Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,996,857 C→A 7.2% intergenic (‑163/+43) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,996,8570CA 7.2% 979.9 / 12.3 367intergenic (‑163/+43)purA/EO946_RS20530adenylosuccinate synthase/VOC family protein
Reads supporting (aligned to +/- strand):  ref base C (183/144);  new base A (2/37);  total (185/182)
Fisher's exact test for biased strand distribution p-value = 1.63e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 392 total aligned reads displayed.

TCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCT  >  NZ_CP034943/3996718‑3996994
                                                                                                                                           |                                                                                                                                         
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  1:7546545/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  1:4639364/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  2:1843606/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  1:3549817/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  2:4581575/1‑140 (MQ=255)
       gATTCTATTAAAACAAATCAAGTTTATCAGTTAAGAAACATATAGTTACTTGTAAATCAGTACATTATATTAATTTAAATAATTTTTCGTTATCTTCAATTATTTTTTCTAGATTTTTATCATATCTTTGTCCTAAATAg                                                                                                                                    <  2:7573299/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  1:7428254/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  2:6029124/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             >  2:123325/1‑140 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  2:3186601/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  1:2996061/140‑1 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                        >  1:6371999/1‑140 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                        >  2:3360614/1‑140 (MQ=255)
                     aaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtttt                                                                                                                      <  1:4290954/140‑1 (MQ=255)
                     aaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCAGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtttt                                                                                                                      <  1:2201229/140‑1 (MQ=255)
                      aaTCAAGATTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCCTTGTCCTAAAAAGCTCGTGAAACTTTTa                                                                                                                     >  2:5592162/1‑140 (MQ=255)
                        tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAAGTTTTATc                                                                                                                   >  2:4016158/1‑140 (MQ=255)
                        tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                                   >  2:1056568/1‑140 (MQ=255)
                               tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCTCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTATATCCTTTAGc                                                                                                            >  2:5106148/1‑140 (MQ=255)
                               tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                                            <  2:6845270/140‑1 (MQ=255)
                                aTCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCa                                                                                                           <  2:2375549/140‑1 (MQ=255)
                                 tCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCAc                                                                                                          >  1:6733695/1‑140 (MQ=255)
                                     tGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaa                                                                                                      <  1:7313315/140‑1 (MQ=255)
                                     tGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGCAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaa                                                                                                      <  1:2436989/140‑1 (MQ=255)
                                             cAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTa                                                                                              <  2:230461/140‑1 (MQ=255)
                                               gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCTTTCGCTTCCATTCGATTTACAAGCGTTTTCACATCGCATTGTCCTAAAAAGCTCGTGAAACTATAATCCTTTAGCACGAAAAAGAGTTACt                                                                                            >  1:6230342/1‑140 (MQ=255)
                                               gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                            >  1:7622167/1‑140 (MQ=255)
                                               gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                            <  2:3634045/140‑1 (MQ=255)
                                               gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                            <  2:7372933/140‑1 (MQ=255)
                                                aaaGTCAATTGTAAATCCGACCATTAAATTCATGTAAATCAATATTCGTTCGCGTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGAGAAACTTTTATCCTTTAGCACGAAAAAGAGGTACtt                                                                                           <  1:1958538/140‑1 (MQ=255)
                                                  aGTCAATTGTACATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGc                                                                                         <  2:5644651/140‑1 (MQ=255)
                                                      aaTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                     <  1:7204746/140‑1 (MQ=255)
                                                      aaTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                     <  1:1068630/140‑1 (MQ=255)
                                                      aaTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                     <  1:444399/140‑1 (MQ=255)
                                                       aTTGTAAATCCGAACATTAAATTAATGTAAATCAATAGTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGat                                                                                    <  1:5199904/140‑1 (MQ=255)
                                                        ttGTAAATCCGAACATTAAATTACTGTAAATCAATATCCGGTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                                   <  1:4834178/140‑1 (MQ=255)
                                                        ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                                   >  2:667345/1‑140 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                  <  1:6317261/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                  <  2:4932203/140‑1 (MQ=255)
                                                          gTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACACGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATc                                                                                 >  2:154553/1‑140 (MQ=255)
                                                             aaTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCt                                                                              >  2:5261578/1‑140 (MQ=255)
                                                              aTCCGAACATTAAATTAATGTAAATCTATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTACTAAAAAGCTCGTGAAACTTTTATCCTTGAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                                             <  2:1898669/140‑1 (MQ=255)
                                                              aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                                             >  2:4049335/1‑140 (MQ=255)
                                                              aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGAACGAAAAAGAGTTACTTGCCTGATATCCAtt                                                                             >  2:2750408/1‑140 (MQ=255)
                                                              aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTGTAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                                             <  2:2509071/140‑1 (MQ=255)
                                                                   atcATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTGGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        <  2:7135531/138‑1 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        <  1:395160/140‑1 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        <  2:6172395/140‑1 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        <  1:420440/140‑1 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        >  1:606199/1‑140 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        >  1:5491499/1‑140 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        >  1:5667264/1‑140 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTATCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        >  1:6528442/1‑140 (MQ=255)
                                                                        taaattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCAt                                                                   >  1:5440877/1‑140 (MQ=255)
                                                                               aTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACg                                                            >  1:325215/1‑140 (MQ=255)
                                                                                tttAAATAACTATTGGTTCGCTTCAATTCTTTTTACATGGGTTTTCTATTCGAATTTTCCTAAAACCCTCGTGAAACTTTTATCATTTCCCACTAAAATGTTTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGa                                                           <  2:3771790/138‑1 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCCTTGTCATAAAAAGCTCGTGAAACTTTTATCCTTTAGCAGGAAATAGAGTTACTTGCCTGATATCCGTTTTGTATTGATAAAAACGa                                                           >  1:6254654/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTATCACGAAAAAGAGTTACTTGACTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  2:4642775/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAActa                                                           >  2:7000597/1‑138 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:1017050/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:1193425/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:6834630/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:5564771/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCGGATATCCCTTTTGTATTCATAAAAACGa                                                           >  2:6261687/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGATCGTGAAAATTTTATCCTTTAGCACTAAAAAAAGTTAATAGCATGATATCCCTTTTGTATTCATAAAAActa                                                           >  2:5812413/1‑138 (MQ=255)
                                                                                     aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGc                                                      >  2:5082092/1‑140 (MQ=255)
                                                                                     aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGc                                                      >  2:4325154/1‑140 (MQ=255)
                                                                                     aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGc                                                      >  2:1374797/1‑140 (MQ=255)
                                                                                     aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGc                                                      >  2:2217546/1‑140 (MQ=255)
                                                                                             tggttcgCTTCATTTCGTTTTACAAGCGTTTTCTCATCGATTTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCATGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTc                                              <  2:6567091/138‑1 (MQ=255)
                                                                                             tcgttggCTTCAATTGTTTTTACATGCTTTTTCTCATCGATTTGTCTTAAAAAGGTTGTTAAATTTTTTTAGTTTCTCACGAAAAAGAGTATCTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTc                                              <  2:4112083/140‑1 (MQ=255)
                                                                                             tcgttcgCTGCCATTCGTTTTACACGCGTTTTCTCAACGCATTGTCATAAAAACCTCGTTAAACTTTTATCCTTTACCACCAAAAAGAGTTACTTGCCTGATATCCATTTTGTATACATAAAAACGACTCGCCAACCGTc                                              <  2:3097035/140‑1 (MQ=255)
                                                                                              cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTcc                                             >  2:296965/1‑140 (MQ=255)
                                                                                               gttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATACTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTcag                                            >  2:4788526/1‑138 (MQ=255)
                                                                                                ttcgCTTCCATTCGTTTTAAAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTCAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATAGCCCTTTTTTATTCATAAACACGACTCGCCAACCGTCCgg                                           <  2:5931240/140‑1 (MQ=255)
                                                                                                tgggtttCCCTTCCTTTTACCAGCGTTGTCTCAGCGCATTGGCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCGCATATCCGTGTTGTATTCAGAAAAACGACTCGCCAACCGTCCgg                                           <  2:4377483/135‑1 (MQ=255)
                                                                                                tgcgCTTCCTTGGGTTTTACACGCGTTTGCTCATCGCATGGTCCTAAAACGCTCGGGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTGGCCTGATATCGCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCgg                                           <  2:389364/138‑1 (MQ=255)
                                                                                                 tcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGa                                          >  2:2996013/1‑140 (MQ=255)
                                                                                                 tcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCAGAAAAAAGACTCGCCAACCGTCCGGa                                          >  1:3031498/1‑140 (MQ=255)
                                                                                                    cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTCTTCATAAAAACGACTCGCCAACCGTCCGGATcc                                       >  2:6758894/1‑140 (MQ=255)
                                                                                                    cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                       >  1:6655665/1‑140 (MQ=255)
                                                                                                    cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                       >  2:6525281/1‑140 (MQ=255)
                                                                                                         atgggTTTTAAAAGCTTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTAt                                  <  2:5491499/136‑1 (MQ=255)
                                                                                                            ttttttACATGCGTTTTCTCATCGATTTTTAATAAAAAGCTGGTTAAAATTTTATCCTTTAGCACCAAAAAGAGTTAGTTGCCTTATATCCCTTTTTTATACATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGt                               <  1:7653556/138‑1 (MQ=255)
                                                                                                            ctttttACAAGCTTTTTCTCATCGCATTGTCATAAAAAGCTCTTTAAACTTTTATCCTTTAGCACGAAAAAGATTTACTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGt                               <  2:5328338/138‑1 (MQ=255)
                                                                                                              ttttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                             >  2:163884/1‑140 (MQ=255)
                                                                                                              ttttAAAAGGTTTTTATAATCGATTTTTCAAAAAAAGATGTTTAAATTTTTATCCTTTAGCACCAAAATTTTTTAGTTGCATTATATCCTTTTTTTTTTAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                             <  1:1521859/140‑1 (MQ=255)
                                                                                                                  aCAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGCGAAACTTTTATCCTTTAGCACGAAAACGTGTTACTTGCCTTATATCCCTTTGGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGcc                         <  2:3524074/140‑1 (MQ=255)
                                                                                                                      gggTTTTCGAATCGCTTTGTCCAAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTAGTTGCCGGATATCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCCTCACGCCTCCa                     <  2:2025674/138‑1 (MQ=255)
                                                                                                                       gtttttCTCTGCGCTTTTTCAAAAAAAGCTGTTCAAAATTTTAACCTTACCCCCCAAAATGTTTTCCTGGCCTTAAAACACTTTTGTAATCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  1:3818328/138‑1 (MQ=255)
                                                                                                                       ggTTTTCTCATCGCTTTGTCCTAAAACGCTCGTCAAATTTTTATCCTTTAGCACCAAAAAGTGTTAGTGGCCTGATATCCCTTTTTTATTAATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  1:6920753/139‑1 (MQ=255)
                                                                                                                         ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                  >  1:3727396/1‑140 (MQ=255)
                                                                                                                         ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                  >  1:4060782/1‑140 (MQ=255)
                                                                                                                         ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                  >  1:2704020/1‑140 (MQ=255)
                                                                                                                          tttCTCATCGCATTGTCCTAAAAAGCACGTTAAACTTTTAACCTTTCGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTTGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                 <  1:2831569/140‑1 (MQ=255)
                                                                                                                             atcAGCGCATTGTCCAAAAAAGCTCGTGAAACTTTTATCCTTTCGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTcc              <  2:2647338/139‑1 (MQ=255)
                                                                                                                                 tCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGc                                                                                         >  2:6430079/1‑61 (MQ=255)
                                                                                                                                    taTTGTCAAAAACAGCTGTTCAAACTTTTATCCTTGACCACCAAAAAGAGTTAGTTGCCTGATATCCCTTTTGTATTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACgg       <  1:1246951/139‑1 (MQ=255)
                                                                                                                                     aTTGTCAAAAAAAGCTCGTGAAACTTTTATCCTTTACCACCAAAAAGAGTTACTTGCCTGATATCCCTTTTTTATACAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACggg      <  1:1707706/140‑1 (MQ=255)
                                                                                                                                         tCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACCAAAAAGAGTTAGTTGCCTGTTAGCCGTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct  <  1:1843606/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCT  >  NZ_CP034943/3996718‑3996994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: