Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,976,149 G→T 7.1% G19G (GGC→GGA rlmH ← 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,976,1490GT 7.1% 1343.2 / 23.8 513G19G (GGC→GGArlmH23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH
Reads supporting (aligned to +/- strand):  ref base G (194/266);  new base T (50/2);  total (245/268)
Fisher's exact test for biased strand distribution p-value = 2.16e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 516 total aligned reads displayed.

TGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGATC  >  NZ_CP034943/3976010‑3976287
                                                                                                                                           |                                                                                                                                          
tGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGTTTCGTGTATTATTAAATg                                                                                                                                            >  2:251719/1‑140 (MQ=255)
tGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATg                                                                                                                                            >  2:1659961/1‑140 (MQ=255)
      ggATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTATGCCGAAAGTCGTTTCGTGTATTCTTAAATTCcttgc                                                                                                                                      >  2:1897047/1‑140 (MQ=255)
      ggATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCGTAGGTTTTCCGGCGCGTTTTAGTCCGGGCGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGGTTGGTGTTTTCTTAAAATCcttgg                                                                                                                                      >  1:2948322/1‑139 (MQ=255)
      ggATGCGGTCGCCGTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCGTAGGTTTTGCGGCGCGTTTTCGTCCGGGGGTTGGGTGATGTAGATTTTTTCGTATGCCGAAAGTGGTTTGGTGTTTTATTGAATGCctggg                                                                                                                                      >  1:3186424/1‑137 (MQ=255)
       gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgct                                                                                                                                     >  2:6971451/1‑140 (MQ=255)
       gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCGTTGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGGGTATGCCGAAAGTGGGTTCGGTTATTATTAAATTCcatggt                                                                                                                                     >  1:3929989/1‑138 (MQ=255)
            ggTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCTTGCTTGAgg                                                                                                                                >  2:667529/1‑140 (MQ=255)
                 ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTATGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCGATTATGTCGATTTTTTCGTAGGCCGAAAGTCGTTTAGTGTATTATTCAAATCCTTTCTTTAGGTAttt                                                                                                                           >  1:955700/1‑140 (MQ=255)
                 ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAAAGCCTTGCGTGAGGTAttt                                                                                                                           >  2:6721178/1‑140 (MQ=255)
                 ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGGTTCGTGTATTCTTCAATGCCTTGCTGGAGGTAttt                                                                                                                           >  1:5657168/1‑140 (MQ=255)
                 ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCGTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCGTGCTTGAGGTAttt                                                                                                                           >  2:6540748/1‑140 (MQ=255)
                        tGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTGGTACTGGAGATATATGATGTAGATTTTTTCGGAGGCCGAAAGTCGGTTAGTTTATTATTCAAATAATTGCTTGAAGTAATTTTCtttt                                                                                                                    >  1:7410144/1‑140 (MQ=255)
                          tCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTAAATTCCTTGCTTGAGGTATTTTTCTTTTat                                                                                                                  >  2:3451034/1‑139 (MQ=255)
                          tCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTAGTCCGGGAGTTCGAAGATGTCGATTTTTTCGTAGGCCCAAACTCCTTTCGTGTATTCTTCAGTGCCTTGCTTGAGGTATTTTTCATTTaa                                                                                                                  >  2:1143956/1‑139 (MQ=255)
                            ttttATGATTTTCATGTCCTGGTCGCGTAGGTTTTCCGGCGCTTTTTCGTACGGGAGTTCCATGATGTCGATTTTTGCGTAGGCCGAAACTCGTTTAGTGTATTCTTAAATTACTTGCTTGAGGTATTTTTATTTTAGtt                                                                                                                >  2:3204543/1‑140 (MQ=255)
                               tATGATTTTCATGTCCTGGTCTCTTAGGTTTTCCCGCGCTTTTTCGTACGGGAGTTCGATGATGTAGCTTTTTGAGTAGGGCCCAAGTCGTTTCGTGTATTCTTCCATGCAGTGCTTGGGGTCTTTTTGGTTTatgtgtg                                                                                                             >  1:6030389/1‑134 (MQ=255)
                               tATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTACGGGAGTTAGATGATTTAGATTTTTGAGTAGGCCGAAAATAGTTTAGTGTATTATTAAATTCCTTTCTTTATGTATTTTTATTTTAGtttta                                                                                                             >  1:2724069/1‑139 (MQ=255)
                               tATGATTTTCATGTCCTGGTCGCGTATGTTTTACGGCGCTTTTTGGTGCGGGAGTTGGATGATGTGGATTTTTTCGTAGGCCGAAAGTCGTTTAGGTTATTAATAAATTACGTTGTTGGAGTATTTTTATTTTATttttg                                                                                                             >  1:3886998/1‑139 (MQ=255)
                               tATGATTTTCATGTCCTGGTCGCGTAGGTTTTACGGCGCTTTTTCGTGCGGGAGTTCGATGTTGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATTCCGTGCTTGAGGTATTTTTGTTTTATtttta                                                                                                             >  1:4609865/1‑139 (MQ=255)
                                   aTTTTCATGTCCTGGTCGCTTAGGTTTTGCGGCTCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                                                         <  1:7220782/140‑1 (MQ=255)
                                   aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                                                         <  2:2387823/140‑1 (MQ=255)
                                   aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                                                         <  1:4088116/140‑1 (MQ=255)
                                   aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                                                         <  2:1289967/140‑1 (MQ=255)
                                   aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCAATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                                                         <  1:6748329/140‑1 (MQ=255)
                                   aTTGTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                                                         <  1:4166346/140‑1 (MQ=255)
                                      ttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGGTTCGTGTATTCTTCAATGCCTTGGTTGAGGTATTTTTCTTTTAGTTTTCCGATTGt                                                                                                      >  1:3363715/1‑140 (MQ=255)
                                      ttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTATGCCGAAAGTCGTTTCGTGTATTCTTCAAAGCCTTGCTTGAGGTAGTTTTATTTTAGTTTTCAGATTGt                                                                                                      >  2:5990239/1‑140 (MQ=255)
                                      ttCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTGGTTGGCCGAAAGTCGTTTGGGGTATTCTTCAATGCCGTGGGTGAGGTATTTTTATTTTTTTTTTGCGAtttt                                                                                                      >  1:217246/1‑138 (MQ=255)
                                      ttCATGTCCTGGTCGCGTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTCGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGt                                                                                                      <  2:890309/140‑1 (MQ=255)
                                      gtCAGGTCCTGGTCGCTTAGGTTGTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTGTCCGATTGt                                                                                                      <  2:3976094/139‑1 (MQ=255)
                                        cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                    <  1:4379567/140‑1 (MQ=255)
                                        cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                    <  1:4975531/140‑1 (MQ=255)
                                        cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                    <  1:3648478/140‑1 (MQ=255)
                                        cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTGTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                    <  1:6184795/140‑1 (MQ=255)
                                                 gTCGCTTAGGTTTTGCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTAAATTCCTTGGTTGAGGTATTTTTATTTTATTTTTAAGATTTTAACAATAttta                                                                                           >  2:635578/1‑138 (MQ=255)
                                                 gTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTAAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTACGATTGTCACAATATTGa                                                                                           >  2:4947236/1‑140 (MQ=255)
                                                   cGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGata                                                                                         <  1:1445437/140‑1 (MQ=255)
                                                   cGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTAAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTACGATTGTCAAAATATTtata                                                                                         >  2:3019886/1‑140 (MQ=255)
                                                      ttAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTGGTGTATTCTTCAATTCCTTGCTTGAGGTATTTTTCTTTTATTTTTCCGATTGTCACAATATTGTTAtta                                                                                      >  2:3597830/1‑139 (MQ=255)
                                                      gtAGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGTTTGGTGTATTATTCAATTCCTTGCTTGAGGTATTTTTATTTTAGTTTTACGATTTTCACAATATTTATAtta                                                                                      >  2:5166128/2‑139 (MQ=255)
                                                       tAGGTTTTGCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTTTCACAATATTGATATTca                                                                                     >  2:2703049/1‑140 (MQ=255)
                                                       tAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTca                                                                                     <  1:2477387/140‑1 (MQ=255)
                                                       tAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTca                                                                                     <  1:5850074/140‑1 (MQ=255)
                                                         ggTTTTCCGGCGCGTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTcaca                                                                                   >  2:3729419/1‑140 (MQ=255)
                                                              tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                                                              >  1:4845854/1‑140 (MQ=255)
                                                              tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                                                              >  1:3479282/1‑140 (MQ=255)
                                                              tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTGGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                                                              >  1:5063769/1‑140 (MQ=255)
                                                              tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTAGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                                                              >  1:6914052/1‑140 (MQ=255)
                                                                        tttCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACttt                                                                    >  1:3398495/1‑140 (MQ=255)
                                                                                 ggAGTTCGATGATGTCGTTTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAg                                                           <  2:4335889/140‑1 (MQ=255)
                                                                                 ggAGTTCGATGATGTCGATTTTTGCGTAGGCCTATAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAg                                                           <  2:7634668/140‑1 (MQ=255)
                                                                                 ggAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAg                                                           <  1:5342137/140‑1 (MQ=255)
                                                                                   aGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGtt                                                         <  1:4934611/140‑1 (MQ=255)
                                                                                    gTTCGATTATGTAGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCTATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGTTTTTCACAATATTGATATTCACATGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:5139328/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:4727229/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:5417282/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:3033808/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:6844589/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:1343049/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:1189235/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:1008926/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTAGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:109241/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTAGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:5305929/140‑1 (MQ=255)
                                                                                      tCGATGATGTCGATTTTTGCGTTGGCAGAAAGTCTTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTTTCACAAGATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      <  1:2298507/140‑1 (MQ=255)
                                                                                      tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      <  2:5920807/140‑1 (MQ=255)
                                                                                      tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      <  1:6565958/140‑1 (MQ=255)
                                                                                           gatgTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCatat                                                 <  2:7444800/140‑1 (MQ=255)
                                                                                           gatgTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCatat                                                 <  2:2121532/140‑1 (MQ=255)
                                                                                            atgTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCatata                                                >  2:3292788/1‑140 (MQ=255)
                                                                                              ttCGATTTTTGCGTAGGCCTAAAGTCGTTTCGTGTATACTACAATGACTTGCTTGAGGTATTTTTCTTTGAGTTTTCCGATTGTCACAATATTGATAGTCACAGGTCATCCGCACTTTAAAAACAAGTTATTCATATAtg                                              <  1:1744236/139‑1 (MQ=255)
                                                                                              gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  1:780408/140‑1 (MQ=255)
                                                                                              gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  1:7283672/140‑1 (MQ=255)
                                                                                              gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTCTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  1:1537566/140‑1 (MQ=255)
                                                                                               tCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATGTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtgt                                             >  2:7120780/1‑140 (MQ=255)
                                                                                                     tttGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCca                                       <  2:2185546/140‑1 (MQ=255)
                                                                                                       tGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCcaca                                     >  2:5555990/1‑140 (MQ=255)
                                                                                                        gCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGCTATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACaa                                    <  2:7254439/140‑1 (MQ=255)
                                                                                                        gCGTAGGCAGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGATGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACaa                                    <  2:5070430/140‑1 (MQ=255)
                                                                                                           tAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      >  2:5767072/1‑119 (MQ=255)
                                                                                                             ggCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGt                                                                                                      <  2:3099521/69‑1 (MQ=255)
                                                                                                             ggCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATtgtg                               <  1:7098161/140‑1 (MQ=255)
                                                                                                             ggCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATtgtg                               <  1:4284905/140‑1 (MQ=255)
                                                                                                             ggCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATtgtg                               <  1:6390805/140‑1 (MQ=255)
                                                                                                              gCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTgg                              >  2:7155597/1‑140 (MQ=255)
                                                                                                                cGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGat                            <  2:2217380/140‑1 (MQ=255)
                                                                                                                  aaaGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGatat                          <  1:5743051/140‑1 (MQ=255)
                                                                                                                  aaaGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGatat                          <  1:2834304/140‑1 (MQ=255)
                                                                                                                  aaaCTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTAACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGatat                          <  2:7541031/140‑1 (MQ=255)
                                                                                                                        gTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTc                    >  1:988280/1‑140 (MQ=255)
                                                                                                                        gTTTCGTGTATTCTTAAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATTTTGTCCACAATTGTGGATATCTTTTc                    >  1:7010550/1‑140 (MQ=255)
                                                                                                                              tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  1:4725303/140‑1 (MQ=255)
                                                                                                                              tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  2:38394/140‑1 (MQ=255)
                                                                                                                              tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  1:6816790/140‑1 (MQ=255)
                                                                                                                              tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  2:2948322/140‑1 (MQ=255)
                                                                                                                              tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  2:821377/140‑1 (MQ=255)
                                                                                                                              tgtAGTCTGCATTGCCTTGCTTGAGGTATTTTTCTTTTAGTGTTCCGATTGTCACAATATTTATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  2:4554570/140‑1 (MQ=255)
                                                                                                                                tATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtttt            >  1:3275522/1‑140 (MQ=255)
                                                                                                                                   tcttcAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTtgtg         <  2:1430474/140‑1 (MQ=255)
                                                                                                                                   tcttcAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTtgtg         <  2:4224824/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGATC  >  NZ_CP034943/3976010‑3976287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: