Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 361,352 T→A 9.3% M147K (ATG→AAG)  EO946_RS01960 → FAD/NAD(P)‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943361,3520TA 9.3% 1841.4 / 40.2 662M147K (ATG→AAG) EO946_RS01960FAD/NAD(P)‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (280/303);  new base A (73/2);  total (357/305)
Fisher's exact test for biased strand distribution p-value = 3.41e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 664 total aligned reads displayed.

GAGACTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATA  >  NZ_CP034943/361213‑361488
                                                                                                                                           |                                                                                                                                        
gagaCTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCAt                                                                                                                                          <  1:829769/140‑1 (MQ=255)
    cTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCCAGAGt                                                                                                                                      >  2:4419511/1‑140 (MQ=255)
    cTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCCTGAGt                                                                                                                                      >  1:4005/1‑140 (MQ=255)
    cTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGt                                                                                                                                      >  2:470462/1‑140 (MQ=255)
       tgCTAGATGAAATACATATGGATCCAGCATGGTAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGGTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGtttt                                                                                                                                   >  1:1927061/1‑140 (MQ=255)
       tgCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGTCAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGtttt                                                                                                                                   >  1:4298484/1‑140 (MQ=255)
       tgCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCAATAGtttt                                                                                                                                   >  1:479980/1‑140 (MQ=255)
       tgCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGtttt                                                                                                                                   >  1:5565984/1‑140 (MQ=255)
       tgCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTATCCATGAGtttt                                                                                                                                   >  2:2365376/1‑140 (MQ=255)
            gATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCtca                                                                                                                              >  1:3794729/1‑140 (MQ=255)
             aTGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCtcat                                                                                                                             <  2:5201595/140‑1 (MQ=255)
               gAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCtcatca                                                                                                                           <  2:3790275/140‑1 (MQ=255)
                 aaTACATATGGATCAAGCATGGAAAACATGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCCAGAGTTTTTGTCATCAGc                                                                                                                         >  2:5782530/1‑140 (MQ=255)
                 aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTTGCCATGAGTTTTTGTCATAAGc                                                                                                                         >  2:1770752/1‑140 (MQ=255)
                 aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCATGAGTTTTTCtcagaacc                                                                                                                         >  1:4373828/1‑135 (MQ=255)
                 aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCATGAGTTTTTATCATAAGc                                                                                                                         >  2:1436005/1‑140 (MQ=255)
                 aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGc                                                                                                                         >  2:3135025/1‑140 (MQ=255)
                 aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTATCTTCAGc                                                                                                                         >  2:5550650/1‑140 (MQ=255)
                 aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTATCATCAGc                                                                                                                         >  1:3178080/1‑140 (MQ=255)
                 aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCAAGAGTTTTTATCATCAGc                                                                                                                         >  2:1229429/1‑140 (MQ=255)
                 aaTACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCAATAGTTTTTCtcaaaagc                                                                                                                         >  1:2715356/1‑135 (MQ=255)
                 aaTACATATGGATCAAGCATCGAAAACAGGCATGGGGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGGTTTTCTCATCAGc                                                                                                                         >  2:835252/1‑140 (MQ=255)
                 aaTACATATCGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACAGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCATGAATTGTtatccaaagc                                                                                                                         >  2:7157129/1‑134 (MQ=255)
                 aaTACATATGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCGGGTACTCTCAGGGGGGAACGAGTCGTTTTTTCCAAGAGTTTTTGtcataaacc                                                                                                                        >  2:248606/1‑135 (MQ=255)
                 aaTACATAGGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGTGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCAAGACTTTTTCtcaacacc                                                                                                                         >  1:2805743/1‑138 (MQ=255)
                       tatGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTTTCCAAGAGTTTTTCTCCAAAGCcatctt                                                                                                                   >  1:5304127/1‑140 (MQ=255)
                        atGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTATCAAAAACCGTCTTg                                                                                                                  >  1:7393193/1‑140 (MQ=255)
                        atGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTCTCATAAGCCTTCTTg                                                                                                                  >  2:3203483/1‑140 (MQ=255)
                          tgATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGc                                                                                                                <  1:4441340/139‑1 (MQ=255)
                              cAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGa                                                                                                            <  1:5661044/140‑1 (MQ=255)
                               aaGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGACCGAGTCGTTTTAGCCCTGAGTTTTTCTCATCAGCCTTCTTGGCCggcg                                                                                                           >  2:5879171/1‑138 (MQ=255)
                                aGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTTGCCATGAGTTTTTATCATAAGCCTTCTTTGCCGGAGt                                                                                                          >  2:1962352/1‑140 (MQ=255)
                                aGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTTGCCATGAGTTTTTATCATAAGCCTTCTTGGCCGGGGt                                                                                                          >  1:3618246/1‑140 (MQ=255)
                                aGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGCGt                                                                                                          >  2:3365793/1‑140 (MQ=255)
                                aGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGt                                                                                                          >  2:4080491/1‑140 (MQ=255)
                                aGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATTAGTTTTTCACATAAGCCTTCTTGGGCGgtgt                                                                                                          >  1:5149950/1‑140 (MQ=255)
                                    tGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTATACTCACCCTTCTTGGCCCGAGTGGGc                                                                                                      >  1:2568206/1‑140 (MQ=255)
                                     ggAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGACCGAGTCGTTTTAGCCATGAGTTTTTCTCATAAGCCTTCTTTGCCGGAGTGGGCa                                                                                                     >  1:2042242/1‑140 (MQ=255)
                                     ggAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCAAGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCa                                                                                                     >  2:660355/1‑140 (MQ=255)
                                      gAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATTAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGGaa                                                                                                    >  1:5434872/1‑140 (MQ=255)
                                      gAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTGTCATCACCGGTCTGGGCCCGAGTGGGCaa                                                                                                    >  2:4976153/1‑140 (MQ=255)
                                      gAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCaa                                                                                                    >  2:2268194/1‑140 (MQ=255)
                                      gAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATAATCCGTCTTGGGCGGCGTGGGcca                                                                                                    >  1:6677081/1‑138 (MQ=255)
                                         aaCAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAc                                                                                                 <  1:7055971/140‑1 (MQ=255)
                                         aaCAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAc                                                                                                 <  1:985676/140‑1 (MQ=255)
                                         aaCAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAc                                                                                                 <  1:3360807/140‑1 (MQ=255)
                                           cAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCa                                                                                               <  1:234571/140‑1 (MQ=255)
                                               cATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTATCATAAGCCATCTTTGCCGGAGTGGGCAAAACCAttta                                                                                           >  1:1642412/1‑139 (MQ=255)
                                                   gTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCAAGAGTTTTTATCAACAGCCTTCTTGGCCCGAGTGGGCAAAACCAATTCAGaa                                                                                       >  2:7045016/1‑140 (MQ=255)
                                                         aaTTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCgg                                                                                 <  1:626668/140‑1 (MQ=255)
                                                           tttGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCa                                                                               <  2:3969397/140‑1 (MQ=255)
                                                           tttGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCa                                                                               <  1:7192478/140‑1 (MQ=255)
                                                              gagaAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAAACATTTAAGAATAGCGGCAttt                                                                            >  2:4813350/1‑140 (MQ=255)
                                                                   gggCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCAt                                                                       <  1:1292556/140‑1 (MQ=255)
                                                                     gCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCAtgt                                                                     <  1:2939655/140‑1 (MQ=255)
                                                                      cAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATTAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATAAtgtt                                                                    >  2:2117633/1‑139 (MQ=255)
                                                                        accGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGtt                                                                  <  2:7581959/138‑1 (MQ=255)
                                                                             gTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACa                                                             <  1:1793795/140‑1 (MQ=255)
                                                                              ttGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTTGCCATTAGTTTTTATCATCAGCCGTCATTGCCGGAGTTGGCAAAAACATTTGAGAATAGCGGCAATTAAAATGTGTTTTACat                                                            >  1:3540353/1‑139 (MQ=255)
                                                                              ttGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTCTAATAACCCTTCGTTGGCGGTGTGGGCAAAACCATTTCAGAAAATCGGCATTTATCATGTGttttagag                                                            >  1:4612928/1‑135 (MQ=255)
                                                                                  gggAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACagagag                                                        <  1:493148/140‑1 (MQ=255)
                                                                                   ggAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGACCGAGTCGTGTTAGCCATGAGTTTTTCTCAGCAGCCGTCGTGGCCGGCGTGGGCAAAACCATATCAGTATAGCCGCAGTTATACTGTGTTTGACAGAGAgg                                                       >  1:7301315/1‑140 (MQ=255)
                                                                                      aGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGc                                                    <  1:6890551/140‑1 (MQ=255)
                                                                                      aGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGc                                                    <  2:2697365/140‑1 (MQ=255)
                                                                                      aGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCAATTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGc                                                    <  1:6482815/140‑1 (MQ=255)
                                                                                       gCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCAATAGTTTTTCTAATCAGCCTTCTTGGCCGGAGTTGGCAAAAACATTTAAGAATAGCCGCATTTATAATTTGTTTGAAAGAGAGGTTCa                                                   >  1:1126417/1‑140 (MQ=255)
                                                                                       gCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTGTGATCAGCCTTCTTGGCCGGCGTGGGCAAAACCATTTCAGAATAGCGGCAATTATCATGTGTTTGACAGAGAGGTGCa                                                   >  2:6733057/1‑140 (MQ=255)
                                                                                       gCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCAAAAGCCGTCGTGGCCGGAGTGGGCAAAAACATTTCAGAATAGCGGCATTTATAAAGTGTTTTAAAGAGAGGTGCa                                                   >  2:6874914/1‑140 (MQ=255)
                                                                                         ggAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACg                                                 <  1:7442171/140‑1 (MQ=255)
                                                                                           agaCAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTTGCCAAGAGTTTTTGTAAAAAACCTTATTGGCCGGAGTGGGGAAAAACAATTAAGAAAAGCGGCATTTATAATGTGTTTGTGAGAGAGGTGCACGAt                                               >  1:1024343/1‑140 (MQ=255)
                                                                                           agaCAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCAAGAGTTTTTATCATCACCCGTCTTGGCCGGGGTGGGCCAAACCATTTCAAGAAACCGGGCTTTTTAATGTGTTTGTCAGGGTCGTGCAccgt                                               >  1:1538944/1‑137 (MQ=255)
                                                                                           agaCAGAGACCGCTGGTACTCTCCGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGAt                                               <  1:5857317/140‑1 (MQ=255)
                                                                                             aCAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCCAGACTTTTTCTCAACACCCATCTTGGCCGGCGGGGGCCAAACCATTTCAGAAGAGCGGCATTTATCATGTGTTTGACAGAGAGGGGCACGCTgg                                             >  1:6011631/1‑139 (MQ=255)
                                                                                              cAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTa                                            <  2:2968445/140‑1 (MQ=255)
                                                                                                  tACCGCTGGTACTCTCAGGGGGGAACGAGTCGTGTTAGCCATGAGTTTTTCTCATCAGCCTTATTGGCCGGCGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGCTGTAAGCa                                        >  2:4556184/1‑140 (MQ=255)
                                                                                                     cGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCAATTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCCAGATGTAATCaaag                                     >  2:1105618/1‑138 (MQ=255)
                                                                                                       cTGGTACTCTCAGGGGGGAACGAGTCGTTTTATCCATGAGTTTTTATCATCACCCTTCTTGGCCGGAGTGGGCAAAACCATTTAAGAAAAGCGGCATTTATAATGTTTTTTACAGATAGGTGCACCATGTAAGCAATaaa                                   >  2:6211410/1‑140 (MQ=255)
                                                                                                       cTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTGTCATCATCCTTCTTGGGCGGCGTGGGCAAAAACATTTCAGAAAATCGGCAGTTATGATGTGTTTGAGAGAGAGGTGCACGATGTTAGCCATGaa                                   >  1:6743796/1‑140 (MQ=255)
                                                                                                       cTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCAATTCAGAATAGCGGCATTTATGATGTGTTTGTCCGAGAGGTGCACGATGTAAGCAATGaa                                   >  2:3550315/1‑140 (MQ=255)
                                                                                                       cTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTATCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAACGaa                                   >  2:1577727/1‑140 (MQ=255)
                                                                                                       cTGGTACTCTCAGGGGGGAACGAGTCGTGTTTGCCAAGAGTTTTTCTCATCAGCCTTCGTGGGCGGAGTGGGGCCAACCATTTCAGAATAGCGGCATTTATAATGTGTTTGTCAGAGAGGTGCACGCTGTGAGCCAGGaa                                   >  1:1415319/1‑140 (MQ=255)
                                                                                                         ggTACTCTCAGGGGGGAACGAGTCGTGTTAGCCAAGAGTTTTTCTCAACAGCCTTCTTGGCCGGAGTGGGCAAAAACAATTAAGAAAATCGGCAATTATGATGTGTTTGACAGAGAGGTGCACCATGTAACCAATGAAAg                                 >  1:7550278/1‑140 (MQ=255)
                                                                                                          gTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGt                                <  1:6371141/140‑1 (MQ=255)
                                                                                                            aCTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTg                                                                <  1:5063220/106‑1 (MQ=255)
                                                                                                             ctctCAGGGGGGAACGAGTCGTTTTAGCCCTGAGTTTTTCTCATAAGCCTTCTTGGCCGGAGTGGGCAAAACCAATTCAGAATAGCCGCCTTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTg                             >  2:5327316/1‑140 (MQ=255)
                                                                                                               ctcAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTGATCACCCGTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATGATGTGTTTGACAGAGAGGTGAAACATGTAAGCAATGAAAGTCTGCa                           >  2:4225068/1‑140 (MQ=255)
                                                                                                                tcAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATAAGCCTTCTTGGCCGGTGTGGGCAAAACCATTTAAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCaa                          >  2:6472555/1‑140 (MQ=255)
                                                                                                                     ggggAACGAGTCGTTTTAGCCCTGAGTTTTTATAATAAGCCTTCTTTGCCGGGGTGGGCAAAAACATTTCAGAAAAGCGGCATTTATCAAGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGc                     >  2:5687489/1‑140 (MQ=255)
                                                                                                                          ccGAGTCGTTTTTCCCATGAGTTTTTCTCATCAGCCGTAATGGGCGGGGTGGGCCAAACAATTTCACCATCGCCGCCTTTTTGATGTGTTTGACAGTGAGGTGCACGATGTAAGCCCTGTAAGTCTGAAAAAGTCAGttt                >  2:5141757/2‑139 (MQ=255)
                                                                                                                          aCGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATATCGGCATTTATCATGTGTTTTGCCGAGAGGTGCACGATGTAAGCAATGAAAGTGTGCAAATGGGAGTtg                >  2:1900717/1‑140 (MQ=255)
                                                                                                                          aCGAGTCGTTTTAGCCATGAGTTTTTCTAATAAGCCTTATTGGCCGGAGTGGGCAAAACCATTTAATAATAGCGGCATTTATGATGTGTTTGAGAGAGAGGTGCACGATGTAAGCAATGAAATTATGCAAATGGGAGTtg                >  2:7439697/1‑140 (MQ=255)
                                                                                                                                cGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTGATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCg          <  1:4221833/140‑1 (MQ=255)
                                                                                                                                  ttttAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCggg        <  1:470462/140‑1 (MQ=255)
                                                                                                                                  ttttAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCggg        <  2:1631520/140‑1 (MQ=255)
                                                                                                                                  ttttAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCggg        <  2:7304878/140‑1 (MQ=255)
                                                                                                                                  ttttAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCggg        <  1:3021723/140‑1 (MQ=255)
                                                                                                                                  ttttAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCggg        <  2:2842706/140‑1 (MQ=255)
                                                                                                                                  ttttAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAAGGAAAGTCTGCAAATGGCAGTTGTGGGCggg        <  1:224485/140‑1 (MQ=255)
                                                                                                                                  ttttAGCCATGAGTTTTTCTCAGCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCggg        <  2:6059003/140‑1 (MQ=255)
                                                                                                                                       gCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGAt   <  2:5632496/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GAGACTGTGCTAGATGAAATACATATGGATCAAGCATGGAAAACAGGCATGGTGACAAATTTGAGAAGGGCAAACGGGTTGTGGGAAGCGGAGACAGATACCGCTGGTACTCTCAGGGGGGAACGAGTCGTTTTAGCCATGAGTTTTTCTCATCAGCCTTCTTGGCCGGAGTGGGCAAAACCATTTCAGAATAGCGGCATTTATCATGTGTTTGACAGAGAGGTGCACGATGTAAGCAATGAAAGTCTGCAAATGGCAGTTGTGGGCGGGGGGATA  >  NZ_CP034943/361213‑361488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: