Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,976,114 G→T 6.7% A31E (GCA→GAA)  rlmH ← 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,976,1140GT 6.7% 1452.4 / 26.5 562A31E (GCA→GAA) rlmH23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH
Reads supporting (aligned to +/- strand):  ref base G (223/284);  new base T (52/3);  total (275/287)
Fisher's exact test for biased strand distribution p-value = 3.46e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 567 total aligned reads displayed.

CGAGGGCGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAA  >  NZ_CP034943/3975977‑3976253
                                                                                                                                         |                                                                                                                                           
cGAGGGCGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCg                                                                                                                                           <  1:3445710/140‑1 (MQ=255)
      cGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGcc                                                                                                                                     >  2:3192431/1‑140 (MQ=255)
              tGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGTTGATGTCGATTTTTGCGTAGGCCGAAAGTCg                                                                                                                             >  1:5461850/1‑140 (MQ=255)
              tGGGCGTCGGGGCGGATTTTGGATAGGATGCGGTCGCCTTCGTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTGCCGGCGCTTTTTCGTCCGGGAGTGCGATGATGTCGATTTTTGCGTATGCCGAAAGGCg                                                                                                                             >  2:1750044/1‑140 (MQ=255)
               gggCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATTATGTCGATTTTTGCGTAGGCCGAAAGTCGt                                                                                                                            >  2:7278425/1‑140 (MQ=255)
               gggCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGt                                                                                                                            >  2:2281287/1‑140 (MQ=255)
               gggCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTGTGTCTGTTTTGATTTTCATGTCAGGTTCGCTTAGGGTTTCCGGGTCGTTTTGGTGCGGGAGGGCGATGATGGCGATTTTTGCGTAGGGCGaaatgcgt                                                                                                                            >  2:6183293/1‑135 (MQ=255)
                     cGGGGCTGATTTTGGATAGGATGCGGTGGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGGTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCgtg                                                                                                                      >  2:2921318/1‑140 (MQ=255)
                     cGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCgtg                                                                                                                      <  2:2352539/140‑1 (MQ=255)
                       gggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTa                                                                                                                    <  2:5943893/140‑1 (MQ=255)
                       gggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTa                                                                                                                    <  1:6592195/140‑1 (MQ=255)
                       gggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTa                                                                                                                    <  1:2772412/140‑1 (MQ=255)
                       gggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTa                                                                                                                    <  1:3647331/140‑1 (MQ=255)
                       gggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTa                                                                                                                    <  1:7682411/140‑1 (MQ=255)
                       gggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGAGTTTTGCGTAGGCCCTAAGTCGTTTCGTGTa                                                                                                                    >  2:5540175/1‑140 (MQ=255)
                          cTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAAGCCGAAAGTCGTTTCGTGTAttc                                                                                                                 >  2:355360/1‑140 (MQ=255)
                            gATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCAGGCGCTTTTTCGTCCGGGAGTTCGAAGATGTAGATTTTTTCGTAGGCCGAAATTCGTTTCGGGTAttctt                                                                                                               >  2:1951103/1‑140 (MQ=255)
                                 tGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATg                                                                                                          >  2:1659961/1‑140 (MQ=255)
                                 tGGATAGGATGCGGTCGCCTTCTTTGACTTTTATGAATTTCATGTCCTGGACGCTTAGGTTTTCCGGCGCATTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTACaaag                                                                                                          >  2:7461598/1‑138 (MQ=255)
                                  ggATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTAGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTGGTGTATTCTTCAATGc                                                                                                         >  2:5060875/1‑140 (MQ=255)
                                  ggATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTAGTGTATTCTTCAAAGc                                                                                                         >  1:4945512/1‑140 (MQ=255)
                                  ggATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTTGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAAttc                                                                                                         >  1:6086414/1‑138 (MQ=255)
                                  ggATAGGATGCGGTCGCCTTCTTGGGCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAATTCGTTTCGGGTATTCTTCAATGc                                                                                                         >  1:2851160/1‑140 (MQ=255)
                                       ggATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTTGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTGGATGCTGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgg                                                                                                    >  1:3375825/1‑139 (MQ=255)
                                       ggATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCATGGTCGCGTATGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgc                                                                                                    >  2:4757261/1‑140 (MQ=255)
                                        gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTGGTCCCGGAGTTCGATGATGTCGCTTTTTTCGTATGCCGAAAGTCCTTTCGGTTATTATTGAATGCcttggg                                                                                                   >  1:2409377/1‑138 (MQ=255)
                                        gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCGTTGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGGGTATGCCGAAAGTGGGTTCGGTTATTATTAAATTCcatggt                                                                                                   >  1:3929989/1‑138 (MQ=255)
                                          tGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgcttg                                                                                                 <  1:1474843/140‑1 (MQ=255)
                                             ggTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAgg                                                                                              >  2:5352759/1‑140 (MQ=255)
                                              gTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTAGTGTATTCTTCAATGCATTGCTTGAGGt                                                                                             >  2:4510498/1‑140 (MQ=255)
                                                  ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGGTTCGTGTATTCTTCAATGCCTTGCTGGAGGTAttt                                                                                         >  1:5657168/1‑140 (MQ=255)
                                                  ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCGTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCGTGCTTGAGGTAttt                                                                                         >  2:6540748/1‑140 (MQ=255)
                                                  ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATGATTTCGATTTTTGCGTATGCCGAAAATCGTTTCGTGTATTATTAAATGGCTTGCTTGAGGTAttt                                                                                         >  1:3482406/1‑140 (MQ=255)
                                                         tGTCTTTTATGATTTTCATGTCCTGGTCGCTTCGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCCATTATGTCGATTTTTGCGTAGGGCGACAGTGGTTTGGTGTAGTATTAAATTCCGTGGTTGAGGtatttttatttt                                                                                  >  1:4208733/1‑140 (MQ=255)
                                                         tGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTAGTCCCGGATTTACATTATGTCGATTTTTTCGTATGCCGAAAATAGTTTAGTGTAATATTAAATTCCTTGCTTTATGtatttttatttt                                                                                  >  1:7196720/1‑140 (MQ=255)
                                                               ttATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTAGTCCGGGCGTTCGATGTTGTCGATTTTTGGGTTGGCCCAAATTGGTTTGGTGTATTCTTAAATTCCTTGCGTGAGGTATTTTTGTTTTATtttt                                                                            >  1:2633704/1‑140 (MQ=255)
                                                               ttATGATTTTCATGTCCTGGTCGCGTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTAGATGATGTCGATTTTTGCGTAGGCCCAAAGTAGTTTCGTGTATTAATCAAAGCCGTTCTTGAGGTAATTTTCTTTTAGtttt                                                                            >  1:701380/1‑140 (MQ=255)
                                                                tATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTGGGCCCGGAGTTCGATGATGTGGATTTTTGCGTAGGCCGAAAGTGGTTTCGTGTCTTCTTCAATTAGTTTCTGTAGGTATTTTTCTTTTAGTTTTc                                                                           >  1:6556507/1‑140 (MQ=255)
                                                                tATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAATTCGTTTCGTGTATTCTTAAATTCCTTGCTTGAGGTATTTTTCTTTTAGttttg                                                                           >  1:7388659/1‑139 (MQ=255)
                                                                tATGATTTTCATGTCCTGGTCGCGTAGGTTTTCCGGCGCGTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGGTTCGTGTTTTCTTGAATGCCTTGCTTGGGGTATTTTTCTTTTAGttttg                                                                           >  1:6030248/1‑139 (MQ=255)
                                                                tATGATTTTCATGTCCTGGTCGCGTAGGTTTTACGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATTCCATGCTTGAGGTATTTTTTTTTTATtttta                                                                           >  1:579871/1‑139 (MQ=255)
                                                                    aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                       <  2:1289967/140‑1 (MQ=255)
                                                                    aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                       <  1:4088116/140‑1 (MQ=255)
                                                                       ttCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTGGTTGGCCGAAAGTCGTTTGGGGTATTCTTCAATGCCGTGGGTGAGGTATTTTTATTTTTTTTTTGCGAtttt                                                                    >  1:217246/1‑138 (MQ=255)
                                                                       ttCATGTCCTGGTCGCGTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTCGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGt                                                                    <  2:890309/140‑1 (MQ=255)
                                                                         cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                  <  1:4379567/140‑1 (MQ=255)
                                                                         cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                  <  1:3324011/140‑1 (MQ=255)
                                                                          aTGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTcac                                                                 <  1:2995037/140‑1 (MQ=255)
                                                                            gTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACaa                                                               <  2:2200816/140‑1 (MQ=255)
                                                                                 ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                          <  2:3662914/140‑1 (MQ=255)
                                                                                  gTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTAAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTACGATTGTCACAATATTGa                                                         >  2:4947236/1‑140 (MQ=255)
                                                                                  gTCGCTTAGGTTTTACGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTGGTGTATTCTTCAATTCCTTGCTTTAGGTATTTTTGTTTTAGTTTTCCGATTGTCACAATAtttt                                                         >  1:5221649/1‑138 (MQ=255)
                                                                                   tCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGat                                                        <  1:1361896/140‑1 (MQ=255)
                                                                                   gcgcggcggTGTTCCGGCGCTTTTTCGGCCGGGAGGTCGCTGCGGTCGATTGTTGCGTAGGCCGAAAGTCGTTGCGTGTATACGTCAATGCCTTGCTTGAGGTATTTTTCTTGTAGTTTTCCGATTGGCACAATATTGat                                                        <  2:7117880/133‑1 (MQ=255)
                                                                                    cGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTAAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTACGATTGTCAAAATATTtata                                                       >  2:3019886/1‑140 (MQ=255)
                                                                                       ttAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCGTGCTTGAGGTATTTTTGTTTTAGTTTTCCGATTTTCACAATATTGATAtta                                                    >  2:3420589/1‑139 (MQ=255)
                                                                                         aGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTGTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTcac                                                  <  2:7196720/140‑1 (MQ=255)
                                                                                          tgTTTTCCGGCGCATTTTAGTCCGGGCGGTCGATGATGTCGATTTTTGCGTAGACCGAAAGTCGTTTAGTGTATTCTTCAATTCCATGCTTGAGGTATTTTTATTTTAGTTTTCCGATTTTCACAAAAATTATATTcaca                                                 >  2:5681087/2‑140 (MQ=255)
                                                                                           gTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAg                                                <  1:63245/140‑1 (MQ=255)
                                                                                             tttCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGt                                              <  2:5199566/140‑1 (MQ=255)
                                                                                             tttCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGt                                              <  2:250162/140‑1 (MQ=255)
                                                                                              ttacGGCGCTTTTTAGTGCGGGAGTTAGATGATGTCGAATTTTTCGTAGGCCGAAAGTCGTTTGGTGTATTCTTCAATGGCTTTGTTTATGTATTTTTGTTTTAGTTTTACGATTTTGACAAAAATGATATTCAAACGTc                                             >  2:6005/4‑140 (MQ=255)
                                                                                              ttCCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGGTTCGTGTATTCTTAAATTCCGTGCTTTAGGTATTTTTATTTTAGTTTTACCATTGTAACAAAATTTATATTAACAggga                                             >  2:6744549/1‑138 (MQ=255)
                                                                                               tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                            >  1:1897922/1‑140 (MQ=255)
                                                                                               tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCGTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                            >  1:945141/1‑140 (MQ=255)
                                                                                               tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTAAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCAAAGGTCa                                            >  1:4641691/1‑140 (MQ=255)
                                                                                               tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTTATATTCACAGGTCa                                            >  1:3857913/1‑140 (MQ=255)
                                                                                                ccGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCGTGCTTGAGGGATTGTTCTTTTAGTTTTCCGATTGTCACACTATTGATATTCACAGGTCAt                                           >  1:2234935/1‑140 (MQ=255)
                                                                                                                 gggAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACaa                          >  1:6969037/1‑140 (MQ=255)
                                                                                                                  ggAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGGTTCGTGTATTCTTCAATGCCTTGGTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAg                         <  2:5911703/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCTATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  1:7298138/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:4310144/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:6937383/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:4202534/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:3082964/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:1427099/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  1:7504413/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  1:4986477/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  1:3143547/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:7561500/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  1:1008926/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTAGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  1:109241/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGAGTAGGCCGAAAGTCGTTTCGTGTAGTCTTCAATTCCGTGCTTGAGGTATTTTTCTTTTAGTTTTCCGTTTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:7652457/140‑1 (MQ=255)
                                                                                                                       tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTTTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                    <  2:6680356/140‑1 (MQ=255)
                                                                                                                       tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                    <  2:4700000/140‑1 (MQ=255)
                                                                                                                        cGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTc                   >  1:6886787/1‑140 (MQ=255)
                                                                                                                             atgTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCatata              >  2:639297/1‑140 (MQ=255)
                                                                                                                             atgTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCatata              >  2:1012096/1‑140 (MQ=255)
                                                                                                                               gTCGATTTTTGCGTAGGCCGACAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAAATAGTTATTCATATAtg            <  2:3744291/140‑1 (MQ=255)
                                                                                                                               gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCACTGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg            <  1:4839024/140‑1 (MQ=255)
                                                                                                                               gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg            <  2:3944152/140‑1 (MQ=255)
                                                                                                                               gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg            <  1:158306/140‑1 (MQ=255)
                                                                                                                               gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg            >  2:1172489/1‑140 (MQ=255)
                                                                                                                               gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg            >  2:2677083/1‑140 (MQ=255)
                                                                                                                                tCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtgt           >  2:20743/1‑140 (MQ=255)
                                                                                                                                 cGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtgtt          <  1:1227095/140‑1 (MQ=255)
                                                                                                                                  gATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                          <  1:5081684/91‑1 (MQ=255)
                                                                                                                                    tttttGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCATAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTc       <  1:5362637/140‑1 (MQ=255)
                                                                                                                                      tgtGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCca     <  1:6785571/138‑1 (MQ=255)
                                                                                                                                        tGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCcaca   >  2:3258048/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
CGAGGGCGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAA  >  NZ_CP034943/3975977‑3976253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: