Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,996,891 G→T 5.8% intergenic (‑197/+9) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,996,8910GT 5.8% 1338.8 / 4.8 541intergenic (‑197/+9)purA/EO946_RS20530adenylosuccinate synthase/VOC family protein
Reads supporting (aligned to +/- strand):  ref base G (299/148);  new base T (2/47);  total (301/240)
Fisher's exact test for biased strand distribution p-value = 1.07e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 547 total aligned reads displayed.

TGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCT  >  NZ_CP034943/3996755‑3997030
                                                                                                                                         |                                                                                                                                           
 gaagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaa                                                                                                                                         <  1:5600369/140‑1 (MQ=255)
  aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaaa                                                                                                                                        >  2:2724351/1‑140 (MQ=255)
     aaaCAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCATAAAAACCTCGTGAAACTTTTATCCTTTAGCAAGAAAAatag                                                                                                                                     >  2:7457954/1‑140 (MQ=255)
        cAGAAAGTCAAGTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTa                                                                                                                                  <  1:76442/140‑1 (MQ=255)
           aaaGTCAATTGTAAATCCGACCATTAAATTCATGTAAATCAATATTCGTTCGCGTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGAGAAACTTTTATCCTTTAGCACGAAAAAGAGGTACtt                                                                                                                               <  1:1958538/140‑1 (MQ=255)
           aaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                                                                               <  1:2641099/140‑1 (MQ=255)
             aGTCAATTGTACATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGc                                                                                                                             <  2:5644651/140‑1 (MQ=255)
             aGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGc                                                                                                                             <  2:3517766/140‑1 (MQ=255)
                 aaTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                                                         <  2:2327809/140‑1 (MQ=255)
                 aaTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                                                         <  1:158382/140‑1 (MQ=255)
                   ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                                                                       <  1:546922/140‑1 (MQ=255)
                    tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                                                      <  2:2475281/140‑1 (MQ=255)
                              atcATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTGGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                                                            <  2:7135531/138‑1 (MQ=255)
                              aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                                                            >  1:606199/1‑140 (MQ=255)
                              aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                                                            >  1:4326240/1‑140 (MQ=255)
                                           tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                               >  2:5962968/1‑140 (MQ=255)
                                           tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTATCACGAAAAAGAGTTACTTGACTGATATCCCTTTTGTATTCATAAAAACGa                                                                                               >  2:4642775/1‑140 (MQ=255)
                                           tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAActa                                                                                               >  2:7000597/1‑138 (MQ=255)
                                           tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                               >  2:3625161/1‑140 (MQ=255)
                                           tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                               >  1:7247452/1‑140 (MQ=255)
                                           tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                               >  1:1017050/1‑140 (MQ=255)
                                           tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                               >  1:1748609/1‑140 (MQ=255)
                                             tAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACt                                                                                             >  2:1246951/1‑140 (MQ=255)
                                             tAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACt                                                                                             >  2:2646703/1‑140 (MQ=255)
                                               aaTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCg                                                                                           >  2:653659/1‑140 (MQ=255)
                                                aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCCCGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACTACTCGc                                                                                          >  2:4324495/1‑140 (MQ=255)
                                                     tatTCGGTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAACACGACTCGCCAAcc                                                                                     >  1:5023222/1‑140 (MQ=255)
                                                     tactcgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTGTTGTATTCATAAAAACGACTCGCCAAcc                                                                                     >  2:2240547/4‑140 (MQ=255)
                                                        tcgttggCTTCAATTGTTTTTACATGCTTTTTCTCATCGATTTGTCTTAAAAAGGTTGTTAAATTTTTTTAGTTTCTCACGAAAAAGAGTATCTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTc                                                                                  <  2:4112083/140‑1 (MQ=255)
                                                        tcgttcgCTGCCATTCGTTTTACACGCGTTTTCTCAACGCATTGTCATAAAAACCTCGTTAAACTTTTATCCTTTACCACCAAAAAGAGTTACTTGCCTGATATCCATTTTGTATACATAAAAACGACTCGCCAACCGTc                                                                                  <  2:3097035/140‑1 (MQ=255)
                                                            tcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGa                                                                              >  2:2996013/1‑140 (MQ=255)
                                                                    atgggTTTTAAAAGCTTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTAt                                                                      <  2:5491499/136‑1 (MQ=255)
                                                                          tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                                                                >  1:4065894/1‑140 (MQ=255)
                                                                          tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTGTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                                                                >  1:6675945/1‑140 (MQ=255)
                                                                                 gggTTTTCGAATCGCTTTGTCCAAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTAGTTGCCGGATATCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCCTCACGCCTCCa                                                         <  2:2025674/138‑1 (MQ=255)
                                                                                  gtttttCTCTGCGCTTTTTCAAAAAAAGCTGTTCAAAATTTTAACCTTACCCCCCAAAATGTTTTCCTGGCCTTAAAACACTTTTGTAATCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                        <  1:3818328/138‑1 (MQ=255)
                                                                                  ctttttCACATCTCATTGTCATAAAAAGCTCTTGAAACTTTTACCCTTTCGCACTAAAAATCGTTAGTTGCCTGATAGCCCTTTTGTATAAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                        <  2:4500431/138‑1 (MQ=255)
                                                                                    ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                                                      >  2:5194907/1‑140 (MQ=255)
                                                                                    ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                                                      >  2:3851476/1‑140 (MQ=255)
                                                                                    ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                                                      >  1:2347544/1‑140 (MQ=255)
                                                                                       gctctTCGCTTTGTCATAAAAACCTGTCAAAATTTTTATCCTTACGCACCAAAACTGTTTAGTTGCCTGATATCCTTTTGGTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTc                                                   <  1:4392149/139‑1 (MQ=255)
                                                                                            tCGCTTTGTCCTAAAAAGATCTTCAAACTTTTAACCTTTCGCACGAAAAAGTGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa                                              <  1:1855719/140‑1 (MQ=255)
                                                                                             cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc                                             >  2:478407/1‑140 (MQ=255)
                                                                                             cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc                                             >  1:6262582/1‑140 (MQ=255)
                                                                                             cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc                                             >  1:2804282/1‑140 (MQ=255)
                                                                                                ttggTCATAAACCGCTCGTTATACTTTAATCCTTTACCCCCAAACAGAGTTCGGTGCCTGCTATCCCTTTTGTATTCAGAAACACGACTCGCCTACCGTCCGGATCCTCTATCGGCACGCCTCCATGGCTCCAGTACggg                                          <  1:6907332/137‑1 (MQ=255)
                                                                                                aTTGTCCTAAAACGCTCGTTAAATTTTTATCCTTTACCCCCAAAAATAGTTACTGGCCTGATATCCCTTTTTTTTACATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACggg                                          <  2:4030907/140‑1 (MQ=255)
                                                                                                    tCCTAAAAAGCTCGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct                                      <  2:1642524/140‑1 (MQ=255)
                                                                                                           atgCTGGTGAAACTTTTATCCTTTAGCACCAAAAAGTGTTACTTGCCTGATAGCCCTTTTTTTTTCTCAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                               <  1:3354115/138‑1 (MQ=255)
                                                                                                           acgCTCGTGAAACTTTTATCCTTTCCCACTAAAAATTTTTATTTGCCTGATATCACTTTTGTATTAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                               <  1:7573147/138‑1 (MQ=255)
                                                                                                           acgCTCGTGAAACTTTTAACCTTTAGCACCAAAAAGAGTGAGTTGCCTGATATCCCTTTTGTATTAACAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                               <  1:715986/138‑1 (MQ=255)
                                                                                                           aaGCTCGTCAACCTTTTAACCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                               <  2:5069042/140‑1 (MQ=255)
                                                                                                              ctctTGAAAGTTTTAGCCTTTCGCACTAAAAAGAGTTACTTGCCTGATATCCGGTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            <  1:5261578/140‑1 (MQ=255)
                                                                                                              ctctTGAAACTTTTATCCTTTATCACTAAAAATAGTTACTTGCCTGATATCACTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCTTCACGACTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  2:1072728/1‑140 (MQ=255)
                                                                                                              ctctTGAAAATTTTATCCTTGCCCACGAAAAAGAGTTACTGGCCTGATATCCGTTTTTTATAAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            <  1:296965/140‑1 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGCGTTACTTGCCTGATATCCCTTTTGTAGTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTCTCGTCACGCCTCCATGGCTCCAGTACGGGTTGTCAGATTCAAc                            >  1:4867134/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCTTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  1:5865342/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCTGATTCAAc                            >  1:3057183/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  2:3911043/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  2:4915754/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  2:6331153/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  2:82056/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  1:1101695/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  2:6185089/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  2:6087205/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  2:2917284/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  1:1479860/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  1:27549/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  1:4035601/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  1:5376014/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  1:550566/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  1:6355680/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  1:6530657/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  1:6817782/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  2:1532608/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAACGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                            >  1:1221259/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTGTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCCGATTCAAc                            >  1:3574370/1‑140 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGGCCGGATCCTCTCTCGTCACGCCTCCATGGCTCCAGGACGGGTTCTCAGATTCaag                            >  1:4771611/1‑139 (MQ=255)
                                                                                                              cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGACTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGTTTCTCAGATTCAAc                            >  1:3936302/1‑140 (MQ=255)
                                                                                                               tctttaaaTTTTTATCTTTTAGCACTAAAAAGAGTTACTTGCATGATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAcc                           <  2:6839402/135‑1 (MQ=255)
                                                                                                               tctttaaaTTTTTATCCTTTACCACTAAAAAGAGTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAcc                           <  2:6719569/135‑1 (MQ=255)
                                                                                                               tCGTTAAACTTTTATCCTTTAGCACGAAAATGTTTTACTTGCCTTATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAcc                           <  2:1857591/140‑1 (MQ=255)
                                                                                                               tCGTGAAACTTTTATACTTTTGCTCTAAAAATATTTAATTGACTTATATCCGTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGATCCAGTACGGGTTCTCAGATTCAAcc                           <  2:7602848/140‑1 (MQ=255)
                                                                                                                 ggtgaaCTTTTTTCCGTTTCCCACGAAAAATTTTTAGTTGCCTGATACCCCTTTTTTTTAAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACct                          <  1:4581575/136‑1 (MQ=255)
                                                                                                                     caCTTTTAGCATTTACCACGAAAAAGTGTTCGTTGCCTGATTTCCATTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                     <  2:5390299/139‑1 (MQ=255)
                                                                                                                     aaattttATCCTTTAGCACCAAAAAGAGTTACTTGCCTTATTTCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                     <  1:1330130/137‑1 (MQ=255)
                                                                                                                     aaatttaATCCTTAAGCACGAAAAAGATTTACTTGCCTGATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                     <  1:7449345/137‑1 (MQ=255)
                                                                                                                     aaatttaATCATTTCGCACTAAAACGAGTTACTTGCCTGTTAGCCGTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAGGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                     <  2:3716257/137‑1 (MQ=255)
                                                                                                                        ttttATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAg                  >  1:7316740/1‑140 (MQ=255)
                                                                                                                           tATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                        >  1:902535/1‑99 (MQ=255)
                                                                                                                           tATCCTTTAGCACGAAAAAGAGTTACTGGCCTGATATCCATTTGGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGccc               <  2:6655665/140‑1 (MQ=255)
                                                                                                                            aTCCTTTCGCACCAAAATTTGGTAGTGGCCTGATATCCGTTTTCTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGGACGGGTTCTCAGATTCAACCTCTTGATAGCCCa              <  1:368791/140‑1 (MQ=255)
                                                                                                                            aTCCTTTACCACTAAAAAGAGTTACTTGCCTTATATCCCTTTTTTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCa              <  2:1479860/140‑1 (MQ=255)
                                                                                                                            aTCCTTTACCACGAAAAATATTTACTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCa              <  2:5893392/140‑1 (MQ=255)
                                                                                                                                   tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt        >  1:6230180/1‑140 (MQ=255)
                                                                                                                                    accACTAAAAAGAGTTAATTGCCTGATATCCCTTTTGTCTTCATAAAAACGACTCGCCAACCGGCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt       <  1:5646886/138‑1 (MQ=255)
                                                                                                                                    aGCACGAAAACGTGTTACTTGCCTGATCTCCCTCTGGTCTGCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt       <  2:4396912/140‑1 (MQ=255)
                                                                                                                                    aGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt       <  2:90928/140‑1 (MQ=255)
                                                                                                                                         gAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCt  <  2:325215/140‑1 (MQ=255)
                                                                                                                                         gAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATGCATAAAAACGACTCGCCACCCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCGCGGACGGGGTCTCAGCGTCATGCTCGGGAGCGCGCATGTGTTTTCGCt  >  2:5784482/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCT  >  NZ_CP034943/3996755‑3997030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: