATGCTTGCAACCGGAAACACAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGt          <  2:6979343/140‑1 (MQ=255)
                                                                                                                                    agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGtc         >  2:7587230/1‑139 (MQ=255)
                                                                                                                                    agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTa         >  2:5973577/1‑140 (MQ=255)
                                                                                                                                       aGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAAt      <  2:366636/140‑1 (MQ=255)
                                                                                                                                        gTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc     <  2:3861309/140‑1 (MQ=255)
                                                                                                                                        gTTAAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc     <  2:2285948/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAC  >  NZ_CP034943/316123‑316401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: