Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,976,145 C→A 5.4% E21* (GAA→TAA)  rlmH ← 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,976,1450CA 5.4% 1321.5 / 5.3 507E21* (GAA→TAA) rlmH23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH
Reads supporting (aligned to +/- strand):  ref base C (183/264);  new base A (50/2);  total (241/266)
Fisher's exact test for biased strand distribution p-value = 1.07e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 524 total aligned reads displayed.

ATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAG  >  NZ_CP034943/3976006‑3976284
                                                                                                                                           |                                                                                                                                           
 ttttGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCa                                                                                                                                            <  1:5690364/140‑1 (MQ=255)
          ggATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTATGTTTTACGGCGCGTTTTAGTCCGGGAGTTCGATGATTTCGATTTTTTGGTAGGCCGAAAGTGGTTTGGTGTATTGTTAAATTCctttg                                                                                                                                   >  1:38394/1‑138 (MQ=255)
          ggATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTGCGGGAGTTCGATGATGTCGATTTTTTGGTAGGCCGAAAGTCGTTTGGTGTATTATTAAATTActtgc                                                                                                                                   >  1:4148124/1‑140 (MQ=255)
          ggATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCATGGTCGCGTATGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgc                                                                                                                                   >  2:4757261/1‑140 (MQ=255)
           gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTTGGTTTTCCGGCGCGTTTTGGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTGGGTTTGTGTATTTTTAAATTCctttgt                                                                                                                                  >  1:6077164/1‑137 (MQ=255)
           gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgct                                                                                                                                  >  2:6971451/1‑140 (MQ=255)
           gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgct                                                                                                                                  >  2:5353320/1‑140 (MQ=255)
           gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCGTAGGTTTTCCGGCGCGTTTTGGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTAGTTTCGGGTATTATTCAAAGCcgtgcg                                                                                                                                  >  1:4921714/1‑139 (MQ=255)
             tGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCATTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgcttg                                                                                                                                <  1:3199183/140‑1 (MQ=255)
                ggTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAgg                                                                                                                             >  2:1694586/1‑140 (MQ=255)
                ggTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCTTGCTTGAgg                                                                                                                             >  2:667529/1‑140 (MQ=255)
                     ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTAttt                                                                                                                        >  2:416566/1‑140 (MQ=255)
                     ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTAttt                                                                                                                        >  2:1126529/1‑140 (MQ=255)
                     ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATGCCTTGCTTGAGGTAttt                                                                                                                        >  2:5857931/1‑140 (MQ=255)
                     ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAAAGCCTTGCGTGAGGTAttt                                                                                                                        >  2:6721178/1‑140 (MQ=255)
                     ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCGTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCGTGCTTGAGGTAttt                                                                                                                        >  2:6540748/1‑140 (MQ=255)
                     ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCGTAGGTTTTCCGGCGCTTTTTGGTCCGGGAGTTCGATGATGTCGATTTTTTCGTATGCCGAAAGTAGTTTCGTGTATTATTAAATTCCTTGCTTGAGGTAttt                                                                                                                        >  1:297669/1‑140 (MQ=255)
                     ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCGGAGGGTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGGGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGTGGTAttt                                                                                                                        >  1:5657153/1‑140 (MQ=255)
                     ccTTCTTTGTCTTTTATGATTTTCATGTCCGGGTCGCTTAGGTTTTCCGGCGCTTTTTCGGCCGGGAGTTCGATTATGTCGATTTTTTCGTAGGCCGAAAGTCTTTTCGTGTATTCTTCAATGCCGTGCTTGAGGTAttt                                                                                                                        >  2:1900510/1‑140 (MQ=255)
                            tGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTAAGGCGCTTTTTCTTCCCGGAGTTCGAAGATGTAGATTTTTGCGTAGGGCGAAAATCGTTTAGTGTATTCATCAATTCCTGTGGTGAGGTAattttatttt                                                                                                                 >  1:6844589/1‑140 (MQ=255)
                              tCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACCGCGCTTTTTCGTCCGGGACTTCGATGAAGTAGATTTTTGCCTAAGCCGAAACTCGTTTCGGGTATTATTCAAGGCCTTGCGTGAGGTAGTTTTCTTTTAg                                                                                                               >  2:2834304/1‑140 (MQ=255)
                                ttttATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTGGTCCGGGAGTTCGATTATGTCGATTTTTTCGTTTTCCGAAATTCGTTTGGTGTATTTTTAAATTCCTTTGTTGAGGTAtttttttttttttt                                                                                                             >  1:6589509/1‑136 (MQ=255)
                                ttttATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTGCGGGAGTTCGATGATGTGGATTTTTGCGTTGGCCGAAACTGGTTTAGGGTATTCTTCAATGGCGTTGTTGAGTTATTTTTGTTTTAGtt                                                                                                             >  1:6793228/1‑140 (MQ=255)
                                  ttATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTAGTCCGGGCGTTCGATGTTGTCGATTTTTGGGTTGGCCCAAATTGGTTTGGTGTATTCTTAAATTCCTTGCGTGAGGTATTTTTGTTTTATtttt                                                                                                           >  1:2633704/1‑140 (MQ=255)
                                   tATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATTTTGTAGATTTTTGCGTAGGCCGAAAGTCGTTTCGGTTATTCTTCAATGCCTTTCTTGTGGTATTTTTATTTTTGttttt                                                                                                          >  1:3328791/1‑139 (MQ=255)
                                   tATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCCCATTTTCGTACCGGAGTTCGATGATGTCGATTTTTGCTTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGGTTTAGGTATTTTTCTTTTAGTTTTc                                                                                                          >  2:233246/1‑140 (MQ=255)
                                   tATGATTTTCATGTCCTGGTCGCGTAGGTTTTACGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATTCCATGCTTGAGGTATTTTTTTTTTATtttta                                                                                                          >  1:579871/1‑139 (MQ=255)
                                       aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                                                      <  1:6958376/140‑1 (MQ=255)
                                       aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                                                      <  2:2441329/140‑1 (MQ=255)
                                          ttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGt                                                                                                   <  2:6077164/140‑1 (MQ=255)
                                          ttCATGTCCTGGTCGCTTAGGTTTTCCGGCGATTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGt                                                                                                   <  2:347705/140‑1 (MQ=255)
                                          ttCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTGGTTGGCCGAAAGTCGTTTGGGGTATTCTTCAATGCCGTGGGTGAGGTATTTTTATTTTTTTTTTGCGAtttt                                                                                                   >  1:217246/1‑138 (MQ=255)
                                          ttCATGTCCTGGTCGCGTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTCGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGt                                                                                                   <  2:890309/140‑1 (MQ=255)
                                            cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                 <  1:4700689/140‑1 (MQ=255)
                                            cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                 <  1:7256750/140‑1 (MQ=255)
                                            cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                 <  1:6297030/140‑1 (MQ=255)
                                            cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                 <  2:1205187/140‑1 (MQ=255)
                                            cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                 <  2:3054184/140‑1 (MQ=255)
                                            cATGTCCTGGTCGATTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                 <  1:7379814/140‑1 (MQ=255)
                                                  cTGGTCGCGTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTAGATTTTTTCGTAGGCCGAAAGTCGTTTCGTGTATTCTTAAATGCCTTGCTTTAGGTATTTTTATTTTAGTTTTCCGATTGTCACAatat                                                                                           >  2:3245458/1‑140 (MQ=255)
                                                    ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                         <  2:439356/140‑1 (MQ=255)
                                                    ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                         <  1:7592764/140‑1 (MQ=255)
                                                    ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                         <  2:3662914/140‑1 (MQ=255)
                                                    ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGGTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTGTCCGATTGTCACAATATTg                                                                                         <  1:649554/140‑1 (MQ=255)
                                                     gTCGCTTAGGTTTTGCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTAAATTCCTTGGTTGAGGTATTTTTATTTTATTTTTAAGATTTTAACAATAttta                                                                                        >  2:635578/1‑138 (MQ=255)
                                                     gTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCCTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACACTATTGa                                                                                        >  2:1536551/1‑140 (MQ=255)
                                                     gTCGCGTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGGGTAGGCCGAAAGTCGTTTCGTGTATTCTTAAAAGGCTTGGTTGAGGTATTTTTATTTTAGTTTTACCATTGTCACAAAATTGa                                                                                        >  1:7163308/1‑140 (MQ=255)
                                                     gTCGCGTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGa                                                                                        >  2:5213701/1‑140 (MQ=255)
                                                       cGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTAACAATATTGata                                                                                      >  2:5024007/1‑140 (MQ=255)
                                                       cGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCACTGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGata                                                                                      <  1:3894179/140‑1 (MQ=255)
                                                       cGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGata                                                                                      <  1:1445437/140‑1 (MQ=255)
                                                           tAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTca                                                                                  <  1:7040949/140‑1 (MQ=255)
                                                                tttCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGt                                                                             >  2:7131526/1‑140 (MQ=255)
                                                                tttCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGt                                                                             >  2:2132198/1‑140 (MQ=255)
                                                                 ttCCGGCGCTTTTTCGTCCGGGAGTTCGATGATTTCGATTTTTTCGTAGGCCGAAAGTCGTTTGGTGTATTATTAAAATCCTTTCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAAAAATTATATTAAAAGGta                                                                            >  2:4308028/1‑139 (MQ=255)
                                                                  tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTGGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                                                           >  1:5063769/1‑140 (MQ=255)
                                                                    cGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATc                                                                         >  1:4224824/1‑140 (MQ=255)
                                                                       cgcTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATcccc                                                                      <  1:3377908/140‑1 (MQ=255)
                                                                         cTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGTTATTTTTCTTTTAGTTTTCCGATTGTCACAAGATTGATATTCACAGGTCATCCCCAc                                                                    >  2:3368825/1‑140 (MQ=255)
                                                                            tttCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTATTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACttt                                                                 >  1:4029677/1‑140 (MQ=255)
                                                                                     ggAGTTCGATGATGTCGTTTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAg                                                        <  2:2564327/140‑1 (MQ=255)
                                                                                     ggAGTTCGATGATGTCGATTTTTGCGTAGGCCTATAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAg                                                        <  2:7634668/140‑1 (MQ=255)
                                                                                       aGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGtt                                                      <  2:5967225/140‑1 (MQ=255)
                                                                                        gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                     <  1:1343049/140‑1 (MQ=255)
                                                                                        gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                     <  1:1008926/140‑1 (MQ=255)
                                                                                        gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                     <  1:4952271/140‑1 (MQ=255)
                                                                                        gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                     <  1:1189235/140‑1 (MQ=255)
                                                                                        gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                     <  2:1394504/140‑1 (MQ=255)
                                                                                        gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                     <  1:2844181/140‑1 (MQ=255)
                                                                                        gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                     <  2:6844589/140‑1 (MQ=255)
                                                                                        gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                     <  2:2203054/140‑1 (MQ=255)
                                                                                        gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                     <  1:3455298/140‑1 (MQ=255)
                                                                                        gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTAGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                     <  1:109241/140‑1 (MQ=255)
                                                                                          tCGATGATGTCGATTTTTGCGTTGGCAGAAAGTCTTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTTTCACAAGATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                   <  1:2298507/140‑1 (MQ=255)
                                                                                          tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                   <  2:4700000/140‑1 (MQ=255)
                                                                                           cGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTc                                                  >  1:1797107/1‑140 (MQ=255)
                                                                                           cGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTc                                                  >  1:48376/1‑140 (MQ=255)
                                                                                           cGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTc                                                  >  1:5918735/1‑140 (MQ=255)
                                                                                               gatgTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCatat                                              <  2:7444800/140‑1 (MQ=255)
                                                                                                atgTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCatata                                             >  2:5667857/1‑140 (MQ=255)
                                                                                                  gTCGATTTTTGCGTAGGCCGACAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAAATAGTTATTCATATAtg                                           <  2:3744291/140‑1 (MQ=255)
                                                                                                  gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                           <  1:158306/140‑1 (MQ=255)
                                                                                                   tCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtgt                                          >  2:3178922/1‑140 (MQ=255)
                                                                                                     gATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                         >  2:5081673/1‑91 (MQ=255)
                                                                                                       tttttGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTc                                      <  1:987266/140‑1 (MQ=255)
                                                                                                           tGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCcaca                                  >  1:5120664/1‑140 (MQ=255)
                                                                                                            gCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGCTATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACaa                                 <  2:7254439/140‑1 (MQ=255)
                                                                                                               tAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATtg                              <  2:7163308/140‑1 (MQ=255)
                                                                                                                 ggCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGt                                                                                                   >  1:3099521/1‑69 (MQ=255)
                                                                                                                 ggCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATtgtg                            <  1:3607155/140‑1 (MQ=255)
                                                                                                                 ggCCGAAAGTCGGTTCGTGTATTCTTCAATGCGTTGCTTGAGGTCTTTTTCATTTAGTTTTCCGAGTGTCCCAATATTGATATTCACAGGTCATCCCCCCTGTAAAAACAAGTTATTCATATATGTTGTCCACAATtgtg                            >  1:2810669/1‑140 (MQ=255)
                                                                                                                      aaaCTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTAACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGatat                       <  2:7541031/140‑1 (MQ=255)
                                                                                                                            gTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTc                 >  2:3935906/1‑140 (MQ=255)
                                                                                                                             tttCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTATTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCACAACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCt                >  1:5374817/1‑140 (MQ=255)
                                                                                                                                  tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt           <  1:4569038/140‑1 (MQ=255)
                                                                                                                                  tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt           <  2:297669/140‑1 (MQ=255)
                                                                                                                                  tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt           <  2:821377/140‑1 (MQ=255)
                                                                                                                                  tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt           <  1:857430/140‑1 (MQ=255)
                                                                                                                                  tgaaTTCTTCAATGCCTTGCGTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt           <  1:4305061/137‑1 (MQ=255)
                                                                                                                                           cAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAg  >  1:5266083/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAG  >  NZ_CP034943/3976006‑3976284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: