Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 376,966 A→C 6.6% K781T (AAA→ACA)  srfAA → surfactin non‑ribosomal peptide synthetase SrfAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943376,9660AC 6.6% 1554.9 / 20.3 664K781T (AAA→ACA) srfAAsurfactin non‑ribosomal peptide synthetase SrfAA
Reads supporting (aligned to +/- strand):  ref base A (305/285);  new base C (72/2);  total (377/287)
Fisher's exact test for biased strand distribution p-value = 8.72e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 665 total aligned reads displayed.

TGTTGAAGCTATTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACGGCTGAG  >  NZ_CP034943/376828‑377102
                                                                                                                                          |                                                                                                                                        
      aGCTATTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTg                                                                                                                                   >  2:4511683/1‑140 (MQ=255)
      aGCTATTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTg                                                                                                                                   <  1:6948031/140‑1 (MQ=255)
      aGCTATTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTg                                                                                                                                   <  2:3683757/140‑1 (MQ=255)
      aGCTATTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTg                                                                                                                                   <  1:4054564/140‑1 (MQ=255)
          aTTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAg                                                                                                                                        <  1:3665084/131‑1 (MQ=255)
          aTTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGGGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGACAGCGCTGGGGa                                                                                                                               >  2:2353160/1‑140 (MQ=255)
            ttAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAAt                                                                                                                             <  2:2080164/140‑1 (MQ=255)
            ttAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAAt                                                                                                                             <  2:5872577/140‑1 (MQ=255)
            ttAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATCTGTACGGGCCGACTGACACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCCCTGGGGAAt                                                                                                                             >  2:5048654/1‑140 (MQ=255)
             tAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATa                                                                                                                            <  1:45065/140‑1 (MQ=255)
                     cAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCACTCAGCGTATTTGCCGATCGGGAAAGCGCTGGGGAATAACCGTGta                                                                                                                    >  1:4647808/1‑140 (MQ=255)
                     cAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGACCGCGCTGGGGAATAACCGTTta                                                                                                                    >  1:1002845/1‑140 (MQ=255)
                     cAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTta                                                                                                                    >  1:2058557/1‑140 (MQ=255)
                             gcgcCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGACAGCGCTGGGGAATAACCGTTTATATAtttt                                                                                                            >  2:7185408/1‑140 (MQ=255)
                             gcgcCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTg                                                                                                                                   >  2:4068227/1‑117 (MQ=255)
                              cgccgcGTTTGACCAATGTGTACGGGCCGACTGAAAGGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTg                                                                                                           <  1:5046869/140‑1 (MQ=255)
                              cgccgcGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGCCAGCGCTGGGGAATAACCGTTTATATATTTTg                                                                                                           >  1:4344302/1‑140 (MQ=255)
                              cgccgcGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTg                                                                                                           >  2:3918836/1‑140 (MQ=255)
                              cgccgcGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTg                                                                                                           >  1:4892387/1‑140 (MQ=255)
                                 cgcgTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGAt                                                                                                        <  1:3551821/140‑1 (MQ=255)
                                   cgTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTGATATATTTTGGATCa                                                                                                      >  1:75663/1‑140 (MQ=255)
                                   cgTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGGCTGTGGCGATCGGGACAGCGCTGGGGAATAACCGTTTAGATATTTTGGATCa                                                                                                      >  2:7299764/1‑140 (MQ=255)
                                        gACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGACTAACCGTTTATATATTTTGGATCAAagag                                                                                                 >  1:3325836/1‑140 (MQ=255)
                                        gACCAATGTGTACGGGCCGACTGAAACGTGTGGTGACGCGTCCGTTCAGCAGGTTATCCCAGAGAGTGCCGTTCACTCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGACTACCCGTGGATATATGTTGGATCAAagag                                                                                                 >  1:597976/1‑140 (MQ=255)
                                             aTGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGaggcagg                                                                                            <  1:749206/140‑1 (MQ=255)
                                              tgtgTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGACTAACCGTTTATATATTTTGGATCAAAGaggcaggc                                                                                           >  2:7123443/1‑140 (MQ=255)
                                              tgtgTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGaggcaggc                                                                                           >  1:5764469/1‑140 (MQ=255)
                                                tgtACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCTGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTg                                                                                         <  1:934480/140‑1 (MQ=255)
                                                tgtACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTg                                                                                         <  2:673976/140‑1 (MQ=255)
                                                  tACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGACAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCa                                                                                       >  2:4887008/1‑140 (MQ=255)
                                                       gCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTCTGTGCCGATCGGGACAGCGCTGGGGAATAACCGTTTATATATTTTGGCTCAAAGAGGCAGGCTGCAGCCgg                                                                                  >  2:6099549/1‑140 (MQ=255)
                                                       gCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTCTGTGCCGATCGGGAAAGCGCTGGGGAATCACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCgg                                                                                  >  2:4093784/1‑140 (MQ=255)
                                                       gCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAACGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCgg                                                                                  >  2:5666255/1‑140 (MQ=255)
                                                       gCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCgg                                                                                  >  2:5171432/1‑140 (MQ=255)
                                                               aaaCGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGtgtg                                                                          >  1:4734658/1‑140 (MQ=255)
                                                               aaaCGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGAGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGtgtg                                                                          >  1:7014448/1‑140 (MQ=255)
                                                                aaCGTGTGTTGACGCGTCCGTTCATCAGGTTATCCGAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGTGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTgg                                                                         <  1:2195959/140‑1 (MQ=255)
                                                                     gtgtTGACGCGTCCGTTCATCAGGTTATCCCCGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTGTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTggcgggc                                                                    <  1:2526726/140‑1 (MQ=255)
                                                                     gtgtTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTggcgggc                                                                    <  1:996553/140‑1 (MQ=255)
                                                                     gtgtTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTggcgggc                                                                    <  2:4357927/140‑1 (MQ=255)
                                                                     gtgtTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTggcgggc                                                                    <  2:3802260/140‑1 (MQ=255)
                                                                     gtgtTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTggcgggc                                                                    <  1:1887010/140‑1 (MQ=255)
                                                                     gtgtTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTggcgggc                                                                    <  1:1249374/140‑1 (MQ=255)
                                                                        gtGAAGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAg                                                                 <  1:6694019/139‑1 (MQ=255)
                                                                          gtcgcgTCCGTTCATCAGTTTTTCCCTGACAGTGCCGTTCTATAATCGTATGTGCCGTTCGGGAAAGCGGTGGGGATTTACCGTTTATATATTTTGGATCAATGAGGCATTCTGCAGACGGAAGGTTTGGAGGGCGTGCt                                                               <  2:7602586/138‑1 (MQ=255)
                                                                          gACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCt                                                               <  2:4605700/140‑1 (MQ=255)
                                                                           aCGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCtt                                                              >  2:4768141/1‑140 (MQ=255)
                                                                                tCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCCGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTatat                                                         >  1:3147686/1‑140 (MQ=255)
                                                                                tCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAACGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTatat                                                         >  1:3829603/1‑140 (MQ=255)
                                                                                    ttactcaGGTTATCCCACAGCGTGCCGTTCAATCAGCGTATGTGCCGATCGGGACAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCACCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCa                                                     <  2:5231958/136‑1 (MQ=255)
                                                                                    ttCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCa                                                     <  2:2904093/140‑1 (MQ=255)
                                                                                      catcaGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAgg                                                   <  1:7681893/140‑1 (MQ=255)
                                                                                         caGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                <  2:2705105/140‑1 (MQ=255)
                                                                                         caGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                <  1:1586179/140‑1 (MQ=255)
                                                                                            gTTATCCCAGAGAGTGCCGTTCAATCATAGTATGTGACGAACGGGAAAGCGCTGGTGAATAAAAGTTTAGATATTTTCTATCAAAGAGGCATACTTCAGCCGGAATGTTTGGCGGGCGAGCTTTATATCTCAGGCGACgg                                             >  2:172974/1‑140 (MQ=255)
                                                                                            gTTATCCCAGAGAGTGCCGTTCAATCAGCGTCTGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGCCGACgg                                             >  2:2214026/1‑140 (MQ=255)
                                                                                              tATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGgtg                                           <  2:4940146/140‑1 (MQ=255)
                                                                                              tATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGgtg                                           <  2:856813/140‑1 (MQ=255)
                                                                                              tATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGgtg                                           <  2:6982212/140‑1 (MQ=255)
                                                                                                 cccAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAACGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTg                                        >  2:6446725/1‑140 (MQ=255)
                                                                                                 cccAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTg                                        >  2:3263473/1‑140 (MQ=255)
                                                                                                   cAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGc                                      <  2:6628869/140‑1 (MQ=255)
                                                                                                   cAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGc                                      <  2:1949017/140‑1 (MQ=255)
                                                                                                   cAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGc                                      <  1:7111608/140‑1 (MQ=255)
                                                                                                   cAGAGAGTGCCCTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGc                                      <  1:6109945/140‑1 (MQ=255)
                                                                                                      agagTGCCGTTCAATCAGCGTCTGTGCCGATCGGGAAAGCGCTGGGGAATAACCGGTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGa                                   >  1:5274004/1‑140 (MQ=255)
                                                                                                      agagTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGACTAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGa                                   >  1:2739841/1‑140 (MQ=255)
                                                                                                      agagTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCg                                    <  1:6362412/139‑1 (MQ=255)
                                                                                                       gagTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAg                                  <  2:6341259/140‑1 (MQ=255)
                                                                                                        agTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAgg                                 <  1:2452488/140‑1 (MQ=255)
                                                                                                          tGCCGTTCAATCAGCGTATGTGCCGATCGGGACAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCt                               >  2:3144020/1‑140 (MQ=255)
                                                                                                          tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                <  1:3431600/123‑1 (MQ=255)
                                                                                                          tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGc                                >  2:1165235/1‑139 (MQ=255)
                                                                                                          tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCt                               >  2:7491742/1‑140 (MQ=255)
                                                                                                          tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCt                               >  1:5400291/1‑140 (MQ=255)
                                                                                                          tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGGTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCt                               >  2:3583997/1‑140 (MQ=255)
                                                                                                               ttCAATCAGCGTCTGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTa                          >  2:6857269/1‑140 (MQ=255)
                                                                                                               ttCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTa                          >  1:6777251/1‑140 (MQ=255)
                                                                                                                 cAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACa                        <  2:1286851/140‑1 (MQ=255)
                                                                                                                  aaTCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACAt                       >  2:249903/1‑140 (MQ=255)
                                                                                                                    tCAGCGTATGTGCCGATCGGGACAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACAttt                     >  1:6500166/1‑140 (MQ=255)
                                                                                                                       gCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGcc                  >  1:2222315/1‑140 (MQ=255)
                                                                                                                        cGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCt                 <  1:1490575/140‑1 (MQ=255)
                                                                                                                        cGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCt                 <  2:2998238/140‑1 (MQ=255)
                                                                                                                         gTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGGTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTg                >  2:5817962/1‑140 (MQ=255)
                                                                                                                          tATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGa               >  2:5483224/1‑140 (MQ=255)
                                                                                                                          tATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGa               >  1:7322571/1‑140 (MQ=255)
                                                                                                                            tgtgCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAAt             >  2:1606432/1‑140 (MQ=255)
                                                                                                                              tgCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTg           >  2:5318526/1‑140 (MQ=255)
                                                                                                                              tgCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTg           >  1:71815/1‑140 (MQ=255)
                                                                                                                                ccGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGAc         >  1:1339797/1‑140 (MQ=255)
                                                                                                                                ccGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGAc         >  1:174592/1‑140 (MQ=255)
                                                                                                                                  gATCGGGACAGCGCTGGGGACTAACCGTTTATATATTTTGGATCAAATAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg       >  2:1370740/1‑140 (MQ=255)
                                                                                                                                  gATCGGGACAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg       >  2:3430876/1‑140 (MQ=255)
                                                                                                                                  gATCGGGAACGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg       >  1:4194074/1‑140 (MQ=255)
                                                                                                                                  gATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                >  1:308022/1‑99 (MQ=255)
                                                                                                                                  gATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg       >  1:2893064/1‑140 (MQ=255)
                                                                                                                                  gATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg       >  1:5588725/1‑140 (MQ=255)
                                                                                                                                  gATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg       >  1:6844853/1‑140 (MQ=255)
                                                                                                                                      gggAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACGGCTga   <  2:1536160/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TGTTGAAGCTATTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACGGCTGAG  >  NZ_CP034943/376828‑377102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: