Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,946,821 T→G 6.2% E191A (GAG→GCG)  EO946_RS20310 ← polysaccharide pyruvyl transferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,946,8210TG 6.2% 1603.5 / 15.1 602E191A (GAG→GCG) EO946_RS20310polysaccharide pyruvyl transferase family protein
Reads supporting (aligned to +/- strand):  ref base T (250/283);  new base G (63/2);  total (317/285)
Fisher's exact test for biased strand distribution p-value = 4.46e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 618 total aligned reads displayed.

CGTGTCTACCGTATCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTCTGTGTCTCACGCGCAGCAATCGTTAAGTTTGGATTTTGATGGTACGC  >  NZ_CP034943/3946682‑3946960
                                                                                                                                           |                                                                                                                                           
cGTGTCTACCGTATCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCt                                                                                                                                             >  1:2938982/1‑140 (MQ=255)
  tgtCTACCGTATCCAGCACCGTATCCGTACGCTCCGTATAAGTTGGTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCg                                                                                                                                           >  2:3607385/1‑140 (MQ=255)
    tCTACCGTATCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCgcg                                                                                                                                         >  1:6432072/1‑138 (MQ=255)
    tCTACCGTATCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCgcg                                                                                                                                         >  1:2680531/1‑138 (MQ=255)
    tCTACCGTATCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAg                                                                                                                                         >  2:5520793/1‑140 (MQ=255)
    tCTACCGTATCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTATCCTTATCCGCTCTTAAAATAAAGAGAACGCAGTCCCGGGCGAg                                                                                                                                         >  1:4204817/1‑140 (MQ=255)
           tATCCAGCACCGTATCGGTACGCTCTGTGTAAGTTGTTTTTTCTGCCCATTGCTTCATCTGTGAAATAAAATCTTCGTCAGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTcgc                                                                                                                                  >  2:1883237/1‑140 (MQ=255)
           tATCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAATAGAACGCCGTCCCGCTCGAGCTCTcgc                                                                                                                                  >  2:5460289/1‑140 (MQ=255)
           tATCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTcgc                                                                                                                                  >  2:5825575/1‑140 (MQ=255)
           tATCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTcgc                                                                                                                                  >  2:796928/1‑140 (MQ=255)
            aTCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCGGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTcgcg                                                                                                                                 <  2:2916501/140‑1 (MQ=255)
             tCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCgg                                                                                                                                >  2:1926077/1‑140 (MQ=255)
             tCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCgg                                                                                                                                >  2:566236/1‑140 (MQ=255)
             tCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCgg                                                                                                                                >  2:919949/1‑140 (MQ=255)
             tCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCgg                                                                                                                                >  2:2062189/1‑140 (MQ=255)
                aGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGcac                                                                                                                             <  2:5592133/140‑1 (MQ=255)
                aGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGcac                                                                                                                             >  2:3005901/1‑140 (MQ=255)
                aGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGcac                                                                                                                             <  2:1026075/140‑1 (MQ=255)
                aGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCGCCAGCACgcgccccac                                                                                                                             >  1:17195/1‑135 (MQ=255)
                 gCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGcaca                                                                                                                            <  1:702967/140‑1 (MQ=255)
                 gCACCGTATCCGTACGCTACGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTAGCGGcaca                                                                                                                            <  2:2156166/140‑1 (MQ=255)
                     cGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGc                                                                                                                                        <  1:1215281/124‑1 (MQ=255)
                     cGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAatat                                                                                                                        >  2:4580588/1‑140 (MQ=255)
                     cGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAatat                                                                                                                        >  2:6701622/1‑140 (MQ=255)
                       tATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGGGCGAGCTCGCGCGGCACAatatac                                                                                                                      >  2:702967/1‑138 (MQ=255)
                       tATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTcg                                                                                                                                   <  2:638599/127‑1 (MQ=255)
                       tATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTcg                                                                                                                                   >  1:638599/1‑127 (MQ=255)
                       tATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAAAATca                                                                                                                      >  1:3082658/1‑139 (MQ=255)
                       tATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCGCGCGCGCGCGCGGCCCAaaatac                                                                                                                      >  1:5794135/1‑138 (MQ=255)
                       tATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCGCGAGCTCTCGCGGCACCAAATcc                                                                                                                      >  1:5311326/1‑140 (MQ=255)
                         tCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCTAGCTCTCGCGGCACAATATCCaa                                                                                                                    <  1:6281629/140‑1 (MQ=255)
                         tCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCaa                                                                                                                    <  1:6745804/140‑1 (MQ=255)
                             tACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCaaagaa                                                                                                                <  2:1243671/140‑1 (MQ=255)
                              aCGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCaaagaaa                                                                                                               <  2:5023738/140‑1 (MQ=255)
                               cGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCaa                                                                                                                    <  2:2267847/134‑1 (MQ=255)
                               cGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCaa                                                                                                                    >  1:2267847/1‑134 (MQ=255)
                               cGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAAAATCCaaagaaag                                                                                                              >  2:337971/1‑140 (MQ=255)
                                 cTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAAAATCCAAAGAAAGCa                                                                                                            >  2:6862186/1‑140 (MQ=255)
                                   ccGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCGCGCGCGCTCGGCGCCCCAAATACAAAAAAAGCAcc                                                                                                          >  1:4393776/1‑140 (MQ=255)
                                    cGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGACCGCCGTCCCGCTCGCGCGCTCGGGGCACAATATCCAAAGAAAGCAcac                                                                                                         >  1:2738402/1‑138 (MQ=255)
                                    cGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGGACAAAATCCAAAGAAAGCACCa                                                                                                         >  2:5174165/1‑140 (MQ=255)
                                    cGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCAcc                                                                                                          <  1:3539302/139‑1 (MQ=255)
                                    cGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCa                                                                                                         >  2:4157805/1‑140 (MQ=255)
                                    cGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCa                                                                                                         >  2:3845599/1‑140 (MQ=255)
                                     gTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCCAAGAAAGCAccct                                                                                                        >  2:5924341/1‑138 (MQ=255)
                                      tataAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACCAAATCCAAATAAAGCACCaag                                                                                                       >  2:1784554/1‑138 (MQ=255)
                                        taAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTc                                                                                                     <  1:1039279/140‑1 (MQ=255)
                                        taAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTc                                                                                                     <  1:6176000/140‑1 (MQ=255)
                                         aaGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTcc                                                                                                    <  1:4753810/140‑1 (MQ=255)
                                         aaGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTcc                                                                                                    <  2:616383/140‑1 (MQ=255)
                                          aGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCg                                                                                                   <  1:553790/140‑1 (MQ=255)
                                          aGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCg                                                                                                   <  2:7148703/140‑1 (MQ=255)
                                            ttgttTTTTCTGCCCATTTCCTCATCTGTGGAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGgt                                                                                                 >  2:4334591/1‑140 (MQ=255)
                                            ttgttTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGgt                                                                                                 >  2:1594340/1‑140 (MQ=255)
                                              gttTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGATCGCTCGCGGCACAATATCCAAAGAAACCACCATGTCCGgtgt                                                                                               >  2:1334072/1‑140 (MQ=255)
                                              gttTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTcg                                                                                                                                   <  1:1829219/104‑1 (MQ=255)
                                              gttTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCGCGAGCTCTCGCGGCACAATATCCAAAGAAAACACCAACTCCGgggt                                                                                               >  1:5118699/1‑140 (MQ=255)
                                                tttttCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTaa                                                                                             <  1:771139/140‑1 (MQ=255)
                                                 ttttCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTAGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTATAGAGGCACAATATCCAAACAAAGCACAAAGTCAGGTGTaaa                                                                                            >  2:4044781/1‑140 (MQ=255)
                                                    tCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGAt                                                                                         <  1:1110872/140‑1 (MQ=255)
                                                        cccATGTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATcaca                                                                                     <  1:1580717/140‑1 (MQ=255)
                                                         ccATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACAt                                                                                    <  2:3958531/140‑1 (MQ=255)
                                                                  cATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGGTCGAGCTAACCCGGCACCCTCTACAAAGAAAGCAACAAGTAAGGTGGAAAGATAACATTGGAGTGGa                                                                           >  1:4536198/1‑140 (MQ=255)
                                                                  cATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGATCTCGCGGCACAAATTCCAAAGAAACCACCATGTCCGGTGTAAAGATCACATTGGAGTTGa                                                                           >  2:7484301/1‑140 (MQ=255)
                                                                    tCTTTGAAATAAAATCGTCGTCGGTCACTTTCTCCTGAGCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAg                                                                         <  2:5979748/140‑1 (MQ=255)
                                                                     cTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGCAgg                                                                        >  2:4623220/1‑140 (MQ=255)
                                                                      tgttatataataTCTTAGTCTGTGACTGTCTACTTATCCGCTCTTAAAATAAATAGTAAGTAGTACCGCTTGAGCGCTCGATGCACACTTTCCAAAGAACTCAGCATGTCGGGTTTAAAGTTCTCTTTGGAGTTGAATGt                                                                       <  2:2758263/134‑1 (MQ=255)
                                                                      tgtgAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGt                                                                       <  2:487591/140‑1 (MQ=255)
                                                                        tgAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACAATGGAGTTGAAGGTCt                                                                     >  2:2512597/1‑140 (MQ=255)
                                                                        tgAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGGAAAGATCACATTGGAGTTGAAGGTCt                                                                     >  2:6474615/1‑140 (MQ=255)
                                                                         gAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCtt                                                                    <  1:4974897/140‑1 (MQ=255)
                                                                               aaaTCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTac                                                              <  1:1412506/140‑1 (MQ=255)
                                                                               aaaTCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTac                                                              <  2:6573586/140‑1 (MQ=255)
                                                                               aaaTCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTac                                                              <  1:7174911/140‑1 (MQ=255)
                                                                               aaaTCTTCGTCGGTCAATTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTAGAGCTCTCGCGGCACAAGAACCAAAGAAAGCACCATGTCCGGTGTAACGATCACATTGGAGTTGAAGGTCTTTTTTac                                                              <  1:3679260/140‑1 (MQ=255)
                                                                                aaTCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTaca                                                             <  1:7607075/140‑1 (MQ=255)
                                                                                 aTCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTacaa                                                            <  2:729133/140‑1 (MQ=255)
                                                                                 aTCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTacaa                                                            <  2:1112134/140‑1 (MQ=255)
                                                                                 aTCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTacaa                                                            <  1:3845599/140‑1 (MQ=255)
                                                                                  tCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTacaac                                                           >  2:6270774/1‑140 (MQ=255)
                                                                                            tCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCACAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTc                                                 <  1:6547390/140‑1 (MQ=255)
                                                                                                       ttATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTCTGTGTCTCAcg                                      <  2:3279261/140‑1 (MQ=255)
                                                                                                        tATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCGCGAGCTCTCGCGGCACAAAATCCAAACAAAGCACCAAGTCCGGTGTAAAGATCACAATGGAGGTGGAGGTGTTTTTTACAAAATACACCCTCGGTGTCTCAcgc                                     >  1:2467944/1‑140 (MQ=255)
                                                                                                          tCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGAt                                                                                         <  1:7300671/86‑1 (MQ=255)
                                                                                                          tCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCGGGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCCGTGTAAAGATCACATTGGAGGTGAAGGTCTTTTTTAAAAAATACAGCGTCTGTGTGTCACgcgc                                   >  1:2375225/1‑140 (MQ=255)
                                                                                                            cGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCACAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTCTGTGTCTCACGCgcag                                 <  2:2467392/140‑1 (MQ=255)
                                                                                                              ctctTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCCAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTCTGTGTCTCACGCgcagca                               >  2:4405778/1‑140 (MQ=255)
                                                                                                              ctctTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTCTGTGTCTCACGCgcagca                               >  2:1456916/1‑140 (MQ=255)
                                                                                                              ctctTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATACAAAGAAAGCACCATGTCCGGTGTAAAGAACACAATGGAGTTGAAGGTCTTTTTTACAAAATACACCGTCTGTGTCTCACCCgcagca                               >  2:4727900/1‑140 (MQ=255)
                                                                                                                         aaGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTCTGTGTCTCACGCGCAGCAATCGTTAAGtt                    <  1:2060764/140‑1 (MQ=255)
                                                                                                                              aaCGCCGTCCCGCTCGATCTATCGCGGCACAATATCCTAAGAAAGCACGATGTCAGTTGTAAAGATCACATTTGAGTTGAAGGTATTTTTTACAACATCCAGCGTCTTTGTCTCACGCGAAGCAATCGTTAAGTTTGGAt               <  1:1905491/140‑1 (MQ=255)
                                                                                                                              aaCGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATcaca                                                                                     >  2:5305018/1‑70 (MQ=255)
                                                                                                                                   agTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCATCGTCTGTGTCTCACGCGCAGCAATCGTTAAGTTTGGATTTTGa          <  1:2154837/139‑1 (MQ=255)
                                                                                                                                    gTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTCTGTGTCTCACGCGCAGCAATCGTTAAGTTTGGATTTTGAt         <  1:4044781/140‑1 (MQ=255)
                                                                                                                                    gTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTCTGTGTCTCACGCGCAGCAATCGTTAAGTTTGGATTTTGAt         <  1:5668374/140‑1 (MQ=255)
                                                                                                                                    gTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTCTGTGTCTCACGCGCAGCAATCGTTAAGTTTGGATTTTGAt         <  1:5979366/140‑1 (MQ=255)
                                                                                                                                    gTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTCTGTGTCTCACGCGCAGCAATCGTTAAGTTTGGATTTTGAt         <  2:1689859/140‑1 (MQ=255)
                                                                                                                                    gTCCCGCTCGAGCTCTCGCGGCACAAGATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTCTGTGTCTCACGCGCAGCAATCGTTAAGTTGGGATTTTGAt         <  1:54350/140‑1 (MQ=255)
                                                                                                                                          cTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATAACATTGGGGTTGACGGTCTTTTTTACAACATCCAGCGGCTGTGTCTCACGCGCCGCACTCGTTAAGTTTGGATTTTGATGGTagg   >  1:3336403/1‑138 (MQ=255)
                                                                                                                                          cTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACAATGTCCGGTGTAAAGATCACATTGGAGTTGAATCTCTTTTTTAAAACATCCAGAGTCTGTGTCTCACGCGCAGCAATCGTGAAGTTTGGATTTTTTTGGTaag   >  1:882166/1‑138 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CGTGTCTACCGTATCCAGCACCGTATCCGTACGCTCCGTATAAGTTGTTTTTTCTGCCCATTTCCTCATCTGTGAAATAAAATCTTCGTCGGTCACTTTCTCCTTATCCGCTCTTAAAATAAAGAGAACGCCGTCCCGCTCGAGCTCTCGCGGCACAATATCCAAAGAAAGCACCATGTCCGGTGTAAAGATCACATTGGAGTTGAAGGTCTTTTTTACAACATCCAGCGTCTGTGTCTCACGCGCAGCAATCGTTAAGTTTGGATTTTGATGGTACGC  >  NZ_CP034943/3946682‑3946960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: