BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	4,000,117	G→T	5.1%	L121F (TTG→TTT)	EO946_RS20555 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	4,000,117	0	G	T	5.1%	2200.5 / 33.0	772	L121F (TTG→TTT)	EO946_RS20555	MFS transporter
Reads supporting (aligned to +/- strand): ref base G (217/492); new base T (59/3); total (277/495)
Fisher's exact test for biased strand distribution p-value = 7.48e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 781 total aligned reads displayed.

CGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGAAGC  >  NZ_CP034943/3999979‑4000256

 ggAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGcc                                                                                                                                           <  2:2332342/140‑1 (MQ=255)

        aGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGGCGCTTTATTTTTTGGAAAGGCACTCATAGGAGTCGGAATTGGGATAGGAAACGGTGTTTTTGCAAGCttt                                                                                                                                    >  1:6665093/1‑139 (MQ=255)

          cGAACCTTATGGGTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCGGGTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAAt                                                                                                                                  <  2:4749940/140‑1 (MQ=255)

          cGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAAt                                                                                                                                  <  2:343372/140‑1 (MQ=255)

            aaCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                                                                <  2:6119133/140‑1 (MQ=255)

              ccTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCaaa                                                                                                                              <  1:2246733/140‑1 (MQ=255)

              ccTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCaaa                                                                                                                              <  1:4187327/140‑1 (MQ=255)

              ccTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCaaa                                                                                                                              <  1:3541369/140‑1 (MQ=255)

                  aTGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGCTTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAAAGGCACACATCGGAGTCGGAATAGCGATCGGAAACGGTGTTTTGCCAAGCTTAAGCAAACATa                                                                                                                          >  2:6879689/1‑140 (MQ=255)

                      cTTGGTCTCCTGATCCTGTTTATCGGCGTGTTGATTCGTTCTGCCGGCTATACAGCCGCTTTATTTTTGGGTAAGGCACACAGCGGAGTAGGCATATCGATCGGAAACCTTGTTTTTCCAAGCTTAATAAAACAGaaaaa                                                                                                                      >  1:5280175/1‑138 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGGTATACTGCCGCTTTATTTTTCGGAACGGGAAACCTCGGGGTGGGTATTGGGATCGGCAACGTTGTTTTTGCAATCGTTATAAAACATaaaaaacc                                                                                                                   >  1:263213/1‑135 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                                                                >  2:5216951/1‑127 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATAacc                                                                                                                   >  2:3902657/1‑140 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACCGCACTCATCGGAGTCGGAAATTCGATAGGAAACGTTCTTTTTCCAAGCTTAATCAAACATAAATAcac                                                                                                                   >  2:6425317/1‑138 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGGTCTGCCGGCTATACTGGCGCTTTTTTTTTTGGGACGGCACTCATGGGAGTCGGCATTGGGATAGGGAACGTTCTTTTTGCAAGCTTAATAAAAAAAAAATAacc                                                                                                                   >  1:611670/1‑140 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGGGTTGATTCGTTCTGCCGGCTATACTGCCGCTGTATTTTTCGGAAAGGCCCACATCGGCGTCGGCAATGCGAACGGAAACGGTCGTTTGCCAACCTTAATAAAACAAAAAAAccc                                                                                                                   >  1:1605062/1‑140 (MQ=255)

                         ggTCTCCTGATCCTGTTAATCGGCGTTTTGATTCGTTATTCCGGCTATACTGCCGCTTTATTTTTCGGAACCGCAATAAAAGCAGTAGGAAATTCGATCGGAAACCTTCTTTTTCCAACATTAAACAAACAaaaaaaaac                                                                                                                   >  2:4292871/1‑135 (MQ=255)

                            ctcCTGATCCTGTTTAGCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGa                                                                                                                <  1:517937/140‑1 (MQ=255)

                             tcCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGaa                                                                                                               <  1:3738093/140‑1 (MQ=255)

                             tcCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGaa                                                                                                               <  2:2983854/140‑1 (MQ=255)

                                  aTCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTc                                                                                                          <  2:2652447/140‑1 (MQ=255)

                                    ccagTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTGTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCACACATAAATCCCCTGAAAAGTCAg                                                                                                        <  1:1487090/137‑1 (MQ=255)

                                    ccTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAg                                                                                                        <  1:3653838/140‑1 (MQ=255)

                                    ccTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAg                                                                                                        <  1:850414/140‑1 (MQ=255)

                                    ccTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAg                                                                                                        <  2:7615238/140‑1 (MQ=255)

                                    ccTGTTTATCGGCGTTTTGATACGTTCTGCCGGCTATACTGCCGCTTTATTTGTCGGAACGGCACTCATCGGAGTCGGAAGTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAAGACCCTGAAAAGTCAg                                                                                                        <  1:3342093/140‑1 (MQ=255)

                                     cTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAgg                                                                                                       >  2:1536300/1‑140 (MQ=255)

                                        tttATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGAt                                                                                                    <  1:6587229/140‑1 (MQ=255)

                                              gtcGTTTTGATTCGTTCTGCCGGCTATTCTGCCTCTTTATTTTTCGGAACGGCACTCATCGGACTCGGAATTGCGATCGGAAACGTTATTTTGACAAGCTTAATCAAACATAAATAACCTGAAAAGACAGGGATCATGAt                                                                                              <  2:7011439/138‑1 (MQ=255)

                                              ggCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                                                                              <  2:3654692/140‑1 (MQ=255)

                                              ggCGTTTTGATTCGTGCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCATTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                                                                              <  1:4933780/140‑1 (MQ=255)

                                              ggCGTTGGGATTGGTTCTGCCGGCTATACTGCCGCTGTATTTTTCGGAACGGGACTCATCGGAGTCGGATTTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                                                                              <  2:5961851/140‑1 (MQ=255)

                                               gCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGCACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                                                                             <  2:6515978/140‑1 (MQ=255)

                                               gCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                                                                             <  1:5721836/140‑1 (MQ=255)

                                                cGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCa                                                                                            <  2:2785979/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTGGGAACGGCAGACATCGGAGTAGGAATTTCGATAGGAAACCTTGTTTTGCCAATCTTAATCAAAAATAAATACCCGGAAAATTCAGGGATCAGGATGAGc                                                                                          >  2:3541453/1‑140 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGGGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:627930/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:7338753/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:2139759/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:4160777/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:1584541/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:444864/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:1113376/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:4445533/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:712563/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:5062327/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:7463984/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:7127625/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:2140664/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:5468793/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:3344595/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:5587644/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACCGCAATCATCGGAGTCGGAATTGCGATCGGAAACGGTCTTTTGCCAAGCTTAATAAAACATAAATAACCGTAAAAGTCAGGGATCATTATAAGc                                                                                          >  2:7291733/1‑140 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATGTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:5788591/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGATTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:7391126/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTACCGGCTATACTGCCGCTTTATTTTTAGGAACGGCACTCATCGGAGTGGGAATTGCGATCGGAAACCGTCTTTTTGCCAGCGTAATAAAACAAAAATAACCAGAAAACTCAGGGATCAAGATAAGc                                                                                          >  2:1999092/1‑140 (MQ=255)

                                                  atttcaatCGTTCTGACGGCTATACTGACTCATAATATTTCGCTACCGCAATCAGCGGAGTCGGAATGGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAAAATAACTCCCCTGAAAACGCAGGGATCATGATCAGc                                                                                          <  1:2467157/133‑1 (MQ=255)

                                                         tCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCTGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGtata                                                                                   <  1:1042440/140‑1 (MQ=255)

                                                         tCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGtata                                                                                   <  1:4099152/140‑1 (MQ=255)

                                                         tCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGtata                                                                                   <  1:7020662/140‑1 (MQ=255)

                                                            ttCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGa                                                                                <  2:2895590/140‑1 (MQ=255)

                                                                 ccGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCGGCCTGTATACGACATCa                                                                           <  2:307588/140‑1 (MQ=255)

                                                                 ccGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                                                                           >  2:6078778/1‑140 (MQ=255)

                                                                 ccGGCTATACTGCCCCTTTATTTTTCGGAACGGCACTCCTCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                                                                           <  2:4591514/140‑1 (MQ=255)

                                                                 ccGGCTAGACTGCCGCTTGAGTTTTCGGACCGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                                                                           <  2:4235655/140‑1 (MQ=255)

                                                                     cTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGa                                                                       <  2:3179433/140‑1 (MQ=255)

                                                                      tataCTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGaa                                                                      <  1:2757299/140‑1 (MQ=255)

                                                                             ccGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTc                                                               >  2:4874757/1‑140 (MQ=255)

                                                                             ccGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTc                                                               >  2:1895906/1‑140 (MQ=255)

                                                                             ccGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAAACAAAAATAAATAACCTGAAAAGTAAGGGATAATGATAAGCCTGTAAACGACATAAAAGAATAAATTc                                                               >  2:6295770/1‑140 (MQ=255)

                                                                              cGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCg                                                              <  1:1355274/140‑1 (MQ=255)

                                                                              cGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCg                                                              <  1:1752/140‑1 (MQ=255)

                                                                              cGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCGGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCg                                                              >  2:3797981/1‑140 (MQ=255)

                                                                                   tATTTTTCGGCACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAATTCAGGGATCATGATCAGCCTGTAGACGACATCAATGAATATATTCgctgct                                                         <  2:1269220/140‑1 (MQ=255)

                                                                                   tATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCgctgct                                                         <  1:3155511/140‑1 (MQ=255)

                                                                                     tttttCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCttt                                                       <  2:5775677/140‑1 (MQ=255)

                                                                                     tttttCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCttt                                                       <  2:5441984/140‑1 (MQ=255)

                                                                                     ttgttCGGAACGGAACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTTCCAAGCTTAATCAAAGATATATACCCTGAAAAGTCAGGGATCATGATCAGCGTGTATACGACATCAATGAATATATTCGCTGCttt                                                       <  2:5582626/137‑1 (MQ=255)

                                                                                          cGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCAt                                                  <  2:2456117/140‑1 (MQ=255)

                                                                                           ggAACGGCACTCATGGGTTTCGGTATTGCGATCGGAATGGTTCTTTTGCCTAGCTTAATCAAACATAAATACCCTGAAATGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATTTTTTCGCTGATTTGGCATc                                                 <  2:1720658/140‑1 (MQ=255)

                                                                                           ggAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATc                                                 <  2:852943/140‑1 (MQ=255)

                                                                                               cGGCACTCCTCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAggg                                             <  1:118474/140‑2 (MQ=255)

                                                                                               cGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGGTGCTTTGGCATCAGgt                                             <  1:4686393/140‑1 (MQ=255)

                                                                                                     tCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGc                                       <  1:2858204/140‑1 (MQ=255)

                                                                                                       aTCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGACTATATTCGCTGCTTTGGCATCAGGTGTCAGCGt                                     >  1:1886845/1‑140 (MQ=255)

                                                                                                       aTCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGt                                     >  1:4785090/1‑140 (MQ=255)

                                                                                                       aTCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGt                                     >  1:6789119/1‑140 (MQ=255)

                                                                                                         cGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTAc                                   >  1:5395182/1‑140 (MQ=255)

                                                                                                            aGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCCATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACctc                                >  1:6474768/1‑140 (MQ=255)

                                                                                                            aGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACctc                                >  1:3882170/1‑140 (MQ=255)

                                                                                                                 gAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTggcg                           >  1:5917330/1‑140 (MQ=255)

                                                                                                                 gAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTggcg                           >  2:1007916/1‑140 (MQ=255)

                                                                                                                  aaTTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTggcgg                          <  2:5333846/140‑1 (MQ=255)

                                                                                                                  aaTTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTggcgg                          <  1:7292056/140‑1 (MQ=255)

                                                                                                                  aaTTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTggcgg                          <  2:5060579/140‑1 (MQ=255)

                                                                                                                        gATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACaaa                    >  2:2052170/1‑140 (MQ=255)

                                                                                                                                 cGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCg           >  2:6772786/1‑140 (MQ=255)

                                                                                                                                    tCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGAt        <  1:7670781/140‑1 (MQ=255)

                                                                                                                                    tCTTTTGCCAAGCTTAATCAAACATAAAGACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGAt        <  2:6665093/140‑1 (MQ=255)

                                                                                                                                          gCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGaagc  <  1:6883603/140‑1 (MQ=255)

CGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGAAGC  >  NZ_CP034943/3999979‑4000256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`